Difference between revisions of "Os06g0133000"
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==Annotated Information== | ==Annotated Information== | ||
===Function=== | ===Function=== | ||
| − | + | The Waxy (Wx) gene, which controls amylose synthesis in endosperm and pollen, has been well studied in rice and in maize. The regulation of the rice Wx gene is characterized by two functional alleles which are defined on the basis of the amount of Wx protein that accumulates in the mature seeds (Sano 1984). The allele that controls the production of lower amounts of Wx protein is Wxb, | |
| − | The Waxy | + | which is mainly distributed in O. sativa Japonica. The other allele, Wxa, produces levels of Wx protein about 10-fold higher than Wxb and is widely distributed in domestic rice, including O. sativa Indica, O. glaberrima, and their wild progenitors. The evolutionary relationships of the species in the genus Oryza indicate that Wxb in O. sativa Japonica has evolved from Wxa of O. rufipogon. Previously, posttranscriptional regulation has been suggested as the cause of the quantitative difference in Wx protein between Wxa and Wxb alleles of cultivated rice. |
===Expression=== | ===Expression=== | ||
| − | + | The alleles at the wx locus, Wxa and Wxb, were initially defined on the basis of the amount of Wx protein that is contained in the mature seed. Seeds with Wxa contain levels of Wx protein about 10-fold higher than those with Wxb. To examine whether this quantitative difference is regulated at the mRNA levels, we carried out Northern blot analysis using one allele of Wxb from O. sativa Japonica (T65) and three alleles from O. sativa Indica, O. rufipogon, and O. glaberrima. All rice strains with Wxa analyzed were congenic lines, in which each wx locus was introduced from the original strains into the genetic background of O.sativa Japonica (T65). | |
| − | + | Total RNA was isolated from developing seeds (10dap) and analyzed by Northern blot using the Wx cDNA probe. The result clearly indicated that the steady-state levels of the mature transcript from rice with Wxa were much higher than those from rice with Wxb. In addition to the mature transcript, an extra band was detected in the lane of O. sativa Japonica . This band corresponds to the precursor of Wx transcripts, which contains the unspliced first intron in the region upstream of the ATG codon, as pointed out previously. The extra band was not detected in the lanes of rice with Wxa. | |
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===Evolution=== | ===Evolution=== | ||
Please input evolution information here. | Please input evolution information here. | ||
| − | + | 59.3% variance of amylose content was attributed to the polymorphism of Wx gene revealed by RM190, while 56.1% and 24.6% of the variances in amylose content and gel consistency were respectively to the polymorphism of Wx gene revealed by 484/W2R-ACC I. Furthermore, with both SSR and CAPS markers, 72.4% of the variance in amylose content could be explained[8]. | |
