Difference between revisions of "Os08g0540400"

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''OsCPK21'', is a mumber of CDPKs (calcium-dependent protein kinases), which play important roles in regulating downstream components in calcium signaling pathways<ref name="ref1"/>.
  
 
==Annotated Information==
 
==Annotated Information==
 
===Function===
 
===Function===
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Please input function information here.
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*''OsCPK21'' is involved in the positive regulation of the signaling pathways that are involved in the response to ABA and salt stress.
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Calcium plays an important role as a messenger in various signal transduction pathways. Calcium-dependent protein kinases (CDPKs) are Ca<sup>2+</sup>-binding sensory kinases that regulate the downstream components of calcium signaling. CDPKs have been identified throughout the plant kingdom and in some protozoans but not in animals<ref name="ref1"/>.
 +
*Overexpression of ''OsCPK21'' in rice confers increased sensitivity to exogenous ABA, enhanced tolerance to salinity stress and elevated expression of ABA- and stress-inducible genes, suggesting a role for ''OsCPK21'' as a positive factor in the ABA and salt stress
 +
signaling pathways. These findings could contribute to the elucidation of the CDPK-mediated ABA and salt stress signaling and to generate crops with improved tolerance to salt stress<ref name="ref1"/>.
 +
 
 +
'''GO assignment(s):''' [http://amigo.geneontology.org/amigo/term/GO:0004672 GO:0004672], [http://amigo.geneontology.org/amigo/term/GO:0004674 GO:0004674], [http://amigo.geneontology.org/amigo/term/GO:0006468 GO:0006468], [http://amigo.geneontology.org/amigo/term/GO:0005524 GO:0005524], [http://amigo.geneontology.org/amigo/term/GO:0005509 GO:0005509]
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===Mutation===
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WT plants VS ''OsCPK21''-FOX plants<ref name="ref1"/>:
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Using a mini-scale full-length cDNA overexpressor (FOX) gene hunting system, ''Asano et al.'' generated 250 independent transgenic
 +
rice plants overexpressing individual rice CDPKs(CDPK FOX-rice lines).These CDPK FOX-rice lines were screened for salt stress tolerance. The survival rate of the ''OsCPK21''-FOX plants was higher than that of wild-type(WT) plants grown under high salinity conditions. The
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inhibition of seedling growth by abscisic acid (ABA) treatment was greater in the ''OsCPK21''-FOX plants than in WT plants. Several ABA- and high salinity-inducible genes were more highly expressed in the ''OsCPK21''-FOX plants than in WT plants.  
  
 
===Expression===
 
===Expression===
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Please input expression information here.
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[[File: OsCPK21-FOX and OsCPK13-1-FOX.jpg|left|thumb|250px|'''Figure 1.'''''Tolerance of OsCPK21-FOX and OsCPK13-1-FOX plants to salt stress.(from reference <ref name="ref1"/>).'']]
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[[File: OsCPK21 Phylogenetic1.jpg|right|thumb|300px|'''Figure 2.'''''Phylogenetic relationships between CDPKs from rice and Arabidopsis.(from reference <ref name="ref1"/>).'']]
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*RT–PCR analysis confirmed that the ''OsCPK21'' and ''OsCPK13-1'' cDNAs were overexpressed in the ''OsCPK21''- and the ''OsCPK13-1''-FOX plants compared to WT(Fig. 1a). The ''OsCPK21''-FOX plants also showed greater salt tolerance than WT plants (Fig. 1b). The survival rates of the ''OsCPK21''-FOX plants under high salinity conditions reached 61–70%, whereas the survival of the WT plants was only 22% (Fig. 1c). No obvious differences in growth or development were observed between the transgenic and WT plants grown under normal growth conditions (data not shown). The survival rates of the OsCPK13-1-FOX plants under high salinity conditions were higher than those of
 +
WT plants (Fig. 1c), and this result is consistent with that of ''Saijo et al.''<ref name="ref1"/><ref name="ref2"/>.
 +
*''Asano et al.'' showed that ''OsCPK21'' was strongly expressed in developing seeds<ref name="ref3"/>. RT–PCR was used to monitor the expression of ''OsCPK21'' in different tissues, and we detected high levels of expression in spikelets and developing seeds. RT–PCR analyses showed that the expression of ''OsCPK21'' rapidly increased in the high salinity treatment, with the highest level recorded at 5 h. A relatively slow increase in OsCPK21 transcripts was also observed after ABA treatment. the abovementioned DRE, MYB and MYC recognition sequences may function as cis-acting elements responsive to abiotic stress in the ''OsCPK21'' promoter.
 +
*''Asano et al.'' analysed the expression of ABA- and stressinducible genes in the ''OsCPK13-1''-FOX plants compared to WT. When the plants were treated with exogenous ABA, the expression levels of the ''OsP5CS'', ''OsLEA3'' and ''OsNAC6'' genes were more highly elevated in the ''OsCPK13-1''-FOX plants than in WT plants. Expression of ''OsP5CS'', ''OsLEA3'', ''OsNAC6'', ''OsbZIP23'', ''OsNHX1'' and ''OsSOS1'' was also clearly enhanced by salt stress in the ''OsCPK13-1''-FOX plants compared to WT<ref name="ref1"/>.
  
