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| − | {{JaponicaGene|
| + | '''''OsGL1-2''''' is a member of the '''GL1-like genes''' in rice<ref name="ref1"/>. |
| − | GeneName = Os02g0178800|
| + | |
| − | Description = Similar to Glossy1 protein|
| + | ==Annotated Information== |
| − | Version = NM_001052615.1 GI:115444600 GeneID:4328496|
| + | ===Function=== |
| − | Length = 7316 bp|
| + | *The full-length cDNA of ''OsGL1-2'' was isolated from Minghui 63 by gene-specific primers and '''cloned''' into pCAMBIA1301S under the control of Cauliflower mosaic virus 35S promoter<ref name="ref1"/>. |
| − | Definition = Oryza sativa Japonica Group Os02g0178800, complete gene.|
| + | |
| − | Source = Oryza sativa Japonica Group
| + | *''OsGL1-2'' gene has a significant role in '''controlling the wax layer''' and thus '''protecting leaves''' from '''water loss''' in rice<ref name="ref1"/>. |
| | + | |
| | + | |
| | + | '''GO assignment(s):''' [http://amigo.geneontology.org/amigo/term/GO:0003824 GO:0003824],[http://amigo.geneontology.org/amigo/term/GO:0008152 GO:0008152] |
| | + | |
| | + | ===Mutation=== |
| | + | *Among '''23''' independent transgenic plants generated, '''nine''' transgenic plants showed high expression level of the transgene<ref name="ref1"/>. |
| | + | |
| | + | *Compared to the over-expression and wild type plants, the ''osgl1-2'' mutant was more '''sensitive''' to '''drought stress''' at '''reproductive stage''', suggesting an important role of this gene in '''drought resistance'''<ref name="ref1"/>. |
| | + | |
| | + | *The crystallization patterns of cuticular waxes on matured leaves of the osgl1-2 mutant were significantly different and reduced compared to OsGL1-2 overexpression and WT plants<ref name="ref1"/>. |
| | + | |
| | + | *The ''osgl1-2'' mutant showed rapid water loss and decreased epidermal permeability compared to the ''OsGL1-2'' over-expression and WT plants, suggesting that the mutant plants may be susceptible to drought<ref name="ref1"/>. |
| | + | |
| | + | ===Expression=== |
| | + | *Compared to the wild type, the ''OsGL1-2''-over-expression rice '''exhibited more wax crystallization''' and '''a thicker epicuticular layer'''; while the mutant of this gene showed '''less''' wax crystallization and a '''thinner''' cuticular layer<ref name="ref1"/>. |
| | + | |
| | + | *The expression levels of ''OsGL1-1'' and ''OsGL1-2'' were gradually '''increased''' throughout the '''time course''', whereas the ''OsGL1-6'' showed strong induction only at 2 day after treatment<ref name="ref1"/>. |
| | + | |
| | + | *''OsGL1-2'' was '''induced''' by '''ABA treatment''', and the highest induction was at 12 h after the treatment. RT-PCR analysis showed that the transcript of ''OsGL1-2'' gene was '''broken down''' in the 04Z11ML82 mutant<ref name="ref1"/>. |
| | + | |
| | + | *The ''OsGL1-2''-overexpresion plants showed a significant '''decrease''' of chlorophyll leaching and the ''osgl1-2'' mutant plants showed a significant increase of chlorophyll leaching, which indicates that over-expression of ''OsGL1-2'' genes '''decreased the cuticular permeability''' and '''perhaps''' can cause '''drought tolerance''' of the plants<ref name="ref1"/>. |
| | + | |
| | + | ===Evolution=== |
| | + | '''GL1''' shows high sequence similarity with OsGL1-1(76%), OsGL1-2 ('''62%'''), and OsGL1-3 (62%), suggesting that the OsGL1-1~3 may be '''homologs of GL1'''<ref name="ref1"/>. |
| | + | |
| | + | ===Knowledge Extension=== |
| | + | *The outermost surfaces of plants are covered with an epicuticular wax layer that provides a primary waterproof barrier and protection against different environmental stresses. '''Glossy 1''' (GL1) is one of the reported genes '''controlling wax synthesis'''<ref name="ref1"/>. |
| | + | |
| | + | ==Labs working on this gene== |
| | + | *National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, 430070 Wuhan, China |
| | + | *Department of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi 6205, Bangladesh |
| | + | |
| | + | ==References== |
| | + | <references> |
| | + | * <ref name="ref1"> |
| | + | Islam M A, Du H, Ning J, et al. Characterization of Glossy1-homologous genes in rice involved in leaf wax accumulation and drought resistance[J]. Plant molecular biology, 2009, 70(4): 443-456. |
| | + | </ref> |
| | + | </references> |
| | + | |
| | + | ==Structured Information== |
| | | | |
| − | ORGANISM Oryza sativa Japonica Group
| |
| − | Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
| |
| − | Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
| |
| − | clade; Ehrhartoideae; Oryzeae; Oryza.
