Difference between revisions of "Os04g0556000"

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(Created page with "{{JaponicaGene| GeneName = Os04g0556000| Description = Copper-transporting ATPase 1 family protein| Version = NM_001060057.2 GI:297603145 GeneID:4336625| Length = 5640 bp|...")
 
 
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{{JaponicaGene|
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The rice '''''Os04g0556000''''' was reported as '''''OsHMA5''''' in 2013 <ref name="ref1" /> by researchers from Japan.  
GeneName = Os04g0556000|
 
Description = Copper-transporting ATPase 1 family protein|
 
Version = NM_001060057.2 GI:297603145 GeneID:4336625|
 
Length = 5640 bp|
 
Definition = Oryza sativa Japonica Group Os04g0556000, complete gene.|
 
Source = Oryza sativa Japonica Group
 
  
  ORGANISM  Oryza sativa Japonica Group
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==Annotated Information==
            Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
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[[File:Os04g0556000.png|right|thumb|427px|'''Figure 3.''' ''Localization of OsHMA5 in rice roots.<ref name="ref1" />.'']]
            Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
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===Gene Symbol===
            clade; Ehrhartoideae; Oryzeae; Oryza.
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*'''''Os04g0556000''''' '''<=>''' '''''OsHMA5,HMA5'''''
|
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===Function===
Chromosome = [[:category:Japonica Chromosome 4|Chromosome 4]]|
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* '''''OsHMA5''''' is involved in loading Cu to the xylem of the roots and other organs.
AP = Chromosome 4:28228344..28233983|
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===Phenotypic analysis===
CDS = 28228368..28228742,28230666..28232057,28232135..28232389,28232471..28232593,28232868..28233272<br>|
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* Knockout of OsHMA5 resulted in a decreased copper (Cu) concentration in the shoots but an increased Cu concentration in the roots at the vegetative stage.
GCID = <gbrowseImage1>
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* At the reproductive stage, the concentration of Cu in the brown rice was significantly lower in the mutants than in the wild-type rice; however, there was no difference in the concentrations of iron, manganese, and zinc between two independent mutants and the wild type.
name=NC_008397:28228344..28233983
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* The Cu concentration of xylem sap was lower in the mutants than in the wild-type rice.
source=RiceChromosome04
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===Expression===
preset=GeneLocation
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* OsHMA5 was mainly expressed in the roots at the vegetative stage but also in nodes, peduncle, rachis, and husk at the reproductive stage.  
</gbrowseImage1>|
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* The expression was up-regulated by excess Cu but not by the deficiency of Cu and other metals, including zinc, iron, and manganese, at the vegetative stage.
GSID = <gbrowseImage2>
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name=NC_008397:28228344..28233983
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===Subcellular localization===
source=RiceChromosome04
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* Analysis of the transgenic rice carrying the OsHMA5 promoter fused with green fluorescent protein revealed that it was localized at the root pericycle cells and xylem region of diffuse vascular bundles in node I, vascular tissues of peduncle, rachis, and husk.  
