Difference between revisions of "Os04g0396500"
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===Mutation=== | ===Mutation=== | ||
| − | * lax2 mutations were isolated while screening for mutants with abnormal panicle development. The researchers found three independent mutant alleles, all of which exhibited a sparse-panicle phenotype. These alleles, recovered from ethyl methanesulfonate, tissue culture, and g-ray irradiation mutagenesis populations, were designated as lax2-1, lax2-2, and lax2-3, respectively. | + | * lax2 mutations were isolated while screening for mutants with abnormal panicle development. The researchers found three independent mutant alleles, all of which exhibited a sparse-panicle phenotype. These alleles, recovered from ethyl methanesulfonate, tissue culture, and g-ray irradiation mutagenesis populations, were designated as lax2-1, lax2-2, and lax2-3, respectively. To characterize the nature of the sparse appearance of the lax2-1 panicle, the researchers counted the number of inflorescence branches in the wild type and mutant. The lax2-1 mutant panicle produced fewer spikelets compared with the wild-type panicle (Figures 1A and 1E). Quantitative analysis of the lax2-1 mutant panicle revealed that the number of PBs was not affected in the mutant (Figure 1F). However, the number of total lateral branches, which was the sum of the number of PBs, SBs, and spikelets in a panicle, was reduced in lax2-1 (Figure 1G). This indicates that the sparse panicle appearance and the reduction in spikelet number in the lax2-1 mutant are attributable both to reduced higher order branching, such as the number of SBs, and to a reduced number of spikelets borne directly on branches (Figure 1B). Because a spikelet other than a terminal spikelet is produced as a lateral branch from an inflorescence meristem, lax2-1 mutants seem to have a defect in producing all the lateral branches other than PBs in panicles. lax2-1 mutants also had defects in vegetative development. In lax2-1 mutants, the number of tillers produced during vegetative growth was reduced (Figures 1C and 1H). Because there was no trace of tiller buds at the base of the leaves in lax2-1 mutants (Figure 1D), the reduction in the number of tillers is caused by a defect in AM formation and not by enhanced apical dominance or growth arrest of tiller buds. As a result of the reduction in the number of tillers in lax2-1, mature lax2-1 plants had fewer panicles than wild-type plants. Thus, the lax2-1 mutant failed to develop lateral branches during both vegetative and reproductive development, although the number of PBs in the panicle was not affected. |
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===Expression=== | ===Expression=== | ||
Revision as of 13:03, 11 September 2016
Please input one-sentence summary here.
Contents
Annotated Information
Function
- LAX2 encodes a nuclear protein with a plant-specific conserved domain and that LAX2 physically interacts with LAX1 through a region containing this conserved domain. Thus, the rsearchers propose that LAX2 is a novel genetic component that is required for the process of AM formation and acts together with LAX1 in rice.
Mutation
- lax2 mutations were isolated while screening for mutants with abnormal panicle development. The researchers found three independent mutant alleles, all of which exhibited a sparse-panicle phenotype. These alleles, recovered from ethyl methanesulfonate, tissue culture, and g-ray irradiation mutagenesis populations, were designated as lax2-1, lax2-2, and lax2-3, respectively. To characterize the nature of the sparse appearance of the lax2-1 panicle, the researchers counted the number of inflorescence branches in the wild type and mutant. The lax2-1 mutant panicle produced fewer spikelets compared with the wild-type panicle (Figures 1A and 1E). Quantitative analysis of the lax2-1 mutant panicle revealed that the number of PBs was not affected in the mutant (Figure 1F). However, the number of total lateral branches, which was the sum of the number of PBs, SBs, and spikelets in a panicle, was reduced in lax2-1 (Figure 1G). This indicates that the sparse panicle appearance and the reduction in spikelet number in the lax2-1 mutant are attributable both to reduced higher order branching, such as the number of SBs, and to a reduced number of spikelets borne directly on branches (Figure 1B). Because a spikelet other than a terminal spikelet is produced as a lateral branch from an inflorescence meristem, lax2-1 mutants seem to have a defect in producing all the lateral branches other than PBs in panicles. lax2-1 mutants also had defects in vegetative development. In lax2-1 mutants, the number of tillers produced during vegetative growth was reduced (Figures 1C and 1H). Because there was no trace of tiller buds at the base of the leaves in lax2-1 mutants (Figure 1D), the reduction in the number of tillers is caused by a defect in AM formation and not by enhanced apical dominance or growth arrest of tiller buds. As a result of the reduction in the number of tillers in lax2-1, mature lax2-1 plants had fewer panicles than wild-type plants. Thus, the lax2-1 mutant failed to develop lateral branches during both vegetative and reproductive development, although the number of PBs in the panicle was not affected.
Expression
Please input expression information here.
Evolution
Please input evolution information here.
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Labs working on this gene
- Crop Development Division, National Agriculture and Food Research Organization Agricultural Research Center, Niigata 943-0193, Japan
- National Center for Plant Gene Research, Key Laboratory of Molecular and Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, People’s Republic of China
- Department of Biological Mechanisms and Functions, Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya 464-8601, Japan
- Photosynthesis and Photobiology Research Unit, National Institute of Agrobiological Sciences, Tsukuba 305-8602, Japan
- State Key Laboratory of Rice Biology, China National Rice Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310006, People’s Republic of China
- Institute of Radiation Breeding, National Institute of Agrobiological Sciences, Hitachi-ohmiya 319-2293, Japan
- Bioscience and Biotechnology Center, Nagoya University, Nagoya 464-8601, Japan
- Rice Research Division, National Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan
- Department of Agricultural and Environmental biology, Graduate School of Agricultural and Life Sciences, University of Tokyo, Tokyo 113-8657, Japan
- Precursory Research for Embryonic Science and Technology, Japan Science and Technology Agency, Kawaguchi 332-0012, Japan
References
- ↑ Tabuchi H, Zhang Y, Hattori S, Omae M, Shimizu-Sato S, Oikawa T, Qian Q, Nishimura M, Kitano H, Xie H, Fang X, Yoshida H, Kyozuka J, Chen F, Sato Y. LAX PANICLE2 of rice encodes a novel nuclear protein and regulates the formation of axillary meristems. Plant Cell. 2011 Sep;23(9):3276-87. doi: 10.1105/tpc.111.088765. Epub 2011 Sep 30. PubMed PMID: 21963665; PubMed Central PMCID: PMC3203427.
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