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| Line 52: |
Line 52: |
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| | ==Structured Information== | | ==Structured Information== |
| − | {{JaponicaGene|
| |
| − | GeneName = Os03g0643300|
| |
| − | Description = Similar to AER123Wp|
| |
| − | Version = NM_001057288.1 GI:115454304 GeneID:4333554|
| |
| − | Length = 7948 bp|
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| − | Definition = Oryza sativa Japonica Group Os03g0643300, complete gene.|
| |
| − | Source = Oryza sativa Japonica Group
| |
| | | | |
| − | ORGANISM Oryza sativa Japonica Group
| |
| − | Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
| |
| − | Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
| |
| − | clade; Ehrhartoideae; Oryzeae; Oryza.
| |
| − | |
| |
| − | Chromosome = [[:category:Japonica Chromosome 3|Chromosome 3]]|
| |
| − | AP = Chromosome 3:25570392..25578339|
| |
| − | CDS = 25570634..25570775,25574557..25574790,25574907..25574987,25575198..25575295,25575429..25575581<br>,25576096..25576151,25576235..25576394,25576807..25577037,25577186..25577294<br>,25578033..25578190|
| |
| − | GCID = <gbrowseImage1>
| |
| − | name=NC_008396:25570392..25578339
| |
| − | source=RiceChromosome03
| |
| − | preset=GeneLocation
| |
| − | </gbrowseImage1>|
| |
| − | GSID = <gbrowseImage2>
| |
| − | name=NC_008396:25570392..25578339
| |
| − | source=RiceChromosome03
| |
| − | preset=GeneLocation
| |
| − | </gbrowseImage2>|
| |
| − | CDNA = <cdnaseq>atggcggcggcgctggcgaggcgtggcggtggggggctggcgcgcgccctggcgcgggggagggggatgtgctcggccacggcggcggagcgcgccgccggggcggcgctgacgtccgaggagctcatgcggatggagcgcgagcgcagcgcgcacaactaccatccaattccggtggtgttctccaagggagaaggttcacatatattggatcctgaaggcaacaaatacattgatttcctgtctgcttattctgcagtcaatcagggccattgccatccaaaagtcctgagagcgttgaaagagcaggcagaaaggctcacactgagttctagagctttctacaatgacaaattcccaatctttgcagaatacctgacaagcatgtttgggtatgaaatgatgttgccgatgaatactggagctgaaggagtggaaacagctatcaaattggtgaggaaatggggttatgagaagaaaaagataccaaaaaatgaggctttgattgtctcttgctgtggatgttttcatggtcggacattaggtgtcatctctatgagttgtgataatgatgcaactcgtggttttggcccattggttcctggccatcttaaagttgattttggagacactgatgggttggagaaaatctttaaagatcatggcgagaggatatgtggttttttgtttgaaccaatccaaggagaagctggggtaataatcccaccagatggctatttgaaagctgtcagagatttgtgttcaaggcacaacattctgatgattgcagatgagatccaaacaggcatagctagaactggcaaaatgctggcatgcgattgggaaaacatacgacctgatgtggtgattctaggcaaggcccttggtgctggagtagttcctgtcagtgcggttcttgcagataaggatattatgctgtgtatcaaaccaggagaacatggaagtacatttggtgggaacccattggcaagtgctgtggcagttgcatcactgaaagtagttacagatgaaggtctcgttgaaagggctgcaaagttaggacaagagttcagagatcagctacagaaggttcaacagagattcccgcaaatcataagggaagtacgtgggaggggtttgcttaatgcagtggatctaagcaacgaagctttatcccctgcttctgcttacgacatctgcatcaagctgaaggagagaggcgttctggcaaagcccacacatgacaccataatcagattagcgcctccgctgtcaatcagtcctgaggagcttgcagaagcatcgaaggcgttcagcgatgtgcttgagcatgacctgccacagctgcagaagcagatcaagaagacagaatccgcggcagaaaaacaatcctgtgacagatgcggcagagacttgtactga</cdnaseq>|
| |