==Labs working on this gene== | ==Labs working on this gene== | ||
| Line 49: | Line 48: | ||
==References== | ==References== | ||
| − | + | 1. Yan Su;Yuchun Rao;Shikai Hu;Yaolong Yang;Zhenyu Gao;Guanghen Zhang;Jian Liu;Jiang Hu;Meixian Yan;Guojun Dong;Li Zhu;Longbiao Guo;Qian Qian;Dali Zeng | |
| − | |||
| − | |||
Map-based cloning proves qGC-6, a major QTL for gel consistency of japonica/indica cross, responds by Waxy in rice (Oryza sativa L.) | Map-based cloning proves qGC-6, a major QTL for gel consistency of japonica/indica cross, responds by Waxy in rice (Oryza sativa L.) | ||
| − | Theoretical and Applied Genetics, 2011, 123(5): 859-867 | + | Theoretical and Applied Genetics, 2011, 123(5): 859-867 |
2.Ming-Zhou Zhang;Jie-Hong Fang;Xia Yan;Jun Liu;Jin-Song Bao;Gunnel Fransson;Roger Andersson;Christer Jansson;Per Åman;Chuanxin Sun | 2.Ming-Zhou Zhang;Jie-Hong Fang;Xia Yan;Jun Liu;Jin-Song Bao;Gunnel Fransson;Roger Andersson;Christer Jansson;Per Åman;Chuanxin Sun | ||
Molecular insights into how a deficiency of amylose affects carbon allocation -- carbohydrate and oil analyses and gene expression profiling in the seeds of a rice waxy mutant | Molecular insights into how a deficiency of amylose affects carbon allocation -- carbohydrate and oil analyses and gene expression profiling in the seeds of a rice waxy mutant | ||
| − | BMC Plant Biology, 2012, 12: 230 | + | BMC Plant Biology, 2012, 12: 230 |
3. 姚姝;陈涛;张亚东;朱镇;赵凌;赵庆勇;周丽慧;王才林 | 3. 姚姝;陈涛;张亚东;朱镇;赵凌;赵庆勇;周丽慧;王才林 | ||
分子标记辅助选择聚合水稻暗胚乳突变基因Wx-mq和抗条纹叶枯病基因Stv-bi | 分子标记辅助选择聚合水稻暗胚乳突变基因Wx-mq和抗条纹叶枯病基因Stv-bi | ||
| − | 中国水稻科学, 2010, 24(4): 341-347 | + | 中国水稻科学, 2010, 24(4): 341-347 |
4. Zhixi Tian;Qian Qian;Qiaoquan Liu;Meixian Yan;Xinfang Liu;Changjie Yan;Guifu Liu;Zhenyu Gao;Shuzhu Tang;Dali Zeng;Yonghong Wang;Jianming Yu;Minghong Gu;Jiayang Li | 4. Zhixi Tian;Qian Qian;Qiaoquan Liu;Meixian Yan;Xinfang Liu;Changjie Yan;Guifu Liu;Zhenyu Gao;Shuzhu Tang;Dali Zeng;Yonghong Wang;Jianming Yu;Minghong Gu;Jiayang Li | ||
Allelic diversities in rice starch biosynthesis lead to a diverse array of rice eating and cooking qualities | Allelic diversities in rice starch biosynthesis lead to a diverse array of rice eating and cooking qualities | ||
| − | Proceedings of the National Academy of Sciences, 2009, 106(51): 21760-21765 | + | Proceedings of the National Academy of Sciences, 2009, 106(51): 21760-21765 |
5. P. Jayamani;S. Negrão;C. Brites;M.M. Oliveira | 5. P. Jayamani;S. Negrão;C. Brites;M.M. Oliveira | ||
Potential of Waxy gene microsatellite and single-nucleotide polymorphisms to develop japonica varieties with desired amylose levels in rice (Oryza sativa L.) | Potential of Waxy gene microsatellite and single-nucleotide polymorphisms to develop japonica varieties with desired amylose levels in rice (Oryza sativa L.) | ||
| − | Journal of Cereal Science, 2007, 46(2): 178-186. | + | Journal of Cereal Science, 2007, 46(2): 178-186. |
6. 张娅丽; 许明辉; 曾亚文; 姚春馨; 陈善娜 | 6. 张娅丽; 许明辉; 曾亚文; 姚春馨; 陈善娜 | ||
云南地方稻种Wx基因第一内含子供体+1位碱基变异与直链淀粉含量的关系 | 云南地方稻种Wx基因第一内含子供体+1位碱基变异与直链淀粉含量的关系 | ||
