Difference between revisions of "Os03g0320900"
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===Mutation=== | ===Mutation=== | ||
===Expression Pattern=== | ===Expression Pattern=== | ||
| − | * To examine stage-specific expression of the V2 gene during chloroplast and leaf development, | + | * To examine stage-specific expression of the V2 gene during chloroplast and leaf development, the researchers carried out RT-PCR analysis of various tissues of wild-type and v2 mutant seedlings that had been grown at the restrictive temperature until they had a fully emerged 3rd leaf. RT-PCR analysis revealed that, in the wild-type seedlings, V2 transcripts are much more abundant at an early stage of leaf development than at the later stages (Figure 1). In contrast, V2 transcripts were more abundant at later stages of leaf development in the v2 mutant. |
| − | [[File:.png|center|thumb|400px|'''Figure 1. RT-PCR analysis of V2 gene expression. Total RNA was extracted from seedlings of the wild-type and v2 mutant grown at the restrictive temperature ( | + | [[File:Os03g0320900-1.png|center|thumb|400px|'''Figure 1. RT-PCR analysis of V2 gene expression. Total RNA was extracted from seedlings of the wild-type and v2 mutant grown at the restrictive temperature (20℃) and amplified with gene-specific primers as described in Experimental procedures. The total RNA samples used as template for RT-PCR were run on a gel and stained with ethidium bromide (EtBr) to allow comparison of the sample concentrations (see bottom panel).''' '' <ref name="ref1" />.'']] |
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| + | * The researchers next examined the levels of V2 protein during leaf development by immunoblot analysis. The amounts of V2 protein in wild-type and v2 mutant seedlings paralleled those of V2 transcripts during chloroplast and leaf development (Figure 1,2). At the permissive temperature, the expression profiles of V2 protein in the v2 mutant were similar to those of the wild-type, although the expression levels of V2 protein in the aerial parts of the v2 mutant were higher (data not shown). Taken together, these results suggest that the V2 gene functions at an early stage of chloroplast and leaf development. | ||
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| + | [[File:Os03g0320900-2.png|center|thumb|400px|'''Figure 2. Immunoblot analysis of the V2 protein. Total soluble protein was extracted from wild-type and v2 mutant seedlings grown at the restrictive temperature (20℃). Equal amounts of total soluble protein (40 lg per lane) were loaded onto the gel. The V2 protein was detected using an anti-V2 protein antibody. Band ‘a’, at 27.5 kDa, corresponds to the V2 protein.''' '' <ref name="ref1" />.'']] | ||
===Evolution=== | ===Evolution=== | ||
Revision as of 08:20, 1 August 2016
- The rice gene Os03g0320900 was reported as v2 in 2007[1].
Contents
Annotated Information
Function
Mutation
Expression Pattern
- To examine stage-specific expression of the V2 gene during chloroplast and leaf development, the researchers carried out RT-PCR analysis of various tissues of wild-type and v2 mutant seedlings that had been grown at the restrictive temperature until they had a fully emerged 3rd leaf. RT-PCR analysis revealed that, in the wild-type seedlings, V2 transcripts are much more abundant at an early stage of leaf development than at the later stages (Figure 1). In contrast, V2 transcripts were more abundant at later stages of leaf development in the v2 mutant.
Figure 1. RT-PCR analysis of V2 gene expression. Total RNA was extracted from seedlings of the wild-type and v2 mutant grown at the restrictive temperature (20℃) and amplified with gene-specific primers as described in Experimental procedures. The total RNA samples used as template for RT-PCR were run on a gel and stained with ethidium bromide (EtBr) to allow comparison of the sample concentrations (see bottom panel). [1].
- The researchers next examined the levels of V2 protein during leaf development by immunoblot analysis. The amounts of V2 protein in wild-type and v2 mutant seedlings paralleled those of V2 transcripts during chloroplast and leaf development (Figure 1,2). At the permissive temperature, the expression profiles of V2 protein in the v2 mutant were similar to those of the wild-type, although the expression levels of V2 protein in the aerial parts of the v2 mutant were higher (data not shown). Taken together, these results suggest that the V2 gene functions at an early stage of chloroplast and leaf development.
Figure 2. Immunoblot analysis of the V2 protein. Total soluble protein was extracted from wild-type and v2 mutant seedlings grown at the restrictive temperature (20℃). Equal amounts of total soluble protein (40 lg per lane) were loaded onto the gel. The V2 protein was detected using an anti-V2 protein antibody. Band ‘a’, at 27.5 kDa, corresponds to the V2 protein. [1].
Evolution
Subcellular localization
Labs working on this gene
- Department of Biological Sciences, Faculty of Sciences, Kyushu University, Fukuoka 812-8581, Japan,
- Department of Biological Sciences, Graduate School of Science, University of Tokyo, Tokyo 113-0033, Japan,
- National Institute of Agrobiological Sciences, 2-1-2 Kannondai, Tsukuba 305-8602, Japan, and
- Plant Breeding Laboratory, Faculty of Agriculture, Kyushu University, Fukuoka 812-8581, Japan
References
Please input cited references here.
