Difference between revisions of "Os02g0101500"
(Created page with "{{JaponicaGene|
GeneName = Os02g0101500|
Description = Similar to NADH-dependent hydroxypyruvate reductase (EC 1.1.1.29) (Fragment)|
Version = NM_001052124.1 GI:115443618 G...") |
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| − | + | The rice '''''Os02g0101500''''' was reported as '''''OsHPR1''''' in 2014 <ref name="ref1" /> by researchers from China. | |
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| − | + | ==Annotated Information== | |
| − | + | [[File:Os02g0101500.png|right|thumb|327px|'''Figure 4.''' ''Subcellular localizations of GFP–HPR1 and GFP-HPR2 fusions in transgenic rice protoplasts.<ref name="ref1" />.'']] | |
| − | + | ===Gene Symbol=== | |
| − | + | *'''''Os02g0101500''''' '''<=>''' '''''OsHPR1''''','''''HPR1''''' | |
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| − | + | ===Function=== | |
| − | + | * The researchers constructed over-expression of the '''''OsHPR1''''' gene and RNA interference plants of '''''OsHPR1''''' and '''''OsHPR2''''' genes in rice. | |
| − | + | * The phenotypes of all transgenic lines were determined in ambient air and CO 2 -elevated air. The phenotype typical of photorespiration mutants were observed only where activity of both '''''HPR1''''' and '''''HPR2''''' were down-regulated in the same line. | |
| − | + | * Double deletion of '''''OsHPR1''''' and '''''OsHPR2''''' genes significantly reduces plant growth under normal photorespiration condition | |
| − | + | * These findings demonstrate that two hydroxypyruvate reductases encoded by OsHPR1 and OsHPR2 are involved in photorespiratory metabolism in rice. | |
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| − | + | ===Expression=== | |
| − | + | * Results from RT-PCR, western-blot and enzyme assays showed that '''''HPR1''''' activity changed significantly in corresponding transgenic lines without any effect on '''''HPR2''''' activity, which is the same for '''''HPR2'''''. | |
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| − | + | ===Subcellular Localization=== | |
| − | + | * In the present study, the subcellular localization of '''''OsHPR1''''' protein was first predicted to be located in the peroxisome and cytosol respectively. | |
| − | + | * In agreement with the prediction of HPR1 localization, the spots of HPR1-GFP and PTS1-CFP fusions were quite similar in the co-transformation protoplast, indicating the subcellular localization of HPR1 protein is in the peroxisome. | |
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| − | + | You can also add sub-section(s) at will. | |
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| − | + | ==Labs working on this gene== | |
| − | + | * State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, South China Agricultural University, Guangzhou, China, | |
| − | [[Category:Genes]] | + | * The Chinese University of Hong Kong Shenzhen Institute, Shenzhen, China, |
| − | + | * State Key Laboratory of Agrobiotechnology, The Chinese University of Hong Kong, Hong Kong, China | |
| − | [[Category:Oryza Sativa Japonica Group]] | + | |
| − | [[Category:Japonica | + | ==References== |
| − | + | <references> | |
| − | + | * <ref name="ref1"> | |
| + | Ye N, Yang G, Chen Y, Zhang C, Zhang J, Peng X. Two hydroxypyruvate reductases encoded by OsHPR1 and OsHPR2 are involved in photorespiratory metabolism in rice. J Integr Plant Biol. 2014 Feb;56(2):170-80. doi: 10.1111/jipb.12125. Epub 2014 Jan 8. PubMed PMID: 24401104. | ||
| + | </ref> | ||
| + | </references> | ||
| + | |||
| + | ==Structured Information== | ||
| + | [[Category:Genes]][[Category:Oryza Sativa Japonica Group]][[Category:Japonica Chromosome 2]] | ||
Latest revision as of 13:10, 3 October 2016
The rice Os02g0101500 was reported as OsHPR1 in 2014 [1] by researchers from China.
Contents
Annotated Information
Figure 4. Subcellular localizations of GFP–HPR1 and GFP-HPR2 fusions in transgenic rice protoplasts.[1].
Gene Symbol
- Os02g0101500 <=> OsHPR1,HPR1
Function
- The researchers constructed over-expression of the OsHPR1 gene and RNA interference plants of OsHPR1 and OsHPR2 genes in rice.
- The phenotypes of all transgenic lines were determined in ambient air and CO 2 -elevated air. The phenotype typical of photorespiration mutants were observed only where activity of both HPR1 and HPR2 were down-regulated in the same line.
- Double deletion of OsHPR1 and OsHPR2 genes significantly reduces plant growth under normal photorespiration condition
- These findings demonstrate that two hydroxypyruvate reductases encoded by OsHPR1 and OsHPR2 are involved in photorespiratory metabolism in rice.
Expression
- Results from RT-PCR, western-blot and enzyme assays showed that HPR1 activity changed significantly in corresponding transgenic lines without any effect on HPR2 activity, which is the same for HPR2.
Subcellular Localization
- In the present study, the subcellular localization of OsHPR1 protein was first predicted to be located in the peroxisome and cytosol respectively.
- In agreement with the prediction of HPR1 localization, the spots of HPR1-GFP and PTS1-CFP fusions were quite similar in the co-transformation protoplast, indicating the subcellular localization of HPR1 protein is in the peroxisome.
You can also add sub-section(s) at will.
Labs working on this gene
- State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, South China Agricultural University, Guangzhou, China,
- The Chinese University of Hong Kong Shenzhen Institute, Shenzhen, China,
- State Key Laboratory of Agrobiotechnology, The Chinese University of Hong Kong, Hong Kong, China