| − | 中国水稻科学, 2007, 21(1): 20-24 | + | 中国水稻科学, 2007, 21(1): 20-24 |
7. 刘玉花; 栾丽; 龙文波; 王兴; 孔繁伦; 何涛; 涂升斌 | 7. 刘玉花; 栾丽; 龙文波; 王兴; 孔繁伦; 何涛; 涂升斌 | ||
同源四倍体和二倍体水稻Wx基因与淀粉品质的遗传关系 | 同源四倍体和二倍体水稻Wx基因与淀粉品质的遗传关系 | ||
| − | 中国水稻科学, 2007, 21(2): 143-149 | + | 中国水稻科学, 2007, 21(2): 143-149 |
8. WAN Ying-xiu;DENG Qi-ming;WANG Shi-quan;LIU Ming-wei;ZHOU Hua-qiang;LI Ping | 8. WAN Ying-xiu;DENG Qi-ming;WANG Shi-quan;LIU Ming-wei;ZHOU Hua-qiang;LI Ping | ||
Genetic Polymorphism of Wx Gene and Its Correlation with Main Grain Quality Characteristics in Rice | Genetic Polymorphism of Wx Gene and Its Correlation with Main Grain Quality Characteristics in Rice | ||
| − | Rice Science, 2007, 14(2): 85-93 | + | Rice Science, 2007, 14(2): 85-93 |
9. 陈刚; 王忠; 刘巧泉; 熊飞; 顾蕴洁; 顾国俊 | 9. 陈刚; 王忠; 刘巧泉; 熊飞; 顾蕴洁; 顾国俊 | ||
转反义Wx基因水稻颖果的发育及物质积累 | 转反义Wx基因水稻颖果的发育及物质积累 | ||
| − | 中国水稻科学, 2006, 20(3): 277-282 | + | 中国水稻科学, 2006, 20(3): 277-282 |
10. 万映秀;邓其明;王世全;刘明伟;周华强;李平 | 10. 万映秀;邓其明;王世全;刘明伟;周华强;李平 | ||
水稻Wx基因的遗传多态性及其与主要米质指标的相关性分析 | 水稻Wx基因的遗传多态性及其与主要米质指标的相关性分析 | ||
| − | 中国水稻科学, 2006, 20(6): 603-609 | + | 中国水稻科学, 2006, 20(6): 603-609 |
11. CHEN Gang; WANG Zhong; LIU Qiao-quan; XIONG Fei; GU Yun-jie; GU Guo-jun | 11. CHEN Gang; WANG Zhong; LIU Qiao-quan; XIONG Fei; GU Yun-jie; GU Guo-jun | ||
Development and Substance Accumulation of Caryopsis in Transgenic Rice with Antisense Wx Gene | Development and Substance Accumulation of Caryopsis in Transgenic Rice with Antisense Wx Gene | ||
| − | Rice Science, 2006, 13(2): 106-112 | + | Rice Science, 2006, 13(2): 106-112 |
12. ZENG Rui-zhen; ZHANG Ze-min; HE Feng-hua; XI Zhang-ying; Akshay TALUKDAR; SHI Jun-qiong; QIN Li-jun; HUANG Chao-feng; ZHANG Gui-quan | 12. ZENG Rui-zhen; ZHANG Ze-min; HE Feng-hua; XI Zhang-ying; Akshay TALUKDAR; SHI Jun-qiong; QIN Li-jun; HUANG Chao-feng; ZHANG Gui-quan | ||
Identification of Multiple Alleles at the Wx Locus and Development of Single Segment Substitution Lines for the Alleles in Rice | Identification of Multiple Alleles at the Wx Locus and Development of Single Segment Substitution Lines for the Alleles in Rice | ||
| − | Rice Science, 2006, 13(1): 9-14 | + | Rice Science, 2006, 13(1): 9-14 |
13. 曾瑞珍; 张泽民; 何风华; 席章营; Akshay TALUKDAR; 施军琼; 秦利军; 黄朝锋; 张桂权 | 13. 曾瑞珍; 张泽民; 何风华; 席章营; Akshay TALUKDAR; 施军琼; 秦利军; 黄朝锋; 张桂权 | ||
水稻Wx复等位基因的鉴定及单片段代换系的建立 | 水稻Wx复等位基因的鉴定及单片段代换系的建立 | ||
| − | 中国水稻科学, 2005, 19(6): 495-500 | + | 中国水稻科学, 2005, 19(6): 495-500 |
14. MAO Xing-xue; LIU Yan-zhuo; XIAO Xin; CHEN Jian-wei; LUO Wen-yong; LI Xiao-fang | 14. MAO Xing-xue; LIU Yan-zhuo; XIAO Xin; CHEN Jian-wei; LUO Wen-yong; LI Xiao-fang | ||
A One-Step PCR Method for Detecting the First Base of Splice Donor of Wx Intron 1 in Rice | A One-Step PCR Method for Detecting the First Base of Splice Donor of Wx Intron 1 in Rice | ||
| − | Rice Science, 2004, 11(5-6): 342-344 | + | Rice Science, 2004, 11(5-6): 342-344 |
15. Yamanaka-S;Nakamura-I;Watanabe-K-N;Sato-Y-I | 15. Yamanaka-S;Nakamura-I;Watanabe-K-N;Sato-Y-I | ||
