Difference between revisions of "Os02g0101500"

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Please input one-sentence summary here.
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The rice '''''Os02g0101500''''' was reported as '''''OsHPR1''''' in 2014 <ref name="ref1" /> by researchers from China.  
  
 
==Annotated Information==
 
==Annotated Information==
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[[File:Os02g0101500.png|right|thumb|327px|'''Figure 4.''' ''Subcellular localizations of GFP–HPR1 and GFP-HPR2 fusions in transgenic rice protoplasts.<ref name="ref1" />.'']]
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===Gene Symbol===
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*'''''Os02g0101500''''' '''<=>''' '''''OsHPR1''''','''''HPR1'''''
 +
 
===Function===
 
===Function===
Please input function information here.
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* The researchers constructed over-expression of the '''''OsHPR1''''' gene and RNA interference plants of '''''OsHPR1''''' and '''''OsHPR2''''' genes in rice.
 +
* The phenotypes of all transgenic lines were determined in ambient air and CO 2 -elevated air. The phenotype typical of photorespiration mutants were observed only where activity of both '''''HPR1''''' and '''''HPR2''''' were down-regulated in the same line.
 +
* Double deletion of '''''OsHPR1''''' and '''''OsHPR2''''' genes significantly reduces plant growth under normal photorespiration condition
 +
* These findings demonstrate that two hydroxypyruvate reductases encoded by OsHPR1 and OsHPR2 are involved in photorespiratory metabolism in rice.
  
 
===Expression===
 
===Expression===
Please input expression information here.
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* Results from RT-PCR, western-blot and enzyme assays showed that '''''HPR1''''' activity changed significantly in corresponding transgenic lines without any effect on '''''HPR2''''' activity, which is the same for '''''HPR2'''''.
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 +
===Subcellular Localization===
 +
* In the present study, the subcellular localization of '''''OsHPR1''''' protein was first predicted to be located in the peroxisome and cytosol respectively.
 +
* In agreement with the prediction of HPR1 localization, the spots of HPR1-GFP and PTS1-CFP fusions were quite similar in the co-transformation protoplast, indicating the subcellular localization of HPR1 protein is in the peroxisome.
  
===Evolution===
 
Please input evolution information here.
 
  
 
You can also add sub-section(s) at will.
 
You can also add sub-section(s) at will.
  
 
==Labs working on this gene==
 
==Labs working on this gene==
Please input related labs here.
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* State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, South China Agricultural University, Guangzhou, China,
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* The Chinese University of Hong Kong Shenzhen Institute, Shenzhen, China,
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* State Key Laboratory of Agrobiotechnology, The Chinese University of Hong Kong, Hong Kong, China
  
 
==References==
 
==References==
Please input cited references here.
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<references>
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* <ref name="ref1">
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Ye N, Yang G, Chen Y, Zhang C, Zhang J, Peng X. Two hydroxypyruvate reductases encoded by OsHPR1 and OsHPR2 are involved in photorespiratory metabolism in rice. J Integr Plant Biol. 2014 Feb;56(2):170-80. doi: 10.1111/jipb.12125. Epub 2014 Jan 8. PubMed PMID: 24401104.
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</ref>
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</references>
  
 
==Structured Information==
 
==Structured Information==
{{JaponicaGene|
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    [[Category:Genes]][[Category:Oryza Sativa Japonica Group]][[Category:Japonica Chromosome 2]]
GeneName = Os02g0101500|
 
Description = Similar to NADH-dependent hydroxypyruvate reductase (EC 1.1.1.29) (Fragment)|
 
Version = NM_001052124.1 GI:115443618 GeneID:4327981|
 
Length = 4398 bp|
 
Definition = Oryza sativa Japonica Group Os02g0101500, complete gene.|
 
Source = Oryza sativa Japonica Group
 
 
 
  ORGANISM  Oryza sativa Japonica Group
 
            Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
 
            Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
 
            clade; Ehrhartoideae; Oryzeae; Oryza.
 
|
 
Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|
 
AP = Chromosome 2:78483..82880|
 
CDS = 80082..80104,80194..80308,80480..80524,80726..80911,81027..81101<br>,81213..81304,81731..81803,81923..82004,82096..82217<br>,82300..82440,82509..82592,82682..82804|
 
