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==The Background of This Project== | ==The Background of This Project== | ||
[[File:|700px|thumb|right|'''Figure 1.''' '' GWA Analysis of Al Tolerance within and across Rice Subpopulations.'']] | [[File:|700px|thumb|right|'''Figure 1.''' '' GWA Analysis of Al Tolerance within and across Rice Subpopulations.'']] | ||
| − | * | + | * Rice (Oryza sativa L.) grain filling is a highly coordinated developmental process. During this period, large amounts of storage compounds are synthesized and transported into the rice endosperm, which are major determinants of the economic value of rice grain and provide nutrients and calories for humans and many other animals. Extensive studies on the mechanisms underlying this process have been carried out in the past two decades. It has been documented that transcription control is a primary mechanism for determining endosperm development. Several enzymes interact with certain key transcription factors to regulate the transcription of nutrient partitioning genes during grain filling, at each developmental stage. Both the participating enzymes and reserve compounds are expressed in appropriate amounts and are tightly regulated both spatially and temporally. Phytohormones are also considered to play important roles in plant development. It has been reported that appropriate concentrations of ethylene, IAA and abscisic acid (ABA) can increase the rate of reserve compound synthesis, leading to higher grain yields. Proteomic and cDNA microarray analyses revealed that the products of grain filling-related genes are associated with several important processes, including biosynthesis, metabolism, transportation, the response to stimuli and signal transduction. These findings, together with observations of the morphological changes that occur rice grain during the filling process, suggest that the accumulation of reserves involves multiple metabolic and regulatory pathways, and the expression of the genes in different pathways is coordinately regulated in a timely manner between different developmental stages during grain filling. In spite of this, the genes and underlying molecular mechanisms controlling rice grain filling remain elusive. |
==Plant Culture & Treatment== | ==Plant Culture & Treatment== | ||
Revision as of 07:53, 22 June 2016
Contents
Project Title
Identification and Expression Analysis of microRNAs at the Grain Filling Stage in Rice( Oryza sativa L.)via Deep Sequencing
The Background of This Project
[[File:|700px|thumb|right|Figure 1. GWA Analysis of Al Tolerance within and across Rice Subpopulations.]]
- Rice (Oryza sativa L.) grain filling is a highly coordinated developmental process. During this period, large amounts of storage compounds are synthesized and transported into the rice endosperm, which are major determinants of the economic value of rice grain and provide nutrients and calories for humans and many other animals. Extensive studies on the mechanisms underlying this process have been carried out in the past two decades. It has been documented that transcription control is a primary mechanism for determining endosperm development. Several enzymes interact with certain key transcription factors to regulate the transcription of nutrient partitioning genes during grain filling, at each developmental stage. Both the participating enzymes and reserve compounds are expressed in appropriate amounts and are tightly regulated both spatially and temporally. Phytohormones are also considered to play important roles in plant development. It has been reported that appropriate concentrations of ethylene, IAA and abscisic acid (ABA) can increase the rate of reserve compound synthesis, leading to higher grain yields. Proteomic and cDNA microarray analyses revealed that the products of grain filling-related genes are associated with several important processes, including biosynthesis, metabolism, transportation, the response to stimuli and signal transduction. These findings, together with observations of the morphological changes that occur rice grain during the filling process, suggest that the accumulation of reserves involves multiple metabolic and regulatory pathways, and the expression of the genes in different pathways is coordinately regulated in a timely manner between different developmental stages during grain filling. In spite of this, the genes and underlying molecular mechanisms controlling rice grain filling remain elusive.
Plant Culture & Treatment
Research Findings
[[File:|700px|thumb|right|Figure 2. Haplotype analysis of the Nrat1 gene region.]]
Labs working on this Project
- State Key Laboratory of Hybrid Rice, Department of Genetics, College of Life Sciences, Wuhan University, Wuhan, People’s Republic of China
Corresponding Author
- Yi Ding(yiding@whu.edu.cn)