 
===Evolution===
 
===Evolution===
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Please input evolution information here.
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[[File: OsCPK21 Phylogenetic2.jpg|right|thumb|300px|'''Figure 3.Phylogenetic relatedness among the rice, Arabidopsis and functionally characterized CDPKs from other plant species.(from reference <ref name="ref4"/>).'']]
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[[File: OsCPK21 Phylogenetic3.jpg|left|thumb|300px|'''Figure 4.'''''Phylogenetic relationships among CDPKs from rice (OsCPK1-OsCPK29)
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and Arabidopsis (AtCPK1-AtCPK34).(from reference <ref name="ref5"/>).'']]
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[[File: OsCPK21 Phylogenetic4.jpg|right|thumb|250px|'''Figure 5.Phylogenetic relationships among rice CCaMK, and rice and Arabidopsis
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CDPKs, CRKs and PEPRKs.(from reference <ref name="ref3"/>).'']]
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*The phylogenetic tree was created using the ClustalW program based on the alignment of the kinase catalytic domains of 29 rice (''OsCPK1''-''OsCPK29'') and 34 ''Arabidopsis'' (''AtCPK1''-''AtCPK34'') CDPKs. ''OsCPK21'' is indicated by an arrow. Phylogenetic analysis showed that rice CDPKs are divided into four distinct classes<ref name="ref1"/>(Fig. 2).
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*To study the evolutionary relatedness of rice and ''Arabidopsis'' CDPKs with all the CDPK genes characterized so far from alfalfa, cucumber, ice plant, mung bean, potato, strawberry, tomato, ''Petunia'', maize, tobacco and ''Medicago'', an unrooted tree was constructed by using ClustalX 1.83. This exercise resulted in four distinct groups similar to that reported by ''Asano et al.''<ref name="ref3"/><ref name="ref4"/>(Fig. 3).
 +
*The amplitude of difierential expression for these genes was not as significant as reported earlier, possibly due to use of difierent rice variety and/or experimental conditions. Most of the previously identiWed stress responsive CDPK genes cluster together in
 +
subclades Ia and Ib<ref name="ref4"/>(Fig. 3).
 +
*Each calcium-dependent protein kinase (CDPK) consists of a variable N-terminal domain, a protein kinase domain, an autoinhibitory region and a calmodulin-like domain with EFhand Ca<sup>2+</sup>-binding sites. CDPKs are directly activated by the binding of Ca<sup>2+</sup> to the calmodulin-like domain, and the activated CDPKs regulate downstream targets. CDPKs have been identified throughout the plant kingdom， and in some protozoans, but not in animals. CDPKs constitute a large multigene family in various plant species; CDPK genes have been identified in ''Arabidopsis thaliana'', and CDPK genes have been found in ''Oryza sativa'' (rice)18 (Fig. 4). The expression and activities of CDPKs are upregulated by a variety of stimuli, such as hormones, abiotic stresses and biotic stresses. Red letters indicate CDPKs involved in abiotic stress signaling<ref name="ref5"/>.
 +
*Phylogenetic relationships among rice CCaMK, and rice and ''Arabidopsis'' CDPKs, CRKs and PEPRKs. A phylogenetic tree was created using the ClustalW program, based on the predicted amino acid sequences of the rice and ''Arabidopsis'' kinases, which are indicated by red and blue type, respectively.As shown in Fig. 5, the phylogenetic tree of these kinase sequences forms seven subgroups: CDPKs I–IV, CRKs, CCaMK and PEPRKs. Furthermore, the 29 rice CDPKs were divided into four distinct classes<ref name="ref3"/>.
 +
 
