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* Three rice varieties, japonica type Moroberekan (highly susceptible to high temperature during anthesis), indica type IR64 (moderately tolerant), and an Aus type N22 (highly tolerant) were chosen for this study. Pregerminated seeds were sown into 2l trays. After 15 d, three seedlings each were transplanted into 10 l pots. For each of the three genotypes, 34 pots were sown. The plants were grown under flooded conditions throughout the crop cycle. There were no other pest or disease problems. | * Three rice varieties, japonica type Moroberekan (highly susceptible to high temperature during anthesis), indica type IR64 (moderately tolerant), and an Aus type N22 (highly tolerant) were chosen for this study. Pregerminated seeds were sown into 2l trays. After 15 d, three seedlings each were transplanted into 10 l pots. For each of the three genotypes, 34 pots were sown. The plants were grown under flooded conditions throughout the crop cycle. There were no other pest or disease problems. | ||
| − | * On the first day of anthesis (i.e. the appearance of anthers), plants were transferred at 08.00 h into growth chambers (Thermoline, Australia) with temperatures gradually increasing from 29 | + | * On the first day of anthesis (i.e. the appearance of anthers), plants were transferred at 08.00 h into growth chambers (Thermoline, Australia) with temperatures gradually increasing from 29 ℃ to 38 ℃ by 09.00 h (2.5 h after dawn) and maintained at 38 ℃ (SD¼0.13) until 15.00 h, with an RH of 75% (SD¼1.10). Immediately after the heat treatment, plants were moved back to the control conditions (29/21 ℃) before being returned to the growth cabinets and exposed to the same conditions the following morning at 09.00 h, i.e. plants were exposed to 2 d of high temperature. |
==Protein Extraction and 2-D PAGE== | ==Protein Extraction and 2-D PAGE== | ||
Revision as of 05:23, 18 July 2016
Contents
Project Title
- Physiological and proteomic approaches to address heat tolerance during anthesis in rice (Oryza sativa L.)
The Background of This Project
- Episodes of high temperature at anthesis, which in rice is the most sensitive stage to temperature, are expected to occur more frequently in future climates. In this project, the morphology of the reproductive organs and pollen number, and changes in anther protein expression, were studied in response to high temperature at anthesis in three rice (Oryza sativa L.) genotypes.
Plant Culture & Treatment
- Plants were grown in a temperature controlled greenhouse maintained at 29/21 ℃ day/night temperature [Actual: 27.7 ℃ (SD¼0.80)/19.9 oC (SD¼0.11)] and RH of 75% [Actual 82.7% (SD¼2.83)] under natural sunlight conditions at the International Rice Research Institute (IRRI), Philippines.
- Three rice varieties, japonica type Moroberekan (highly susceptible to high temperature during anthesis), indica type IR64 (moderately tolerant), and an Aus type N22 (highly tolerant) were chosen for this study. Pregerminated seeds were sown into 2l trays. After 15 d, three seedlings each were transplanted into 10 l pots. For each of the three genotypes, 34 pots were sown. The plants were grown under flooded conditions throughout the crop cycle. There were no other pest or disease problems.
- On the first day of anthesis (i.e. the appearance of anthers), plants were transferred at 08.00 h into growth chambers (Thermoline, Australia) with temperatures gradually increasing from 29 ℃ to 38 ℃ by 09.00 h (2.5 h after dawn) and maintained at 38 ℃ (SD¼0.13) until 15.00 h, with an RH of 75% (SD¼1.10). Immediately after the heat treatment, plants were moved back to the control conditions (29/21 ℃) before being returned to the growth cabinets and exposed to the same conditions the following morning at 09.00 h, i.e. plants were exposed to 2 d of high temperature.
Protein Extraction and 2-D PAGE
Research Findings
Labs working on this Project
- Plant Environment Laboratory, University of Reading, Cutbush Lane, Shinfield, Reading RG2 9AF, UK
- Plant Breeding, Genetics and Biochemistry Division, International Rice Research Institute, DAPO BOX 7777, Metro Manila, Philippines
Corresponding Author
- p.q.craufurd@reading.ac.uk