GCID = <gbrowseImage1>
 
name=NC_008395:78483..82880
 
source=RiceChromosome02
 
preset=GeneLocation
 
</gbrowseImage1>|
 
GSID = <gbrowseImage2>
 
name=NC_008395:78483..82880
 
source=RiceChromosome02
 
preset=GeneLocation
 
</gbrowseImage2>|
 
CDNA = <cdnaseq>atggcgaagccgatatcgatcgaggtgtggaatccgagcggaaagtatcgcgtggtgagcaccaagtccatgcccggcacccgctggatccgcctcctcgccgacaacgactgccgcctcgagatctgcacggagacgaagaccatcctctctgtcgacgacatcctcgccctcattggcgatcgctgcgatggtgtcatcggccagctgacggaggagtggggagaggtgctgttctcggcgctgaagcgcgccggcggaacagccttcagcaacatggccgtcgggtacaacaacgtcgacgtggaggccgccaataggaacggcatcgccgtcggcaacacgcctggcgttctgacggagacgacggcggagctggccgcgtctctgtcgctggcggcggcgcggaggatcgtggaggcagatcagttcatgagggctggcctctacgacggatggctcccgcacctgttcgtggggaacttgctcaagggtcagactgtgggagtcatcggagctggtcgaatcggctcagcttacgcaagaatgatgatcgagggcttcaagatgaacttgatctactacgacctgtatcagtccacgcgccttgagaaattcgtcacagcttacgggcagttcctgaaagcaaacggagagcagcctgtgacatggaagagagccgccaccatggaggatgtcctcagggaggccgacgtgattagtctgcaccctgtgttggacaagaccacttaccatctgataaacccggaaaggctggccatcatgaagaaggaagcggttcttgtgaatgcaagtcgaggaccggtgattgatgaggtagcactggtggagcacctcaaggccaaccccatgttccgcgtcggtcttgatgtgtttgaggatgagccttacatgaagcccggcctcgctgacatgaagaatgccgtcgtcgtacctcacatcgcatctgcctccaagtggacgcgcgaaggaatggcaacacttgctgctctaaacgttcttggcaagatcaagggttatccggtttgggggaacccgaatctagtggagccatttctcaaggaggatgcaacgccgccagctgcttgccccagcattgttaatgccaaacaactcggcctgccatcgtcaaagctctaa</cdnaseq>|
 
AA = <aaseq>MAKPISIEVWNPSGKYRVVSTKSMPGTRWIRLLADNDCRLEICT                    ETKTILSVDDILALIGDRCDGVIGQLTEEWGEVLFSALKRAGGTAFSNMAVGYNNVDV                    EAANRNGIAVGNTPGVLTETTAELAASLSLAAARRIVEADQFMRAGLYDGWLPHLFVG                    NLLKGQTVGVIGAGRIGSAYARMMIEGFKMNLIYYDLYQSTRLEKFVTAYGQFLKANG                    EQPVTWKRAATMEDVLREADVISLHPVLDKTTYHLINPERLAIMKKEAVLVNASRGPV                    IDEVALVEHLKANPMFRVGLDVFEDEPYMKPGLADMKNAVVVPHIASASKWTREGMAT                    LAALNVLGKIKGYPVWGNPNLVEPFLKEDATPPAACPSIVNAKQLGLPSSKL</aaseq>|
 