 +
===Knowledge Extension===
 +
[[File: function of CDPKs.jpg|left|thumb|250px|'''Figure 6.Summary of the function of CDPKs in ABA and abiotic stress responses, as reported by several authors（from reference <ref name="ref5"/>).'']]
 +
In rice, the CDPKs constitute a large family of 29 genes<ref name="ref3"/>.
 +
CDPK genes (''OsCPK1-29'') contain multiple stress-responsive cis-elements in the promoter region (1 kb) upstream of genes. Analysis of the information extracted from the Rice Expression Database indicates that 11 of the CDPK genes are regulated by chilling temperature, dehydration, salt, rice blast infection and chitin treatment. RT-PCR and RNA gel blot hybridization were performed in this study to detect the expression 19 of the CDPK genes. Twelve CDPK genes exhibited cultivar- and tissue-specific expression; four CDPK genes (''OsCPK6'', ''OsCPK13'', ''OsCPK17'' and ''OsCPK25'') were induced by chilling temperature, dehydration and salt stresses in the rice seedlings. While ''OsCPK13'' (''OsCDPK7'')<ref name="ref2"/> was already known to be inducible by chilling temperature and high salt, this is the first report that the other three genes are stress-regulated. ''OsCPK6'' and ''OsCPK25'' are up-regulated by dehydration and heat shock, respectively, while ''OsCPK17'' is down-regulated by chilling temperature, dehydration and high salt stresses. Based on this evidence, rice CDPK genes may be important components in the signal transduction pathways for stress responses<ref name="ref1"/><ref name="ref6"/>.
 +
 
 +
Three major classes of Ca<sup>2+</sup>-binding proteins have been characterized in higher plants: calciumdependent protein kinases (CDPKs), calmodulins (CaMs) and CaM-like proteins, and calcineurin B-like proteins<ref name="ref6"/>.
  
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You can also add sub-section(s) at will.
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The subcellular localization of each CDPK and the phenotypes of overexpression (OX) or knockout or knockdown (KO) lines are described. Red letters, ''Arabidopsis'' CDPKs; green letters, rice CDPKs; P, phosphorylation; ABF, ABA-responsive element binding factor; HSP1, a heat shock protein; OST1, open stomata 1 protein kinase<ref name="ref5"/>(Fig. 6).
  
 
==Labs working on this gene==
 
==Labs working on this gene==
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Please input related labs here.
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*Department of Agricultural and Environmental Biology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan
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*National Institute of Agrobiological Sciences, 2-1-2 Kannondai, Tsukuba, Ibaraki 305-8602, Japan
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*National Agricultural Research Center, Joetsu, Niigata 943-0193, Japan
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*Department of Applied Biological Chemistry, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan
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*National Institute of Crop Science, Tsukuba, Ibaraki 305-8518, Japan
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*National Key Laboratory of Crop, Genetic Improvement and National Centre of Plant Gene Research, Huazhong Agricultural University, Wuhan 430070, PR China
 +
*Interdisciplinary Centre for Plant Genomics and Department of Plant Molecular Biology, University of Delhi South Campus, Benito Juarez Road, New Delhi 110021, India
  
 
==References==
 
==References==
−
Please input cited references here.
+
<references>
 +
* <ref name="ref1">
 +
Asano T, Hakata M, Nakamura H, et al. Functional characterisation of OsCPK21, a calcium-dependent protein kinase that confers salt tolerance in rice[J]. Plant molecular biology, 2011, 75(1-2): 179-191.
 +
</ref>
 +
* <ref name="ref2">
 +
Saijo Y, Hata S, Kyozuka J, et al. Over‐expression of a single Ca2+‐dependent protein kinase confers both cold and salt/drought tolerance on rice plants[J]. The Plant Journal, 2000, 23(3): 319-327.
 +
</ref>
 +
* <ref name="ref3">
 +
Asano T, Tanaka N, Yang G, et al. Genome-wide identification of the rice calcium-dependent protein kinase and its closely related kinase gene families: comprehensive analysis of the CDPKs gene family in rice[J]. Plant and cell physiology, 2005, 46(2): 356-366.
 +
</ref>
 +
* <ref name="ref4">
 +
Ray S, Agarwal P, Arora R, et al. Expression analysis of calcium-dependent protein kinase gene family during reproductive development and abiotic stress conditions in rice (Oryza sativa L. ssp. indica)[J]. Molecular Genetics and Genomics, 2007, 278(5): 493-505.
 +
</ref>
 +
* <ref name="ref5">
 +
Asano T, Hayashi N, Kikuchi S, et al. CDPK-mediated abiotic stress signaling[J]. Plant Signal Behav, 2012, 7(7): 817-821.
 +
</ref>
 +
* <ref name="ref6">
 +
Wan B, Lin Y, Mou T. Expression of rice Ca< sup> 2+</sup>-dependent protein kinases (CDPKs) genes under different environmental stresses[J]. FEBS letters, 2007, 581(6): 1179-1189.
 +
</ref>
 +
</references>
  
 
==Structured Information==
 
==Structured Information==

Revision as of 04:46, 5 January 2015

OsCPK21, is a mumber of CDPKs (calcium-dependent protein kinases), which play important roles in regulating downstream components in calcium signaling pathways[1].