Identification of SNPs in the waxy gene among glutinous rice cultivars and their evolutionary significance during the domestication process of rice | Identification of SNPs in the waxy gene among glutinous rice cultivars and their evolutionary significance during the domestication process of rice | ||
| − | Theoretical and Applied Genetics, 2004, 108(7): 1200-1204 | + | Theoretical and Applied Genetics, 2004, 108(7): 1200-1204 |
16. Zhongyi Li;Fei Sun;Shoumin Xu;Xiusheng Chu;Y. Mukai;M. Yamamoto;Shahjahan Ali;Lynette Rampling;Behjat Kosar-Hashemi;Sadequr Rahman;Matthew K. Morell | 16. Zhongyi Li;Fei Sun;Shoumin Xu;Xiusheng Chu;Y. Mukai;M. Yamamoto;Shahjahan Ali;Lynette Rampling;Behjat Kosar-Hashemi;Sadequr Rahman;Matthew K. Morell | ||
The structural organisation of the gene encoding class II starch synthase of wheat and barley and the evolution of the genes encoding starch synthases in plants | The structural organisation of the gene encoding class II starch synthase of wheat and barley and the evolution of the genes encoding starch synthases in plants | ||
| − | Functional & Integrative Genomics, 2003, 3(1-2): 76-85 | + | Functional & Integrative Genomics, 2003, 3(1-2): 76-85 |
17. Y. Sato;T. Nishio | 17. Y. Sato;T. Nishio | ||
Mutation detection in rice waxy mutants by PCR-RF-SSCP | Mutation detection in rice waxy mutants by PCR-RF-SSCP | ||
| − | Theoretical and Applied Genetics, 2003, 107: 560-567 | + | Theoretical and Applied Genetics, 2003, 107: 560-567 |
18. Hiroyuki Sato; Yasuhiro Suzuki; Makoto Sakai and Tokio Imbe | 18. Hiroyuki Sato; Yasuhiro Suzuki; Makoto Sakai and Tokio Imbe | ||
Molecular Characterization of Wx-mq, a Novel Mutant Gene for Low-amylose Content in Endosperm of Rice (Oryza sativa L.) | Molecular Characterization of Wx-mq, a Novel Mutant Gene for Low-amylose Content in Endosperm of Rice (Oryza sativa L.) | ||
| − | Breeding Science, 2002, 52(2): 131-135 | + | Breeding Science, 2002, 52(2): 131-135 |
19. 于恒秀; 刘巧泉; 陈秀花; 陆美芳; 王兴稳; 王宗阳; 顾铭洪 | 19. 于恒秀; 刘巧泉; 陈秀花; 陆美芳; 王兴稳; 王宗阳; 顾铭洪 | ||
根癌农杆菌介导的水稻转化系统的优化及转反义Wx基因植株的获得 | 根癌农杆菌介导的水稻转化系统的优化及转反义Wx基因植株的获得 | ||
| − | 中国水稻科学, 2002, 16(4): 304-310 | + | 中国水稻科学, 2002, 16(4): 304-310 |
20. Ichiho Mikami;Munetoshi Aikawa;Hiro-Yuki Hirano and Yoshio Sano | 20. Ichiho Mikami;Munetoshi Aikawa;Hiro-Yuki Hirano and Yoshio Sano | ||
Altered tissue-specific expression at the Wx gene of the opaque mutants in rice | Altered tissue-specific expression at the Wx gene of the opaque mutants in rice | ||
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Differential regulation of waxy gene expression in rice endosperm | Differential regulation of waxy gene expression in rice endosperm | ||
Theoretical and Applied Genetics, 1984, 68(5): 467-473.</ref> | Theoretical and Applied Genetics, 1984, 68(5): 467-473.</ref> | ||
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==Structured Information== | ==Structured Information== | ||
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[[Category:Genes]] | [[Category:Genes]] | ||
[[Category:Japonica mRNA]] | [[Category:Japonica mRNA]] | ||
Latest revision as of 08:04, 12 June 2015
Please input one-sentence summary here.
