Difference between revisions of "IC4R012-Proteomic-2009-19201764"
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==Plant Culture & Treatment== | ==Plant Culture & Treatment== | ||
*Rice (Oryza sativa L. ‘Wuyunjing’ No. 7) seeds were surface-sterilized with 5% (v/v) sodium hypochlorite(NaClO) for 15 min and thoroughly washed in distilled water. Seeds were germinated on moist filter paper. Twenty seeds were randomly placed on the filter paper in 90-mm Petri dishes, and 5 mL of freshly prepared CuSO4.5H2O solution (0–200 mM) was added. Each treatment was performed in triplicate. The seeds were put to germinate for 4–8 d in the dark at 258C with renewal of the Cu solution at 2-d intervals. Seeds were considered to have germinated when the shoots were longer than 2 mm. The radicle length was measured during the exposure period. Embryos, including newly formed shoots, were isolated from the seeds after 6 d for Cu concentration and protein analyses. | *Rice (Oryza sativa L. ‘Wuyunjing’ No. 7) seeds were surface-sterilized with 5% (v/v) sodium hypochlorite(NaClO) for 15 min and thoroughly washed in distilled water. Seeds were germinated on moist filter paper. Twenty seeds were randomly placed on the filter paper in 90-mm Petri dishes, and 5 mL of freshly prepared CuSO4.5H2O solution (0–200 mM) was added. Each treatment was performed in triplicate. The seeds were put to germinate for 4–8 d in the dark at 258C with renewal of the Cu solution at 2-d intervals. Seeds were considered to have germinated when the shoots were longer than 2 mm. The radicle length was measured during the exposure period. Embryos, including newly formed shoots, were isolated from the seeds after 6 d for Cu concentration and protein analyses. | ||
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| + | ==Protein Extraction and 2-D PAGE== | ||
==Research Findings== | ==Research Findings== | ||
Revision as of 05:50, 16 July 2016
Contents
Project Title
- Proteomic identification of small, copper-responsive proteins in germinating embryos of Oryza sativa
The Background of This Project
- Although copper (Cu) is an essential micronutrient for plants and algae, excess Cu is toxic to most plants and can cause a wide range of deleterious effects. To investigate the response of rice (Oryza sativa) to Cu stress, a proteomic approach was used to analyse Cu stress-induced changes in the expression of low molecular-weight proteins in germinating rice seed embryos.
Plant Culture & Treatment
- Rice (Oryza sativa L. ‘Wuyunjing’ No. 7) seeds were surface-sterilized with 5% (v/v) sodium hypochlorite(NaClO) for 15 min and thoroughly washed in distilled water. Seeds were germinated on moist filter paper. Twenty seeds were randomly placed on the filter paper in 90-mm Petri dishes, and 5 mL of freshly prepared CuSO4.5H2O solution (0–200 mM) was added. Each treatment was performed in triplicate. The seeds were put to germinate for 4–8 d in the dark at 258C with renewal of the Cu solution at 2-d intervals. Seeds were considered to have germinated when the shoots were longer than 2 mm. The radicle length was measured during the exposure period. Embryos, including newly formed shoots, were isolated from the seeds after 6 d for Cu concentration and protein analyses.