Annotated Information

Function

  • OsCPK21 is involved in the positive regulation of the signaling pathways that are involved in the response to ABA and salt stress.

Calcium plays an important role as a messenger in various signal transduction pathways. Calcium-dependent protein kinases (CDPKs) are Ca2+-binding sensory kinases that regulate the downstream components of calcium signaling. CDPKs have been identified throughout the plant kingdom and in some protozoans but not in animals[1].

  • Overexpression of OsCPK21 in rice confers increased sensitivity to exogenous ABA, enhanced tolerance to salinity stress and elevated expression of ABA- and stress-inducible genes, suggesting a role for OsCPK21 as a positive factor in the ABA and salt stress

signaling pathways. These findings could contribute to the elucidation of the CDPK-mediated ABA and salt stress signaling and to generate crops with improved tolerance to salt stress[1].

GO assignment(s): GO:0004672, GO:0004674, GO:0006468, GO:0005524, GO:0005509

Mutation

WT plants VS OsCPK21-FOX plants[1]: Using a mini-scale full-length cDNA overexpressor (FOX) gene hunting system, Asano et al. generated 250 independent transgenic rice plants overexpressing individual rice CDPKs(CDPK FOX-rice lines).These CDPK FOX-rice lines were screened for salt stress tolerance. The survival rate of the OsCPK21-FOX plants was higher than that of wild-type(WT) plants grown under high salinity conditions. The inhibition of seedling growth by abscisic acid (ABA) treatment was greater in the OsCPK21-FOX plants than in WT plants. Several ABA- and high salinity-inducible genes were more highly expressed in the OsCPK21-FOX plants than in WT plants.

Expression

Figure 1.Tolerance of OsCPK21-FOX and OsCPK13-1-FOX plants to salt stress.(from reference [1]).
Figure 2.Phylogenetic relationships between CDPKs from rice and Arabidopsis.(from reference [1]).
  • RT–PCR analysis confirmed that the OsCPK21 and OsCPK13-1 cDNAs were overexpressed in the OsCPK21- and the OsCPK13-1-FOX plants compared to WT(Fig. 1a). The OsCPK21-FOX plants also showed greater salt tolerance than WT plants (Fig. 1b). The survival rates of the OsCPK21-FOX plants under high salinity conditions reached 61–70%, whereas the survival of the WT plants was only 22% (Fig. 1c). No obvious differences in growth or development were observed between the transgenic and WT plants grown under normal growth conditions (data not shown). The survival rates of the OsCPK13-1-FOX plants under high salinity conditions were higher than those of

WT plants (Fig. 1c), and this result is consistent with that of Saijo et al.[1][2].

  • Asano et al. showed that OsCPK21 was strongly expressed in developing seeds[3]. RT–PCR was used to monitor the expression of OsCPK21 in different tissues, and we detected high levels of expression in spikelets and developing seeds. RT–PCR analyses showed that the expression of OsCPK21 rapidly increased in the high salinity treatment, with the highest level recorded at 5 h. A relatively slow increase in OsCPK21 transcripts was also observed after ABA treatment. the abovementioned DRE, MYB and MYC recognition sequences may function as cis-acting elements responsive to abiotic stress in the OsCPK21 promoter.
  • Asano et al. analysed the expression of ABA- and stressinducible genes in the OsCPK13-1-FOX plants compared to WT. When the plants were treated with exogenous ABA, the expression levels of the OsP5CS, OsLEA3 and OsNAC6 genes were more highly elevated in the OsCPK13-1-FOX plants than in WT plants. Expression of OsP5CS, OsLEA3, OsNAC6, OsbZIP23, OsNHX1 and OsSOS1 was also clearly enhanced by salt stress in the OsCPK13-1-FOX plants compared to WT[1].

Evolution

'Figure 3.Phylogenetic relatedness among the rice, Arabidopsis and functionally characterized CDPKs from other plant species.(from reference [4]).
Figure 4.Phylogenetic relationships among CDPKs from rice (OsCPK1-OsCPK29) and Arabidopsis (AtCPK1-AtCPK34).(from reference [5]).
'Figure 5.Phylogenetic relationships among rice CCaMK, and rice and Arabidopsis CDPKs, CRKs and PEPRKs.(from reference [3]).
  • The phylogenetic tree was created using the ClustalW program based on the alignment of the kinase catalytic domains of 29 rice (OsCPK1-OsCPK29) and 34 Arabidopsis (AtCPK1-AtCPK34) CDPKs. OsCPK21 is indicated by an arrow. Phylogenetic analysis showed that rice CDPKs are divided into four distinct classes[1](Fig. 2).
  • To study the evolutionary relatedness of rice and Arabidopsis CDPKs with all the CDPK genes characterized so far from alfalfa, cucumber, ice plant, mung bean, potato, strawberry, tomato, Petunia, maize, tobacco and Medicago, an unrooted tree was constructed by using ClustalX 1.83. This exercise resulted in four distinct groups similar to that reported by Asano et al.[3][4](Fig. 3).
  • The amplitude of difierential expression for these genes was not as significant as reported earlier, possibly due to use of difierent rice variety and/or experimental conditions. Most of the previously identiWed stress responsive CDPK genes cluster together in

