Difference between revisions of "Os03g0302900"
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===Function=== | ===Function=== | ||
===Mutation=== | ===Mutation=== | ||
| + | * Characterization of the ssd1 mutant. The ssd1 mutant was screened from the Tos17 mutant library, which is a mutant population induced by the Tos17 retrotransposon.23) In the progeny of heterozygous plants, the segregation ratio of the normal phenotype to the dwarf was 90:31, which corresponded to the expected 3:1 segregation ratio of a single recessive gene (�2 ¼ 0.003). The dwarf phenotype in rice is generally caused by a reduction in culm length. Based on the elongation pattern of internodes, rice dwarf mutants are classi�ed into six types: N-, dn-, dm-, d6-, nl-, and sh-type.24) Of these, the dn-type is de�ned by reduction in internodes length in the same proportion to the WT. ssd1 exhibited a reduction in the length of all internodes in the same proportion as in the WT, which is characteristic of the dn-type dwarf (Fig. 2A), with severe dwarf and wide, dark green leaves (Fig. 1A, B). Culm length of the mutant at harvest was about 17 cm, whereas the WT grew to about 90 cm (Figs. 1A, B and 2A). Elongation of the seminal and crown roots was also inhibited in the mutant (Figs. 1E and 2B), and the development of �oral organs was also impaired in ssd1. Rice �owers are composed of four kinds of glumes, two rudimentary glumes, two empty glumes, lemma and palea, and three kinds of �oral organs, two lodicules, six stamens, and one pistil.25) The ssd1 glumes were shorter than that of the WT (Fig. 1C, D). The ssd1 �owers also developed short anthers and �laments (Fig. 1F) and short, shrunken stigma (Fig. 1G). Some pistils developed three stigmas (Fig. 1G). These observations suggest that SSD1 has a fundamental role in cell division and/or elongation in various organs. | ||
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| + | [[File:Os03g0302900-1.png|center|thumb|400px|'''Fig. 1. Morphological characterization of the ssd1 mutant.''' '' <ref name="ref1" />.'']] | ||
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| + | [[File:Os03g0302900-2.png|center|thumb|400px|'''Fig. 2. Lengths of panicle, internodes, and seminal roots. (A) Diagram of internode lengths of Nipponbare and the ssd1 mutant. (B) Comparison of seminal root length between Nipponbare and the ssd1 mutant. �� indicates signi�cant di�erences at the 1% levels, as judged using the Student’s t test.''' '' <ref name="ref1" />.'']] | ||
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===Expression Pattern=== | ===Expression Pattern=== | ||
===Evolution=== | ===Evolution=== | ||
Revision as of 06:56, 1 August 2016
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Contents
Annotated Information
Function
Mutation
- Characterization of the ssd1 mutant. The ssd1 mutant was screened from the Tos17 mutant library, which is a mutant population induced by the Tos17 retrotransposon.23) In the progeny of heterozygous plants, the segregation ratio of the normal phenotype to the dwarf was 90:31, which corresponded to the expected 3:1 segregation ratio of a single recessive gene (�2 ¼ 0.003). The dwarf phenotype in rice is generally caused by a reduction in culm length. Based on the elongation pattern of internodes, rice dwarf mutants are classi�ed into six types: N-, dn-, dm-, d6-, nl-, and sh-type.24) Of these, the dn-type is de�ned by reduction in internodes length in the same proportion to the WT. ssd1 exhibited a reduction in the length of all internodes in the same proportion as in the WT, which is characteristic of the dn-type dwarf (Fig. 2A), with severe dwarf and wide, dark green leaves (Fig. 1A, B). Culm length of the mutant at harvest was about 17 cm, whereas the WT grew to about 90 cm (Figs. 1A, B and 2A). Elongation of the seminal and crown roots was also inhibited in the mutant (Figs. 1E and 2B), and the development of �oral organs was also impaired in ssd1. Rice �owers are composed of four kinds of glumes, two rudimentary glumes, two empty glumes, lemma and palea, and three kinds of �oral organs, two lodicules, six stamens, and one pistil.25) The ssd1 glumes were shorter than that of the WT (Fig. 1C, D). The ssd1 �owers also developed short anthers and �laments (Fig. 1F) and short, shrunken stigma (Fig. 1G). Some pistils developed three stigmas (Fig. 1G). These observations suggest that SSD1 has a fundamental role in cell division and/or elongation in various organs.
Fig. 1. Morphological characterization of the ssd1 mutant. [1].
Fig. 2. Lengths of panicle, internodes, and seminal roots. (A) Diagram of internode lengths of Nipponbare and the ssd1 mutant. (B) Comparison of seminal root length between Nipponbare and the ssd1 mutant. �� indicates signi�cant di�erences at the 1% levels, as judged using the Student’s t test. [1].
Expression Pattern
Evolution
Subcellular localization
Labs working on this gene
- Bioscience and Biotechnology Center, Nagoya University, Aichi, Japan.
- Division of Genome and Biodiversity Research, National Institute of Agrobiological Sciences, Ibaraki, Japan.
References
- ↑ 1.0 1.1 Asano K, Miyao A, Hirochika H, Kitano H, Matsuoka M, Ashikari M. SSD1, which encodes a plant-specific novel protein, controls plant elongation by regulating cell division in rice. Proc Jpn Acad Ser B Phys Biol Sci. 2010;86(3):265-73. PubMed PMID: 20228626; PubMed Central PMCID: PMC3417851.
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