preset=GeneLocation
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* Immunostaining with an antibody against OsHMA5 revealed that it was localized to the plasma membrane.
</gbrowseImage2>|
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CDNA = <cdnaseq>atggcggcgagcactcgagccctcttcctctcctgcttccacggaagcggcggcggcggcggcacttccgaggtgagccgccgcctcgtgctgcggccgcggtacccgtccatgccgcggcgaccgaggtcggccgcggtcgccggagagggaggggaaggaggaggaggaggaggcgacggcgatctggaggcggcggccgtgggggcggaggaggaggagaaggtggcggtgttcgaggtgtccgggatgacgtgcgccgcgtgcgcgggctcggtggagaaggccgtcaagcggctccagggcatccacgacgccgccgtcgacgtcctcgggggccgcgcgcaggtcgtcttctacccggcgttcgtctcggaggaaaaaattagggaaaccatccaggatgtgggctttgaagctaagctgattgatgaggaagtcaaggaaaaaaacattctagtatgcaggctgcacataaaaggaatgacctgcacatcttgtgcgagcacagtggaatccatcttgcaagttgttcctggtgttcagagagcttctgtcgcattggctactgaagaggcagaaatccgttatgaccgtagaatcgttactgccagtcaactaacccatgcagttgaagagacgggttttgaagcaatactgatcaccactggagatgatcagagcaggatagacctcaaagtggatggcacactcaacgagagatcaatcatgatagtgaaaagctctgtccaagctcttcctggtgtggaagacataaaagttgatcctgagctccacaagatcaccatctcatacaaacctgaccagacaggtcctcgagacctcattgaagtcattgagtcagctgcctctggtgatcttactgtatcgatatacccagaagcagatggaaggcagcagcatagacatggggagatcaagcgatacaggcagtctttcttgtggagtttagtattcaccatcccggtattcctcacctccatggtgttcatgtacatcccagggctgaaggatgggctcgaaaagaaggttattaacatgatgagcattggtgagctcttgcggtggattttgtcaacgccagttcaatttgtaattggccgcagattttatactggtgcttataaagcgttatcccacggctcatcaaacatggatgtactcattgctcttgggaccaacacagcttacttctactcagtttactccatcctccgagctgcctcctcacataattacatggcaactgatttttttgaaactagttcaatgctcatatcgtttatccttcttggaaagtaccttgagatcttggcaaaagggaagacctctgaggctattgccaagcttatggatcttgcaccagaaactgcaactatgttgatatatgatcatgaagggaatgttgtgggcgagaaggaaattgacagtaggttgattcagaaaaatgatgtgatcaaagtagtcccaggtgggaaagttgcttctgatggctttgttatatggggacagagccatgtgaatgaaagcatgatcaccggagaatcacgacctgtggcaaagaggaagggtgacactgtcataggagggacggtgaatgagaatggtgtgcttcatgtccgtgcaacatttgttggatcagagagtgcccttgcacagattgtacggctagtagagtcagcgcaaatggcaaaagctcctgtacaaaagtttgctgatcagatatctagagtctttgttcccctggtcatcattctttctttgcttacttggcttgcttggttcttagctgggaggcttcatggttaccctaactcatggataccatcttccatggatagctttcaactagctctccaattcgggatatcagttatggttattgcatgcccttgcgccttagggcttgcaactccaacagctgtgatggttgctactggagttggtgcctcacaaggagttcttatcaagggtgggcaagctctggagagtgcacagaaggtcgactgcatcgttttcgacaagacagggacactaaccattgggaagcctgttgtggtcaacacaagactgttaaaaaacatggtcttgcgtgaattctatgcttacgttgcagcagcagaggtcaacagtgagcacccactgggtaaggcagtcgtggagcatgccaagaagttccattcggaagagagccatgtctggactgaagcaagggatttcatttcagtgacagggcacggtgtcaaggcaaaaataagtggcagggctgttatggtgggaaacaagagctttatgttgacttcaggcattgatatcccggtggaagctttggaaatcctaacggaagaagaggagaaggcccaaacagcgatcattgtggctatggatcaagaactcgtgggcattatctctgtatctgatccaataaaaccaaatgcccgcgaagtgatttcgtatctcaaatctatgaaggtggagagtataatggtgactggggacaactgggggactgccaatgccattagctaa</cdnaseq>|
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You can also add sub-section(s) at will.
AA = <aaseq>MAASTRALFLSCFHGSGGGGGTSEVSRRLVLRPRYPSMPRRPRS                    AAVAGEGGEGGGGGGDGDLEAAAVGAEEEEKVAVFEVSGMTCAACAGSVEKAVKRLQG                    IHDAAVDVLGGRAQVVFYPAFVSEEKIRETIQDVGFEAKLIDEEVKEKNILVCRLHIK                    GMTCTSCASTVESILQVVPGVQRASVALATEEAEIRYDRRIVTASQLTHAVEETGFEA                    ILITTGDDQSRIDLKVDGTLNERSIMIVKSSVQALPGVEDIKVDPELHKITISYKPDQ                    TGPRDLIEVIESAASGDLTVSIYPEADGRQQHRHGEIKRYRQSFLWSLVFTIPVFLTS                    MVFMYIPGLKDGLEKKVINMMSIGELLRWILSTPVQFVIGRRFYTGAYKALSHGSSNM                    DVLIALGTNTAYFYSVYSILRAASSHNYMATDFFETSSMLISFILLGKYLEILAKGKT                    SEAIAKLMDLAPETATMLIYDHEGNVVGEKEIDSRLIQKNDVIKVVPGGKVASDGFVI                    WGQSHVNESMITGESRPVAKRKGDTVIGGTVNENGVLHVRATFVGSESALAQIVRLVE                    SAQMAKAPVQKFADQISRVFVPLVIILSLLTWLAWFLAGRLHGYPNSWIPSSMDSFQL                    ALQFGISVMVIACPCALGLATPTAVMVATGVGASQGVLIKGGQALESAQKVDCIVFDK                    TGTLTIGKPVVVNTRLLKNMVLREFYAYVAAAEVNSEHPLGKAVVEHAKKFHSEESHV                    WTEARDFISVTGHGVKAKISGRAVMVGNKSFMLTSGIDIPVEALEILTEEEEKAQTAI                    IVAMDQELVGIISVSDPIKPNAREVISYLKSMKVESIMVTGDNWGTANAIS</aaseq>|