subclades Ia and Ib[4](Fig. 3).

  • Each calcium-dependent protein kinase (CDPK) consists of a variable N-terminal domain, a protein kinase domain, an autoinhibitory region and a calmodulin-like domain with EFhand Ca2+-binding sites. CDPKs are directly activated by the binding of Ca2+ to the calmodulin-like domain, and the activated CDPKs regulate downstream targets. CDPKs have been identified throughout the plant kingdom， and in some protozoans, but not in animals. CDPKs constitute a large multigene family in various plant species; CDPK genes have been identified in Arabidopsis thaliana, and CDPK genes have been found in Oryza sativa (rice)18 (Fig. 4). The expression and activities of CDPKs are upregulated by a variety of stimuli, such as hormones, abiotic stresses and biotic stresses. Red letters indicate CDPKs involved in abiotic stress signaling[5].
  • Phylogenetic relationships among rice CCaMK, and rice and Arabidopsis CDPKs, CRKs and PEPRKs. A phylogenetic tree was created using the ClustalW program, based on the predicted amino acid sequences of the rice and Arabidopsis kinases, which are indicated by red and blue type, respectively.As shown in Fig. 5, the phylogenetic tree of these kinase sequences forms seven subgroups: CDPKs I–IV, CRKs, CCaMK and PEPRKs. Furthermore, the 29 rice CDPKs were divided into four distinct classes[3].

Knowledge Extension

'Figure 6.Summary of the function of CDPKs in ABA and abiotic stress responses, as reported by several authors（from reference [5]).

In rice, the CDPKs constitute a large family of 29 genes[3]. CDPK genes (OsCPK1-29) contain multiple stress-responsive cis-elements in the promoter region (1 kb) upstream of genes. Analysis of the information extracted from the Rice Expression Database indicates that 11 of the CDPK genes are regulated by chilling temperature, dehydration, salt, rice blast infection and chitin treatment. RT-PCR and RNA gel blot hybridization were performed in this study to detect the expression 19 of the CDPK genes. Twelve CDPK genes exhibited cultivar- and tissue-specific expression; four CDPK genes (OsCPK6, OsCPK13, OsCPK17 and OsCPK25) were induced by chilling temperature, dehydration and salt stresses in the rice seedlings. While OsCPK13 (OsCDPK7)[2] was already known to be inducible by chilling temperature and high salt, this is the first report that the other three genes are stress-regulated. OsCPK6 and OsCPK25 are up-regulated by dehydration and heat shock, respectively, while OsCPK17 is down-regulated by chilling temperature, dehydration and high salt stresses. Based on this evidence, rice CDPK genes may be important components in the signal transduction pathways for stress responses[1][6].

Three major classes of Ca2+-binding proteins have been characterized in higher plants: calciumdependent protein kinases (CDPKs), calmodulins (CaMs) and CaM-like proteins, and calcineurin B-like proteins[6].

The subcellular localization of each CDPK and the phenotypes of overexpression (OX) or knockout or knockdown (KO) lines are described. Red letters, Arabidopsis CDPKs; green letters, rice CDPKs; P, phosphorylation; ABF, ABA-responsive element binding factor; HSP1, a heat shock protein; OST1, open stomata 1 protein kinase[5](Fig. 6).

Labs working on this gene

  • Department of Agricultural and Environmental Biology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan
  • National Institute of Agrobiological Sciences, 2-1-2 Kannondai, Tsukuba, Ibaraki 305-8602, Japan
  • National Agricultural Research Center, Joetsu, Niigata 943-0193, Japan
  • Department of Applied Biological Chemistry, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan
  • National Institute of Crop Science, Tsukuba, Ibaraki 305-8518, Japan
  • National Key Laboratory of Crop, Genetic Improvement and National Centre of Plant Gene Research, Huazhong Agricultural University, Wuhan 430070, PR China
  • Interdisciplinary Centre for Plant Genomics and Department of Plant Molecular Biology, University of Delhi South Campus, Benito Juarez Road, New Delhi 110021, India