Contents
Annotated Information
Function
The Waxy (Wx) gene, which controls amylose synthesis in endosperm and pollen, has been well studied in rice and in maize. The regulation of the rice Wx gene is characterized by two functional alleles which are defined on the basis of the amount of Wx protein that accumulates in the mature seeds (Sano 1984). The allele that controls the production of lower amounts of Wx protein is Wxb, which is mainly distributed in O. sativa Japonica. The other allele, Wxa, produces levels of Wx protein about 10-fold higher than Wxb and is widely distributed in domestic rice, including O. sativa Indica, O. glaberrima, and their wild progenitors. The evolutionary relationships of the species in the genus Oryza indicate that Wxb in O. sativa Japonica has evolved from Wxa of O. rufipogon. Previously, posttranscriptional regulation has been suggested as the cause of the quantitative difference in Wx protein between Wxa and Wxb alleles of cultivated rice.
Expression
The alleles at the wx locus, Wxa and Wxb, were initially defined on the basis of the amount of Wx protein that is contained in the mature seed. Seeds with Wxa contain levels of Wx protein about 10-fold higher than those with Wxb. To examine whether this quantitative difference is regulated at the mRNA levels, we carried out Northern blot analysis using one allele of Wxb from O. sativa Japonica (T65) and three alleles from O. sativa Indica, O. rufipogon, and O. glaberrima. All rice strains with Wxa analyzed were congenic lines, in which each wx locus was introduced from the original strains into the genetic background of O.sativa Japonica (T65). Total RNA was isolated from developing seeds (10dap) and analyzed by Northern blot using the Wx cDNA probe. The result clearly indicated that the steady-state levels of the mature transcript from rice with Wxa were much higher than those from rice with Wxb. In addition to the mature transcript, an extra band was detected in the lane of O. sativa Japonica . This band corresponds to the precursor of Wx transcripts, which contains the unspliced first intron in the region upstream of the ATG codon, as pointed out previously. The extra band was not detected in the lanes of rice with Wxa.
Evolution
Please input evolution information here.
59.3% variance of amylose content was attributed to the polymorphism of Wx gene revealed by RM190, while 56.1% and 24.6% of the variances in amylose content and gel consistency were respectively to the polymorphism of Wx gene revealed by 484/W2R-ACC I. Furthermore, with both SSR and CAPS markers, 72.4% of the variance in amylose content could be explained[8].
Labs working on this gene
Please input related labs here.
1.State Key Laboratory of Rice Biology, China National Rice Research Institute, 31006 Hangzhou, China
2.Department of Plant Biology & Forest Genetics, Uppsala BioCenter, Swedish University of Agricultural Sciences and Linnean Center for Plant Biology, P.O. Box 7080, SE 75007, Uppsala, Sweden
3.State Key Laboratory of Plant Genomics and National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
4.Institute of Food Crops,Jiangsu Academy of Agricultural Sciences/Jiangsu High Quality Rice R&D Center/Nanjing Branch of Chinese National Center for Rice Improvement, Nanjing 210014,China.
5.College of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming 650201, China.