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DNA = <dnaseqindica>25..399#2323..3714#3792..4046#4128..4250#4525..4929#gggggtcacgcgaacacgctgccaatggcggcgagcactcgagccctcttcctctcctgcttccacggaagcggcggcggcggcggcacttccgaggtgagccgccgcctcgtgctgcggccgcggtacccgtccatgccgcggcgaccgaggtcggccgcggtcgccggagagggaggggaaggaggaggaggaggaggcgacggcgatctggaggcggcggccgtgggggcggaggaggaggagaaggtggcggtgttcgaggtgtccgggatgacgtgcgccgcgtgcgcgggctcggtggagaaggccgtcaagcggctccagggcatccacgacgccgccgtcgacgtcctcgggggccgcgcgcaggtcgtcttctacccggcgttcgtctcggtgagtatcgaaggctccgtcgctccgatttttccatatattttttttaaagcatgccgcgtttcttacgggctgcttgcttcccgcacgaccagattgatcagcctgtggatagctaggtaaatttaataaggggaaaaaaatgaatgatgctttgtgtgcgacatttttcggttcatactccataccgttagttgcgggtgcttatacgcaaagtggtttgatcttggaagtaggtacacgggaactttattaaattccaggctcccaggctccgggctcccagctatttagcgcgtagagacaaatacatagctgtgtaacattagcagtagatagacaggtagcatatcagacacactgattaaagcatgtttttatgaacatgttattagaaatgtccatacgcatagtatcacgaaaaaggatttgagtgagctttaattgatgctccacaagttcagaaatacttgaaatgtgaggtgggttcgtgaaaagttctctttgcggtttagaaggttacaagtagacatctttgcttgtgacgttgaactgctagctgcttttggggatcccttgaaacatggattggcctgttggatcccaccaactccttcagctgcaagtattaagatttgcacagacctgtagtttcaatatgaagacgtatttgataaaaaaatggacgtcctgcttattgtttgtagcagacaacctctgcttctggacagaaagtagaaaggaagaacaatctaatgtgcatgtgccatgtaattctaatttacctgatattcccaaaacgactgtttcaagttctttttactgaaatcagaatttcagatttaactaactaattttctgtaccacgtaaactttgtacttacctgttttgcttggcgacttttacactgttgcactcttcattctgttgcaaagttccaaattgttcatttggtgttccttccactagatgcaggcatatgcctgattagttactactaatactacaagttggaatatattttcttattcaggaacaatttgttactcacgtttatcatctcataattacgctctctgatcagtggcgcatttggggatgcaaaattgtaatcttattccccatcattattatcctgaatatattttcggaaaaactaatattttatttgatcaagatgtgtcgcttttaatcagtatcttgaaatttattagaacgaaagggggtgccttttctattattggatgtcattttcccactgtgcaaggctgtaagttcactgttaaagataatttcatttgatccccattgtaggacatgaaacaaaccaaagatatttcaacatagtggttgcttggtcatgcatttccttatttgtgtcaactctctctgcatctagtaacttttggtcagtaatagttagaagatgctggcacacttatggataaagtacattgcaaaggactgatattacaattcgagaaataatctttgatgtaaggattgaagttttgtttacaatcttttgagttgggtggcctgtataacacaaacagtatttcagttatctctgtcaggatcccactatgagctgaagcctgtctttgtcaccgtcccattggatagtgcctctttcttcgtagcgctttatattgattgggaattaaaaagaaagactatttgtgttttggatctgttgaatttctagaaaacatatattagttccattgcattttggataactttgttgttttctgtatttgatcaacatctaacagaacattttcatagtggtaccctttttccattttttcaatagttttacttcatgtttttacattacacatgtccacatgtttcaatccaatttttcttcctttcttatatgtttggaaattgtgctcatcaatgccatttgacaggaggaaaaaattagggaaaccatccaggatgtgggctttgaagctaagctgattgatgaggaagtcaaggaaaaaaacattctagtatgcaggctgcacataaaaggaatgacctgcacatcttgtgcgagcacagtggaatccatcttgcaagttgttcctggtgttcagagagcttctgtcgcattggctactgaagaggcagaaatccgttatgaccgtagaatcgttactgccagtcaactaacccatgcagttgaagagacgggttttgaagcaatactgatcaccactggagatgatcagagcaggatagacctcaaagtggatggcacactcaacgagagatcaatcatgatagtgaaaagctctgtccaagctcttcctggtgtggaagacataaaagttgatcctgagctccacaagatcaccatctcatacaaacctgaccagacaggtcctcgagacctcattgaagtcattgagtcagctgcctctggtgatcttactgtatcgatatacccagaagcagatggaaggcagcagcatagacatggggagatcaagcgatacaggcagtctttcttgtggagtttagtattcaccatcccggtattcctcacctccatggtgttcatgtacatcccagggctgaaggatgggctcgaaaagaaggttattaacatgatgagcattggtgagctcttgcggtggattttgtcaacgccagttcaatttgtaattggccgcagattttatactggtgcttataaagcgttatcccacggctcatcaaacatggatgtactcattgctcttgggaccaacacagcttacttctactcagtttactccatcctccgagctgcctcctcacataattacatggcaactgatttttttgaaactagttcaatgctcatatcgtttatccttcttggaaagtaccttgagatcttggcaaaagggaagacctctgaggctattgccaagcttatggatcttgcaccagaaactgcaactatgttgatatatgatcatgaagggaatgttgtgggcgagaaggaaattgacagtaggttgattcagaaaaatgatgtgatcaaagtagtcccaggtgggaaagttgcttctgatggctttgttatatggggacagagccatgtgaatgaaagcatgatcaccggagaatcacgacctgtggcaaagaggaagggtgacactgtcataggagggacggtgaatgagaatggtgtgcttcatgtccgtgcaacatttgttggatcagagagtgcccttgcacagattgtacggctagtagagtcagcgcaaatggcaaaagctcctgtacaaaagtttgctgatcagatatctagagtctttgttcccctggtaagttcagtttcatcgtggtaattcaacggatatcatttcttatcaattctaaatagtcttcttcatcctttcaggtcatcattctttctttgcttacttggcttgcttggttcttagctgggaggcttcatggttaccctaactcatggataccatcttccatggatagctttcaactagctctccaattcgggatatcagttatggttattgcatgcccttgcgccttagggcttgcaactccaacagctgtgatggttgctactggagttggtgcctcacaaggagttcttatcaagggtgggcaagctctggagagtgcacagaaggtccataaaaaaacattgtacttttagatgcacatgtttgcaaacatcaaattattgacaaattttttatggcatttataggtcgactgcatcgttttcgacaagacagggacactaaccattgggaagcctgttgtggtcaacacaagactgttaaaaaacatggtcttgcgtgaattctatgcttacgttgcagcagcagaggtatgctttcaagcttgctagatctttttgattcaatgcctttattacttgtagcaaaatatcattcctttgtgatatatgaaaaggttggttgagggcatagatgtatgcctagacccacccaagttcaagtcctcatcgaggcgaattcggatgcctatttcttcttaattaattaaaaatgtcacctagttcctcctaggttggttaaggttcttttgttgtgatttgtaaaaagaaacattgctcaaattttatcggttctggtcttcaggtcaacagtgagcacccactgggtaaggcagtcgtggagcatgccaagaagttccattcggaagagagccatgtctggactgaagcaagggatttcatttcagtgacagggcacggtgtcaaggcaaaaataagtggcagggctgttatggtgggaaacaagagctttatgttgacttcaggcattgatatcccggtggaagctttggaaatcctaacggaagaagaggagaaggcccaaacagcgatcattgtggctatggatcaagaactcgtgggcattatctctgtatctgatccaataaaaccaaatgcccgcgaagtgatttcgtatctcaaatctatgaaggtggagagtataatggtgactggggacaactgggggactgccaatgccattagctaagagcttggcattgagaacacagttgcagaagcgaaaccagagcagaaagctgagaaggtgaaagaacttcaggtgagctatcataaaatcttttgcactgtctcctggtcttttttcccgtaccgttatttggttattcacaccctccgatttttttttactcttgcagtcggccggaaggaccgtggcaatggttggtgatggaatcaacgattctccagcgcttgtatcagccgacgttggcctggcgattggcgcaggcactgacgtcgccattgaagcggccgacatcgtcctcatgaagagcaacctggaagacgtgatcaccgccatcgatctgtccagaaaaaccttcttccgcatacggatgaactacgtctgggcgctcggctacaacatcatcggcataccgatcgctgccggggtgctcttcccgtcgacccggttccgcttgccgccatgggtcgccggcgctgcgatggctgcttcctccgttagcgtcgtctgctggtccctgctgttgaggtactacaagagcccaaaactgggccgttgatcatgcagtacacacgcatagcatttgcttagaataagaggtgtagatatctgttgacaatgtgctaatgtggttctaaaagaaatgtgctagtgtgtgttgtgtggcacctgaaatgtgtacattacctacgtatatatgcaccgtgtgtttac</dnaseqindica>|
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==Labs working on this gene==
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001060057.2 RefSeq:Os04g0556000]|
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* Institute of Plant Science and Resources, Okayama University, Kurashiki 710–0046, Japan
}}
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[[Category:Genes]]
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==References==
[[Category:Japonica mRNA]]
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<references>
[[Category:Oryza Sativa Japonica Group]]
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* <ref name="ref1">
[[Category:Japonica Genes]]
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Deng F, Yamaji N, Xia J, Ma JF. A member of the heavy metal P-type ATPase
[[Category:Japonica Chromosome 4]]
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OsHMA5 is involved in xylem loading of copper in rice. Plant Physiol. 2013
[[Category:Chromosome 4]]
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Nov;163(3):1353-62. doi: 10.1104/pp.113.226225. Epub 2013 Sep 24. PubMed PMID:
 +
24064929
 +
</ref>
 +
</references>
 +
 
