Difference between revisions of "Os02g0649300"
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===Expression=== | ===Expression=== | ||
| − | * Quantitative RT-PCR result showed that | + | * Quantitative RT-PCR result showed that in the seedlings, '''''OsSLI1''''' expression was mainly in root. |
* The root tissue accumulates 7 times of '''''OsSLI1''''' transcripts than in shoot tissue. | * The root tissue accumulates 7 times of '''''OsSLI1''''' transcripts than in shoot tissue. | ||
| − | * In panicles, the expression changes in different development stages, from relative low in 3 cm young panicles to a 33-fold increase in 8 cm panicles, and the 12 cm panicles still accumulate 8-fold OsSLI1 transcripts compared to 3 cm young panicles tissue | + | * In panicles, the expression changes in different development stages, from relative low in 3 cm young panicles to a 33-fold increase in 8 cm panicles, and the 12 cm panicles still accumulate 8-fold OsSLI1 transcripts compared to 3 cm young panicles tissue. |
| − | + | * Quantitative RT-PCR assay showed that OsSLI1 expression was highly induced by salt, PEG, H2O2 , high temperature, and ABA treatment but not by cold treatment. | |
| + | ===Subcellular Localization=== | ||
| + | * '''''OsSLI1''''' is a nuclear-localized protein, in consistency with its potential function as a DNA binding transcription factor. | ||
[[File:6-Os02g0649300-1.png|center|thumb|800px|'''Figure 3.''' ''Effects of OsEXPA3 downregulation on the root system architecture. (a) Primary root length of 1-wk-old seedlings. (b) Lateralroot density of 1-wk-old seedlings. Two independent transgenic lines (Line 1 and Line 3) were analyzed. The seeds were placed on half-strength Murashige and Skoog solid medium and grown for 7 d vertically in a growth chamber. Primary root length was determined using a ruler. All visible lateral roots that emerged from the primary root were counted. Values are the means of 20 biological replications | [[File:6-Os02g0649300-1.png|center|thumb|800px|'''Figure 3.''' ''Effects of OsEXPA3 downregulation on the root system architecture. (a) Primary root length of 1-wk-old seedlings. (b) Lateralroot density of 1-wk-old seedlings. Two independent transgenic lines (Line 1 and Line 3) were analyzed. The seeds were placed on half-strength Murashige and Skoog solid medium and grown for 7 d vertically in a growth chamber. Primary root length was determined using a ruler. All visible lateral roots that emerged from the primary root were counted. Values are the means of 20 biological replications | ||
± standard error. One independent plant was considered as one biological replication.<ref name="ref1" />.'']] | ± standard error. One independent plant was considered as one biological replication.<ref name="ref1" />.'']] | ||
Revision as of 11:30, 3 October 2016
The rice Os02g0649300 was reported as OsSLI1 in 2014 [1] by researchers from China.
Contents
Annotated Information
Gene Symbol
- Os02g0649300 <=> OsSLI1
Function
- OsSLI1 encodes member of the HD-Zip I subfamily
- OsSLI1 may be a transcriptional activator regulating stress-responsive gene expression and panicle development in rice.
Expression
- Quantitative RT-PCR result showed that in the seedlings, OsSLI1 expression was mainly in root.
- The root tissue accumulates 7 times of OsSLI1 transcripts than in shoot tissue.
- In panicles, the expression changes in different development stages, from relative low in 3 cm young panicles to a 33-fold increase in 8 cm panicles, and the 12 cm panicles still accumulate 8-fold OsSLI1 transcripts compared to 3 cm young panicles tissue.
- Quantitative RT-PCR assay showed that OsSLI1 expression was highly induced by salt, PEG, H2O2 , high temperature, and ABA treatment but not by cold treatment.
Subcellular Localization
- OsSLI1 is a nuclear-localized protein, in consistency with its potential function as a DNA binding transcription factor.
Figure 3. Effects of OsEXPA3 downregulation on the root system architecture. (a) Primary root length of 1-wk-old seedlings. (b) Lateralroot density of 1-wk-old seedlings. Two independent transgenic lines (Line 1 and Line 3) were analyzed. The seeds were placed on half-strength Murashige and Skoog solid medium and grown for 7 d vertically in a growth chamber. Primary root length was determined using a ruler. All visible lateral roots that emerged from the primary root were counted. Values are the means of 20 biological replications ± standard error. One independent plant was considered as one biological replication.[1].
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Labs working on this gene
- State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China
