Difference between revisions of "Os04g0390500"
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| − | * To investigate the role of '''''OsYSL6''''', the researchers obtained a Tos- 17 insertion line (NC6189; osysl6). No transcript of '''''OsYSL6''''' was detected in this line, indicating that this is a knockout line of '''''OsYSL6'''''. When the plants were subjected to different Mn concentrations, the growth of osysl6 did not differ from the wild-type rice at Mn concentrations up to 100 mM but was reduced at 1,000 mM (Fig. 1). At 1,000 mM Mn treatment, the fresh weight of the shoots and roots in the knockout line was 58% and 53%, respectively, of the wild type (Fig. 2). | + | * To investigate the role of '''''OsYSL6''''', the researchers obtained a Tos- 17 insertion line (NC6189; osysl6). No transcript of '''''OsYSL6''''' was detected in this line, indicating that this is a knockout line of '''''OsYSL6'''''. When the plants were subjected to different Mn concentrations, the growth of osysl6 did not differ from the wild-type rice at Mn concentrations up to 100 mM but was reduced at 1,000 mM (Fig. 1). At 1,000 mM Mn treatment, the fresh weight of the shoots and roots in the knockout line was 58% and 53%, respectively, of the wild type (Fig. 2). The older true leaf of the knockout line exhibited necrosis, |
| + | a typical Mn toxicity symptom(Fig. 1F), which was not observed in the wild-type rice (Fig. 1E). | ||
[[File:Os04g0390500-1.png|center|thumb|400px|'''Figure 1. Phenotypic analysis of the OsYSL6 knockout line in response to Mn. Both wild-type rice (left) and knockout line osysl6 (right) were grown in a nutrient solution containing 0.05 (A), 0.5 (B), 100 (C), or 1,000 (D) mM MnCl2 for 3 weeks. E and F show close-up views of the oldest true leaves of wild-type rice (E) and osysl6 (F) exposed to 1,000 mM MnCl2 for 3 weeks. Photographs were taken at harvest.''' '' <ref name="ref1" />.'']] | [[File:Os04g0390500-1.png|center|thumb|400px|'''Figure 1. Phenotypic analysis of the OsYSL6 knockout line in response to Mn. Both wild-type rice (left) and knockout line osysl6 (right) were grown in a nutrient solution containing 0.05 (A), 0.5 (B), 100 (C), or 1,000 (D) mM MnCl2 for 3 weeks. E and F show close-up views of the oldest true leaves of wild-type rice (E) and osysl6 (F) exposed to 1,000 mM MnCl2 for 3 weeks. Photographs were taken at harvest.''' '' <ref name="ref1" />.'']] | ||
Revision as of 11:30, 11 September 2016
Yellow Stripe-Like (YSL) proteins belong to the oligopeptide transporter family and have been implicated in metal transport and homeostasis in different plant species. OsYSL6 is one of the rice (Oryza sativa) YSL members .
Contents
Annotated Information
Function
Please input function information here.
Mutation
- To investigate the role of OsYSL6, the researchers obtained a Tos- 17 insertion line (NC6189; osysl6). No transcript of OsYSL6 was detected in this line, indicating that this is a knockout line of OsYSL6. When the plants were subjected to different Mn concentrations, the growth of osysl6 did not differ from the wild-type rice at Mn concentrations up to 100 mM but was reduced at 1,000 mM (Fig. 1). At 1,000 mM Mn treatment, the fresh weight of the shoots and roots in the knockout line was 58% and 53%, respectively, of the wild type (Fig. 2). The older true leaf of the knockout line exhibited necrosis,
a typical Mn toxicity symptom(Fig. 1F), which was not observed in the wild-type rice (Fig. 1E).
Figure 1. Phenotypic analysis of the OsYSL6 knockout line in response to Mn. Both wild-type rice (left) and knockout line osysl6 (right) were grown in a nutrient solution containing 0.05 (A), 0.5 (B), 100 (C), or 1,000 (D) mM MnCl2 for 3 weeks. E and F show close-up views of the oldest true leaves of wild-type rice (E) and osysl6 (F) exposed to 1,000 mM MnCl2 for 3 weeks. Photographs were taken at harvest. [1].
Figure 2. Effects of different Mn concentrations on the growth of the OsYSL6 knockout line. Both wild-type rice (WT) and the knockout line (osysl6) were cultivated in a nutrient solution containing 0.05, 0.5, 100, or 1,000 mM MnCl2. After 3 weeks, the shoots (A) and roots (B) were harvested and their fresh weights were recorded. Data are means of three biological replicates. An asterisk above a bar indicates a significant difference (P , 0.05) between the wild type and the knockout line by Tukey’s test. [1].
Expression
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Evolution
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Labs working on this gene
- Institute of Plant Science and Resources, Okayama University, Kurashiki 710–0046, Japan
References
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