Difference between revisions of "IC4R010-miRNA-2010-19796675"
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==The Background of This Project== | ==The Background of This Project== | ||
| − | *A class of small RNA molecules called microRNAs (miRNAs) has been identified in recent years. MiRNAs are endogenous non-coding | + | *A class of small RNA molecules called microRNAs (miRNAs) has been identified in recent years. MiRNAs are endogenous non-coding RNAs, are 20–22 nucleotides long, and regulate gene expression in eukaryotes ranging from animals to plants [1–6]. |
| − | RNAs, are 20–22 nucleotides long, and regulate gene expression in eukaryotes ranging from animals to plants [1–6]. | + | *Four Dicer-like enzymes (DCL1–DCL4) are encoded in the genome of Arabidopsis thaliana [20]. It has been shown that DCL1 is involved in miRNA accumulation [21]. However, there is no dcl1 mutant available for rice. A recent study has shown that the loss of function of OsDCL1 transformants could be used to identify miRNAs[22]. |
| − | *Four Dicer-like enzymes (DCL1–DCL4) are encoded in the genome of Arabidopsis thaliana [20]. It has been shown that DCL1 is involved in miRNA accumulation [21]. However, there is no dcl1 mutant available for rice. A recent study has shown that the loss of | + | *MiR399 was shown to down-regulate UBC24 mRNA accumulation and to be involved in plant responses to Pi starvation in planta [24,25]. MiR393 has also been shown to inhibit the expression of TIR1 to down-regulated auxin signaling and seedling growth under abiotic stress conditions [4,26]. Moreover, miR159 was shown to involved in hormone signaling and dehydration responses in Arabidopsis [27,28]. However, these observations were all shown in Arabidopsis, few stress-related miRNAs have been discovered in rice.Among miRNAs discovered in rice, only two have been found to be related to abiotic stress, miR393 and miR169g, both up-regulated by dehydration [29]. |
| − | function of OsDCL1 transformants could be used to identify miRNAs[22]. | ||
| − | * | ||
==Plant Culture & Treatment== | ==Plant Culture & Treatment== | ||
Revision as of 12:51, 15 July 2016
Contents
Project Title
- Identification of novel stress-regulated microRNAs from Oryza sativa L.
The Background of This Project
- A class of small RNA molecules called microRNAs (miRNAs) has been identified in recent years. MiRNAs are endogenous non-coding RNAs, are 20–22 nucleotides long, and regulate gene expression in eukaryotes ranging from animals to plants [1–6].
- Four Dicer-like enzymes (DCL1–DCL4) are encoded in the genome of Arabidopsis thaliana [20]. It has been shown that DCL1 is involved in miRNA accumulation [21]. However, there is no dcl1 mutant available for rice. A recent study has shown that the loss of function of OsDCL1 transformants could be used to identify miRNAs[22].
- MiR399 was shown to down-regulate UBC24 mRNA accumulation and to be involved in plant responses to Pi starvation in planta [24,25]. MiR393 has also been shown to inhibit the expression of TIR1 to down-regulated auxin signaling and seedling growth under abiotic stress conditions [4,26]. Moreover, miR159 was shown to involved in hormone signaling and dehydration responses in Arabidopsis [27,28]. However, these observations were all shown in Arabidopsis, few stress-related miRNAs have been discovered in rice.Among miRNAs discovered in rice, only two have been found to be related to abiotic stress, miR393 and miR169g, both up-regulated by dehydration [29].
Plant Culture & Treatment
Research Findings
Labs working on this Project
- National Centre for Plant Gene Research, Key Laboratory of Molecular and Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences,P.O. Box 2707, South 1-3, Zhongguancun, Beijing 100080, P.R. China
- State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, P.R. China
- National Engineering Research Center for Beijing Biochip Technology, Beijing 102206, China
Corresponding Author
- Fan Chen:fchen@genetics.ac.cn.
