Difference between revisions of "Os09g0327600"

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(Created page with " Rice blast, caused by the fungus ''Magnaporthe oryzae'', is one of the most devastating diseases of rice. To understand the molecular basis of Pi5-mediated resistance to ''...")
 
 
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  Rice blast, caused by the fungus ''Magnaporthe oryzae'', is one of the most devastating diseases of rice. To understand the molecular basis of Pi5-mediated resistance to ''M. oryzae'', we cloned the resistance (R) gene at this locus using a map-based cloning strategy. Genetic and phenotypic analyses of 2014 F2 progeny from a mapping population derived from a cross between IR50, a susceptible rice cultivar, and the RIL260 line carrying Pi5 enabled us to narrow down the Pi5 locus to a 130-kb interval. Sequence analysis of this genomic region identified two candidate genes, Pi5-1 and Pi5-2, which encode proteins carrying three motifs characteristic of R genes: an N-terminal coiled-coil (CC) motif, a nucleotide-binding (NB) domain, and a leucine-rich repeat (LRR) motif. In genetic transformation experiments of a susceptible rice cultivar, neither the Pi5-1 nor the Pi5-2 gene was found to confer resistance to ''M. oryzae''. In contrast, transgenic rice plants expressing both of these genes, generated by crossing transgenic lines carrying each gene individually, conferred Pi5-mediated resistance to ''M. oryzae''. Gene expression analysis revealed that Pi5-1 transcripts accumulate after pathogen challenge, whereas the Pi5-2 gene is constitutively expressed. These results indicate that the presence of these two genes is required for rice Pi5-mediated resistance to ''M. oryzae''.
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==Annotated Information==
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===Function===
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===Expression===
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===Evolution===
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==Labs working on this gene==
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==Structured Information==
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[[Category:Genes]][[Category:Oryza Sativa Japonica Group]][[Category:Japonica Chromosome 09]]

Latest revision as of 02:07, 17 June 2015

Please input one-sentence summary here.

Annotated Information

Function

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Expression

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Evolution

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You can also add sub-section(s) at will.

Labs working on this gene

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References

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Structured Information

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