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| − | {{JaponicaGene|
| + | The rice '''''Os04g0201900''''' was reported as '''''OslecRK''''' in 2013 <ref name="ref1" /> by researchers from China. |
| − | GeneName = Os04g0201900|
| + | ==Annotated Information== |
| − | Description = Curculin-like (mannose-binding) lectin domain containing protein|
| + | [[File:Os04g0201900.png|right|thumb|327px|'''Figure 2.''' ''Silencing of OslecRK in rice leads to depression of the germination rate.<ref name="ref1" />.'']] |
| − | Version = NM_001058760.2 GI:297602231 GeneID:4335148|
| + | ===Gene Symbol=== |
| − | Length = 4301 bp|
| + | *'''''Os04g0201900''''' '''<=>''' '''''OslecRK,lecRK''''' |
| − | Definition = Oryza sativa Japonica Group Os04g0201900, complete gene.|
| + | ===Function=== |
| − | Source = Oryza sativa Japonica Group
| + | * The rice '''''OslecRK''''' gene encodes rice lectin receptor-like kinase, which can contribute to both seed germination and plant innate immunity. |
| | + | * '''''OslecRK''''' is a component of the innate immune system in plants |
| | + | * Knocking down the '''''OslecRK''''' gene depresses the expression of a–amylase genes, reducing seed viability and thereby decreasing the rate of seed germination. Moreover, it also inhibits the expression of defense genes, and so reduces the resistance of rice plants to fungal and bacterial pathogens as well as herbivorous insects. |
| | + | * The dual effects of '''''OslecRK''''' may be indicative of progressive adaptive evolution in rice. |
| | + | ===Phenotypic analysis=== |
| | + | * To study the function of OslecRK in plants, RNA interference (RNAi) knockdown experiments were performed in rice. |
| | + | * The seeds of the RNAi lines had low germination rates in 3-day germination tests, ranging from 23.3 to 43.3%. This is appreciably lower than the rate of 83.3% observed for wild-type (WT) seeds (Figure 2a). However, there were no statistically significant phenotypic differences between the RNAi line and the WT during the later vegetative and reproductive periods. |
| | + | * The germination rate of the homozygotic RNAi strain Ri2–10 (Ri) (Figure 2b) was monitored over three successive generations, confirming that its low rate of germination was due to the suppression of OslecRK expression (Figure 2c). |
| | + | * This clearly demonstrates that OslecRK influences the rate of seed germination. |
| | | | |
| − | ORGANISM Oryza sativa Japonica Group
| + | You can also add sub-section(s) at will. |
| − | Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
| + | |
| − | Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
| + | ==Labs working on this gene== |
| − | clade; Ehrhartoideae; Oryzeae; Oryza.
| + | * State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan 430072, China |
| − | |
| + | |
| − | Chromosome = [[:category:Japonica Chromosome 4|Chromosome 4]]|
| + | ==References== |
| − | AP = Chromosome 4:6931873..6936173|
| + | <references> |
| − | CDS = 6931873..6932068,6932662..6935066,6936021..6936173|
| + | * <ref name="ref1"> |
| − | GCID = <gbrowseImage1>
| + | Cheng X, Wu Y, Guo J, Du B, Chen R, Zhu L, He G. A rice lectin receptor-like |
