Difference between revisions of "Os02g0649300"
(→References) |
(→Expression) |
||
| Line 14: | Line 14: | ||
* Expressing preferentially in the root throughout the whole developmental stages, '''''OsEXPA3''''' displayed the expression peak in 1-wk-old seedlings but decreased drastically at the tillering stage, and the expression diminution continued with the passage of growth time. | * Expressing preferentially in the root throughout the whole developmental stages, '''''OsEXPA3''''' displayed the expression peak in 1-wk-old seedlings but decreased drastically at the tillering stage, and the expression diminution continued with the passage of growth time. | ||
* As to abiotic stresses and nutrient deficiency, '''''OsEXPA3''''' expression in the root was significantly upregulated by NaCl stress, while repressed by N and Pi deficiency. The result implies an intrinsic function of '''''OsEXPA3''''' in regulation of the root system architecture in response to abiotic stresses. | * As to abiotic stresses and nutrient deficiency, '''''OsEXPA3''''' expression in the root was significantly upregulated by NaCl stress, while repressed by N and Pi deficiency. The result implies an intrinsic function of '''''OsEXPA3''''' in regulation of the root system architecture in response to abiotic stresses. | ||
| − | + | [[File:Os02g0649300-1|center|thumb|800px|'''Figure 3.''' ''Effects of OsEXPA3 downregulation on the root system architecture. (a) Primary root length of 1-wk-old seedlings. (b) Lateralroot density of 1-wk-old seedlings. Two independent transgenic lines (Line 1 and Line 3) were analyzed. The seeds were placed on half-strength Murashige and Skoog solid medium and grown for 7 d vertically in a growth chamber. Primary root length was determined using a ruler. All visible lateral roots that emerged from the primary root were counted. Values are the means of 20 biological replications | |
| + | ± standard error. One independent plant was considered as one biological replication.<ref name="ref1" />.'']] | ||
You can also add sub-section(s) at will. | You can also add sub-section(s) at will. | ||
Revision as of 11:14, 3 October 2016
The rice Os02g0649300 was reported as OsEXPA3 in 2014 [1] by researchers from China.
Contents
Annotated Information
Gene Symbol
- Os02g0649300 <=> OsEXPA3
Function
- OsEXPA3 is required for root system development in rice and involved in mediating root growth by mediating cell-wall loosening
- OsEXPA3 is a critical root expansin gene that is intrinsically involved in root system architecture adapted to some abiotic stresses.
- Modification of cell-wall compositions mediated by OsEXPA3 repression changed the wall mechanical properties, which elucidated the cell loosening process both biochemically and mechanically.
Expression
- Semiquantitative-PCR analysis demonstrated that OsEXPA3 expressed preferentially in the root of 1-wk- old seedlings.
- Expressing preferentially in the root throughout the whole developmental stages, OsEXPA3 displayed the expression peak in 1-wk-old seedlings but decreased drastically at the tillering stage, and the expression diminution continued with the passage of growth time.
- As to abiotic stresses and nutrient deficiency, OsEXPA3 expression in the root was significantly upregulated by NaCl stress, while repressed by N and Pi deficiency. The result implies an intrinsic function of OsEXPA3 in regulation of the root system architecture in response to abiotic stresses.
File:Os02g0649300-1
Figure 3. Effects of OsEXPA3 downregulation on the root system architecture. (a) Primary root length of 1-wk-old seedlings. (b) Lateralroot density of 1-wk-old seedlings. Two independent transgenic lines (Line 1 and Line 3) were analyzed. The seeds were placed on half-strength Murashige and Skoog solid medium and grown for 7 d vertically in a growth chamber. Primary root length was determined using a ruler. All visible lateral roots that emerged from the primary root were counted. Values are the means of 20 biological replications ± standard error. One independent plant was considered as one biological replication.[1].
You can also add sub-section(s) at will.
Labs working on this gene
- Key Lab. of Biorheological Science and Technology, Ministry of Education, Bioengineering College, Chongqing Univ., Chongqing, 400030, P. R. China.
