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| − | *A Japanese rice collection of 175 accessions were used in this study (Table S1) (Yonemaru et al., 2012). The Sasanishiki/Habataki | + | *A Japanese rice collection of 175 accessions were used in this study (Table S1) (Yonemaru et al., 2012). The Sasanishiki/Habataki chromosome segment substitution lines (CSSLs, 39 accessions) were also used (Ando et al., 2008). Seeds were sterilized in 10% sodium hypochloric acid solution by vacuum infiltration for 1 h,and then immersed in aqueous 2% PPMTM solution (Nacalai Tesque, Kyoto, Japan, http://www.nacalai.co.jp/) at 28°C for 1 day in darkness. Seeds were sown in wet commercial fertilized soil (Bonsol II; Sumitomo Chemical, Tokyo, Japan, http://www.sumitomochem.co.jp/), and maintained under a 12-h light (28°C)/12-h dark(20°C) cycle for germination. Plants were kept under constant subirrigation conditions with tap water. After 2 weeks of growth, the entire aboveground (or aerial) part of one seedling was collected,weighed, and frozen in liquid nitrogen for analysis. Samples were |
| − | chromosome segment substitution lines (CSSLs, 39 accessions) were also used (Ando et al., 2008). Seeds were sterilized in 10% sodium hypochloric acid solution by vacuum infiltration for 1 h,and then immersed in aqueous 2% PPMTM solution (Nacalai Tesque, Kyoto, Japan, http://www.nacalai.co.jp/) at 28°C for 1 day in darkness. Seeds were sown in wet commercial fertilized soil (Bonsol II; Sumitomo Chemical, Tokyo, Japan, http://www.sumitomochem.co.jp/), and maintained under a 12-h light (28°C)/12-h dark(20°C) cycle for germination. Plants were kept under constant subirrigation conditions with tap water. After 2 weeks of growth, the entire aboveground (or aerial) part of one seedling was collected,weighed, and frozen in liquid nitrogen for analysis. Samples were | ||
stored at �80°C until analysis. | stored at �80°C until analysis. | ||
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Revision as of 02:17, 22 July 2016
Contents
Project Title
- Metabolome-genome-wide association study dissects genetic architecture for generating natural variation in rice secondary metabolism
The Background of This Project
Plant Culture & Treatment
- A Japanese rice collection of 175 accessions were used in this study (Table S1) (Yonemaru et al., 2012). The Sasanishiki/Habataki chromosome segment substitution lines (CSSLs, 39 accessions) were also used (Ando et al., 2008). Seeds were sterilized in 10% sodium hypochloric acid solution by vacuum infiltration for 1 h,and then immersed in aqueous 2% PPMTM solution (Nacalai Tesque, Kyoto, Japan, http://www.nacalai.co.jp/) at 28°C for 1 day in darkness. Seeds were sown in wet commercial fertilized soil (Bonsol II; Sumitomo Chemical, Tokyo, Japan, http://www.sumitomochem.co.jp/), and maintained under a 12-h light (28°C)/12-h dark(20°C) cycle for germination. Plants were kept under constant subirrigation conditions with tap water. After 2 weeks of growth, the entire aboveground (or aerial) part of one seedling was collected,weighed, and frozen in liquid nitrogen for analysis. Samples were
stored at �80°C until analysis.
Research Findings
Labs working on this Project
- RIKEN Center for Sustainable Resource Science, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, Japan,
- Department of Bioinformatic Engineering, Graduate School of Information Science and Technology, Osaka University, 1-5 Yamadaoka, Suita, Osaka, Japan,
- National Institute of Agrobiological Sciences, 2-1-2 Kannondai, Tsukuba, Ibaraki, Japan, and
- Graduate School of Pharmaceutical Sciences, Chiba University, Inohana 1-8-1, Chuo-ku, Chiba, Japan
Corresponding Author
- Kazuki Saito:ksaito@faculty.chiba-u.jp
