Difference between revisions of "IC4R008-miRNA-2011-21113019"

Revision as of 07:29, 16 July 2016

Identification and analysis of seven H2O2-responsive miRNAs and 32 new miRNAs in the seedlings of rice (Oryza sativa L. ssp. indica)

Rice (Oryza sativa L. ssp indica cv. 93-11) seedlings were grown in a growth chamber with 28/21℃ (16 h day/8 h night) and relative humidity of 70% as reported earlier (4). For H2O2 treatment, 12-day-old seedlings were treated with three H2O2 concentrations (0.6, 3.0 and 15.0mM) for 6 h in plastic containers, respectively (4). Seedlings immersed in dd H2O were used as a control. The rice seedlings were snap-frozen in liquid nitrogen, and then stored at -80℃ for RNA extraction.

Laboratory of Molecular Biology and MOE Laboratory of Protein Science, School of Life Sciences, Tsinghua University, Beijing 100084, P. R. China

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 ==Plant Culture & Treatment==
-* Rice (Oryza sativa L. ssp indica cv. 93-11) seedlings were grown in a growth chamber with 28/21�C (16 h day/8 h night) and relative humidity of 70% as reported earlier (4). For H2O2 treatment, 12-day-old seedlings were treated with three H2O2 concentrations (0.6, 3.0 and 15.0mM) for 6 h in plastic containers, respectively (4). Seedlings immersed in dd H2O were used as a control. The rice seedlings were snap-frozen in liquid nitrogen, and then stored at �80�C for RNA extraction.
+* Rice (Oryza sativa L. ssp indica cv. 93-11) seedlings were grown in a growth chamber with 28/21℃ (16 h day/8 h night) and relative humidity of 70% as reported earlier (4). For H2O2 treatment, 12-day-old seedlings were treated with three H2O2 concentrations (0.6, 3.0 and 15.0mM) for 6 h in plastic containers, respectively (4). Seedlings immersed in dd H2O were used as a control. The rice seedlings were snap-frozen in liquid nitrogen, and then stored at -80℃ for RNA extraction.
 ==Research Findings==