Difference between revisions of "IC4R019-RNA-Seq-2013-23324257"
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==Plant Culture & Treatment== | ==Plant Culture & Treatment== | ||
| − | *In this study, the researchers focused our heterosis research on the late-stage high-vigor trr super-hybrid rice variety, Xieyou 9308,which has a grain yield of up to 12.23 × 103 kg ⋅ hm-2 and was designated as “super rice” by the Chinese Ministry of Agriculture in 2005. Xieyou 9308 was bred by crossing the restorer line R9308 (with 25% japonica genetic components) to the maternal line Xieqingzao B (indica). | + | *In this study, the researchers focused our heterosis research on the late-stage high-vigor trr super-hybrid rice variety, Xieyou 9308,which has a grain yield of up to 12.23 × 103 kg ⋅ hm-2 and was designated as “super rice” by the Chinese Ministry of Agriculture in 2005. Xieyou 9308 was bred by crossing the restorer line R9308 (with 25% japonica genetic components) to the maternal line Xieqingzao B (indica). |
| − | pathways. The researchers expect this genome-wide transcriptome comparison to provide a starting point to understand the causative mechanism of the altered gene expression in the hybrid and the molecular mechanism underlying rice root heterosis. | + | *The researchers used RNA-Seq to investigate the global transcriptomes of roots from Xieyou 9308 and its parents at tillering and heading stages. Differentially expressed transcripts and their expression patterns were analyzed, and several potential candidate transcripts were found to be involved in carbohydrate metabolism and plant hormone signal transduction pathways. |
| + | *The researchers expect this genome-wide transcriptome comparison to provide a starting point to understand the causative mechanism of the altered gene expression in the hybrid and the molecular mechanism underlying rice root heterosis. | ||
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==Research Findings== | ==Research Findings== | ||
Revision as of 11:44, 11 July 2016
Contents
Project Title
- Transcriptome analysis of rice root heterosis by RNA-Seq
The Background of This Project
- Heterosis is a phenomenon in which hybrids exhibit superior performance relative to parental phenotypes. In addition to the heterosis of above-ground agronomic traits on which most existing studies have focused, root heterosis is also an indispensable component of heterosis in the entire plant and of major importance to plant breeding. Consequently, systematic investigations of root heterosis, particularly in reproductive-stage rice,are needed. The recent advent of RNA sequencing technology (RNA-Seq) provides an opportunity to conduct in-depth transcript profiling for heterosis studies.
Plant Culture & Treatment
- In this study, the researchers focused our heterosis research on the late-stage high-vigor trr super-hybrid rice variety, Xieyou 9308,which has a grain yield of up to 12.23 × 103 kg ⋅ hm-2 and was designated as “super rice” by the Chinese Ministry of Agriculture in 2005. Xieyou 9308 was bred by crossing the restorer line R9308 (with 25% japonica genetic components) to the maternal line Xieqingzao B (indica).
- The researchers used RNA-Seq to investigate the global transcriptomes of roots from Xieyou 9308 and its parents at tillering and heading stages. Differentially expressed transcripts and their expression patterns were analyzed, and several potential candidate transcripts were found to be involved in carbohydrate metabolism and plant hormone signal transduction pathways.
- The researchers expect this genome-wide transcriptome comparison to provide a starting point to understand the causative mechanism of the altered gene expression in the hybrid and the molecular mechanism underlying rice root heterosis.
Research Findings
Labs working on this Project
- State Key Laboratory of Rice Biology, China National Rice Research Institute,Hangzhou 310006, China.
- College of Agronomy, Shenyang Agricultural University, Shenyang 110161, China.
- Rice Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, China.
- Rice Research Institute, Guizhou Academy of Agricultural Sciences, Guiyang 550006, China.
Corresponding Author
- caolycgf@mail.hz.zj.cn; & shcheng@mail.hz.zj.cn