DNA = <dnaseqindica>2777..2799#2573..2687#2357..2401#1970..2155#1780..1854#1577..1668#1078..1150#877..958#664..785#441..581#289..372#77..199#agtagtagagtgagctcaagacaagaagcagtagcgagtccaaggcaaggcaaggcaggcgaaagcaaaacaagaaatggcgaagccgatatcgatcgaggtgtggaatccgagcggaaagtatcgcgtggtgagcaccaagtccatgcccggcacccgctggatccgcctcctcgccgacaacgactgccgcctcgaggtaatcctttcctttccttcttaggcagcgattacttattgcattacaattacactagtagtggagagtcaattcattccttgcttcagatctgcacggagacgaagaccatcctctctgtcgacgacatcctcgccctcattggcgatcgctgcgatggtgtcatcggccaggcaagcaaagcaattgcaatccaataattttgattgatttcttcttgttgataacaaattaagtgcagctgacggaggagtggggagaggtgctgttctcggcgctgaagcgcgccggcggaacagccttcagcaacatggccgtcgggtacaacaacgtcgacgtggaggccgccaataggaacggcatcgccgtcggcaacacgcctgtcagttctgccgccctctgcttcttgatttctagactatcttactggatggtaatttacttacatacgtacacatgtacagggcgttctgacggagacgacggcggagctggccgcgtctctgtcgctggcggcggcgcggaggatcgtggaggcagatcagttcatgagggctggcctctacgacggatggctcccgcacctgtctctctctctctctctctctctttacttttactactgctgctagttgatagatactcatatatgtatatatgtatatatgtgcatgcaggttcgtggggaacttgctcaagggtcagactgtgggagtcatcggagctggtcgaatcggctcagcttacgcaagaatgatggttagttcagttggtaccttactagctaggactattattattatagtaggagtaattatttgttcaatttcgcaattgcaccacaaaattactcatgtaaacatttccttttaccacagatcgagggcttcaagatgaacttgatctactacgacctgtatcagtccacgcgccttgagaaattcgtcacaggtatgcatccaattttatagtactggctagttaatttgtactccctccgttctaaattgatctacatatttcataggtacaccaagatcaataaaaactaataactctctcatactatatttactctagcaacaaattcaatgcatgcaccatccctaatattttctagccaatagcaaatcaaaatattgcatgtgggttataaatacttgtgtgcatggatgcatgcatcaatgtccatttactccaatgcacaaataacgaatagactctaataaatgaacacaaatgtgtagatcatttagaaataatctaaaaaactatatgtagatcaatttggaatggagggagtacgacattaagatagatagattagattagattaattaatgacctgcacattgatttgaatgcatatgcatgtagcttacgggcagttcctgaaagcaaacggagagcagcctgtgacatggaagagagccgccaccatggaggatgtcctcagggaggccgacgtggtcagccatctctctcatcgtctcttctttctttcttcattctaattattaagatgttgattaattatgcaaataaatgcttgtgcatatatatatatatgtatgtggcagattagtctgcaccctgtgttggacaagaccacttaccatctgataaacccggaaaggctggccatcatgaagaaggtgcctgcctctctgcctctgttaatttcttctctcggtcctcttgatactgtagtactatgcagtgggtaattaacagagcatgtatacaaataatctaaatataaaaatacaggaagcggttcttgtgaatgcaagtcgaggaccggtgattgatgaggtagcactggtggagcacctcaaggccaaccccatgttccgcgtcggtcttgatgtgtttgaggatgagccttacatgaagcccggcctcgctgacatgaagaatgccgtcgtcgtacctcacatcgcatctgcctccaaggtccactacctgcctgctgcatctgcttatatatatatatatgcctagctagcttgttttattttctttctttctttctttcttcccataaaaatatccatccatctttacgtactactactcctacaactactagtaatatgaatcaaaaaggtactgactaatatagtatataatttgcatcattctccaattctccagtggacgcgcgaaggaatggcaacacttgctgctctaaacgttcttgtaagtactccttccatatgcatgcttaattagcctcagcccaaatattatactattgatgatgaatggaacacacactgtgtgagtgggtctgtctgtctgtgcatgtttgtatttgcactagatcgattatataaacctgctgatacggtaataataataataatgcagggcaagatcaagggttatccggtttgggggaacccgaatctagtggagccatttctcaaggaggatgcaacgccgccagctgcttgccccagcattgttaatgccaaacaactcggtcggtactactacgtaccgttagttaattcctattcattgcctagttaaaattaactttaactgcatgcgaatatatatgcgatgcaggcctgccatcgtcaaagctctaagaactggagctcggtccatcatattatatctgtcggttcaagatcatactccactacatgaagtctagatcgaggtgcatatgcttgctgttctagcggagcgggcaacaagctgtcagccagacttttgatatctaatttcacacacacacacacacacacacacacacatatatatatatatatataatatactagctagcttcaattccttgttattataccactagtacgactgtaattttgtgagtcttaaaacatgcgagctacatgtatgttattacacccgtactagctactactgtagattaattactgttacgaaatgaagataccctagctgcctgtttgggaaacgtacgattaatcatcaatgacaaagatttacagggctctagctagctagcactagcagcctgcggagagagagaggaattaattgatattagtaataactagcacatacatatgcatccttgcgtatagtaagtggagtaagtttataagggaagaaataattaagggaaaacctggcttagctgaagaagagcaagcaatactattatattatataatccaataaaagttgaaacaaaaaagagggagcaaattaaagcaaaagctagatatatataatataataatgatggacagagatatatagttagtcgaggaggagtttcttgacgtaattatcgtagacgaagtcggtgaggatgctgtaagctttctcggtggggtggtagctgtcccagaagagatagtcggagacgggggtgcagacggaggaggtgagggcgttgcagaggacggaaacctccaggaggccagtgccgcagcaacccatggtggagtcgctgaagccgtacgcccgtggacgcatcatcatgtccatcaggaagccgtagatgtccatgaaaacaagcttggtcttggtgctcttcttcttggcctgcatctcctccatcctcctcttcatcccggcgttgtacgccaccgcgatctggttgtgcccctccgagcagcgcctctccatgccccccgacatggtcctctgcgacggcacgcacccgatgggcggcatcccgatgatggccaccttcctcgccccggccttcaccagctcgtccacgaacgccgccgcgtggtgcaccagcagcgccgcgtacgaggcgtggtcgtagccgggcctcgcccgcatggtgaagtaggtgttggccacgtcgtcgctgccggcgcagatggcgaagatgcccctggtcatcatgtccgccacccgcgcgtccccggcggcgccgcgcacccgctccttgtactgctggaacagcagcagctggtcgggcatggagatgacggaggccaggcgaggcgtcagcgggtcgaagccggtgccccccgacgcgaagctgacgccggtgaggagctcctcagtcgacagctccggcgacaggtacggcgggagaagctccttcagaccaagacgggaagctgcatatgcattccgatcgatccgttgttagttgttgttgtgcatgcatgtactcctatgagaaatgagaattgacaagagaaagaaaagcatgttgctagttaccgatg</dnaseqindica>|
 
