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| | ===Expression=== | | ===Expression=== |
| − | The SUI-family genes encoded base-exchange types of phosphatidylserine synthases (PSSs), which possessed enzymatic activity in a yeast complementary assay. Overexpression of SUI1 and SUI2 caused outgrowths of internodes during vegetative development, and we showed that expression patterns of Oryza Sativa Homeobox 15 (OSH15) and Histone4 were impaired. Furthermore, genome-wide gene expression analysis revealed that overexpression and RNA knockdown of SUI-family genes affected downstream gene expression related to phospholipid metabolic pathways. | + | The SUI-family genes encoded base-exchange types of phosphatidylserine synthases (PSSs), which possessed enzymatic activity in a yeast complementary assay. Overexpression of SUI1 and SUI2 caused outgrowths of internodes during vegetative development, and we showed that expression patterns of Oryza Sativa Homeobox 15 (OSH15) and Histone4 were impaired. Furthermore, genome-wide gene expression analysis revealed that overexpression and RNA knockdown of SUI-family genes affected downstream gene expression related to phospholipid metabolic pathways.Moreover, using Ultra-performance liquid chromatography–quadrupole time of flight-mass spectrometry, we analyzed PS contents in different genetic backgrounds of rice and showed that the quantity of very long chain fatty acids PS is affected by transgene of SUI-family genes. Our study reveals a new mechanism conveyed by the SUI1 pathway and provides evidence to link lipid metabolism with plant stem cell maintenance |
| | | | |
| | ===Evolution=== | | ===Evolution=== |
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| | ==Labs working on this gene== | | ==Labs working on this gene== |
| − | Please input related labs here.
| + | 1. National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Graduate School of the Chinese Academy of Sciences, Chinese Academy of Sciences, Shanghai, 200032, China. |
| | + | |
| | + | 2. Department of Cell Biology and Molecular Genetics, University of Maryland, College Park, Maryland, USA. |
| | | | |
| | ==References== | | ==References== |
| − | Please input cited references here.
| + | 1. Hengfu Yin;Peng Gao;Chengwu Liu;Jun Yang;Zhongchi Liu;Da Luo |
| | + | SUI-family genes encode phosphatidylserine synthases and regulate stem development in rice |
| | + | Planta, 2013, 237(1): 15-27 |
| | | | |
| | ==Structured Information== | | ==Structured Information== |
| − | {{JaponicaGene|
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| − | GeneName = Os05g0554400|
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| − | Description = Phosphatidyl serine synthase family protein|
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| − | Version = NM_001062804.1 GI:115465338 GeneID:4339554|
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| − | Length = 5432 bp|
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| − | Definition = Oryza sativa Japonica Group Os05g0554400, complete gene.|
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| − | Source = Oryza sativa Japonica Group
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| | | | |
| − | ORGANISM Oryza sativa Japonica Group
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| − | Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
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| − | Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
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| − | clade; Ehrhartoideae; Oryzeae; Oryza.
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| − | |
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| − | Chromosome = [[:category:Japonica Chromosome 5|Chromosome 5]]|
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| − | AP = Chromosome 5:27634371..27639802|
