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| − | Please input one-sentence summary here.
| + | The rice '''''Os07g0409900''''' was reported as '''''CPK18''''' in 2014 <ref name="ref1" /> by researchers from China. |
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| | ==Annotated Information== | | ==Annotated Information== |
| | + | |
| | + | [[File:11-Os07g0409900.png|right|thumb|527px|'''Figure 7.''' ''A Working Model for the CPK18-MPK5 Pathway.<ref name="ref1" />.'']] |
| | + | ===Gene Symbol=== |
| | + | *'''''Os07g0409900''''' '''<=>''' '''''CPK18''''','''''OsCPK18''''' |
| | + | |
| | ===Function=== | | ===Function=== |
| − | Please input function information here.
| + | * '''''OsCPK18''''' is a rice (Oryza sativa) calcium-dependent protein kinase (CDPK), it is as an upstream kinase of MAPK (MPK5) in vitro and in vivo. |
| | + | * '''''OsCPK18''''' was shown to phosphorylate and activate MPK5 without affecting the phosphorylation of its TXY motif. Instead, CPK18 was found to predominantly phosphorylate two Thr residues (Thr-14 and Thr-32) that are widely conserved in MAPKs from land plants. |
| | + | * Further analyses reveal that the newly identified CPK18-MPK5 pathway represses defense gene expression and negatively regulates rice blast resistance. |
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| − | ===Expression=== | + | ===CPK18-MPK5 Pathway Model=== |
| − | Please input expression information here.
| + | * Plasma membrane–associated CPK18 is regulated by Ca 2+ signals to activate MPK5 through Thr-14/Thr-32 phosphorylation (pT14/pT32). Activated MPK5 then phosphorylates different transcription factors to activate or repress downstream gene expression. |
| | + | * The CPK18-MPK5 pathway may work independently or cooperatively with the canonical MAPK cascade, which is activated by pathogen-associated molecular pattern (PAMP) and pattern recognition receptor (PRR) interaction. |
| | + | * MPK5 likely exists in multiple phosphorylated forms with variable activities. Arrows indicate positive regulation; the blunt-ended line indicates negative regulation. Dashed arrows indicate undefined pathways of MPK5 feedback phosphorylation of CPK18. |
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| − | ===Evolution=== | + | ===Subcellular Localization=== |
| − | Please input evolution information here.
| + | * Transient expression of green fluorescent protein (GFP)–tagged MPK5 and CPK18 in onion (Allium cepa) epidermal cells and rice protoplasts showed that MPK5 was localized in the nucleus and cytoplasm, whereas '''''CPK18''''' was predominantly associated with the plasma membrane. |
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| | You can also add sub-section(s) at will. | | You can also add sub-section(s) at will. |
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| | ==Labs working on this gene== | | ==Labs working on this gene== |
| − | Please input related labs here.
| + | * Department of Plant Pathology and Environmental Microbiology, The Huck Institutes of the Life Sciences, Pennsylvania State University, University Park, Pennsylvania 16802 |
| | | | |
| | ==References== | | ==References== |
| − | Please input cited references here.
| + | <references> |
| | + | * <ref name="ref1"> |
| | + | Xie K, Chen J, Wang Q, Yang Y. Direct phosphorylation and activation of amitogen-activated protein kinase by a calcium-dependent protein kinase in rice. Plant Cell. 2014 Jul;26(7):3077-89. doi: 10.1105/tpc.114.126441. Epub 2014 Jul 17. PubMed PMID: 25035404; PubMed Central PMCID: PMC4145133. |
| | + | </references> |
| | | | |
| | ==Structured Information== | | ==Structured Information== |
| − | {{JaponicaGene|
| + | [[Category:Genes]][[Category:Oryza Sativa Japonica Group]][[Category:Japonica Chromosome 7]] |
| − | GeneName = Os07g0409900|
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| − | Description = Protein kinase-like domain containing protein|
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| − | Version = NM_001065979.1 GI:115471690 GeneID:4343002|
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| − | Length = 8523 bp|
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| − | Definition = Oryza sativa Japonica Group Os07g0409900, complete gene.|
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| − | Source = Oryza sativa Japonica Group
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| − | | |
| − | ORGANISM Oryza sativa Japonica Group
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| − | Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
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| − | Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
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| − | clade; Ehrhartoideae; Oryzeae; Oryza.