| − | AA = <aaseq>MAAALARRGGGGLARALARGRGMCSATAAERAAGAALTSEELMR MERERSAHNYHPIPVVFSKGEGSHILDPEGNKYIDFLSAYSAVNQGHCHPKVLRALKE QAERLTLSSRAFYNDKFPIFAEYLTSMFGYEMMLPMNTGAEGVETAIKLVRKWGYEKK KIPKNEALIVSCCGCFHGRTLGVISMSCDNDATRGFGPLVPGHLKVDFGDTDGLEKIF KDHGERICGFLFEPIQGEAGVIIPPDGYLKAVRDLCSRHNILMIADEIQTGIARTGKM LACDWENIRPDVVILGKALGAGVVPVSAVLADKDIMLCIKPGEHGSTFGGNPLASAVA VASLKVVTDEGLVERAAKLGQEFRDQLQKVQQRFPQIIREVRGRGLLNAVDLSNEALS PASAYDICIKLKERGVLAKPTHDTIIRLAPPLSISPEELAEASKAFSDVLEHDLPQLQ KQIKKTESAAEKQSCDRCGRDLY</aaseq>|
| |
| − | DNA = <dnaseqindica>7565..7706#3550..3783#3353..3433#3045..3142#2759..2911#2189..2244#1946..2105#1303..1533#1046..1154#150..307#gcgagctcgcgacgcgaaccccctacgccgcagccgcaggcttcctcttcatctcctcctcctccagacctccacgcgagcgtgccccggggtggaagcggaggcggcggcggctcgagcgcaatctggtggcgtgggggcgcgtcacgatggcggcggcgctggcgaggcgtggcggtggggggctggcgcgcgccctggcgcgggggagggggatgtgctcggccacggcggcggagcgcgccgccggggcggcgctgacgtccgaggagctcatgcggatggagcgcgagcgcagcgcgcacaagtacggtttgcccctcgatctccccctccccttttccttccttcctcgctctcttgtgctgcgatctctctcaccgtgagtgcctgcctgaatgctcccccctcctccacggttcagtccccgactcgctggtccggcggatcgtactcggatcgcgtttcagactgagaattccgcgctcgtgatcgatttttttcttgctcaggaattccccctttcttcggttagtcctctgtttatcttgcatagtgccgtgggagagggagatcgtatgattacttcctcgaattggggatgtagaagtagtgtacaactgatacttgtacgcgtgggagagggagactgggctcaactcactctcgaatgttgtttatatcaaggactaggccacttctggatactgaccgagtgttgtttaatgtttatcactaagtaccatgtcagcgacattgatggtgactctgatatgtcccagggagttagtactgctgttgcatttggttgcgataaagacgtctctactacttgccttgtctcattggctggaaattctgaggttctagttaataagcatagagcaattgaacctctggttttatttttcacaaattatgcaacaagttatatgaagcttatgcatatgtctgggacaaaaggaactttcacctgtcactgcaagccttttattttgatattctagttgcttcctaacagagcttgaacattcacttcctgcagctaccatccaattccggtggtgttctccaagggagaaggttcacatatattggatcctgaaggcaacaaatacattgatttcctgtctgcttattctgcagtcaatcaggtgattttttttcctggtttgttacatcttgtaattgaatgtcttttggtcaaaaagcacatattagaaggcaaacatgttggtatccattgtttcagccagcttatatcatttttcataattgatgtagtttttgcaattgttgtagggccattgccatccaaaagtcctgagagcgttgaaagagcaggcagaaaggctcacactgagttctagagctttctacaatgacaaattcccaatctttgcagaatacctgacaagcatgtttgggtatgaaatgatgttgccgatgaatactggagctgaaggagtggaaacagctatcaaattggtgaggaaatggggttatgagaagaaaaagataccaaaaaatgaggtatgatttcccaaaagctacatgtttcatttcaacctggcaatatttctctccatctggccctttgtgttgccatgaaggtgcagaaaaatccgagtaagctttgactagatgatatccatattccttgctttgcataatgctgctgctcttaatattttgctcattagttggatatcgttaaagatatcattatctttatgtaaatggaagcagaattaaatgaaatagcaaaacaattcacaagagttgcctactgtttttccttactcttaactgtttgattgctggtgtacaatataactatataatatctatgatagtccatattttggcaaatgtatcacttaacggctttgaaattagagattcattggtacctgttttttgtgaaatgttcggtcgtctgcaggctttgattgtctcttgctgtggatgttttcatggtcggacattaggtgtcatctctatgagttgtgataatgatgcaactcgtggttttggcccattggttcctggccatcttaaagttgattttggagacactgatgggttggagaaaatctttaaaggttagctcatatgtttatttgtagcaaccatattctacactatttttgagtatttgcatgttcatgaacgctatttgattcagatcatggcgagaggatatgtggttttttgtttgaaccaatccaaggagaagctggggtatgtattttttaagttactctgtatttatttttcaaaaagttctagatcgaattcttgctagttttgtattgtgttgtatagtgatatactgattctgatatactactgtataaagttataaatctctaatacaatttaaaaaaaaaatagttgactggaagactttccaaaccctgcatgatagtcaatttcttgacttcacatctaagggtattactaacaaatggtatacatggtttgacacctgttccagcctaatttccatgatatgtttttaagccatttacagatactcaggtaatttgattaattcaatttagaaacatggaactagagattggtcctaaagcacttttcaagttaaggtggtaaactagaatggggctatattgataactaaagcatgtgctgtatactctagccattgtgctacttgtatccatattttttgttattctcatcagcttgtgatatcctcgtgttaaaaattccacaaacctactgctcacaggtaataatcccaccagatggctatttgaaagctgtcagagatttgtgttcaaggcacaacattctgatgattgcagatgagatccaaacaggcatagctagaactggcaaaatgctggcatgcgattgggaaaacatacgacctgatgtggtggtaagtttctagtttctgctacactgtttaacctcttcttactgcacaacgacactagtatctatccgctgggagtttttatttgctggagaatattcagctacaatgttgatcaagtatctccactgttcagattctaggcaaggcccttggtgctggagtagttcctgtcagtgcggttcttgcagataaggatattatgctgtgtatcaaaccaggagaacatggaaggtatgggccattttcaatccttctcttttggttcactccaacaaggagttaaattgtgaactaattcagttctttaatcaagcattaatgtctgtgcaatgccctagtttttcttttccagtgtataacatttttgctacttttagataagcaagttttttgcttccccccccctctttcttgatcagtcttatgtttgatccctgtcagtacatttggtgggaacccattggcaagtgctgtggcagttgcatcactgaaagtagttacagatgaaggtctcgttgaaaggtacattgttttgttgcaagagaaatattgttatttcctataacatcagctgtttcatgattcagctgtatgattgtttatgagtatctttcttctaaacatttactgaactacagggctgcaaagttaggacaagagttcagagatcagctacagaaggttcaacagagattcccgcaaatcataagggaagtacgtgggaggggtttgcttaatgcagtggatctaagcaacgaagctttatcccctgcttctgcttacgacatctgcatcaagctgaaggagagaggcgttctggcaaagcccacacatgacaccataatcagattagcgcctccgctgtcaatcaggtatgtcctttgctgttatgtctttatggttgtgttagtatcatttttttagataacgggcagcgcccggcctctgcatcataaggtgaatgcacacggccaaacaaagtacaaataggtacatagaccccggagttttaacaaaatgatggcacagccaacctaaggattacatccaaataagcaagtttcaaacacgcaagagaggaaaccaaagtctacgcttgtaatcttcctctaaaattccatccctgcttggaaaagaaatccatcacattggcttctagcaacatgcatcctttcttcaccatgattccatcttcttcattaagcaacaaggcccattgcctcgcccagtgtgttcctctgaatattacctgcataaaagaattaattttggtaccatgaaatacctcatcatttcggcataaccataacgcccaacatagcgccccaatacaccccagaaagtgacatcttagcttaggatgcaaaccattaagccaactaccgaaaagatgcgcaacactattaggcggtggaataccaaaggtaatatgtatagcactccaaatataccttacaatacgacattcaaggaaaaggtgatgtatgttctcgttagagctacaaaaacaacacttagtgctacctttccactttcttttcgccatattatcttttgttaacataacgcccttttgcatgtaccacaagaaaatctttattttcagtggaattctaagtttccaaatcaaagatttccttggtataatatcttgatacataatcgccttgtacatagaatttaccgaaaaggttccatctcctttcaacctccacttgaacgagtcactttgttccgagaggttaaaagaacagacttttgccactaattggtaccaatatcttcggttatcccctatcaatgccctcctgaacgacacatttaaaggcagcgtgcttagcacccctgctacacttacattccttctcctaaccaacgaataaagtgtggggaaaactttgtcgaaagacgtatccccacaccaacagtcctcccaaaaccttacttgtgtcccatcatgaactacccaagatctgaaagaaaggaagtccctcttaacctccatgagtcctccccaaaagtgtgagtctcctggactcctttctacctgggttatagtcttatttgacaagtatttccttctaagcaaggtttgccacaccccttcctcgtttatcaattggaacaaccatttgcttagcaaacatctattctgtgtttccaagttttggattccgaggccaccacactccttagggctacataaaacactccatttggccaacctatacttcttcttattctcatcacactgccaaaagaacctggacctataatagtctagcttcttaagtatacccttaggtatctcaaaaaaagagagcatgaacatggccaaactactcaaaaccgaattaatcaagaccaacctccctcctaccgacaagtgtttccctttccagctacttagttttttctggaatctatcctcaatagcctgccaatccttgtttgagattctcttatgatgcattgggatacctagatatttaaatggatactttcccatatcacacccaaaattctttacgtaatccatcatcacttcatttgccttaccaaaacaaaaaagctcacttttatgaaagttgatcttaagcccagataatttctcaaaggtgcttagcactaacttgagattcctggcctcctctagatcatgctccataaagaggatagtatcatctgcgtactgcagaattgagagcccatcgtctacaagatatggaacgagtccacgaattttcttttgctcattcgccctgtgcattagcaatgctaacatatcagccactaggttaaagagtattggagacaacggatccccttgtctaagcccttcttttgtttgaaaaaagttttccatatcctcgttcaccttcacagctacactccctcctctcacaatcatatcaatccattcacaccactttggagagaagcctttcatcttcaaagtttgttgcagaaacctccaatcaactttatcatacgccttctcaaaatctagtttcaagataactccgtctcttcttttcctatggatctcatgaattgtttcatgtaaaattactaccccttccataatgtttctccctggcaagaaggctgtctgtgatggtctaataaccttttgagctactagagctactctattggctatgaccttagtaaaaatcttaaaactcacattcaaaagacaaattggtctatattgttgaatctttcttgcttcctcttgcttcggtaacagagtgatgatgccaaagtttaggctatgaaggggtaacttcatatcatgaaagtcatgaaacatagccatgagatcatccttaattacatgccagaacacttgatagaactctgccggaaaaccatctggtcccggcgccttattatgtccatttgaaagatggcctcttttacttcgtcatctgaaaaactttttgttaacacttcattttcttcttccgaaacctgaggaatgtcctctctttgcgattccatcaaggaaaagttatttcccactggagccccaaacaggtctttataaaatttggtgatatacttcttaatttgtacatcccctctgattactccttcctcctgttccagctgaaaaatcctagtcttcctatgctttccatttgctatcaaatggtagtatttcgtgtttcgatccccttctaagattcgctttgtttttgccctttgaagccacttaatttcctcttccctcagcaaaaaagatagcctctgtttcacataattttttaggtccaactcctgtctagacagtaattgagactccgccttcttatctagctcttccactttcgcgattagaatagccttttcctttttataagctccattaatgttcttagcccatcctctcaaaaactgtctcaacctcctaatcttattttgccatttctctaacttggtcgaccctctactttcctttctccaaacctctgtgaccaattctagaaaaccttctcttaacaaccaccctagctcaaacttaaaaagcggttgtttatttccttgagtccccgatccagtatccaacaaaagtggcgtgtgatcagagaactctctattcagtgctctaacagtagcaagtggaaatcttagttcccattctgtactggtaagcaccctatctaatttttcaaatgttggattttgcatagagcttgcccaagtgaacttgcgaccagatagttctaactctctcaagttaacactgtcaatgaccacattaaataaaaatggccacttgtcattgtacctatcattatttttctcactaggatttctaatgatattgaaatcccctcccaccaacaatggacacggttctttattacatgcattcaccaactccactaagaagtcctctttgaattcctcctgagcagctccatagactgccacaagaatccactgaaagccatcctccttatttctcacatgaaacttgacataaaactctccctcatcaattgaagccacctccataacctctaagttaattcctaagagaatcccatcagacctacctctcggtatgttcaataaagttttgttttgttttttttgttttgttttttttgcggtaatggctaaaaatcctcttaatttcagtcctgaggagcttgcagaagcatcgaaggcgttcagcgatgtgcttgagcatgacctgccacagctgcagaagcagatcaagaagacagaatccgcggcagaaaaacaatcctgtgacagatgcggcagagacttgtactgatgagtgatagacgagacaatgaaacgtttccagaggcacccgtttcgcccaaataaaatagcagaacacacctcgcgaattcatagctcattgtagtagtagagtaggatatatacacctccttgttgcgatgtctgggacatatggtccagggatttgtcgcctaatcgagctggttgcaaacaaatggggtagcattattctagtctatcatatcatgcagaagaatcttgaattggatg</dnaseqindica>|
| |
| − | Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001057288.1 RefSeq:Os03g0643300]|
| |
| − | }}
| |
| | [[Category:Genes]] | | [[Category:Genes]] |
| | [[Category:Japonica mRNA]] | | [[Category:Japonica mRNA]] |
An ornithine δ-aminotransferase gene OsOAT confers multi-stress tolerance in rice[1].
