Difference between revisions of "Os08g0131100"
(→Phenotypic analysis) |
(→Phenotypic analysis) |
||
| Line 8: | Line 8: | ||
* CYP703A3 represents a conserved and diversified biochemical pathway for in‐chain hydroxylation of lauric acid required for the development of male organ in higher plants. | * CYP703A3 represents a conserved and diversified biochemical pathway for in‐chain hydroxylation of lauric acid required for the development of male organ in higher plants. | ||
===Phenotypic analysis=== | ===Phenotypic analysis=== | ||
| − | * There were no detectable differences during the early stages of the anther development between the cyp703a3-2 and wild-type plants. Until stage 9 of anther development, the | + | * Even though cyp703a3‐2 has normal vegetative development, and inflorescence and floral morphology (Figure 1A–D), its anthers appeared pale yellow and smaller without mature pollen grains compared with the wild type (Figure 1E–I). |
| − | * At stage 10, wild‐type microspores became round and vacuolated with thick surrounding exine, and the tapetum layer was thin because of tapetal degradation (Figure 2C), while | + | * There were no detectable differences during the early stages of the anther development between the cyp703a3-2 and wild-type plants. Until stage 9 of anther development, the cyp703a3-2 mutant could undergo normal meiosis and released normal young microspores similar to the wild type (Figure 2A, B, F and G). |
| + | * At stage 10, wild‐type microspores became round and vacuolated with thick surrounding exine, and the tapetum layer was thin because of tapetal degradation (Figure 2C), while cyp703a3-2 micro- spores had thin and weakly stained exine, indicating abnormal sporopollinin deposition (Figure 2H). | ||
* At stage 11, the uninucleate microspore of wild‐type plants underwent the first mitotic division, generating binucleate pollen grains (Figure 2D) (Zhang and Wilson 2009). However, at this stage, cyp703a3‐2 microspores developed irregular and disintegrating appearance, becoming aggregated at one side of the anther locule (Figure 2I). | * At stage 11, the uninucleate microspore of wild‐type plants underwent the first mitotic division, generating binucleate pollen grains (Figure 2D) (Zhang and Wilson 2009). However, at this stage, cyp703a3‐2 microspores developed irregular and disintegrating appearance, becoming aggregated at one side of the anther locule (Figure 2I). | ||
* At stage 13, the wild‐type anther locule was full of mature pollen grains, by contrast, the mutant anther locule was empty and flat (Figure 2E and J). | * At stage 13, the wild‐type anther locule was full of mature pollen grains, by contrast, the mutant anther locule was empty and flat (Figure 2E and J). | ||
Revision as of 06:40, 3 October 2016
The rice Os08g0131100 was reported as CYP703A3 (genomes uncoupled 4) in 2014 [1] by researchers from China.
Contents
Annotated Information
Gene Symbol
- Os08g0131100 <=> CYP703A3,OsCYP703A3
Function
- The Oryza sativa mutant cyp703a3‐2 caused by one base insertion in CYP703A3 displays defective pollen exine and anther epicuticular layer, which differs from Arabidopsis cyp703a2 in which only defective pollen exine occurs.
- CYP703A3 represents a conserved and diversified biochemical pathway for in‐chain hydroxylation of lauric acid required for the development of male organ in higher plants.
Phenotypic analysis
- Even though cyp703a3‐2 has normal vegetative development, and inflorescence and floral morphology (Figure 1A–D), its anthers appeared pale yellow and smaller without mature pollen grains compared with the wild type (Figure 1E–I).
- There were no detectable differences during the early stages of the anther development between the cyp703a3-2 and wild-type plants. Until stage 9 of anther development, the cyp703a3-2 mutant could undergo normal meiosis and released normal young microspores similar to the wild type (Figure 2A, B, F and G).
- At stage 10, wild‐type microspores became round and vacuolated with thick surrounding exine, and the tapetum layer was thin because of tapetal degradation (Figure 2C), while cyp703a3-2 micro- spores had thin and weakly stained exine, indicating abnormal sporopollinin deposition (Figure 2H).
- At stage 11, the uninucleate microspore of wild‐type plants underwent the first mitotic division, generating binucleate pollen grains (Figure 2D) (Zhang and Wilson 2009). However, at this stage, cyp703a3‐2 microspores developed irregular and disintegrating appearance, becoming aggregated at one side of the anther locule (Figure 2I).
- At stage 13, the wild‐type anther locule was full of mature pollen grains, by contrast, the mutant anther locule was empty and flat (Figure 2E and J).
Expression
Please input expression information here.
Evolution
Please input evolution information here.
You can also add sub-section(s) at will.
Labs working on this gene
Please input related labs here.
References
Please input cited references here.
Structured Information
- ↑ Cite error: Invalid
<ref>tag; no text was provided for refs namedref1
