Os06g0570100

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Annotated Information

Function

A rice (Oryza sativa L.) semi-dwarf cultivar, Tan-Ginbozu (d35 Tan-Ginbozu ), contributed to the increase in crop productivity in Japan in the 1950s. the semi-dwarf stature of d35 Tan-Ginbozu is caused by a defective early step of gibberellin biosynthesis, which is catalyzed by ent-kaurene oxidase (KO). Homozygote with null alleles of D35showed a severe dwarf phenotype; therefore, d35 Tan-Ginbozu is a weak allele of D35. Os06g0570100 in gene bank its number is AY660665,it is also called tanginbozu dwarf; dwarf-35;ent-kaurene oxidase gene;Gibberellin Biosynthesis Enzyme.In addition, its finding is by discovering that the Tan-Ginbozu is lack of KO enzyme that involves in the begining of sythesizing GA. And its funtion involves in the three step oxidation of ent-kaurene to ent-kaurenoic acid .Moreover,Rice with half dwarf gene d35 is one of the few applications in the production of sd1,and its product can reach 8.26t/hm2.

Expression

Please input expression information here. Isolating from rice genome with 5 KO-like genes,they encode proteins that have high homologous with arabidopsis and pumkin KOs.Expression analysis shows that OsKO2 and OsKO4 have high transcription in different organs,while OsKO1 and OsKO5 have low expression and OsKOL3 maybe a pseudogene.Corresponding to OsKO2,D35 locates in the rice long arm of chromosome 6,linkaged with C214 marker.Gene OsKo2 gene is 8262kb,including 8 exons and 7 introns.D35 is owing of 5 exon 's mononucleotide A change into T,resulting the coding of arginine changed into serine.d35Tan-Ginbozu is just a kind of weak alleles of D35.And lacking of D35 may lead to dwarf seriously.Moreover ,OsKO4 and OsKO5 may consist of systhesizing phytoalexin.

Extension

RDV P2 Protein Interacts with Rice ent-Kaurene Oxidases (Shifeng Zhu et al,.2005)

"RDV-infected rice plants sprayed with GA3 grew almost as tall as the uninfected rice plants. Significantly, application of indole-3-acetic acid (IAA) to the RDV-infected plants failed to restore the height of infected plants "(Shifeng Zhu et al,.2005) GA and Pollen Activities (Tory et al.,2007) (Tory et al.,2007)

Evolution

Please input evolution information here.

D35 is owing to 5 exon 's mononucleotide A change into T,resulting the coding of arginine changed into serine. Also ,it has high homologous with arabidopsis and pumpkin.

Labs working on this gene

a Bioscience and Biotechnology Center, Nagoya University, Chikusa, Nagoya 464-8601, Japan b Japan Society for the Promotion of Science, Chiyoda, Tokyo 102-8472, Japan c Honda Research Institute Japan, Kazusa-Kamatari, Kisarazu-shi, Chiba 292-0818, Japan d Graduate School of Life Science, Tohoku University, Aoba, Sendai 980-8577, Japan e Peking-Yale Joint Center for Plant Molecular Genetics and Agrobiotechnology, National Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life Science, Peking University, Beijing 100871, China f 1Bioscience and Biotechnology Center, Nagoya University, Nagoya, Aichi 464-8601, Japan.

References

1.Shifeng Zhu;Feng Gao;Xuesong Cao;Mao Chen;Gongyin Ye;Chunhong Wei;Yi Li: The Rice Dwarf Virus P2 Protein Interacts with ent-Kaurene Oxidases in Vivo, Leading to Reduced Biosynthesis of Gibberellins and Rice Dwarf Symptoms. Plant Physiology, 2005, 139(4): 1935-1945

2.Hironori Itoh;Tomoko Tatsumi;Tomoaki Sakamoto;Kazuko Otomo;Tomonobu Toyomasu;Hidemi Kitano;Motoyuki Ashikari;Shigeyuki Ichihara and Makoto Matsuoka: A Rice Semi-Dwarf Gene, Tan-Ginbozu (D35), Encodes the Gibberellin Biosynthesis Enzyme, ent-Kaurene Oxidase. Plant Molecular Biology, 2004, 54(4): 533-547

3.Tory Chhun, Koichiro Aya, Kenji Asano, Eiji Yamamoto, Yoichi Morinaka, Masao Watanabe, Hidemi Kitano, Motoyuki Ashikari, Makoto Matsuoka, Miyako Ueguchi-Tanaka: Gibberellin Regulates Pollen Viability and Pollen Tube Growth in Rice. Plant Cell, 2007, 19(12): 3876–3888