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| − | |
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| − | Chromosome = [[:category:Japonica Chromosome 7|Chromosome 7]]|
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| − | AP = Chromosome 7:13455198..13463720|
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| − | CDS = 13455398..13455553,13455717..13455890,13456607..13456669,13457844..13457951,13458040..13458155<br>,13458925..13459051,13460160..13460329,13461146..13461224,13461295..13461406<br>,13461801..13461842,13462273..13462403,13463294..13463554|
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| − | GCID = <gbrowseImage1>
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| − | name=NC_008400:13455198..13463720
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| − | source=RiceChromosome07
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| − | preset=GeneLocation
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| − | </gbrowseImage1>|
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| − | GSID = <gbrowseImage2>
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| − | name=NC_008400:13455198..13463720
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| − | source=RiceChromosome07
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| − | preset=GeneLocation
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| − | </gbrowseImage2>|
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| − | CDNA = <cdnaseq>atgggactctgctcctcctccagcgcccgccgggacgccggcacacccggcggcggtaacggcgcggggaacaaggataacgcggggaggaaggggatcgtggcgtgcgggaagcggacggacttcgggtacgacaaggacttcgaggcgcggtacgcgctcgggaagctgctgggccacggccagttcggctacaccttcgccgccgtcgaccgccgctccagcgagcgcgtcgccgtcaagcgcatcgacaagaacaagatggttcttcctgttgccgttgaagacgtaaagcgagaagttaaaatactgaaggccttacaaggccatgaaaatgttgtacatttttacaatgcatttgaagatgataattatgtgtatattgttatggaattatgtgaaggcggggagttacttgaccggatactagccaagaaagatagccgttatagcgagaaagatgctgcagtagttgtgcggcaaatgctcaaggttgctgctgagtgccatttgcatggtttggttcatcgggacatgaagcctgagaacttcctcttcaaatcaaccaaagaggactcatccctcaaggctacagattttggtctttcagattttataagaccagggaaacactttcgtgacattgttggaagtgcctactatgtagcaccagaagtgcttaagcgtaagtcaggcccagaatctgacgtttggagtattggcgtaataacctatattctactgtgtggaagacgacctttctgggacaaaactgaagatggaatatttaaagaggtgttgaaaaacaagccagattttcgtcgcaagccctggccaaatatcactccttgtgctaaagactttgtacaaaagttgcttgttaaggatccccgtgcaagattaactgctgctcaggcattatcacatgaatgggtgagagaaggaggacaggcatctgatatacctctagatatatctgtattacataatatgcgtcagtttgtaaaatacagtcggtttaagcaatttgctttacgggcgttagcttctacactaaatgcagaagagttgtctgatcttcgtgaccagttcaatgccattgatgttgacaagaatggaacaattagtctggaagaactgaagcaggctcttgcaaaggatgttccatggagattaaagggtccacgtgttttagagattgttgaggcaattgacagtaacacagatggattagttgatttcgaagagtttgttgctgcaacattacatgtgcatcagctagtggaacatgatactgagaagtggaaatcattgtctcaagctgcatttgataaatttgatgttgacggagatggctatatcacatctgatgaactgagaatgcaaacaggactgaaaggttctattgatcccctcctggaggaggctgacattgacagagatggaaaaataagcctagatgaatttcgcaggctcctgaaaactgcaagcatgagttcacgcaatgtacaaactccgaggagtgttcacagatcgtag</cdnaseq>|
