Difference between revisions of "IC4R004-Proteomic-2007-17849412"

Revision as of 02:59, 18 July 2016

The seeds of the rice (O. sativa cv. 9311) were peeled off and washed with distilled water three times, and then imbibed in distilled water (the water was changed every 24 h) at 267C with 100 mmol photons and m2 s white light in a 12-h light/ dark cycle (12 h day/12 h night). We recorded the seeds’ germination rate, and the changes of 1000 seeds’ weight were recorded during the germination process. Meanwhile, we collected the seeds at 0, 12, 24, 48, and 72 h after imbibition, and they were stored at 2807C until used for protein extraction.

Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany,

Chinese Academy of Sciences, Beijing, P. R. China

College of Life Sciences, Capital Normal University, Beijing, P. R. China
Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, P. R. China

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 ==Plant Culture & Treatment==
+* The seeds of the rice (O. sativa cv. 9311) were peeled off and washed with distilled water three times, and then imbibed in distilled water (the water was changed every 24 h) at 267C with 100 mmol photons and m2 s white light in a 12-h light/ dark cycle (12 h day/12 h night). We recorded the seeds’ germination rate, and the changes of 1000 seeds’ weight were recorded during the germination process. Meanwhile, we collected the seeds at 0, 12, 24, 48, and 72 h after imbibition, and they were stored at 2807C until used for protein extraction.
 ==Protein Extraction and 2-D PAGE==