|
|
| (5 intermediate revisions by the same user not shown) |
| Line 1: |
Line 1: |
| − | '''''lpa N15-186''''' is a mutant allele of the rice myo-inositol kinase (OsMIK) gene<ref name="ref1" />.
| |
| | | | |
| − | ==Annotated Information==
| |
| − | ===Function===
| |
| − | Please input function information here.
| |
| − |
| |
| − | ===Mutation===
| |
| − |
| |
| − | * In order to conWrm that these two mutants are lpa mutants, the seed total P, phytic acid-P (PA-P) and inorganic P (Pi) contents were examined (Table 1).
| |
| − |
| |
| − | [[File:Os03g0737701-1.png|center|thumb|400px| '''Table 1 Comparison of seed myo-inositol, phytic acid and inorganic P contents of lpa mutants and wild-types.''' '' <ref name="ref1" />.'']]
| |
| − |
| |
| − | * Seed extracts of lpa mutants were subjected to HPLC analysis to determine if the other inositol phosphates were aVected by the mutation. Comparison to wild-type Nipponbare and inositol phosphate standards revealed that N15- 186 seeds are signiWcantly reduced in PA and inositol monophosphate while exhibiting an increase in Pi (Fig. 1). No signiWcant accumulation of other inositol phosphate intermediates (InsP2 to InsP5) was detected under the conditions used.
| |
| − |
| |
| − | [[File:Os03g0737701-2.png|center|thumb|400px| '''Fig. 1 HPLC chromatograms of inositol phosphates. a Inositol phosphate standards, retention times for the various inositol phosphates were: Ins(2)P1, 9.5 min; Pi, 15.5 min, Ins(1,4)P2, 21 min; Ins(1,3,4)P3, 32 min; Ins(13,4,5)P4, 33 min; Ins(1,3,4,5,6)P5, 35 min; and Ins(1,2,3,4,5,6)P6, 29 min. b Nipponbare wild-type, c N15-186 lpa mutant.''' '' <ref name="ref1" />.'']]
| |
| − |
| |
| − | * The GC–MS analysis revealed that the myo-inositol content,
| |
| − | as well as those of other sugars (glucose, fructose and
| |
| − | galactose), was increased in the N15-186 mutant compared
| |
| − | to the wild-type (Fig. 2).
| |
| − |
| |
| − | [[File:Os03g0737701-3.png|center|thumb|400px|'''Fig. 2 GC–MS analysis of myoinositol and other carbohydrates as alditol hexaacetate derivatives from seed extracts of wild-type, N-186 and N-375 mutants.''' '' <ref name="ref1" />.'']]
| |
| − |
| |
| − | ===Expression===
| |
| − | * RT-PCR analysis of total
| |
| − | RNA from various tissues including shoot, root, and panicle
| |
| − | indicated that OsMIK is expressed in all tissues (Fig. 6).
| |
| − | Higher expression was detected in the vegetative shoot tissue
| |
| − | compared to the reproductive tissue with relatively
| |
| − | lower expression in the roots. A search of publicly available
| |
| − | rice EST data indicated that OsMIK is expressed in callus,
| |
| − | leaf, root, shoot and panicle tissues (http://www.tigr.org/).
| |
| − |
| |
| − | ===Evolution===
| |
| − | Please input evolution information here.
| |
| − |
| |
| − | You can also add sub-section(s) at will.
| |
| − |
| |
| − | ==Labs working on this gene==
| |
| − | Please input related labs here.
| |
| − |
| |
| − | ==References==
| |
| − | <references>
| |
| − | * <ref name="ref1">
| |
| − | Kim S I, Andaya C B, Newman J W, et al. Isolation and characterization of a low phytic acid rice mutant reveals a mutation in the rice orthologue of maize MIK[J]. Theoretical and applied genetics, 2008, 117(8): 1291-1301.
| |
| − | </ref>
| |
| − | * <ref name="ref2">
| |
| − |
| |
| − | </ref>
| |
| − | * <ref name="ref3">
| |
| − |
| |
| − | </ref>
| |
| − | </references>
| |
| − |
| |
| − | ==Structured Information==
| |
| − | [[Category:Genes]][[Category:Oryza Sativa Japonica Group]][[Category:Japonica Chromosome 03]]
| |
