Difference between revisions of "IC4R016-RNA-Seq-2013-24215296"

Revision as of 08:47, 14 July 2016

Transcriptomes of isolated Oryza sativa gametes characterized by deep sequencing: evidence for distinct sex-dependent chromatin and epigenetic states before fertilization

The formation of a zygote by the fusion of egg and sperm involves the two gametic transcriptomes. In flowering plants, the embryo sac embedded within the ovule contains the egg cell, whereas the pollen grain contains two sperm cells inside a supporting vegetative cell. The difficulties of collecting isolated gametes and consequent low recovery of RNA have restricted in-depth analysis of gametic transcriptomes in flowering plants. The researchers isolated living egg cells, sperm cells and pollen vegetative cells from Oryza sativa (rice), and identified transcripts for approximately 36 000 genes by deep sequencing.

Illumina sequencing by the RNA-seq protocol was performed on RNA samples from three biological replicates for each of the three cell types.Samples were multiplexed and run with six samples per lane on the Illumina HiSeq 2000 DNA sequencer (Illumina, http://www.illumina.com).

Department of Plant Biology,University of California, Davis, CA 95616, USA,
Department of Microbiology and Plant Biology, University of Oklahoma, Norman, OK 73019, USA,
School of Life Sciences, Lanzhou University, Lanzhou 730000, China, and
Department of Plant Sciences, University of California, Davis, CA 95616, USA

Sarah N. Anderson:srussell@ou.edu & Venkatesan Sundaresan:sundar@ucdavis.edu

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 ==Illumina sequencing==
-*Images of the isolated cell types studied are shown in Figure 1. Illumina sequencing by the RNA-seq protocol was performed on RNA samples from three biological replicates for each of the three cell types (see Experimental procedures).
+*Illumina sequencing by the RNA-seq protocol was performed on RNA samples from three biological replicates for each of the three cell types.Samples were multiplexed and run with six samples per lane on the Illumina HiSeq 2000 DNA sequencer (Illumina, http://www.illumina.com).
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 ==Research Findings==