| − | name=NC_008397:6931873..6936173
| + | kinase that is involved in innate immune responses also contributes to seed |
| − | source=RiceChromosome04
| + | germination. Plant J. 2013 Nov;76(4):687-98. doi: 10.1111/tpj.12328. Epub 2013 |
| − | preset=GeneLocation
| + | Oct 15. PubMed PMID: 24033867; PubMed Central PMCID: PMC4285754. |
| − | </gbrowseImage1>| | + | </ref> |
| − | GSID = <gbrowseImage2>
| + | |
| − | name=NC_008397:6931873..6936173
| + | </references> |
| − | source=RiceChromosome04
| + | |
| − | preset=GeneLocation
| + | ==Structured Information== |
| − | </gbrowseImage2>| | + | [[Category:Genes]][[Category:Oryza Sativa Japonica Group]][[Category:Japonica Chromosome 4]] |
| − | CDNA = <cdnaseq>atggcatcgccggcggcgcgcgccgatccgccggaacggagccgccggccgcggcgacggcaaggggagggaggccacgggcgaggggcttcaactcgcagcggcagcaagccggcaactgccaactggtctgcaaagagtactggttcagtgctactgtccccaacttgtgcacaaactcagaagaacatcacattgggctccactttggcaccccagagtcctgcctcttcatggctatcaccctctggcgacttcgcatttggcttccgacctgtagagggcaacacctccttctacctcatcgccgtctggttcaacaagattagcgataagacagttgtctggtatgccaaaaatactgaccaagatccatcgatagtagaagtgccatctgattcttttctccaactcactaatgatggtgcactctcactgaaagaccggtctggtcaggagggctggaatccccaggttaccagtgtggcatatgctagcatgcgcgacaccggcaattttgtgctccttggtgcagacggcacgacaaagtggcagacctttgacatgccgtcagacaccatcttgcctactcaggtgataccttgcaacaagactcgcaacaagtcactccgtgcccgactcgacatcaatgactactccagtggtcggtttctccttgatgtgcaaactgatggaaaccttgccctgtatcttgttgctgttccctctggatccaaataccagcaatattggtccactgacacgactggaaatggctcagagttggtattcagtgagactgggaaggtatactttgcactgacagatggtacacagatcaacatctcatcaggtgcaggaattggatcaatggcagactacttccatcgcgccacactggaccctgacggtgttttccggcaatatgtgtacccgaagaaagcgaatgcaggcatcctaggtggtgagacatggacagcagtgagcatgcagccccagaatatctgccatgccatagtttcagatgttggcagtggcgtctgtgggttcaacagttactgcacctttgatggcaccaggaatcagattgcaagctgccaatgcccaccatggtacaagttcttcgatgagcagaagaagtacaaagggtgtaagcaagactttcaaccgcatagctgcgacttggatgaggcaacggcacttgctcagtttgagctgagaccaatttatggtgtggattggccactgtctgactatgagaaatatgagcccataggacaagatgattgtggaaggctgtgtgtgatcgactgcttctgcgccatggcggtgtacaatcagagcaccagcacttgctggaagaagaagctccctttgtcaaatgggaatatggctgactatgtccagaggaccgttcttctcaaggtgccaagtagcaatagttcgcaatccatgatcagtacaagctccaacaaatggaagaggaacaggaagcactgggtcctagggagttctttgattcttggaacctccatattggtgaactttgcactcatctccattttcctttttggtacttactgtagaatagccacaaaaaagaatatcccattgtcacaagcatcaagtaaatcccaactacccttgaagaccttcacttacaaagagctggagaaggcaacggctggcttccatgagattctaggtgccggtgcctctggtgttgtgtacaagggccagttggaggatgaactcaagaccaacattgcagtgaaaaagattgacaagctccaaccagaaaccgagaaggagttcatggtagaagtcgagaccattgggcagacattccacaagaatttggttagattgcttggtttctgtaatgaaggagccgaaagactgctagtttatgagttcatgaccaatggccctctcaaccgtcttttgttcgacaatagtcgcccacattggaacacccgagttcatatcgcgcttggggtggcaagaggtttgctctacttacatgacgaatgtagcaagcaaatcatccattgtgacataaagccgcagaatatccttcttgatgacaaccttgtggccaagatttcagactttggcctagcaaagcttcttttgacaaaccaaacaaggacaaacaccggtattcggggtactcgaggttatgttgcacctgagtggttcaagaacattgggatctctaccaaggttgatgtttatagcttcggagtgatactactagagcttgtttgctgtaggaggaatgtcgagctagaggttgtcgatgaggaacagacgatagtgacttactgggccaatgactgctaccggtctgggaggattgatttgctggtagagggcgatgatgaggcgatctacaatattaagaaggtggaaagatttgtgacagtggcattgtggtgccttcaggaggacccaagcatgcgacctaacatgctaaaggtgacacaaatgcttgatggtgcagttgcaatccccagtcctcctgatccctgttccttcatcagctcacttccatacgcttctaaatgtggatacgactttttgaagaactacgaagattgcactctaatgactcggcaacaacctgatgatgtgaaaggaaaaggaccaatgtttgttgagacatacatacaacaacagctgaacgaaaagcaagtggcaaactccaccaaaggtgtagccaagttgaatgttgatgcaggtttcaaatag</cdnaseq>|
