IC4R008-Phenomics-2015-26111541

Project Title

• Integrating Image-Based Phenomics and Association Analysis to Dissect the Genetic Architecture of Temporal Salinity Responses in Rice

The Background of This Project

• Salinity affects a significant portion of arable land and is particularly detrimental for irrigated agriculture, which provides onethird of the global food supply. Rice (Oryza sativa), the most important food crop, is salt sensitive. The genetic resources for salt tolerance in rice germplasm exist but are underutilized due to the difficulty in capturing the dynamic nature of physiological responses to salt stress. The genetic basis of these physiological responses is predicted to be polygenic.

Plant Culture & Treatment

• Seeds from 373 genotypes from the rice (Oryza sativa) diversity panel were surface sterilized with fungicide, thiram, and germinated on moist paper towels in plastic boxes for 3 d (Famoso et al., 2011; Zhao et al., 2011). Three uniformly germinated seeds of each genotype were transplanted to pots (150-mm diameter 3 200-mm height) filled with 2.6 kg of UC Mix and placed into square containers to allow for water to collect. Plants were thinned to one seedling per pot 6 d after transplanting (DAT). For the first 7 DAT, each pot was watered daily with approximately 150 mL from the top of the pot. Over the course of the three experiments, the greenhouse temperatures during the day averaged 28.8°C (62.02°C, SD) and 26.0°C (61.01°C, SD) at night. Relative

humidity was maintained at 63.4% (69.04%, SD) during the day and 69.7%(61.73%, SD) at night (Rotation Atomizer Defensor ABS3, Condair).

• Eight DAT, each pot was watered to a uniform weight so that approximately 600 mL of water was maintained in the soil. For the salt-stressed plants,100 mL of NaCl solution (270 mM NaCl:9.9 mM CaClb) was applied to the square dish, and small holes in the bottom of the pots allowed for the infiltration of salt into the soil through capillary action. Salt treatment was applied in two steps of 45 mM to reach a final concentration of 90 mM at 10 and 13 DAT(Supplemental Fig. S1A). Control plants received 100 mL of water on days 10 and 13 (Supplemental Fig. S2).

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Labs working on this Project

• Department of Agronomy and Horticulture (M.T.C., H.W.), Holland Computing Center (A.C.K.);
• Department of Statistics (D.W.), University of Nebraska, Lincoln, Nebraska 68583;
• The Plant Accelerator,Australian Plant Phenomics Facility, University of Adelaide, Urrbrae, South Australia 5064, Australia (B.B.);
• Phenomics and Bioinformatics Research Centre, University of South Australia, Adelaide, South Australia 5001, Australia (C.J.B.)

Corresponding Author

• Malachy T. Campbell:0000-0002-8257-3595;