Difference between revisions of "Os02g0649300"

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The rice '''''Os02g0649300''''' was reported as '''''OsEXPA3''''' in 2014 <ref name="ref1" /> by researchers from China.  
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The rice '''''Os02g0649300''''' was reported as '''''OsSLI1''''' in 2014 <ref name="ref1" /> by researchers from China.  
  
 
==Annotated Information==
 
==Annotated Information==
 
===Gene Symbol===
 
===Gene Symbol===
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*'''''Os02g0649300''''' '''''<=>''''' '''''OsEXPA3'''''
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*'''''Os02g0649300''''' '''''<=>''''' '''''OsSLI1'''''
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[[File:7-Os02g0649300-1.png|right|thumb|357px|'''Figure 6.''' ''Nuclear localization of OsSLI1-GFP fusion protein.<ref name="ref1" />.'']]
 
===Function===
 
===Function===
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* '''''OsEXPA3''''' is required for root system development in rice and involved in mediating root growth by mediating cell-wall loosening
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* '''''OsSLI1''''' encodes member of the HD-Zip I subfamily
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* '''''OsEXPA3''''' is a critical root expansin gene that is intrinsically involved in root system architecture adapted to some abiotic stresses.
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* '''''OsSLI1''''' may be a transcriptional activator regulating stress-responsive gene expression and panicle development in rice.
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* Modification of cell-wall compositions mediated by '''''OsEXPA3''''' repression changed the wall mechanical properties, which elucidated the cell loosening process both biochemically and mechanically.
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* It was found that OsSLI1 expression was enhanced in an ABI5-Like1 (ABL1) deficiency mutant abl1 under both normal and stress conditions, suggesting that ABL1 probably negatively regulates '''''OsSLI1''''' gene expression.
  
 
===Expression===
 
===Expression===
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* Semiquantitative-PCR analysis demonstrated that '''''OsEXPA3''''' expressed preferentially in the root of 1-wk- old seedlings.
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* Quantitative RT-PCR result showed that in the seedlings, '''''OsSLI1''''' expression was mainly in root.  
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* Expressing preferentially in the root throughout the whole developmental stages, '''''OsEXPA3''''' displayed the expression peak in 1-wk-old seedlings but decreased drastically at the tillering stage, and the expression diminution continued with the passage of growth time.
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* The root tissue accumulates 7 times of '''''OsSLI1''''' transcripts than in shoot tissue.
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* As to abiotic stresses and nutrient deficiency, '''''OsEXPA3''''' expression in the root was significantly upregulated by NaCl stress, while repressed by N and Pi deficiency. The result implies an intrinsic function of '''''OsEXPA3''''' in regulation of the root system architecture in response to abiotic stresses.
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* In panicles, the expression changes in different development stages, from relative low in 3 cm young panicles to a 33-fold increase in 8 cm panicles, and the 12 cm panicles still accumulate 8-fold OsSLI1 transcripts compared to 3 cm young panicles tissue.
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[[File:Os02g0649300-1.png|center|thumb|800px|'''Figure 3.''' ''Effects of OsEXPA3 downregulation on the root system architecture. (a) Primary root length of 1-wk-old seedlings. (b) Lateralroot density of 1-wk-old seedlings. Two independent transgenic lines (Line 1 and Line 3) were analyzed. The seeds were placed on half-strength Murashige and Skoog solid medium and grown for 7 d vertically in a growth chamber. Primary root length was determined using a ruler. All visible lateral roots that emerged from the primary root were counted. Values are the means of 20 biological replications
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* Quantitative RT-PCR assay showed that OsSLI1 expression was highly induced by salt, PEG, H2O2 , high temperature, and ABA treatment but not by cold treatment.
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± standard error. One independent plant was considered as one biological replication.<ref name="ref1" />.'']]
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===Subcellular Localization===
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* '''''OsSLI1''''' is a nuclear-localized protein, in consistency with its potential function as a DNA binding transcription factor.
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You can also add sub-section(s) at will.
 
You can also add sub-section(s) at will.
  
 
==Labs working on this gene==
 
==Labs working on this gene==
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* Key Lab. of Biorheological Science and Technology, Ministry of Education, Bioengineering College, Chongqing Univ., Chongqing, 400030, P. R. China.
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* State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China
  
 
==References==
 
==References==
 
<references>
 
<references>
 
* <ref name="ref1">
 
* <ref name="ref1">
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Qiu, Shichun, et al. "Repression of Expression Leads to Root System Growth Suppression in Rice." Crop Science 54.5 (2014): 2201-2213.
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Huang X, Duan M, Liao J, Yuan X, Chen H, Feng J, Huang J, Zhang HS. OsSLI1, a  homeodomain containing transcription activator, involves abscisic acid related stress response in rice (Oryza sativa L.). ScientificWorldJournal. 2014;2014:809353. doi: 10.1155/2014/809353. Epub 2014 Jun 25. PubMed PMID: 25089296; PubMed Central PMCID: PMC4095735.
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</ref>
 
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</references>
 
</references>
  
 
==Structured Information==
 
==Structured Information==
 
     [[Category:Genes]][[Category:Oryza Sativa Japonica Group]][[Category:Japonica Chromosome 2]]
 
     [[Category:Genes]][[Category:Oryza Sativa Japonica Group]][[Category:Japonica Chromosome 2]]

Latest revision as of 11:34, 3 October 2016

The rice Os02g0649300 was reported as OsSLI1 in 2014 [1] by researchers from China.

Annotated Information

Gene Symbol

  • Os02g0649300 <=> OsSLI1
Figure 6. Nuclear localization of OsSLI1-GFP fusion protein.[1].

Function

  • OsSLI1 encodes member of the HD-Zip I subfamily
  • OsSLI1 may be a transcriptional activator regulating stress-responsive gene expression and panicle development in rice.
  • It was found that OsSLI1 expression was enhanced in an ABI5-Like1 (ABL1) deficiency mutant abl1 under both normal and stress conditions, suggesting that ABL1 probably negatively regulates OsSLI1 gene expression.

Expression

  • Quantitative RT-PCR result showed that in the seedlings, OsSLI1 expression was mainly in root.
  • The root tissue accumulates 7 times of OsSLI1 transcripts than in shoot tissue.
  • In panicles, the expression changes in different development stages, from relative low in 3 cm young panicles to a 33-fold increase in 8 cm panicles, and the 12 cm panicles still accumulate 8-fold OsSLI1 transcripts compared to 3 cm young panicles tissue.
  • Quantitative RT-PCR assay showed that OsSLI1 expression was highly induced by salt, PEG, H2O2 , high temperature, and ABA treatment but not by cold treatment.

Subcellular Localization

  • OsSLI1 is a nuclear-localized protein, in consistency with its potential function as a DNA binding transcription factor.


You can also add sub-section(s) at will.

Labs working on this gene

  • State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China

References

  1. ↑ 1.0 1.1 Cite error: Invalid <ref> tag; no text was provided for refs named ref1

Structured Information

Retrieved from "https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os02g0649300&oldid=273522"