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| − | AA = <aaseq>MASPAARADPPERSRRPRRRQGEGGHGRGASTRSGSKPATANWS AKSTGSVLLSPTCAQTQKNITLGSTLAPQSPASSWLSPSGDFAFGFRPVEGNTSFYLI AVWFNKISDKTVVWYAKNTDQDPSIVEVPSDSFLQLTNDGALSLKDRSGQEGWNPQVT SVAYASMRDTGNFVLLGADGTTKWQTFDMPSDTILPTQVIPCNKTRNKSLRARLDIND YSSGRFLLDVQTDGNLALYLVAVPSGSKYQQYWSTDTTGNGSELVFSETGKVYFALTD GTQINISSGAGIGSMADYFHRATLDPDGVFRQYVYPKKANAGILGGETWTAVSMQPQN ICHAIVSDVGSGVCGFNSYCTFDGTRNQIASCQCPPWYKFFDEQKKYKGCKQDFQPHS CDLDEATALAQFELRPIYGVDWPLSDYEKYEPIGQDDCGRLCVIDCFCAMAVYNQSTS TCWKKKLPLSNGNMADYVQRTVLLKVPSSNSSQSMISTSSNKWKRNRKHWVLGSSLIL GTSILVNFALISIFLFGTYCRIATKKNIPLSQASSKSQLPLKTFTYKELEKATAGFHE ILGAGASGVVYKGQLEDELKTNIAVKKIDKLQPETEKEFMVEVETIGQTFHKNLVRLL GFCNEGAERLLVYEFMTNGPLNRLLFDNSRPHWNTRVHIALGVARGLLYLHDECSKQI IHCDIKPQNILLDDNLVAKISDFGLAKLLLTNQTRTNTGIRGTRGYVAPEWFKNIGIS TKVDVYSFGVILLELVCCRRNVELEVVDEEQTIVTYWANDCYRSGRIDLLVEGDDEAI YNIKKVERFVTVALWCLQEDPSMRPNMLKVTQMLDGAVAIPSPPDPCSFISSLPYASK CGYDFLKNYEDCTLMTRQQPDDVKGKGPMFVETYIQQQLNEKQVANSTKGVAKLNVDA GFK</aaseq>|
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| − | DNA = <dnaseqindica>4106..4301#1108..3512#1..153#atggcatcgccggcggcgcgcgccgatccgccggaacggagccgccggccgcggcgacggcaaggggagggaggccacgggcgaggggcttcaactcgcagcggcagcaagccggcaactgccaactggtctgcaaagagtactggttcagtggtgattcctgattccctcctgttaatttactcagtgaattggatgccaacagtggagtcgttaggtcgagaatgcatacgatttgtttgatgaattgcttgtagagcccaatagtttgacacttcgattcgttactgttttcagttcagttcagtaaactgtgattcagttcaagttcgttatatgatagaaatatcccaggactagtgagtccaaatacagctacttattgttcatcatgtagaattgaagtgacctagggaacttccgaacttataaaatcggaccaaaatattactctttacactgtatttgacataatctatgcaaaagcatgcctgaaatttatcgcatactaagctgttttagtacaaacatttgttactcagacctgatactgatagtcattatgcaattggcaaaagattcagagacacatgtcacacattttttctgcttcatttttttttagaggagttcaatgtatgcaaacatttcttctgccctgaagtgtagggtagaaaaacattttctcggcaatctatagctagttaacacctgtcaagcctgtctacttcagcaagaatgtcgacgacagcaaatcctccccgtggtgctactaatggccagaattgactagcttgatgccttggtctatttggtctcactagctgttacctgttgtttttgtctggacaagaatttctacggatttactgctatcgtgacacgaattccctattgctgctttctgcgtggcaaattccagtaagtgcttgattagtcaagtatatgtagaattccatggtgacgaagacctttccatgacaagtgttaggatagctctataactacacaagtttggcagctcaccagctgttatcaggggtaggaagcaagagcctgaagcaaagaacaatggttgctctgctactctttcccatgctgttgcagctactgtccccaacttgtgcacaaactcagaagaacatcacattgggctccactttggcaccccagagtcctgcctcttcatggctatcaccctctggcgacttcgcatttggcttccgacctgtagagggcaacacctccttctacctcatcgccgtctggttcaacaagattagcgataagacagttgtctggtatgccaaaaatactgaccaagatccatcgatagtagaagtgccatctgattcttttctccaactcactaatgatggtgcactctcactgaaagaccggtctggtcaggagggctggaatccccaggttaccagtgtggcatatgctagcatgcgcgacaccggcaattttgtgctccttggtgcagacggcacgacaaagtggcagacctttgacatgccgtcagacaccatcttgcctactcaggtgataccttgcaacaagactcgcaacaagtcactccgtgcccgactcgacatcaatgactactccagtggtcggtttctccttgatgtgcaaactgatggaaaccttgccctgtatcttgttgctgttccctctggatccaaataccagcaatattggtccactgacacgactggaaatggctcagagttggtattcagtgagactgggaaggtatactttgcactgacagatggtacacagatcaacatctcatcaggtgcaggaattggatcaatggcagactacttccatcgcgccacactggaccctgacggtgttttccggcaatatgtgtacccgaagaaagcgaatgcaggcatcctaggtggtgagacatggacagcagtgagcatgcagccccagaatatctgccatgccatagtttcagatgttggcagtggcgtctgtgggttcaacagttactgcacctttgatggcaccaggaatcagattgcaagctgccaatgcccaccatggtacaagttcttcgatgagcagaagaagtacaaagggtgtaagcaagactttcaaccgcatagctgcgacttggatgaggcaacggcacttgctcagtttgagctgagaccaatttatggtgtggattggccactgtctgactatgagaaatatgagcccataggacaagatgattgtggaaggctgtgtgtgatcgactgcttctgcgccatggcggtgtacaatcagagcaccagcacttgctggaagaagaagctccctttgtcaaatgggaatatggctgactatgtccagaggaccgttcttctcaaggtgccaagtagcaatagttcgcaatccatgatcagtacaagctccaacaaatggaagaggaacaggaagcactgggtcctagggagttctttgattcttggaacctccatattggtgaactttgcactcatctccattttcctttttggtacttactgtagaatagccacaaaaaagaatatcccattgtcacaagcatcaagtaaatcccaactacccttgaagaccttcacttacaaagagctggagaaggcaacggctggcttccatgagattctaggtgccggtgcctctggtgttgtgtacaagggccagttggaggatgaactcaagaccaacattgcagtgaaaaagattgacaagctccaaccagaaaccgagaaggagttcatggtagaagtcgagaccattgggcagacattccacaagaatttggttagattgcttggtttctgtaatgaaggagccgaaagactgctagtttatgagttcatgaccaatggccctctcaaccgtcttttgttcgacaatagtcgcccacattggaacacccgagttcatatcgcgcttggggtggcaagaggtttgctctacttacatgacgaatgtagcaagcaaatcatccattgtgacataaagccgcagaatatccttcttgatgacaaccttgtggccaagatttcagactttggcctagcaaagcttcttttgacaaaccaaacaaggacaaacaccggtattcggggtactcgaggttatgttgcacctgagtggttcaagaacattgggatctctaccaaggttgatgtttatagcttcggagtgatactactagagcttgtttgctgtaggaggaatgtcgagctagaggttgtcgatgaggaacagacgatagtgacttactgggccaatgactgctaccggtctgggaggattgatttgctggtagagggcgatgatgaggcgatctacaatattaagaaggtggaaagatttgtgacagtggcattgtggtgccttcaggaggacccaagcatgcgacctaacatgctaaaggtgacacaaatgcttgatggtgcagttgcaatccccagtcctcctgatccctgttccttcatcagctcacttccatagtatctcttgcatggtcaaataagttttcatatgcgtcaaaacaataagcatttggtgaatagctttagctatattcagaaggcaaatagttattaatttggcattgtactgtagcaacttttagatctcccatgtgtagctataattgagtgggatacacaactggtccatctctttgagctagatatttatctatatctgttatgtaataaaaaaaggaattatagctatacattcaactaatatttcctagtagaattatagcttctaaaataattcatcgaataatacaatgaatatcttttaatccatattttggaaatgagaagagaaaatagaagatgacaaacgtagtaggagacttcttttttgtgtgtttggaatgtaataaacgtgttactgagacagtagttatagtgtatgagggttgttctggatcgatggcaaaacatatcctaacaatgttttgatagattgcaaagtaaatatgataagttcagtttgaaccgagcggttgccatcggaaaccagttgaacaaaatttttctaaacttttttttactcttttttcaacttgacttgtttgttcaagcgcttctaaatgtggatacgactttttgaagaactacgaagattgcactctaatgactcggcaacaacctgatgatgtgaaaggaaaaggaccaatgtttgttgagacatacatacaacaacagctgaacgaaaagcaagtggcaaactccaccaaaggtgtagccaagttgaatgttgatgcaggtttcaaatag</dnaseqindica>|
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| − | Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001058760.2 RefSeq:Os04g0201900]|
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| − | }}
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| − | [[Category:Genes]] | |
| − | [[Category:Japonica mRNA]]
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| − | [[Category:Oryza Sativa Japonica Group]] | |
| − | [[Category:Japonica Genes]]
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| − | [[Category:Japonica Chromosome 4]] | |
| − | [[Category:Chromosome 4]]
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