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| − | |
| |
| − | Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|
| |
| − | AP = Chromosome 2:4355070..4362385|
| |
| − | CDS = 4355207..4355362,4355574..4355747,4356234..4356344,4356506..4356712,4357609..4357716<br>,4358442..4358698,4359120..4359241,4359782..4360001,4360877..4361130<br>,4361782..4362005,4362136..4362189|
| |
| − | GCID = <gbrowseImage1>
| |
| − | name=NC_008395:4355070..4362385
| |
| − | source=RiceChromosome02
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| − | preset=GeneLocation
| |
| − | </gbrowseImage1>|
| |
| − | GSID = <gbrowseImage2>
| |
| − | name=NC_008395:4355070..4362385
| |
| − | source=RiceChromosome02
| |
| − | preset=GeneLocation
| |
| − | </gbrowseImage2>|
| |
| − | CDNA = <cdnaseq>atggctgctcctcctctctcgtcgtggccatgggcaagcctcggctcgtacaagtatgtgttgtacggggcggtggtgtggaaggtggcggaggagtggaggcagcaaggggcggcgccggtggggtcgtggtggctccacctgctgctgctgttcgccgcccgcgggctcacctaccagttctggttctcctacggcaacatgctcttcttcacccgccgccgccgcgtcgtccccgactccgtcgacttccgccaggtcgacgccgaatgggactgggataactttctgctgctgcagacgctgataggggcaacgttggtggggagcccggcggtggcgcggcagcagctgctgctcccaagcctgaagcaagcgtgggacccacgcgggtgggccatcgcactgctgctgcatgtgttggtcgctgagccgctcttttactgggcccaccgggccctgcaccgggccccactcttcagccgctaccacgccgctcaccaccacgcctcggtcaccacacccttgacagctggatttgggacgccactggagagcctgctgctgacggtggtgataggggtcccactcgcaggggcctttctgatgggggtagggtccgtgggcctggtctatggacacgtccttttgtttgacttcttgaggtccatgggctacagtaacgtggaggtgatatcccctagggtcttccaggctgttcctcttctcagatacctcatctacacccctacgtacctgagcctgcaccacagggagaaggactcaaacttctgcctgttcatgcctatttttgatctcctggggggcaccctcaaccacaagtcatgggagctgcaaaaggaggtctacttaggcaagaacgatcaggcgccggacttcgtgttcctggcacacgtggtggacatcatggcgtcgatgcacgtgccgttcgtgctccggtcgtgcagctcgacgccgttcgccaaccacttcgtcctcctcccgttttggccggtcgccttcggcttcatgctgctcatgtggtgctgctccaagaccttcctcgtcagctcctaccgcctccgcggcaacctccaccagatgtggactgtcccccgatatgggtttcagtacttcataccggcggcgaagaaaggcatcaacgagcagatcgagctggccatcctgcgtgcagatcggatgggcgtcaaggtgctcagcctcgccgctctcaataagaacgaggcactgaatggaggtggcacgctgttcgtgaacaagcacccggagctgagggtgagggtggtgcacggcaacaccctgacggcggccgtgatcctcaacgagatccccagcaacgtcaaggacgtcttcctcaccggcgccacctccaagctcggcagagccatcgccctctacctctgcagaaaaaagatcagagtcctgatgttgacattgtcgtcggagaggtttctgaagattcagagggaagcaccggcagagttccagcagtacctcgtgcaggtcaccaagtaccagcctgcacaaaactgcaagacgtggctggtggggaagtggctgtcgccgagggagcagaggtgggcgccggcggggacacacttccaccagttcgtggtgccgccgatcatcgggttccggcgagactgcacctacggcaagctcgccgccatgaggctccccaaggacgtccagggcctcggctactgcgagtacacgatggaacgcggggtggtgcacgcgtgccacgccggcggcgtggtgcacttcctggaagggtgggagcaccacgaggtgggcgccatcgacgtcgaccggatcgacgtcgtctggaaggcggcgctcaagcacggcctcacgccggcgtga</cdnaseq>|
