|
|
| (25 intermediate revisions by 3 users not shown) |
| Line 1: |
Line 1: |
| − | Rice blast, caused by the fungus ''Magnaporthe oryzae'', is one of the most devastating diseases of rice. To understand the molecular basis of Pi5-mediated resistance to ''M. oryzae'', we cloned the resistance (R) gene at this locus using a map-based cloning strategy. Genetic and phenotypic analyses of 2014 F2 progeny from a mapping population derived from a cross between IR50, a susceptible rice cultivar, and the RIL260 line carrying Pi5 enabled us to narrow down the Pi5 locus to a 130-kb interval. Sequence analysis of this genomic region identified two candidate genes, Pi5-1 and Pi5-2, which encode proteins carrying three motifs characteristic of R genes: an N-terminal coiled-coil (CC) motif, a nucleotide-binding (NB) domain, and a leucine-rich repeat (LRR) motif. In genetic transformation experiments of a susceptible rice cultivar, neither the Pi5-1 nor the Pi5-2 gene was found to confer resistance to ''M. oryzae''. In contrast, transgenic rice plants expressing both of these genes, generated by crossing transgenic lines carrying each gene individually, conferred Pi5-mediated resistance to ''M. oryzae''. Gene expression analysis revealed that Pi5-1 transcripts accumulate after pathogen challenge, whereas the Pi5-2 gene is constitutively expressed. These results indicate that the presence of these two genes is required for rice Pi5-mediated resistance to ''M. oryzae''.
| + | |
| − | [[File:1.jpg]] | + | Please input one-sentence summary here. |
| | + | |
| | + | ==Annotated Information== |
| | + | ===Function=== |
| | + | Please input function information here. |
| | + | |
| | + | ===Expression=== |
| | + | Please input expression information here. |
| | + | |
| | + | ===Evolution=== |
| | + | Please input evolution information here. |
| | + | |
| | + | You can also add sub-section(s) at will. |
| | + | |
| | + | ==Labs working on this gene== |
| | + | Please input related labs here. |
| | + | |
| | + | ==References== |
| | + | Please input cited references here. |
| | + | |
| | + | ==Structured Information== |
| | + | [[Category:Genes]][[Category:Oryza Sativa Japonica Group]][[Category:Japonica Chromosome 09]] |
Latest revision as of 02:07, 17 June 2015
Please input one-sentence summary here.
Annotated Information
Function
Please input function information here.
Expression
Please input expression information here.
Evolution
Please input evolution information here.
You can also add sub-section(s) at will.
Labs working on this gene
Please input related labs here.
References
Please input cited references here.
Structured Information