6.Key Laboratory of Biotechnology Research of Yunnan Province , Yunnan Academy of Agricultural Sciences, Kunming 650223 , China.
7.College of Life Science, Yunnan University , Kunming 650091 , China.
8.Chengdu Institute of Biology , Chinese Academy of Sciences, Chengdu 610041,China.
9.Rice Research Institute, Sichuan Agricultural University, Wenjiang 611130, China.
10.Key Laboratory of Southwest Crop Genetic Resource and Improvement, Ministry of Education, Sichuan Agricultural University, Ya’an 625014, China.
11.Rice Research Institute , Sichuan Agricultural University , Wenjiang 611130 , China.
12.Key Laboratory of Southwest Crop Genetic Resource and Improvement , Ministry of Education, Sichuan Agricultural University , Yacan 625014 , China.
13.Key Laboratory of Crop Genetics and Physiology of Jiangsu Province, Agricultural College, Yangzhou University, Yangzhou 225009, China
References
1. Yan Su;Yuchun Rao;Shikai Hu;Yaolong Yang;Zhenyu Gao;Guanghen Zhang;Jian Liu;Jiang Hu;Meixian Yan;Guojun Dong;Li Zhu;Longbiao Guo;Qian Qian;Dali Zeng
Map-based cloning proves qGC-6, a major QTL for gel consistency of japonica/indica cross, responds by Waxy in rice (Oryza sativa L.) Theoretical and Applied Genetics, 2011, 123(5): 859-867
2.Ming-Zhou Zhang;Jie-Hong Fang;Xia Yan;Jun Liu;Jin-Song Bao;Gunnel Fransson;Roger Andersson;Christer Jansson;Per Åman;Chuanxin Sun
Molecular insights into how a deficiency of amylose affects carbon allocation -- carbohydrate and oil analyses and gene expression profiling in the seeds of a rice waxy mutant BMC Plant Biology, 2012, 12: 230
3. 姚姝;陈涛;张亚东;朱镇;赵凌;赵庆勇;周丽慧;王才林
分子标记辅助选择聚合水稻暗胚乳突变基因Wx-mq和抗条纹叶枯病基因Stv-bi 中国水稻科学, 2010, 24(4): 341-347
4. Zhixi Tian;Qian Qian;Qiaoquan Liu;Meixian Yan;Xinfang Liu;Changjie Yan;Guifu Liu;Zhenyu Gao;Shuzhu Tang;Dali Zeng;Yonghong Wang;Jianming Yu;Minghong Gu;Jiayang Li
Allelic diversities in rice starch biosynthesis lead to a diverse array of rice eating and cooking qualities Proceedings of the National Academy of Sciences, 2009, 106(51): 21760-21765
5. P. Jayamani;S. Negrão;C. Brites;M.M. Oliveira
Potential of Waxy gene microsatellite and single-nucleotide polymorphisms to develop japonica varieties with desired amylose levels in rice (Oryza sativa L.) Journal of Cereal Science, 2007, 46(2): 178-186.