References

  1. ↑ 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 1.8 1.9 Asano T, Hakata M, Nakamura H, et al. Functional characterisation of OsCPK21, a calcium-dependent protein kinase that confers salt tolerance in rice[J]. Plant molecular biology, 2011, 75(1-2): 179-191.
  2. ↑ 2.0 2.1 Saijo Y, Hata S, Kyozuka J, et al. Over‐expression of a single Ca2+‐dependent protein kinase confers both cold and salt/drought tolerance on rice plants[J]. The Plant Journal, 2000, 23(3): 319-327.
  3. ↑ 3.0 3.1 3.2 3.3 3.4 Asano T, Tanaka N, Yang G, et al. Genome-wide identification of the rice calcium-dependent protein kinase and its closely related kinase gene families: comprehensive analysis of the CDPKs gene family in rice[J]. Plant and cell physiology, 2005, 46(2): 356-366.
  4. ↑ 4.0 4.1 4.2 Ray S, Agarwal P, Arora R, et al. Expression analysis of calcium-dependent protein kinase gene family during reproductive development and abiotic stress conditions in rice (Oryza sativa L. ssp. indica)[J]. Molecular Genetics and Genomics, 2007, 278(5): 493-505.
  5. ↑ 5.0 5.1 5.2 5.3 Asano T, Hayashi N, Kikuchi S, et al. CDPK-mediated abiotic stress signaling[J]. Plant Signal Behav, 2012, 7(7): 817-821.
  6. ↑ 6.0 6.1 Wan B, Lin Y, Mou T. Expression of rice Ca< sup> 2+</sup>-dependent protein kinases (CDPKs) genes under different environmental stresses[J]. FEBS letters, 2007, 581(6): 1179-1189.

Structured Information

Gene Name

Os08g0540400

Description

Similar to Calcium-dependent protein kinase

Version

NM_001068918.1 GI:115477574 GeneID:4346187

Length

4244 bp

Definition

Oryza sativa Japonica Group Os08g0540400, complete gene.

Source

Oryza sativa Japonica Group 

 ORGANISM  Oryza sativa Japonica Group
           Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
           Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
           clade; Ehrhartoideae; Oryzeae; Oryza.
Chromosome

Chromosome 8

Location

Chromosome 8:27143096..27147339

Sequence Coding Region

27143302..27143655,27144921..27145088,27145174..27145289,27145400..27145552,27146097..27146238
,27146333..27146458,27146579..27147217

Expression

GEO Profiles:Os08g0540400

Genome Context

<gbrowseImage1> name=NC_008401:27143096..27147339 source=RiceChromosome08 preset=GeneLocation </gbrowseImage1>

Gene Structure

<gbrowseImage2> name=NC_008401:27143096..27147339 source=RiceChromosome08 preset=GeneLocation </gbrowseImage2>

Coding Sequence

<cdnaseq>atggggggctgctactccgcctacgcctcctcgcgcaagctgcgcggccgcatcagcaagatctccctcgtcatccccgacccggtccccgacgccgaggccgcctcgccgcgcaaggacggcgtcgatggcgacggcgacgacgtgaggggtggtggtggtggctgtgacgatggcggtgacgtcgtcgccatcgcgacgacgacggcggacgagttcgcgcggcggtacgtgctggggaaggagctggggcgcggggagttcggggtgacgcggcggtgcagcgacgcggcgaccggggaggcgctggcgtgcaagacgatccggaagcacaggcgcctggcgccgccgcgggtgaccgcggcgaaggcggcggccgcgcacggggaggacgtgaagagggaggtggccatcatgcggcgcatgtcgtcggcgtcgtcgtcgcgcgggggcggcgccgcgtcgtccgccgccgtggtgcggctccgcgaggcatgcgaggacgccgccgacggctccgtccacctcgtcatggagctctgcgagggcggcgagctgttcgaccgcatcgtggcgcgcggacactactccgagcgcgccgccgccaacatcttccgcaccatcgtcgacgtcgtccagctgtgccactcgaacggggtgatacaccgcgatctgaagccggagaacttcctgttcgcgaacaaatcggaggactcgccgctcaaggtcatcgacttcggcctctcggtgttcttcaagccaggcgaccggttcacggaggtggtggggagcgcgtactacatggcgccggaggtattgcggcggagctacgggccggaggtggacgtgtggagcgccggcgtcatcctctacatcctcctctgcggcgtccctccattctggggagacaacgacgagaagatcgcgcaggcgatcctccgcggcgccatcgacttcaacagggagccattgccgagggtctccgccaacgccaaggacctcgtcaggaggatgcttgatcctaacccatccacccgcctgacggccaaacaagttcttgagcatccatggctgaagaacgcggacacggcgccgaacgtgtcgctgggcgacgccgtgcgggcgaggctgcagcagttctccgccatgaacaagttcaagaagaaggctctcggagtggtggcgcggaacctgccgggggaggaggtggacaagtacgtgcagatgttccaccacatggacaaggacaagaacgggcacctgtcgctcgacgaactcttggaaggcctccacatcaacggccagcccgtccccgagcccgagatcaggatgctactcgaagccgcggacacggacgggaacgggacgctggactgcgacgagttcgtgacggtgtcggtgcacctgaagaagatgagcaacgacgagtacctggcggcggcgttcaactacttcgacaaggacggcagcgggttcatcgagctggacgagctgcgggaggaggtgggcccaaacgagcaggccatcctggagatcctccgcgacgtcgacaccgacaaggacggccgcatcagctaccaggagttcgagctcatgatgaagtccggcgccgactggaggaacgcctccaggcacttctccagggccaacttcagcaccctcagcaggaggctctgcaaggatactcttactccctga</cdnaseq>