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| − | CDS = 27634761..27634915,27635631..27635696,27635877..27635936,27636331..27636421,27636799..27636853<br>,27636943..27637022,27637136..27637249,27637332..27637416,27637943..27638034<br>,27638578..27638831,27638928..27639016,27639434..27639561|
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| − | GCID = <gbrowseImage1>
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| − | name=NC_008398:27634371..27639802
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| − | source=RiceChromosome05
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| − | preset=GeneLocation
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| − | </gbrowseImage1>|
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| − | GSID = <gbrowseImage2>
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| − | name=NC_008398:27634371..27639802
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| − | source=RiceChromosome05
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| − | preset=GeneLocation
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| − | </gbrowseImage2>|
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| − | CDNA = <cdnaseq>atggaggcgaagcagaggacgaggcatcgcgacggagaggagaggaggctcgtagcggcggcggatggcggcgccgaggagtacgacccatggacggcgtggctctacaagccgcacaccatctcggtgctcctcgtcggtgcttgccttctaatttgggcaagtggagcgcttgatccagagggtgcttcttatcacagtagtgcgacatctattaagaggggtgtctgggctatgattgcagtcttccttgcttactgcactctccaagcaccttcaacgatacttattaggccccatcctgctgtctggcgcctggtgcacggactggctgtcgtttaccttgtcgctctagcctttcttcttttccagaaccgcgatgatgctcgccagttcatgaaacacctttaccctgatcttggagttgaattgccggagagatcttatggagctgactgccgtctgtatgttccggaaaaccctaaaaacaagttcataaatatttatacattatttgacgaatttgtggttgcccatattttgggttggtggggtaaagccgtaatgatacgaaaccagcttcttctttgggtcctctcgattggtttcgagctaatggagcttacattcagacatatgttgccaaactttaatgagtgctggtgggatagtattattttggatatcttgatctgcaactggtttggtatatgggcggggatgcacacagtccgttactttgacggtaaaacatatgagtgggttggactgagccgacagccaagcatcatgggcaaggtgaaaagatcactgagccagttcacccctgcacaatgggacaaggatcaatggtaccctttcatggggccgttgcggttcgttcaagtgttgttcctgtgcgttgttttcatgacagtggagctcaacacattttttcttaaattttgcctctggattcctccaaggaatcctttggttgtgtacagattaatcctatggtggttgattgctattccaaccatccgtgagtacaactcctacttgcaaaacagcaaaccggtgaagaaggttggggctttttgctggctctctctagctatatgcattgtagagcttcttatatgtatgaagtttggacatgggctgtttcatgatcccatgcctacttggttgatcatcttttggagttcggtgggggtagcactagtggttttcttgcttgcatggtcttggaggaatcacctgaaatatcagagaaagcggctataa</cdnaseq>|
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| − | AA = <aaseq>MEAKQRTRHRDGEERRLVAAADGGAEEYDPWTAWLYKPHTISVL LVGACLLIWASGALDPEGASYHSSATSIKRGVWAMIAVFLAYCTLQAPSTILIRPHPA VWRLVHGLAVVYLVALAFLLFQNRDDARQFMKHLYPDLGVELPERSYGADCRLYVPEN PKNKFINIYTLFDEFVVAHILGWWGKAVMIRNQLLLWVLSIGFELMELTFRHMLPNFN ECWWDSIILDILICNWFGIWAGMHTVRYFDGKTYEWVGLSRQPSIMGKVKRSLSQFTP AQWDKDQWYPFMGPLRFVQVLFLCVVFMTVELNTFFLKFCLWIPPRNPLVVYRLILWW LIAIPTIREYNSYLQNSKPVKKVGAFCWLSLAICIVELLICMKFGHGLFHDPMPTWLI IFWSSVGVALVVFLLAWSWRNHLKYQRKRL</aaseq>|
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| − | DNA = <dnaseqindica>391..545#1261..1326#1507..1566#1961..2051#2429..2483#2573..2652#2766..2879#2962..3046#3573..3664#4208..4461#4558..4646#5064..5191#tcatccaacccaagtacccaacccgcgtcgtcatcgtcgtcttcctacttcctcctcctccccaccccacgcacgcgcgtgcggcgccgatcgcggccgacgccgagatttccgcgtgcgcgtggagatagggcacacccgttctctctctccctctctcgggctctgaagtaatccttcgcttctgcttctttcttcttctgcatgtcctgtctgtccccctcgtctcgtctcgtctcgtctcgtctcttgctttcgcctctcctgacctgaccgcgacaatccgtggtggttatatcgcgcaggcgcttcgcttggtttggcgtgttgtttggctgccgcggcagttttctcctgtcacatatcgcagagggttctggaagtgggaacatggaggcgaagcagaggacgaggcatcgcgacggagaggagaggaggctcgtagcggcggcggatggcggcgccgaggagtacgacccatggacggcgtggctctacaagccgcacaccatctcggtgctcctcgtcggtgcttgccttctaatgtaagtgctcttccacgattaaatttcacaaattcgtttttatttttttttctcgctttgctgcctctgcctgtgacttaagggctaaggctgtaaatttcttggtgggagaactggaaaaaaaggccttgttgtgttggggcaaagtacaattcaacagatgccagggcttctgcctatcaggtgcatcaacagtgtcagagttgaaagcagggcatgtagtactcattgtgatttttttactttctctgaaagaaaaaggtgaaatttttgggtcgggaaatttagtgattttgtgccagcaagttgaggtttctgaatgattgaacgaacatgaaagcatggcagtctggttcagcaaggctagcaaacaaccgagcgtacgagtggctgaggagaatcggaatcatgttcaagaatctcgtgcgaaagcttccctagaacaatcattttttcccatatcatttttagcaggtgcaaattacatgggaagtacattcctctcgtcccttttttttaactgtagaaacggctcaccaagaactttattgttgctattgcttcatgaaaggattaagcatataactttgtgtctgaaacaaccattagctaggctaggctgttgagtggttggttgtgaacttgctggggcgttctccttttcactttctcgtggtgctgtctgatttcatgttcaatcatgttcccgtttcagttgggcaagtggagcgcttgatccagagggtgcttcttatcacagtagtgcgacatctattaagaggtaatgaccgaaaaccttttttttttactttactggaaagtgatgcaatcatctaagggaactctggttacttctctactttgattgtgtcttttggacttgagctgttctcctctcaccactgcacaatgattgggagagagtgacactgactcatattttctcactgacactctccaggggtgtctgggctatgattgcagtcttccttgcttactgcactctccaagcaccttcaacgtgagttagcccccctcttcttatatgtatttcaaacattggaactatgttagtgcagtggctgtcgttgtcaggcatttttctttaagtagggaaattgaaggttttctgcttcaaagaggtgacccacgtgttactgtatgagtgagaccatatagtggccaattctcttgaaagtgtagggcttattattcgtcaggttgtttttaatggtgacacaaaaattaccttgagggacccaataccaatgccattctcatgctgtttttgtacttgctacttttgtcttctttatctccttgggttcataacaatttgagaacctgtaaatctactgtgcatctctaatatttttgcatgatgatgattcaatgttctggcaatattgtcacaggatacttattaggccccatcctgctgtctggcgcctggtgcacggactggctgtcgtttaccttgtcgctctagcctttcttcttttccaggttagttgttactattgaaaaattcatacatcgataaagtaggacaaaaaatgccctttcaataatatcccatgcacgtaaaatgttagaaaatggcgggagatgtctattattcatagggttattttgggaagttcttacaggagcgtgaagtggcaagtatttgtgaaagaaaaaacttgagatccaagcaaatgcaaattgtcttgtcaaaaaaaaaagttggaagtcttgtatccaaactatttgattatcaaaattcagtataaatggcattttttcaagactcttgagcgaggggcctcaactacgtagagatatgctacatcttggatatcttgtactgtaataattcttatttctttctcgttttccagaaccgcgatgatgctcgccagttcatgaaacacctttaccctgatcttggagttggtaatatctttacatcgaaaggatttacactggaaattaaatattgtagaagaaatggtgctgacaaacatttctgtacactaatgcagaattgccggagagatcttatggagctgactgccgtctgtatgttccggaaaaccctaaaaacaagttcataaatatttatgtatgatttttcatttgaaccttcctatttcatgctctggtttttgttattattgtagttattatcattgttattaccccatcatatttgacggtttgatatacatataggagacattatttgacgaatttgtggttgcccatattttgggttggtggggtaaagccgtaatgatacgaaaccagcttcttctttgggtcctctcgattggtttcgagctaatggaggttagatatggttactcaaccttttgttatcttttacttgttatctctaacaaggtattgtgcttattatgaccttttgcagcttacattcagacatatgttgccaaactttaatgagtgctggtgggatagtattattttggatatcttgatctgcaactggtttggtaggacaatcggagacagctgactttcatgttgtatacattcagtgacattgatgatacattttttacatctaaagttattagtaaaacaaagttaggcagattaaaataatatagtgtttttgggtttgcatattttatacagtttgtttggatctaactcttatcaactgaacatgataatatagaaaatgaatagcgatgtgatgtatctgagagttttaagaagaaaacttagacctgatgcacatgcatactgctgacattaaacacttactgtttatgaagtaatataatttatcttcttctatcagttcatgggttgaaagcaatctttctatttcagtaaccagttattgaaagagtacctgcatatgcacgttagggtcaaaagtgacaagtaaatgtgaatggaggaagaacaaatctgccatataagcctcactcttagcagtaggctatggagtgttttatgtgttcagcttcatctaaatattttggctgaaatgctaatattcttattcaggtatatgggcggggatgcacacagtccgttactttgacggtaaaacatatgagtgggttggactgagccgacagccaagcatcatgggcaaggtatgttaccaaacaaacaaattgtttcttatttgcaaataggttagtacctttttttaggactgttcttttaatcttcattcatcatggcccttaggcctagattttttttttgtacctgctgaattttcctgggctattgtgctttttgtaagaatttcctcatcgattctttcttgagttacgacttacaagagcctcttgctactgctaagtccatgggagcattctataacaatgcagtcgctgagcaaaagtaaatcactttgacttcttgtctgtagtacgccaaacaacatgtgaggttttcccagtgggactgtattgatttctgttgctctgtccaaactctaaactgtcaattgccattattgatacctgacttcataatttattttgttgtataccattctggcattcagtgtgttttatctgattggactatatgcacatttgtgcagttacattctgcttcagactgcatactatagttaggttgaacccatttacagtttaccgaccttcctttacttgcatatgcaggtgaaaagatcactgagccagttcacccctgcacaatgggacaaggatcaatggtaccctttcatggggccgttgcggttcgttcaagtgttgttcctgtgcgttgttttcatgacagtggagctcaacacattttttcttaaattttgcctctggattcctccaaggaatcctttggttgtgtacagattaatcctatggtggttgattgctattccaaccatccgtgagtacaactcctacttgcaaaacaggttatcccaatttccctgcacgcccaatagcattgcacattttttacaattttacttaatggcttgctaatatatattttttcatggtcacaccagcaaaccggtgaagaaggttggggctttttgctggctctctctagctatatgcattgtagagcttcttatatgtatgaagtttggacatggtgagttactctcttctgacatcatcatcacttgtgaaataattgatctgatttgaaaaattaccttatggtcgcttgattttctcgtgatatatactactccctccggttctataataattaacgttttggacaaagttaaggtcaaacttttataactttgaccatcaataactttaaaaatatttagtttaaagaaactagaacaacatatatagatttgtctttcaaaacactataataaaagtaaacatgtatttatttattgtatatattataatagaaaaataagatcaaaggtatattttgtagaccgtgtcattgtctaaaacatcaattaaaatgaaaccggagggagtagtatatatttggaacattaaatgttaagagtgcctaacttttttgtaatctggatagggctgtttcatgatcccatgcctacttggttgatcatcttttggagttcggtgggggtagcactagtggttttcttgcttgcatggtcttggaggaatcacctgaaatatcagagaaagcggctataatggattaatttttcggaagattttcgactctggacccccttcaaacttgtttcttcttcttgataggatgtacatatgtgatgggtgatgatgtaacttaaatgtgatgggtaattatgtaactttaaatcctaggataaagcaattttcagctacatttcacattatgttttacaatagccaattgttgcctcgcaagagggatggctaatttcagattaataagtgaaatattaccatg</dnaseqindica>|
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| − | Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001062804.1 RefSeq:Os05g0554400]|
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| − | }}
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| | [[Category:Genes]] | | [[Category:Genes]] |
| | [[Category:Japonica mRNA]] | | [[Category:Japonica mRNA]] |
Please input one-sentence summary here.
Annotated Information
Function
In vascular plants, the regulation of stem cell niche determines development of aerial shoot which consists of stems and lateral organs. Intercalary meristem (IM) controls internode elongation in rice and other grasses, however little attention has been paid to the underlying mechanism of stem cell maintenance. Here, we investigated the stem development in rice and showed that the Shortened Uppermost Internode 1 (SUI1) family of genes are pivotal for development of rice stems. We demonstrated that SUI-family genes regulate the development of IM for internode elongation and also the cell expansion of the panicle stem rachis in rice.
Expression
The SUI-family genes encoded base-exchange types of phosphatidylserine synthases (PSSs), which possessed enzymatic activity in a yeast complementary assay. Overexpression of SUI1 and SUI2 caused outgrowths of internodes during vegetative development, and we showed that expression patterns of Oryza Sativa Homeobox 15 (OSH15) and Histone4 were impaired. Furthermore, genome-wide gene expression analysis revealed that overexpression and RNA knockdown of SUI-family genes affected downstream gene expression related to phospholipid metabolic pathways.Moreover, using Ultra-performance liquid chromatography–quadrupole time of flight-mass spectrometry, we analyzed PS contents in different genetic backgrounds of rice and showed that the quantity of very long chain fatty acids PS is affected by transgene of SUI-family genes. Our study reveals a new mechanism conveyed by the SUI1 pathway and provides evidence to link lipid metabolism with plant stem cell maintenance
Evolution
Overexpression of SUI1 and SUI2 caused outgrowths of internodes during vegetative development, and we showed that expression patterns of Oryza Sativa Homeobox 15 (OSH15) and Histone4 were impaired.
Labs working on this gene
1. National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Graduate School of the Chinese Academy of Sciences, Chinese Academy of Sciences, Shanghai, 200032, China.
2. Department of Cell Biology and Molecular Genetics, University of Maryland, College Park, Maryland, USA.
References
1. Hengfu Yin;Peng Gao;Chengwu Liu;Jun Yang;Zhongchi Liu;Da Luo
SUI-family genes encode phosphatidylserine synthases and regulate stem development in rice
Planta, 2013, 237(1): 15-27
Structured Information