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| − | AA = <aaseq>MAAPPLSSWPWASLGSYKYVLYGAVVWKVAEEWRQQGAAPVGSW WLHLLLLFAARGLTYQFWFSYGNMLFFTRRRRVVPDSVDFRQVDAEWDWDNFLLLQTL IGATLVGSPAVARQQLLLPSLKQAWDPRGWAIALLLHVLVAEPLFYWAHRALHRAPLF SRYHAAHHHASVTTPLTAGFGTPLESLLLTVVIGVPLAGAFLMGVGSVGLVYGHVLLF DFLRSMGYSNVEVISPRVFQAVPLLRYLIYTPTYLSLHHREKDSNFCLFMPIFDLLGG TLNHKSWELQKEVYLGKNDQAPDFVFLAHVVDIMASMHVPFVLRSCSSTPFANHFVLL PFWPVAFGFMLLMWCCSKTFLVSSYRLRGNLHQMWTVPRYGFQYFIPAAKKGINEQIE LAILRADRMGVKVLSLAALNKNEALNGGGTLFVNKHPELRVRVVHGNTLTAAVILNEI PSNVKDVFLTGATSKLGRAIALYLCRKKIRVLMLTLSSERFLKIQREAPAEFQQYLVQ VTKYQPAQNCKTWLVGKWLSPREQRWAPAGTHFHQFVVPPIIGFRRDCTYGKLAAMRL PKDVQGLGYCEYTMERGVVHACHAGGVVHFLEGWEHHEVGAIDVDRIDVVWKAALKHG LTPA</aaseq>|
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| − | DNA = <dnaseqindica>7024..7179#6639..6812#6042..6152#5674..5880#4670..4777#3688..3944#3145..3266#2385..2604#1256..1509#381..604#197..250#ggatctcttcttataagtagagagggagacgcagcgtgtggcatccaaagctctctctctctctctctcatagctagctagagcatgcagctactagctagcaagcttaattagcagccacacattagagtcacaaagtgagagagaaggaggagcttcacaagctcgatcgagagagcaaactaagcaagcagccatggctgctcctcctctctcgtcgtggccatgggcaagcctcggctcgtacaaggcatgcatataatctctctcttaagtcgtcaattaatctctctttgttagtttcttcttcttcttcttcttcttctaccttattaagctaattaacgtgtgcgtggttaattaatgggagatgggtgcagtatgtgttgtacggggcggtggtgtggaaggtggcggaggagtggaggcagcaaggggcggcgccggtggggtcgtggtggctccacctgctgctgctgttcgccgcccgcgggctcacctaccagttctggttctcctacggcaacatgctcttcttcacccgccgccgccgcgtcgtccccgactccgtcgacttccgccaggtcgacgccgaatgggactggtacgcccttcgccgtctctctctctatctatctcattctcctccgccgtagctagctagtcgccggccggtcgccgacggcgtgcatttgtgtcctattcatttttagattgatttttttgagagggagaaaatatggctttgcatccaaggcaagcagtggtcaccaagcccagagacagtactgtaccatccagtcatccactgttagatcagatcccagcttcagccttcagaagagttcagacagacagacgccatggctgctctcttatctagaatcatcgtaattactactcatatatgtcaccatttctactgcttaaaccagcaccagcagtaaaattaagaatgaatctgttttatgtagacctccctccctcaatcctaaaataactataagtatagttttatactgtccttatctaacgtttgaccgttcgtcttatttaattttttaattatttttatttattagatgataaaacatgaatattactttatatgtgactaattattttataaaatttttaaataatacgaacggttaaatgttagaaacggatatccacggcaacacttatttttagaatggaggtagcaatatgtgtttttctttttttgatgggattattttgcatatatatgcagggataactttctgctgctgcagacgctgataggggcaacgttggtggggagcccggcggtggcgcggcagcagctgctgctcccaagcctgaagcaagcgtgggacccacgcgggtgggccatcgcactgctgctgcatgtgttggtcgctgagccgctcttttactgggcccaccgggccctgcaccgggccccactcttcagccgctaccacgccgctcaccaccacgcctcggtcaccacacccttgacaggtactctacacctgtactcatcatgtactgtactccataccatactccccaacaaagtttaggagtataaaaagggagtgtatttattcctatctatgaactgtttggtgtaccaaatttgttttgaaaattctcgttggaactgtgaaccagtttatgtatgatgttttgttgtcttaatgagttgcagcagatattttgaaacttaatgttggttttgggatagattttgaagtgttttatttgtcgtagtttgttttttctagcattgacttttaaacaataaacaataaaaatataaaatgttatgcatagattattcttttttctgcttcattcatactccctccgtattttaatgtgtgacgccgttgacttttcgactaacgtttgaccattcattttattcaaaattttcgtgcaaatatgaaaatatttatatcatgcttaaataacatttgatgatgaatctagtcacaataaaataaatgacaattgcataaattttttttaataagacgaatggtcaaacgttggataaaaagtcaactgcgtcatacattaaaatatggagctagtatttctacattgattaacccatcagagtggtacaatttatgaagattaaattaatcgagactagcctgaaattttaattttaaaacgtaattatgaagttgattttaagtttttttaatcgtgatccatattttagcaagatttttttatatatattttagcattgtagatataaatattttatctataatttatttttaattatacgtctcatacggttatacggttatagcgttgtaggagtacaattttgactggctgatgataagcttttcctgtgttacgttggtctgcagctggatttgggacgccactggagagcctgctgctgacggtggtgataggggtcccactcgcaggggcctttctgatgggggtagggtccgtgggcctggtctatggacacgtccttttgtttgacttcttgaggtccatgggctacagtaacgtggaggtgatatcccctagggtcttccaggctgttcctcttctcagatacctcatctacacccctacgtgagtgcaaaaataaaataatagtataatgtatttgttttctgataaatacttctctattcataacttttggtaaattggattgaaaattattttagttgaccaccgatatcttgtaaatcgtttgattcaattaatgggaactatatgcttatattttttaatagctaccagtttatttttaaaataggagtgatcggactaaaacaatgcaaggtcaaaattatcaagtattgtaaaatggagaaaataagtaaattatttttgtctaagacagttagttaaataaataaataactactttataaggttgagatacaagtagtactaattatgctgcacttcattgtatggaccgtgctgctatctgcttaattgcatctgtcatttggtcactatcctagtgttgttagctgtactgctaattggttgttagagaaaagttgattaattagtcggtgttgtatgaagaggtgatacaagtaattaaaatcgatttgattgttctaatgcattatgcatatggatttaattgcgcaggtacctgagcctgcaccacagggagaaggactcaaacttctgcctgttcatgcctatttttgatctcctggggggcaccctcaaccacaagtcatgggagctgcaaaaggaggtctacttaggtagggtgcacgcctaattagtgcattttattatgccttttgcttcaagaaacaagaattatattcagtcagtgtgctacaagacccataagaaacattaacatttggaattgattcctgcaaaattcccaagccccaaaaatcttgaagattttttacaattaaaacaaagagatttggagggagagacattgctccagacattaacatttagaattaattcatgcaaaattcccaagccccaaaaatcttgaatatttttacaataaaaaaacttttttaaagaagaaattatgagcgtgcggggattgaacacatgacctcaagggttgaaatcacacatctattatcatttcactatcgagagcttgacggttttataataatattatttcgcgaaaaatggcaattgttgatgtaggcaagaacgatcaggcgccggacttcgtgttcctggcacacgtggtggacatcatggcgtcgatgcacgtgccgttcgtgctccggtcgtgcagctcgacgccgttcgccaaccacttcgtcctcctcccgttttggccggtcgccttcggcttcatgctgctcatgtggtgctgctccaagaccttcctcgtcagctcctaccgcctccgcggcaacctccaccagatgtggactgtcccccgatatgggtttcaggtataatcacaataacccccccccccccccctctctcttctcgcctagcttaattccagaataatttaaaattatatcatatctcatgtcggtataacatgagacaatattttagaattagtatgtgatctcaccgtaaatttttcaccccgtcacatcgaacgtctaaagacatgcatgaagtattaaatatatgcttaaaaaataactaattgcataaattgcgactaatttgcaagacagatcatttaagcctaattgctccatgatttgataatgtggtgctacagtaaatatgtgctaatgacagattaattaggcttaataaatttgtctcgtagtttactgacggattttgtaattattttttttattagtgcccgaacaccccatacgacacactatataatacccgatgtgacacaccaaaactttacacccatgaatttaaacactctcgtactgactgtatcgagaggacaactgagatatcatggatggatcataggttgtccatcaattaattggtagaggtgttgtggtaccatactattcaatggcacctctctcgcaagtacttgtacagtacagtagtgacaacagtggttaatgtgtggctttaactttgcagtccctatagaaaactgcatgtgattcggttttgtgtaggccatctgaacttgtgaattgattctgacattggcgccatgtatgcaaattcacagtacttcataccggcggcgaagaaaggcatcaacgagcagatcgagctggccatcctgcgtgcagatcggatgggcgtcaaggtgctcagcctcgccgctctcaataaggtcagttgttttacaaaagatctggttcgtttcagctttttttttcgattattatcacgaattatttatttatttatttattcatgtcaccaagaatacattttttaaatatttatttattaaaacatttaatttatgtatataatcatatagataatccgttacaataatagtccaataatttatgtatatttaatatttgctacctgtacatgtcttgtctcttccatgcctcaacttttttttccttttcctttatcactttcttgcaatgacacagacacgtacatgtctctttctttgatcataatgtgccgtgttatgcatatagttgagcagtattggatatacagcatgtataataagatacttgtccattaactagtacagtatcactgctgatgattcagtaggggtgttctgtttttcctcatctttcttcaactttctccctgtcattttcaaaagaaaaaaaaggaaaaaaaagagagtatgtgatgttcatgtcagtctctttgctgttcatgtgtcgcccatacagttgggcagcattggttatctagcacagactaccggaaccaattcatcatcatttatgatgggcatgtgaaatccttaactgattcaacgttgaaatggcggcgaagatggaaatgcgaccgtgacgcgtgtaatcggagtagatgcagcggtggctagctcataactaaaaaatctgcgttcattgttcagacagttaaaaagttcaggaatttcatctgatcagcactgtaaacagaatctttaggctagcaatctagcattactcaaaaatcaaaatgcagtgcaacgatctgatcttaactgaaactctttgaatcttcttatgttttggtttggtttcttgtttctgtcagaacgaggcactgaatggaggtggcacgctgttcgtgaacaagcacccggagctgagggtgagggtggtgcacggcaacaccctgacggcggccgtgatcctcaacgagatccccagcaacgtcaaggacgtcttcctcaccggcgccacctccaagctcggcagagccatcgccctctacctctgcagaaaaaagatcagagtcctggttcgtgctctgctgcctgtctgaactctgaactgaaccccaaaaaaggagaaaaaaaactctgtgttaattcacagcatgattagatgagtatgttaaacaagaggatgattgcaagactgggattgatggttctgtgtgctaatcttttttggttttagatgttgacattgtcgtcggagaggtttctgaagattcagagggaagcaccggcagagttccagcagtacctcgtgcaggtcaccaagtaccagcctgcacaaaactgcaaggtaattcactcgctggaatttcaataagtttcagtaattcaaggctgtgtccatgatgtgattgtccacattttctttcatcaggttttatccaccaaccagttaacctataaaacatagacttttgtcagagtcatattgtcccgctgaccaattaacccagaaaaagttacattttttgtctcagttgtatgcaccgaccagatcaccaaaagtttactttagtgtttattttgggtctagctatatttatggcgccaagttgtatgcactagctagttcgacggaaaagcttagggtaattttaccatctaagaaggtacccgaaagttaccgtattttctgatgtaaaatttagtacttccgaatactaggtatctcaagatagtaaatttttttaatgtaagatttaagtatcccgggttactttttcaaataccgtaaaatttatcagaagctaaagttggatggatggcattgttgcagacgtggctggtggggaagtggctgtcgccgagggagcagaggtgggcgccggcggggacacacttccaccagttcgtggtgccgccgatcatcgggttccggcgagactgcacctacggcaagctcgccgccatgaggctccccaaggacgtccagggcctcggctactgcgaggtacttcctccgtttcatattacagtcactttaaacctttttttataacaaaactctttttagattcgactaaatttatagaaaaattttgtccattattattattattattattattattattattattattattattattattatattcttcttctccgagttcgctgacatggtcggccggcgatcgccgggaaacatatgtgtgcagtacacgatggaacgcggggtggtgcacgcgtgccacgccggcggcgtggtgcacttcctggaagggtgggagcaccacgaggtgggcgccatcgacgtcgaccggatcgacgtcgtctggaaggcggcgctcaagcacggcctcacgccggcgtgaccgccgccgccggaggccagcggcgaagccaacgagaacggccggcgatcttgtgggatttggtgtgctgtaagatagaagaaatgtacgagaaaaaaacgatttgccgtgggatttaaattaattaaaatttaatc</dnaseqindica>|
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| − | Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001052615.1 RefSeq:Os02g0178800]|
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| − | }}
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| | [[Category:Genes]] | | [[Category:Genes]] |
| | [[Category:Japonica mRNA]] | | [[Category:Japonica mRNA]] |
