Difference between revisions of "IC4R004-Proteomic-2007-17849412"

Revision as of 03:03, 18 July 2016

Although seed germination is a major subject in plant physiological research, there is still a long way to go to elucidate the mechanism of seed germination. Recently, functional genomic strategies have been applied to study the germination of plant seeds. In this project, the researchers conducted a proteomic analysis of seed germination in rice (Oryza sativa indica cv. 9311) – a model monocot.

The seeds of the rice (O. sativa cv. 9311) were peeled off and washed with distilled water three times, and then imbibed in distilled water (the water was changed every 24 h) at 267C with 100 mmol photons and m2 s white light in a 12-h light/ dark cycle (12 h day/12 h night). We recorded the seeds’ germination rate, and the changes of 1000 seeds’ weight were recorded during the germination process. Meanwhile, we collected the seeds at 0, 12, 24, 48, and 72 h after imbibition, and they were stored at 2807C until used for protein extraction.

Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany,

Chinese Academy of Sciences, Beijing, P. R. China

College of Life Sciences, Capital Normal University, Beijing, P. R. China
Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, P. R. China

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 ==The Background of This Project==
+* Although seed germination is a major subject in plant physiological research, there is still a long way to go to elucidate the mechanism of seed germination. Recently, functional genomic strategies have been applied to study the germination of plant seeds. In this project, the researchers conducted a proteomic analysis of seed germination in rice (Oryza sativa indica cv. 9311) – a model monocot.
 ==Plant Culture & Treatment==