Difference between revisions of "IC4R014-RNA-Seq-2013-23322175"

Revision as of 13:49, 13 July 2016

Transcriptomic analysis of rice (Oryza sativa) endosperm using the RNA-Seq technique

The endosperm plays an important role in seed formation and germination, especially in rice (Oryza sativa). In this project, the researchers used a high-throughput sequencing technique (RNA-Seq) to reveal the molecular mechanisms involved in rice endosperm development.

The endosperm were derived from rice (O. sativa L.ssp. indica cv.9311), which were grown in a greenhouse at Wuhan University, China. Caryopses were tagged at the initiation of pollination. The samples consisted of three replicates arranged in a randomized complete block design. For each replicate, development seed were collected from 15 different plants at 3, 6, and 10 DAP (Fig. 7) during the growing season. The three-stage endosperm was taken from caryopses with microdissection needles under a dissection microscope (Olympus, Tokyo, Japan). The materials were immediately frozen in liquid nitrogen and kept at -80 �C until required (for RNA extractions).

State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan 430072, China

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 ==Plant Culture & Treatment==
+* The endosperm were derived from rice (O. sativa L.ssp. indica cv.9311), which were grown in a greenhouse at Wuhan University, China. Caryopses were tagged at the initiation of pollination. The samples consisted of three replicates arranged in a randomized complete block design. For each replicate, development seed were collected from 15 different plants at 3, 6, and 10 DAP (Fig. 7) during the growing season. The three-stage endosperm was taken from caryopses with microdissection needles under a dissection microscope (Olympus, Tokyo, Japan). The materials were immediately frozen in liquid nitrogen and kept at -80 �C until required (for RNA extractions).
 ==Illumina Sequencing==