Difference between revisions of "Os03g0821300"
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===Mutation=== | ===Mutation=== | ||
| − | * To explore the role of Xb15 in resistance, the researchers examined whether constitutive overexpression of Xb15 would affect resistance to Xoo. The resulting transgenic plants (NTAP-XB15, pollen donor) were crossed with another Kitaake line possessing an N-terminal Myc-epitope-tagged XA21, under control of its native promoter (Myc-XA21, pollen recipient). The presence | + | * To explore the role of Xb15 in resistance, the researchers examined whether constitutive overexpression of Xb15 would affect resistance to Xoo. The resulting transgenic plants (NTAP-XB15, pollen donor) were crossed with another Kitaake line possessing an N-terminal Myc-epitope-tagged XA21, under control of its native promoter (Myc-XA21, pollen recipient). The presence of the Myc-Xa21 and/or Ntap-Xb15 gene in the resulting F1 was confirmed by the PCR analysis (unpublished data). Next, they tested the expression of NTAP-XB15 and Myc-XA21 in the cross (XA21/XB15 17A-21 and 19A-72) using an anti-Myc antibody and peroxidase-anti-peroxidase (PAP, for detecting the TAP tag) (Figure 3A). Six-week-old progeny from self-pollinated XA21/XB15 17A-18, 17A-21, 19A-64, and 19A-72 were then inoculated with Xoo PR6 strain PXO99 expressing AvrXA21 and examined for cosegregation of genotype with phenotype by PCR analysis and measurement of the length of Xoo-induced lesions. All Myc-Xa21 plants overexpressing Ntap-Xb15 (Myc-Xa21/Ntap- Xb15, +/+) by PCR analysis showed accumulation of NTAPXB15 by protein gel blot analysis and displayed enhanced susceptibility to Xoo PR6 compared to transgenic plants carrying Myc-Xa21 alone (+/-) (Figure 3B). Figure 3C shows a picture of two typical leaves from each of the following inoculated rice plants: F2 segregants of XA21/ XB15 17A and 19A, and transgenic plants overexpressing NTAP only (NTAP) 16 d after Xoo PR6 inoculation. |
| − | of the Myc-Xa21 and/or Ntap-Xb15 gene in the resulting F1 was confirmed by the PCR analysis (unpublished data). Next, they tested the expression of NTAP-XB15 and Myc-XA21 in the cross (XA21/XB15 17A-21 and 19A-72) using an anti-Myc antibody and peroxidase-anti-peroxidase (PAP, for detecting the TAP tag) (Figure 3A). Six-week-old progeny from self-pollinated XA21/XB15 17A-18, 17A-21, 19A-64, and 19A-72 were then inoculated with Xoo PR6 strain PXO99 expressing AvrXA21 and examined for cosegregation of genotype with phenotype by PCR analysis and measurement of the length of Xoo-induced lesions. All Myc-Xa21 plants overexpressing Ntap-Xb15 (Myc-Xa21/Ntap- Xb15, | ||
===Expression=== | ===Expression=== | ||
Revision as of 13:50, 6 September 2016
Xb15 encodes a PP2C that negatively regulates the XA21-mediated innate immune response.
Contents
Annotated Information
Function
- The rice XA21 protein is representative of the large class of non-RD RKs predicted to be involved in plant innate immunity. XB15 encoded by Xb15 Is an XA21 Binding Protein.
Mutation
- To explore the role of Xb15 in resistance, the researchers examined whether constitutive overexpression of Xb15 would affect resistance to Xoo. The resulting transgenic plants (NTAP-XB15, pollen donor) were crossed with another Kitaake line possessing an N-terminal Myc-epitope-tagged XA21, under control of its native promoter (Myc-XA21, pollen recipient). The presence of the Myc-Xa21 and/or Ntap-Xb15 gene in the resulting F1 was confirmed by the PCR analysis (unpublished data). Next, they tested the expression of NTAP-XB15 and Myc-XA21 in the cross (XA21/XB15 17A-21 and 19A-72) using an anti-Myc antibody and peroxidase-anti-peroxidase (PAP, for detecting the TAP tag) (Figure 3A). Six-week-old progeny from self-pollinated XA21/XB15 17A-18, 17A-21, 19A-64, and 19A-72 were then inoculated with Xoo PR6 strain PXO99 expressing AvrXA21 and examined for cosegregation of genotype with phenotype by PCR analysis and measurement of the length of Xoo-induced lesions. All Myc-Xa21 plants overexpressing Ntap-Xb15 (Myc-Xa21/Ntap- Xb15, +/+) by PCR analysis showed accumulation of NTAPXB15 by protein gel blot analysis and displayed enhanced susceptibility to Xoo PR6 compared to transgenic plants carrying Myc-Xa21 alone (+/-) (Figure 3B). Figure 3C shows a picture of two typical leaves from each of the following inoculated rice plants: F2 segregants of XA21/ XB15 17A and 19A, and transgenic plants overexpressing NTAP only (NTAP) 16 d after Xoo PR6 inoculation.
Expression
Please input expression information here.
Evolution
- The researchers next compared the PP2C domain of XB15 with other evolutionary related proteins from the reference plant species rice and Arabidopsis, such as Arabidopsis POL and POL-like proteins (PLLs) (Figure 2).
Figure 2. Phylogenetic Relationships among POL and PLL Genes from Rice and Arabidopsis [1].
Labs working on this gene
Please input related labs here.
References
- ↑ Park CJ, Peng Y, Chen X, Dardick C, Ruan D, Bart R, Canlas PE, Ronald PC. Rice XB15, a protein phosphatase 2C, negatively regulates cell death and XA21-mediated innate immunity. PLoS Biol. 2008 Sep 23;6(9):e231. doi: 10.1371/journal.pbio.0060231. Erratum in: PLoS Biol. 2008 Nov;6(11):e282. PubMed PMID: 18817453; PubMed Central PMCID: PMC2553837.
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