6. 张娅丽; 许明辉; 曾亚文; 姚春馨; 陈善娜
云南地方稻种Wx基因第一内含子供体+1位碱基变异与直链淀粉含量的关系 中国水稻科学, 2007, 21(1): 20-24
7. 刘玉花; 栾丽; 龙文波; 王兴; 孔繁伦; 何涛; 涂升斌
同源四倍体和二倍体水稻Wx基因与淀粉品质的遗传关系 中国水稻科学, 2007, 21(2): 143-149
8. WAN Ying-xiu;DENG Qi-ming;WANG Shi-quan;LIU Ming-wei;ZHOU Hua-qiang;LI Ping
Genetic Polymorphism of Wx Gene and Its Correlation with Main Grain Quality Characteristics in Rice Rice Science, 2007, 14(2): 85-93
9. 陈刚; 王忠; 刘巧泉; 熊飞; 顾蕴洁; 顾国俊
转反义Wx基因水稻颖果的发育及物质积累 中国水稻科学, 2006, 20(3): 277-282
10. 万映秀;邓其明;王世全;刘明伟;周华强;李平
水稻Wx基因的遗传多态性及其与主要米质指标的相关性分析 中国水稻科学, 2006, 20(6): 603-609
11. CHEN Gang; WANG Zhong; LIU Qiao-quan; XIONG Fei; GU Yun-jie; GU Guo-jun
Development and Substance Accumulation of Caryopsis in Transgenic Rice with Antisense Wx Gene Rice Science, 2006, 13(2): 106-112
12. ZENG Rui-zhen; ZHANG Ze-min; HE Feng-hua; XI Zhang-ying; Akshay TALUKDAR; SHI Jun-qiong; QIN Li-jun; HUANG Chao-feng; ZHANG Gui-quan
Identification of Multiple Alleles at the Wx Locus and Development of Single Segment Substitution Lines for the Alleles in Rice Rice Science, 2006, 13(1): 9-14
13. 曾瑞珍; 张泽民; 何风华; 席章营; Akshay TALUKDAR; 施军琼; 秦利军; 黄朝锋; 张桂权
水稻Wx复等位基因的鉴定及单片段代换系的建立 中国水稻科学, 2005, 19(6): 495-500
14. MAO Xing-xue; LIU Yan-zhuo; XIAO Xin; CHEN Jian-wei; LUO Wen-yong; LI Xiao-fang
A One-Step PCR Method for Detecting the First Base of Splice Donor of Wx Intron 1 in Rice Rice Science, 2004, 11(5-6): 342-344
15. Yamanaka-S;Nakamura-I;Watanabe-K-N;Sato-Y-I
Identification of SNPs in the waxy gene among glutinous rice cultivars and their evolutionary significance during the domestication process of rice Theoretical and Applied Genetics, 2004, 108(7): 1200-1204
16. Zhongyi Li;Fei Sun;Shoumin Xu;Xiusheng Chu;Y. Mukai;M. Yamamoto;Shahjahan Ali;Lynette Rampling;Behjat Kosar-Hashemi;Sadequr Rahman;Matthew K. Morell
The structural organisation of the gene encoding class II starch synthase of wheat and barley and the evolution of the genes encoding starch synthases in plants Functional & Integrative Genomics, 2003, 3(1-2): 76-85
17. Y. Sato;T. Nishio
Mutation detection in rice waxy mutants by PCR-RF-SSCP Theoretical and Applied Genetics, 2003, 107: 560-567
18. Hiroyuki Sato; Yasuhiro Suzuki; Makoto Sakai and Tokio Imbe
Molecular Characterization of Wx-mq, a Novel Mutant Gene for Low-amylose Content in Endosperm of Rice (Oryza sativa L.) Breeding Science, 2002, 52(2): 131-135
19. 于恒秀; 刘巧泉; 陈秀花; 陆美芳; 王兴稳; 王宗阳; 顾铭洪
根癌农杆菌介导的水稻转化系统的优化及转反义Wx基因植株的获得 中国水稻科学, 2002, 16(4): 304-310
20. Ichiho Mikami;Munetoshi Aikawa;Hiro-Yuki Hirano and Yoshio Sano
Altered tissue-specific expression at the Wx gene of the opaque mutants in rice Euphytica, 1999, 105(2): 91-97.</ref>
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A single base change altered the regulation of the Waxy gene at the posttranscriptional level during the domestication of rice Molecular Biology and Evolution, 1998, 15(8): 978-987.</ref>
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A naturally occurring functional allele of the rice waxy locus has a GT to TT mutation at the 5' splice site of the first intron The Plant Journal, 1998, 15(1): 133-138.</ref>
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The amylose content in rice endosperm is related to the post-transcriptional regulation of the waxy gene The Plant Journal, 1995, 7(4): 613-622.</ref>
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Temperature effects on the Wx protein level and amylose content in the endosperm of rice Journal of Heredity, 1985, 76(3): 221-222.</ref>
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