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001052124.1 RefSeq:Os02g0101500]|
 
}}
 
[[Category:Genes]]
 
[[Category:Japonica mRNA]]
 
[[Category:Oryza Sativa Japonica Group]]
 
[[Category:Japonica Genes]]
 
[[Category:Japonica Chromosome 2]]
 
[[Category:Chromosome 2]]
 

Latest revision as of 13:10, 3 October 2016

The rice Os02g0101500 was reported as OsHPR1 in 2014 [1] by researchers from China.

Annotated Information

Figure 4. Subcellular localizations of GFP–HPR1 and GFP-HPR2 fusions in transgenic rice protoplasts.[1].

Gene Symbol

  • Os02g0101500 <=> OsHPR1,HPR1

Function

  • The researchers constructed over-expression of the OsHPR1 gene and RNA interference plants of OsHPR1 and OsHPR2 genes in rice.
  • The phenotypes of all transgenic lines were determined in ambient air and CO 2 -elevated air. The phenotype typical of photorespiration mutants were observed only where activity of both HPR1 and HPR2 were down-regulated in the same line.
  • Double deletion of OsHPR1 and OsHPR2 genes significantly reduces plant growth under normal photorespiration condition
  • These findings demonstrate that two hydroxypyruvate reductases encoded by OsHPR1 and OsHPR2 are involved in photorespiratory metabolism in rice.

Expression

  • Results from RT-PCR, western-blot and enzyme assays showed that HPR1 activity changed significantly in corresponding transgenic lines without any effect on HPR2 activity, which is the same for HPR2.

Subcellular Localization

  • In the present study, the subcellular localization of OsHPR1 protein was first predicted to be located in the peroxisome and cytosol respectively.
  • In agreement with the prediction of HPR1 localization, the spots of HPR1-GFP and PTS1-CFP fusions were quite similar in the co-transformation protoplast, indicating the subcellular localization of HPR1 protein is in the peroxisome.


You can also add sub-section(s) at will.

Labs working on this gene

  • State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, South China Agricultural University, Guangzhou, China,
  • The Chinese University of Hong Kong Shenzhen Institute, Shenzhen, China,
  • State Key Laboratory of Agrobiotechnology, The Chinese University of Hong Kong, Hong Kong, China

References

  1. 1.0 1.1 Ye N, Yang G, Chen Y, Zhang C, Zhang J, Peng X. Two hydroxypyruvate reductases encoded by OsHPR1 and OsHPR2 are involved in photorespiratory metabolism in rice. J Integr Plant Biol. 2014 Feb;56(2):170-80. doi: 10.1111/jipb.12125. Epub 2014 Jan 8. PubMed PMID: 24401104.

Structured Information