Difference between revisions of "IC4R016-RNA-Seq-2013-24215296"
(→Corresponding Author) |
|||
| Line 3: | Line 3: | ||
<br> | <br> | ||
==The Background of This Project== | ==The Background of This Project== | ||
| − | + | *The formation of a zygote by the fusion of egg and sperm involves the two gametic transcriptomes. In flowering plants, the embryo sac embedded within the ovule contains the egg cell, whereas the pollen grain contains two sperm cells inside a supporting vegetative cell. The difficulties of collecting isolated gametes and consequent low recovery of RNA have restricted in-depth analysis of gametic transcriptomes in flowering plants. The researchers isolated living egg cells, sperm cells and pollen vegetative cells from Oryza sativa (rice), and identified transcripts for approximately 36 000 genes by deep sequencing. | |
| + | <br> | ||
==Plant Culture & Treatment== | ==Plant Culture & Treatment== | ||
Revision as of 08:24, 14 July 2016
Contents
Project Title
- Transcriptomes of isolated Oryza sativa gametes characterized by deep sequencing: evidence for distinct sex-dependent chromatin and epigenetic states before fertilization
The Background of This Project
- The formation of a zygote by the fusion of egg and sperm involves the two gametic transcriptomes. In flowering plants, the embryo sac embedded within the ovule contains the egg cell, whereas the pollen grain contains two sperm cells inside a supporting vegetative cell. The difficulties of collecting isolated gametes and consequent low recovery of RNA have restricted in-depth analysis of gametic transcriptomes in flowering plants. The researchers isolated living egg cells, sperm cells and pollen vegetative cells from Oryza sativa (rice), and identified transcripts for approximately 36 000 genes by deep sequencing.
Plant Culture & Treatment
Illumina sequencing
Research Findings
Labs working on this Project
- Department of Plant Biology,University of California, Davis, CA 95616, USA,
- Department of Microbiology and Plant Biology, University of Oklahoma, Norman, OK 73019, USA,
- School of Life Sciences, Lanzhou University, Lanzhou 730000, China, and
- Department of Plant Sciences, University of California, Davis, CA 95616, USA
Corresponding Author
- Sarah N. Anderson:srussell@ou.edu & Venkatesan Sundaresan:sundar@ucdavis.edu