Protein Sequence

<aaseq>MGGCYSAYASSRKLRGRISKISLVIPDPVPDAEAASPRKDGVDG DGDDVRGGGGGCDDGGDVVAIATTTADEFARRYVLGKELGRGEFGVTRRCSDAATGEA LACKTIRKHRRLAPPRVTAAKAAAAHGEDVKREVAIMRRMSSASSSRGGGAASSAAVV RLREACEDAADGSVHLVMELCEGGELFDRIVARGHYSERAAANIFRTIVDVVQLCHSN GVIHRDLKPENFLFANKSEDSPLKVIDFGLSVFFKPGDRFTEVVGSAYYMAPEVLRRS YGPEVDVWSAGVILYILLCGVPPFWGDNDEKIAQAILRGAIDFNREPLPRVSANAKDL VRRMLDPNPSTRLTAKQVLEHPWLKNADTAPNVSLGDAVRARLQQFSAMNKFKKKALG VVARNLPGEEVDKYVQMFHHMDKDKNGHLSLDELLEGLHINGQPVPEPEIRMLLEAAD TDGNGTLDCDEFVTVSVHLKKMSNDEYLAAAFNYFDKDGSGFIELDELREEVGPNEQA ILEILRDVDTDKDGRISYQEFELMMKSGADWRNASRHFSRANFSTLSRRLCKDTLTP</aaseq>

Gene Sequence

<dnaseqindica>3685..4038#2252..2419#2051..2166#1788..1940#1102..1243#882..1007#123..761#gcgagccgcgcgagcgcgacacacacagaggaggaggccgcgcgcgcgacgcgagctcgccctctgcgtgcgccgccgcgaggtcgccgccgccgccgccgccgtcgatcggcgcccgatcgatggggggctgctactccgcctacgcctcctcgcgcaagctgcgcggccgcatcagcaagatctccctcgtcatccccgacccggtccccgacgccgaggccgcctcgccgcgcaaggacggcgtcgatggcgacggcgacgacgtgaggggtggtggtggtggctgtgacgatggcggtgacgtcgtcgccatcgcgacgacgacggcggacgagttcgcgcggcggtacgtgctggggaaggagctggggcgcggggagttcggggtgacgcggcggtgcagcgacgcggcgaccggggaggcgctggcgtgcaagacgatccggaagcacaggcgcctggcgccgccgcgggtgaccgcggcgaaggcggcggccgcgcacggggaggacgtgaagagggaggtggccatcatgcggcgcatgtcgtcggcgtcgtcgtcgcgcgggggcggcgccgcgtcgtccgccgccgtggtgcggctccgcgaggcatgcgaggacgccgccgacggctccgtccacctcgtcatggagctctgcgagggcggcgagctgttcgaccgcatcgtggcgcgcggacactactccgagcgcgccgccgccaacatcttccgcaccatcgtcgacgtcgtccaggtaataatcaagaacgaacactacacatcatcttcttcttctcccattgatgcatcaaatcaaacgagtaatcactaattaatcaccaacaatctgtgatcgatcggtcgatggatgcagctgtgccactcgaacggggtgatacaccgcgatctgaagccggagaacttcctgttcgcgaacaaatcggaggactcgccgctcaaggtcatcgacttcggcctctcggtgttcttcaagccaggcacgtcgatcttcagcaaaatttccacctctcatcgtccatgatttctgtcaaatcctccatggtttctgaaaccaatcaaatgttgatgcaggcgaccggttcacggaggtggtggggagcgcgtactacatggcgccggaggtattgcggcggagctacgggccggaggtggacgtgtggagcgccggcgtcatcctctacatcctcctctgcggcgtccctccattctggggaggtatatactcaccttaattacctttttttttaatatacaatgtcattgatttttaaagatatgtttgataatttttttgaaaaaaagattatgtgattaccgcttattttattgtgagtttattgttaattattctataagtatgacttaaacttttttttatagtttgcaaaagttttgaataatacgaatagtcaaatatttataaaaaaaataacgggatcatatcttaacttccttttttactatatatatgatactatttaactttttagtcatgttcaatcattttaccttataaaaatattaaatatttttcgtttgatttatcactaagaaactttaaacattatttatgttttaaagatttacatattttttttaataaaacgaattgtcaaatattaaaaaaaaagttaacggtatcatttattaaaaaaatacattaccaattcaccatgatgatgcaaatcatctcccctgtcaattcttgtcatgaaaaaaaactataactgtaatcttgtctgaacttgcaaatgtgcagacaacgacgagaagatcgcgcaggcgatcctccgcggcgccatcgacttcaacagggagccattgccgagggtctccgccaacgccaaggacctcgtcaggaggatgcttgatcctaacccatccacccgcctgacggccaaacaagttcttggtaagtaaacaacttaatcaaaccttctcaatgattcagatatacacatgattactggaaatcaaacatcattttgtttgtttcgtcgattggttttgaaacaaaaacagagcatccatggctgaagaacgcggacacggcgccgaacgtgtcgctgggcgacgccgtgcgggcgaggctgcagcagttctccgccatgaacaagttcaagaagaaggctctcggagtaagtcgtcgagttgtctcattgcttctgtgttgcgatgatcttgatgggtcgccggagctaacaacggcggtgtccggtgaaggtggtggcgcggaacctgccgggggaggaggtggacaagtacgtgcagatgttccaccacatggacaaggacaagaacgggcacctgtcgctcgacgaactcttggaaggcctccacatcaacggccagcccgtccccgagcccgagatcaggatgctactcgaagccgtaagctttctgctcatctcctcgatctcgatctcagaatcattttctttattaagataatggaagatttttatcgagctcattcaattacatggagaagatctcaaaaatcatttttcttgagtcttgatctcagaatcattttcttttaagataatggaagatttttatcgagctcattcaattacaccgaggagatctcaaaatcattatttttctgaagattttgttgagatcatccaattatatcgagaagatattactgtaaaccgttgaattgtgatggatgatcatactctcattttctcgatctcaaaatcgttttttaaaaaagataataaaatattttatcgagctcatccaattacatcgtgaagatataactgtaccgttgaattggggtggtcagaccctcgtattctcactctcaaaatcgcttttcaaagataatgcaagatttttatcgattgaacttattagctaatttattgattacttgtcgttttagacaagtaaacggtcttaaataaacaattatagccaccattttctattacaatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatagtaaatttgtttggtgctccatggtgcccgggcaccattgttgaaattgagtatatacctaattcaaatgagtatgaaactttttcaattacttagatattaacattagctactttactaactagttcaaagcaagtttgaactgaatttgaacttgtttttgttagattttgattctaggtatatagcatccatacatataattagttaggtatgtaatcatggattgtgaaataaagttaaatttgagttgttttgttgataggtataggtatgaatcgagttattataactaggtatatactcacaatttgaaaattttgaaaaatttgagtgattttgtgtatttgatatcatggtgccccatatgagtgtcctatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatattagaaaaaatattttattgtagtatttttaagactaatttctacatatggtctctacatttctacgatttaagaaagtttgaccaaattattatccaaaacaataaatatcatcaactcggaggtattacatcgaggatatagtaagaagatgagatgatgaatttgcaggcggacacggacgggaacgggacgctggactgcgacgagttcgtgacggtgtcggtgcacctgaagaagatgagcaacgacgagtacctggcggcggcgttcaactacttcgacaaggacggcagcgggttcatcgagctggacgagctgcgggaggaggtgggcccaaacgagcaggccatcctggagatcctccgcgacgtcgacaccgacaaggacggccgcatcagctaccaggagttcgagctcatgatgaagtccggcgccgactggaggaacgcctccaggcacttctccagggccaacttcagcaccctcagcaggaggctctgcaaggatactcttactccctgatgatcgatgatgcccaacaatctcatcaactttgtttcatcaaggcttataaactgcacatctttcaccttcaccagacagggataactagagagagatacaagaccaacttagagaaatgtattggagtggcaacacgatgcgatgctgcgcttggattgtgaaatgagaaatggaactgaaatggggatcgtaccggttttgct</dnaseqindica>

External Link(s)

NCBI Gene:Os08g0540400, RefSeq:Os08g0540400

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