Difference between revisions of "Os12g0601400"
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===Expression=== | ===Expression=== | ||
*The researchers performed semiquantitative reverse transcriptase–polymerase chain reaction (RT-PCR) analysis using primers that specifically amplified the OsIAA3 3¢ non-coding region. Wild-type(WT) plants were treated with media containing 20 lM IAA. The OsIAA3 transcript was increased by IAA within 15–30 min,and reached a maximum within 1 h (Figure 2a). | *The researchers performed semiquantitative reverse transcriptase–polymerase chain reaction (RT-PCR) analysis using primers that specifically amplified the OsIAA3 3¢ non-coding region. Wild-type(WT) plants were treated with media containing 20 lM IAA. The OsIAA3 transcript was increased by IAA within 15–30 min,and reached a maximum within 1 h (Figure 2a). | ||
| − | *To confirm that OsIAA3 also interacts with Aux/IAA and ARF, we performed a yeast two-hybrid assay using OsIAA3 and | + | *To confirm that OsIAA3 also interacts with Aux/IAA and ARF, we performed a yeast two-hybrid assay using OsIAA3 and OsARF1 (Sato et al., 2001; Waller et al., 2002). All transformed yeast cells formed colonies on the medium with histidine, while only yeasts expressing both OsIAA3 protein and OsIAA3 or OsIAA3 and OsARF1 protein formed colonies on the medium without histidine (Figure 2b). This indicates that OsIAA3 interacts with both OsIAA and OsARF proteins,as do Aux/IAA proteins in Arabidopsis. |
| − | OsARF1 (Sato et al., 2001; Waller et al., 2002). All transformed yeast cells formed colonies on the medium with histidine, while only yeasts expressing both OsIAA3 protein and OsIAA3 or OsIAA3 and OsARF1 protein formed colonies on the medium without histidine (Figure 2b). This indicates that OsIAA3 interacts with both OsIAA and OsARF proteins,as do Aux/IAA proteins in Arabidopsis. | + | *The researchers also analyzed the degradation of OsIAA3 protein produced in Escherichia coli with or without MG132 in the |
| + | crude extract of rice seedlings. The level of OsIAA3 protein decreased within 1 h without MG132 treatment, while it remained after 8 h of treatment with MG132 (Figure 2c). | ||
===Evolution=== | ===Evolution=== | ||
Revision as of 11:07, 7 August 2016
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Contents
Annotated Information
Function
- The IAA gene, a well-studied gene in Arabidopsis that serves as a negative regulator of auxin signaling.
- OsIAA3 is one of these family members whose expression is rapidly increased in response to auxin.
Expression
- The researchers performed semiquantitative reverse transcriptase–polymerase chain reaction (RT-PCR) analysis using primers that specifically amplified the OsIAA3 3¢ non-coding region. Wild-type(WT) plants were treated with media containing 20 lM IAA. The OsIAA3 transcript was increased by IAA within 15–30 min,and reached a maximum within 1 h (Figure 2a).
- To confirm that OsIAA3 also interacts with Aux/IAA and ARF, we performed a yeast two-hybrid assay using OsIAA3 and OsARF1 (Sato et al., 2001; Waller et al., 2002). All transformed yeast cells formed colonies on the medium with histidine, while only yeasts expressing both OsIAA3 protein and OsIAA3 or OsIAA3 and OsARF1 protein formed colonies on the medium without histidine (Figure 2b). This indicates that OsIAA3 interacts with both OsIAA and OsARF proteins,as do Aux/IAA proteins in Arabidopsis.
- The researchers also analyzed the degradation of OsIAA3 protein produced in Escherichia coli with or without MG132 in the
crude extract of rice seedlings. The level of OsIAA3 protein decreased within 1 h without MG132 treatment, while it remained after 8 h of treatment with MG132 (Figure 2c).
Evolution
Please input evolution information here.
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Labs working on this gene
- Bioscience and Biotechnology Center, Nagoya University, Chikusa, Nagoya, Aichi 464-8601, Japan
References
- Abel, S., Nguyen, M.D. and Theologis, A.(1995) The PS-IAA4/5-like family of early auxin-inducible messenger-RNAs in Arabidopsis thaliana. J. Mol. Biol. 251, 533–549.
