Difference between revisions of "Os03g0264400"
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===Expression=== | ===Expression=== | ||
| − | *The expression level of OASA2 in plants homozygous and heterozygous for modified OASA2 was similar to that of nontransformants, suggesting that OASA2 transcription in GT plants was controlled in the same manner as endogenous OASA2, and that GTcould lead to a lower risk of gene silencing than in conventional overexpression approaches. | + | *The expression level of OASA2 in plants homozygous and heterozygous for modified OASA2 was similar to that of nontransformants, suggesting that OASA2 transcription in GT plants was controlled in the same manner as endogenous OASA2, and that GTcould lead to a lower risk of gene silencing than in conventional overexpression approaches.One potential problem for selecting GT products was that transcript levels of mutated OASA2 were expected to be relatively low in rice cells in which GT had occurred successfully because the mutated OASA2 is under the control of its own intrinsic promoter. Moreover, GT occurs at very low frequency compared to conventional transformation experiments.Thus, we optimized the concentration of 5MT required to select GT cells efficiently using a control vector containing a mutated OASA2 expression cassette driven by the intrinsic promoter and terminator (Fig. 2A); 500 mM 5MT was found to be optimal for selection of GT cells(Fig. 2B). |
===Evolution=== | ===Evolution=== | ||
Revision as of 10:37, 31 July 2016
OASA2—an a-subunit of anthranilate synthase that is a key enzyme of tryptophan (Trp)vbiosynthesis in rice (Oryza sativa)
Contents
Annotated Information
Function
- OASA2—an a-subunit of anthranilate synthase that is a key enzyme of tryptophan (Trp)vbiosynthesis in rice (Oryza sativa)
Expression
- The expression level of OASA2 in plants homozygous and heterozygous for modified OASA2 was similar to that of nontransformants, suggesting that OASA2 transcription in GT plants was controlled in the same manner as endogenous OASA2, and that GTcould lead to a lower risk of gene silencing than in conventional overexpression approaches.One potential problem for selecting GT products was that transcript levels of mutated OASA2 were expected to be relatively low in rice cells in which GT had occurred successfully because the mutated OASA2 is under the control of its own intrinsic promoter. Moreover, GT occurs at very low frequency compared to conventional transformation experiments.Thus, we optimized the concentration of 5MT required to select GT cells efficiently using a control vector containing a mutated OASA2 expression cassette driven by the intrinsic promoter and terminator (Fig. 2A); 500 mM 5MT was found to be optimal for selection of GT cells(Fig. 2B).
Evolution
Please input evolution information here.
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Labs working on this gene
- Plant Genome Engineering Research Unit, Agrogenomics Research Center, National Institute of Agrobiological Sciences, Tsukuba, Ibaraki 305–8602, Japan (H.S., H.O., S.T.);
- RIKEN Plant Science Center,Yokohama, Kanagawa 230–0045, Japan (A.O., F.M., K.S.); Graduate School of Pharmaceutical Sciences, Chiba University, Inage-ku, Chiba 263–8522, Japan (K.S.);
- Kihara Institute of Biological Research, Yokohama City University, Yokohama, Kanagawa 244–0813, Japan (S.T.)
References
- de Pater S, Neuteboom LW, Pinas JE, Hooykaas PJJ, van der Zaal BJ (2009) ZFN-induced mutagenesis and gene-targeting in Arabidopsis through Agrobacterium-mediated floral dip transformation. Plant Biotechnol J 7:821–835