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| − | AA = <aaseq>MGLCSSSSARRDAGTPGGGNGAGNKDNAGRKGIVACGKRTDFGY DKDFEARYALGKLLGHGQFGYTFAAVDRRSSERVAVKRIDKNKMVLPVAVEDVKREVK ILKALQGHENVVHFYNAFEDDNYVYIVMELCEGGELLDRILAKKDSRYSEKDAAVVVR QMLKVAAECHLHGLVHRDMKPENFLFKSTKEDSSLKATDFGLSDFIRPGKHFRDIVGS AYYVAPEVLKRKSGPESDVWSIGVITYILLCGRRPFWDKTEDGIFKEVLKNKPDFRRK PWPNITPCAKDFVQKLLVKDPRARLTAAQALSHEWVREGGQASDIPLDISVLHNMRQF VKYSRFKQFALRALASTLNAEELSDLRDQFNAIDVDKNGTISLEELKQALAKDVPWRL KGPRVLEIVEAIDSNTDGLVDFEEFVAATLHVHQLVEHDTEKWKSLSQAAFDKFDVDG DGYITSDELRMQTGLKGSIDPLLEEADIDRDGKISLDEFRRLLKTASMSSRNVQTPRS VHRS</aaseq>|
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| − | DNA = <dnaseqindica>8168..8323#7831..8004#7052..7114#5770..5877#5566..5681#4670..4796#3392..3561#2497..2575#2315..2426#1879..1920#1318..1448#167..427#ggtgaggtctcctcactcctctcctctctctctctctgctctgctttgaccccaattcccaacacccctcccccttcgcttcgctgctcccgattccggcagcctcgcgccgccgccacattgatggtaatagttggatccgccgcctccgccgcagccgacgacgatgggactctgctcctcctccagcgcccgccgggacgccggcacacccggcggcggtaacggcgcggggaacaaggataacgcggggaggaaggggatcgtggcgtgcgggaagcggacggacttcgggtacgacaaggacttcgaggcgcggtacgcgctcgggaagctgctgggccacggccagttcggctacaccttcgccgccgtcgaccgccgctccagcgagcgcgtcgccgtcaagcgcatcgacaagaacaaggtatgggcccaaacgagcgtggtggtgtgcggctgacgcgtgggggccatgtgcccatgtgttgctcgattcgatcggccagttgggctagcctggacgacgaccacctttgcggggggcgtcgcgttttgcacgtggggttgggttgccgaggaaatgtgaaatgtgaatccggggcgatactgtagtggtagtggtacaaatcaaaaatgcactgccactacctaatgctgctgcatgttagtactagcgcaacttgcagattgccatggaggtgatccatctaaaccatcgaatctggtgggatgactccattcctcatttcttcactcagtatatgtacttttcagggatgaggtccattcctcaaaccaaggaaactattttggatcaagtggtcaatggacatctcaatcctcaaaccaaaataccaaactattttgggatgtttaaatcttcattcttgagcaaaataggaaccagtcaggatggaggcaacaagtggataatggactatacacatacgtaatggacacatgtggcgtatacaaatttgtttagttccaaaaccagtataaagactgcacaaatgcctcctaacagatacggcaaggcaattcctgaaagacactgatatgtactgaaacgtaacacattcgtatatgaccctacttctcctgtacatgaaggtcataaattcatgagcagagaaatgtaatatgtgtattcagaaaataggacgcactgattttctggactctgcattattgtaaagccttgtcagcatgtgctatgctgaattgaagcatcaacagaagtagagaactagcctctattttgaaaagaaatgatactgctctaacttctcaaatgggatgttttcgttgcagatggttcttcctgttgccgttgaagacgtaaagcgagaagttaaaatactgaaggccttacaaggccatgaaaatgttgtacatttttacaatgcatttgaagatgataattatgtgtatattgttatggagtaagtcatggaaaaactctcaaatgtttatgaaatcagtagaagtatatctaagtataaccataaaatgtctaagtgaacttttcatggccttagccaagttgaaggaaggttcatatgtgtgtttatgcttttttttagaaaccacccatattttaagtaattgatcttactgaggtctagtattttagtttgtgcccctccaaacaggactttggcaattcaggatctaattacatggaaaagaaacaccacatgcaaactggcataatattaataccctcgtaaaaatctagaaattgtaccgggtaaatatcaagttcaggataaatagttggcaaacactttcatgtttctcatctttgttctctttgtatcaaggaactgtgcaagctaatgaagatctgaccctgttacatgtatttgacagattatgtgaaggcggggagttacttgaccggatactagccaagtaagtcaatttttgaagtttgtcaaaaatttttactttaccacagaactgtggcatgcttttttggttgtgttgggattacttattctaaatatagggatgttgaaccggagagcatttcaattagtgcttcgtgccacaaagagcttggaatttaatatggatgtatggcatatagtcatatattgttttcctattaccataaaaccaaaatattacagggattgacataagtttatgtgagacatccactagccatcaactgtcaggaattgttcaaagattttttttcgactgaatgttcaaagatttttatttattattaagttcgtttgaatatttgatgtatgtagaaagcaaattcactgatatcacatttgttctgctcttacaggaaagatagccgttatagcgagaaagatgctgcagtagttgtgcggcaaatgctcaaggttgctgctgagtgccatttgcatggtttggttcatcgggacatgaagcctgaggtatgctctaaaagtacaagactaagtcttcttataagagtaattgatcatttgatatgtgtatttacagaacttcctcttcaaatcaaccaaagaggactcatccctcaaggctacagattttggtctttcagattttataagaccaggtttgtgtgtatgttgtaactacttgttctattgttttcttgtttaactttcttccattccatatgaaatataaaagagaaaaatctctatttggccttgtaagttagccatatcccttatttgctctcccgaaactaaacctttttacccttctagtaattcctattctattagaggtatggcttattatattttctatgttaatcccttctaattccttgtcaatgtaaaaagggcattcaccgatccatgtgcataaaaaaatgttgataattataagtagtagtaaaacttgatcgccatcttttattagaacatacattctttttagagaaccaagtctgcaggcatagaaaatgacaaaactatttacaaattttagtagcagacatagaaaaaggtatttaggcccgagccaattaaaaagaaaaacagctattttactatgcatttataaaaaaactaacatgctaaagttatactctctacatgttaaaaagatattggttttttttattaatgacaacattagaaataggaagactaaaaaagaagagataattgtccttgcttccagttgagaaggcattagaaggtaaaaaagtacttcacacataattcaagtagcaaactaatggagaagtaatacgaggggcaaatatagaaacaaagttgagaaagatgggcatgaggggttgagcagttccgcatgggcaaatgaaggatacgactaacttttgagggcaaatataaagaattttctcaataaagaaattattctcagtgtaattgaaattaagttgccgcactcacagggaaacactttcgtgacattgttggaagtgcctactatgtagcaccagaagtgcttaagcgtaagtcaggcccagaatctgacgtttggagtattggcgtaataacctatattctactgtgtggaagacgacctttctgggacaaaactgaagatggaatatttaaagaggtcattttgcctaaactatctttgtcttacaaccattttattacatgtgtttcattgattaaaactattttcttgccttacaatcactatattttcttctttttttccttcgatgttgccgtattatccgtattactttgatatcgttctaacttttagtagtccatacttccaaggaagtgcgcatgttattcttaaaatacttctggtctgttttaaggaaattcagaatcttaggctggtatgtttcagttaagtggctcttcatcttgtccaaaaaggaagaggaaggatcagcacatgggtctagatgatgtggcaaatgacatggcaaaacagaatgatgtggaaagccacctcacttaaaaccacttgagaaacaatcatagggacattatttgcatgatttcaattgttgagtgacaagttgtcttagagttgacttgagagacaaaaattgacctacatgaagagttgacagaccaaagatagactttccccttttttagataaaaaagactaactctttatcttctcaggatttatgctcaagtatatctgatctagtgatctgtgtcatgacaaatctgaaaaatatgatccaacttgttgaaaagatgaaaataccttgctatgcagtgcacttcatagcatacgagtttgccattggcacaatatcaggcgtgtagatttacactgattatatacttgggaaatagcttaccacaagcgtcaacaagtttatatgctatccagaaaagcacgcatccatggatgaaccgagtgaactatcttagaacaagtatcgtaatgtgtacaaatgctagcataggaggatctatgtggagtcaatctacatgattcaccctctctccttacctaaacctatatggagctaagcctgcagtctatggaacattagctgtgcctgtgcataaagaaaaatatgtatatcttgggtagccgctaccctggtggctgcttatatttatgtttctctttgggtatcatgttggaattactgaccttgtctcaaatcgtgtccatcctctgttccccgcttctctttccacaatgctaacaaagtggcatattctttttggtgcaggtgttgaaaaacaagccagattttcgtcgcaagccctggccaaatatcactccttgtgctaaagactttgtacaaaagttgcttgttaaggatccccgtgcaagattaactgctgctcaggcattatgtatgtaactttatatcatttttggatgtcttaaatttaagtatgataatctgattagggaaactctatatcattttgggacatcttgaatgcaagtttgcaaatctggttatttaataatgggattctagtggcaaactgttgctaagtctgagtgcagtcatgcaaattcacaacttgattactgggtgactcttagatgggatatatctaaggggatttatgaagggccccttcaggttggaaacttatgtatgagggtccaacctcaaaggcttactcagcgatcattaatgggtttgcagtgcatactccctgttagcttaatacttatttgtttaacttctgctgggggttcagctttggcttgttatttgaagccatgtggctatgcttgctactcatgaaattcagacctccattgccttttatcgatttgattgttaagttattgctatgtatatggtagcactttctgattaccatgcgtaaaataacagcttataccttgaatttgatagaaacatcttatgttgttttttcatggttagacatggttggttcagttactaaggcttggtacaatctaatattgatacataaccaataccaactaattcatgttttcattacatcatagcaactaacatttgctgttcagttggttatttatcagttatcacagatggcatactgttggtataaaaataaacaatcttattatcacagtgctctactaacatgaaaatttacatattattttcttcagcacatgaatgggtgagagaaggaggacaggcatctgatatacctctagatatatctgtattacataatatgcgtcagtttgtaaaatacagtcggtttaagcaatttgctttacgggtaagttatcataccataacttaagggaatcttatccatgactcaggatgatttcggagtcaattttaaatgtttacatctcttgtaggcgttagcttctacactaaatgcagaagagttgtctgatcttcgtgaccagttcaatgccattgatgttgacaagaatggaacaattagtctggaagaactgaagcaggtacatccttgttggaattcctcgctacactattggcttgtttttttttactaatatttagcataatccatggaatacatttctttgtaatttctcttcatcttatttttgggccactgttctgaccaagctctcttccattctgccacttccctcatgccaacattctgtccgtttggaaacaaatactggaaatgtagaaaaatgaaaatagggaaaaccataggataatgtaagaagtatataatggcaaaactgtttagggattgccattgttaaattgtacaaaccccagtggctgatgcaggattttggagatgggggtacaaacttgtaaagaaacattaatacatggaaaaacctaaagctccctattaaagcaaataatactcaattattcagtaaatcagaaaataagaaaaaatatttataaatcctttgaaaaaataaatattaaggtttcagcacaagcttatatataagttggctggaactcttttggggctattttgcaactatggaatcaaatactgctgttctaactcaacaaatcatatacacatgcattctaactattgaatgaaagggtgtttacaaaatccatttacatagttatgtcccatgctggtccaccatgacaaagcatcagatttttcttaaaattttacttttgtacatttctcaagaaatcacaccacaaaaaatgtttctgttttctcaattattctgaactattctaaaattttcaattggtaattgtttgtaagtcaggtagatttaatgatatcttctgttaagtgctagagattataactactatttgttactctccctagtgaaagatgtgcgataaagctcttttgttcattaaggaaagcatcctgatgaccacagctgcaccatcaaagttcttttggtagtcattgatgcatagacattttgattatgaaattttctttttaacttgtattcaaagaattggaaacatgcttctttttctttgaagaagtaccatatatttgctcttatacacatatcaaagaaacaaattctatgttgtaccttctcttatacatatgacatgcggaacttcggcagtgtcaattttttacctcacattataagtcatctgtcttattagctatcactatatgatgtcaacactaatttgcaggctcttgcaaaggatgttccatggagattaaagggtccacgtgttttagagattgttgaggcagtatgttctcattcttataatttaccgtagtaacattatctattatcggtctcagattcacatgctttcttgtggtcacttggttggaactatgtgcaattgcaatgaagtgttaaatctttctaaaacatttaatgacgttatgcaaatagtttgcattttcaaaatttcactgtcccatccttgacagcttttgagagtgaagtcatcttccatgttttagtatatagtaatagtatttttccaaggcagctagggtaagagatgataaaaattatatggatttcatatattttattatttagtgctgaagatatagaagattggagtagttgtatacttattctagctatttcgagatgatgtggggggttttaggtcaaatgagttaagatgcctaaatggtactgtttaataatagataacagtattgaaactgagtaaatatattcctctctgcctttttgttctttgactaagcaaatgcatccacttccctctctatgactcttatgacaatgcttgatgttgttttactgatgatcctcaaatatagctttgtagtaagttttaaattatagttccagatgcaatgtttcaagttccattgtttctattaaatttgcatgtatgcaattgaacggacatcatcaagttttctccttgcaaaggggagtatttgctatcatggcataaagattttcattgctgggcagattgacagtaacacagatggattagttgatttcgaagagtttgttgctgcaacattacatgtgcatcagctagtggaacatgatactgagaagtggaaatcattgtctcaagctgcatttgataaatttgatgttgacggagatggctatatcacatctgatgaactgagaatggtacacctcttcaaatacctgtgaaacattactcttttcctattcacgtgagaattgatttatttcctataagattccaatccttaattggtgcatagcataagatcgtaatttattcaaccagttcatgcttctcatatatgtattcttacattgtctgcagcaaacaggactgaaaggttctattgatcccctcctggaggaggctgacattgacagagatggaaaaataagcctagatgaatttcgcaggctcctgaaaactgcaagcatgagttcacgcaatgtacaaactccgaggagtgttcacagatcgtagcttttcatagctgtgcacacttcacaaaagtgaacaaagggcagtcattgttgtaatatttttcatcgacacgtgtaatcaaaattgttgccagaaaagctcttgatgtaaattaatatggacatgtaggactgaagctagccagctaatctgtagatgtgcctcgagaaattaaacgttgtcttgcatacgtttgtgtg</dnaseqindica>|
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| − | Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001065979.1 RefSeq:Os07g0409900]|
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| − | }}
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| − | [[Category:Genes]] | |
| − | [[Category:Japonica mRNA]]
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| − | [[Category:Oryza Sativa Japonica Group]] | |
| − | [[Category:Japonica Genes]] | |
| − | [[Category:Japonica Chromosome 7]]
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| − | [[Category:Chromosome 7]]
| |
