Difference between revisions of "Os02g0101500"
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| − | + | The rice '''''Os02g0101500''''' was reported as '''''OsHPR1''''' in 2014 <ref name="ref1" /> by researchers from China. | |
==Annotated Information== | ==Annotated Information== | ||
| + | [[File:Os02g0101500.png|right|thumb|327px|'''Figure 4.''' ''Subcellular localizations of GFP–HPR1 and GFP-HPR2 fusions in transgenic rice protoplasts.<ref name="ref1" />.'']] | ||
| + | ===Gene Symbol=== | ||
| + | *'''''Os02g0101500''''' '''<=>''' '''''OsHPR1''''','''''HPR1''''' | ||
| + | |||
===Function=== | ===Function=== | ||
| − | + | * The researchers constructed over-expression of the '''''OsHPR1''''' gene and RNA interference plants of '''''OsHPR1''''' and '''''OsHPR2''''' genes in rice. | |
| + | * The phenotypes of all transgenic lines were determined in ambient air and CO 2 -elevated air. The phenotype typical of photorespiration mutants were observed only where activity of both '''''HPR1''''' and '''''HPR2''''' were down-regulated in the same line. | ||
| + | * Double deletion of '''''OsHPR1''''' and '''''OsHPR2''''' genes significantly reduces plant growth under normal photorespiration condition | ||
| + | * These findings demonstrate that two hydroxypyruvate reductases encoded by OsHPR1 and OsHPR2 are involved in photorespiratory metabolism in rice. | ||
===Expression=== | ===Expression=== | ||
| − | + | * Results from RT-PCR, western-blot and enzyme assays showed that '''''HPR1''''' activity changed significantly in corresponding transgenic lines without any effect on '''''HPR2''''' activity, which is the same for '''''HPR2'''''. | |
| + | |||
| + | ===Subcellular Localization=== | ||
| + | * In the present study, the subcellular localization of '''''OsHPR1''''' protein was first predicted to be located in the peroxisome and cytosol respectively. | ||
| + | * In agreement with the prediction of HPR1 localization, the spots of HPR1-GFP and PTS1-CFP fusions were quite similar in the co-transformation protoplast, indicating the subcellular localization of HPR1 protein is in the peroxisome. | ||
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You can also add sub-section(s) at will. | You can also add sub-section(s) at will. | ||
Latest revision as of 13:10, 3 October 2016
The rice Os02g0101500 was reported as OsHPR1 in 2014 [1] by researchers from China.
Contents
Annotated Information
Figure 4. Subcellular localizations of GFP–HPR1 and GFP-HPR2 fusions in transgenic rice protoplasts.[1].
Gene Symbol
- Os02g0101500 <=> OsHPR1,HPR1
Function
- The researchers constructed over-expression of the OsHPR1 gene and RNA interference plants of OsHPR1 and OsHPR2 genes in rice.
- The phenotypes of all transgenic lines were determined in ambient air and CO 2 -elevated air. The phenotype typical of photorespiration mutants were observed only where activity of both HPR1 and HPR2 were down-regulated in the same line.
- Double deletion of OsHPR1 and OsHPR2 genes significantly reduces plant growth under normal photorespiration condition
- These findings demonstrate that two hydroxypyruvate reductases encoded by OsHPR1 and OsHPR2 are involved in photorespiratory metabolism in rice.
Expression
- Results from RT-PCR, western-blot and enzyme assays showed that HPR1 activity changed significantly in corresponding transgenic lines without any effect on HPR2 activity, which is the same for HPR2.
Subcellular Localization
- In the present study, the subcellular localization of OsHPR1 protein was first predicted to be located in the peroxisome and cytosol respectively.
- In agreement with the prediction of HPR1 localization, the spots of HPR1-GFP and PTS1-CFP fusions were quite similar in the co-transformation protoplast, indicating the subcellular localization of HPR1 protein is in the peroxisome.
You can also add sub-section(s) at will.
Labs working on this gene
- State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, South China Agricultural University, Guangzhou, China,
- The Chinese University of Hong Kong Shenzhen Institute, Shenzhen, China,
- State Key Laboratory of Agrobiotechnology, The Chinese University of Hong Kong, Hong Kong, China