Annotated Information
Function
- OsOAT is a direct target of the stress-responsive NAC transcription factor SNAC2[2]. OsOAT is responsive to multiple stresses and phytohormone treatments mainly through enhancing ROS-scavenging capacity and Pro pre-accumulation. Both ABA-dependent and ABA-independent pathways contributed to the drought-induced expression of OsOAT[1].
- OsOAT encodes a putative ornithine δ-aminotransferase, was upregulated' in SNAC2-overexpressing plants[2]. SNAC2 could bind to the promoter fragment of OsOAT[1].
GO assignment(s): GO:0008483,GO:0030170
Mutation
Three independent transgenic lines[1]:
- OsOAT-OE-7
- OsOAT-OE-10
- OsOAT-OE-17
- The δ-OAT activity was significantly higher in the OsOAT overexpressing lines than in wild-type.
- Under normal moisture conditions, the OsOAT overexpressing plants accumulated significantly more Pro than wild-type.
- Under drought stress conditions, the free Pro content increased in both the transgenic and wild-type plants. However, the Pro content in the OsOAT-overexpressing lines was slightly lower than that in the wild-type under drought stress conditions.
- The glutathione (GSH) content and activity of reactive oxygen species (ROS)-scavenging enzymes, such as glutathione peroxidase, were also increased in OsOAT-overexpressing plants.
Expression
- Overexpression of the OsOAT gene in rice resulted in significantly enhanced drought and osmotic stress tolerance. Overexpression of OsOAT caused significantly increased δ-OAT activity and Pro accumulation under normal growth conditions. In addition, OsOAT overexpressing plants showed significantly increased tolerance to oxidative stress[1].
- The transcript of OsOAT was induced in leaves 3 d after the initiation of drought stress, and the induction increased on day 5 and was maintained on day 7 of the drought stress experiment. The OsOAT transcript was also induced by high-salinity, heat, and submergence. transcript of OsOAT was strongly induced by ABA and IAA, and it was slightly induced by BR and JA[1].
Evolution
OsOAT has only one splicing mode and contains a 1422 bp open reading frame, which is predicted to encode a 473 amino acid protein with a molecular mass of 51.45 kDa. Accordingly, a putative N-terminal transit peptide with a barely conserved sequence for mitochondrial targeting exists in plant and animal δ-OATs including OsOAT. However, such an N-terminal sequence is absent in the δ-OATs from fungal and bacterial species[1].
Knowledge Extension
- OAT is a mitochondrial enzyme containing pyridoxal-5′-phosphate as a cofactor, which catalyzes the conversion of L-ornithine to L-glutamate γ-semialdehyde using 2-oxoglutarate as a terminal amino group acceptor. It has been described in humans, animals, insects, plants and microorganisms[3].
- Based on the crystal structure of human OAT, both substrate binding and reaction mechanism of the enzyme are well understood[3].
- OAT shows a large structural and mechanistic similarity to other enzymes from the subgroup III of aminotransferases, which transfer an amino group from a carbon atom that does not carry a carboxyl function. In plants, the enzyme has been implicated in proline biosynthesis and accumulation (via pyrroline-5-carboxylate), which represents a way to regulate cellular osmolarity in response to osmotic stress. However, the exact metabolic pathway involving OAT remains a subject of controversy[3].
Labs working on this gene
- National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan 430070, China
References
- ↑ 1.0 1.1 1.2 1.3 1.4 1.5 1.6
You J, Hu H, Xiong L. An ornithine δ-aminotransferase gene OsOAT confers drought and oxidative stress tolerance in rice[J]. Plant Science, 2012, 197: 59-69.
- ↑ 2.0 2.1
Hu H, You J, Fang Y, et al. Characterization of transcription factor gene SNAC2 conferring cold and salt tolerance in rice[J]. Plant molecular biology, 2008, 67(1-2): 169-181.
- ↑ 3.0 3.1 3.2
Stránská J, Kopečný D, Tylichová M, et al. Ornithine δ-aminotransferase: an enzyme implicated in salt tolerance in higher plants[J]. Plant signaling & behavior, 2008, 3(11): 929-935.
Structured Information
