Difference between revisions of "IC4R012-GWAS-2016-26552884"
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==Plant Culture & Treatment== | ==Plant Culture & Treatment== | ||
| + | *The seeds of the rice accessions were obtained from the National Rice Research Centre, USA, and bulked in Aberdeen, UK. The nematode screening method was adopted from Shrestha et al., (2007). The accessions were assessed in batches of 40 using 10 temporally separated runs. Each run consisted of three plug trays (LBS, Colne, UK) of 84 wells each [36.5 × 36.5×50 mm (length × width × height)] with each tray containing two replicates of 40 accessions from the RDP1 plus Azucena (tropical japonica rice variety) and Bala (indica rice variety) as reference genotypes. All genotypes were replicated six times,making a total of 252 plants per screening run. Within the three plug trays, a randomized complete block design was used where a block was one half of a tray. The trays were filled with sand and sown directly with two seeds per plug, which were then thinned to one seed per plug after 1 week. Every 2 weeks, another batch of 40 RDP1 accessions plus Azucena and Bala reference plants were sown in a separate screening run. J2 were collected from the roots of 50 mature rice plants as described in Shrestha et al. (2007), except that | ||
| + | where cut galls from these stock plant roots were incubated for 7 days at 30°C in Shrestha et al., (2007), we incubated them for only 3 days at 28°C. | ||
| + | *The M. graminicola inoculum used was originally obtained from CABI (Egham, UK) and has been maintained on rice for 10 years | ||
| + | in Aberdeen. The plants were inoculated with 200 J2 per plant 2 weeks after planting. Two weeks after inoculation, the roots were | ||
| + | carefully removed and washed with tap water, and the nematode galls counted. An incubation period of 2 weeks was chosen to terminate the experiment near the end of one nematode life cycle to avoid secondary galling. The life cycle of M. graminicola is completed in 19 days at 22–29°C in well-drained soil (Pokharel et al., 2010; Kyndt et al., 2012; Ji et al., 2013). The temperature in the growth chamber ranged from 28°C (day) to 25°C (night), with 50–70% humidity, and light of 350 µmol m−2 s−1 photosynthetically active radiation at plant height for 12 h d−1. The plants were watered daily to field capacity and fertilized twice a week with Yoshida’s nutrient solution (Yoshida et al., 1976). One of the runs included 30 randomly selected plants of RDP1 assessed for a second time to confirm the repeatability of the method. | ||
| + | <br> | ||
==Research Findings== | ==Research Findings== | ||
Revision as of 03:26, 19 July 2016
Contents
Project Title
- A genome-wide association study of a global rice panel reveals resistance in Oryza sativa to root-knot nematodes
The Background of This Project
Plant Culture & Treatment
- The seeds of the rice accessions were obtained from the National Rice Research Centre, USA, and bulked in Aberdeen, UK. The nematode screening method was adopted from Shrestha et al., (2007). The accessions were assessed in batches of 40 using 10 temporally separated runs. Each run consisted of three plug trays (LBS, Colne, UK) of 84 wells each [36.5 × 36.5×50 mm (length × width × height)] with each tray containing two replicates of 40 accessions from the RDP1 plus Azucena (tropical japonica rice variety) and Bala (indica rice variety) as reference genotypes. All genotypes were replicated six times,making a total of 252 plants per screening run. Within the three plug trays, a randomized complete block design was used where a block was one half of a tray. The trays were filled with sand and sown directly with two seeds per plug, which were then thinned to one seed per plug after 1 week. Every 2 weeks, another batch of 40 RDP1 accessions plus Azucena and Bala reference plants were sown in a separate screening run. J2 were collected from the roots of 50 mature rice plants as described in Shrestha et al. (2007), except that
where cut galls from these stock plant roots were incubated for 7 days at 30°C in Shrestha et al., (2007), we incubated them for only 3 days at 28°C.
- The M. graminicola inoculum used was originally obtained from CABI (Egham, UK) and has been maintained on rice for 10 years
in Aberdeen. The plants were inoculated with 200 J2 per plant 2 weeks after planting. Two weeks after inoculation, the roots were
carefully removed and washed with tap water, and the nematode galls counted. An incubation period of 2 weeks was chosen to terminate the experiment near the end of one nematode life cycle to avoid secondary galling. The life cycle of M. graminicola is completed in 19 days at 22–29°C in well-drained soil (Pokharel et al., 2010; Kyndt et al., 2012; Ji et al., 2013). The temperature in the growth chamber ranged from 28°C (day) to 25°C (night), with 50–70% humidity, and light of 350 µmol m−2 s−1 photosynthetically active radiation at plant height for 12 h d−1. The plants were watered daily to field capacity and fertilized twice a week with Yoshida’s nutrient solution (Yoshida et al., 1976). One of the runs included 30 randomly selected plants of RDP1 assessed for a second time to confirm the repeatability of the method.
Research Findings
Labs working on this Project
- Institute of Biological and Environmental Sciences, University of Aberdeen, Aberdeen, AB243UU, UK
- Department of Molecular Biotechnology, Ghent University (UGent), Coupure Links 653, B-9000, Ghent, Belgium
- Current address: Department of Crop and Soil Science, Rivers State University of Science and Technology, Nigeria
Corresponding Author
- Adam H. Price:a.price@abdn.ac