 +
==Structured Information==
 +
    [[Category:Genes]][[Category:Oryza Sativa Japonica Group]][[Category:Japonica Chromosome 4]]

Latest revision as of 05:16, 9 October 2016

The rice Os04g0556000 was reported as OsHMA5 in 2013 [1] by researchers from Japan.

Annotated Information

Figure 3. Localization of OsHMA5 in rice roots.[1].

Gene Symbol

  • Os04g0556000 <=> OsHMA5,HMA5

Function

  • OsHMA5 is involved in loading Cu to the xylem of the roots and other organs.

Phenotypic analysis

  • Knockout of OsHMA5 resulted in a decreased copper (Cu) concentration in the shoots but an increased Cu concentration in the roots at the vegetative stage.
  • At the reproductive stage, the concentration of Cu in the brown rice was significantly lower in the mutants than in the wild-type rice; however, there was no difference in the concentrations of iron, manganese, and zinc between two independent mutants and the wild type.
  • The Cu concentration of xylem sap was lower in the mutants than in the wild-type rice.

Expression

  • OsHMA5 was mainly expressed in the roots at the vegetative stage but also in nodes, peduncle, rachis, and husk at the reproductive stage.
  • The expression was up-regulated by excess Cu but not by the deficiency of Cu and other metals, including zinc, iron, and manganese, at the vegetative stage.

Subcellular localization

  • Analysis of the transgenic rice carrying the OsHMA5 promoter fused with green fluorescent protein revealed that it was localized at the root pericycle cells and xylem region of diffuse vascular bundles in node I, vascular tissues of peduncle, rachis, and husk.
  • Immunostaining with an antibody against OsHMA5 revealed that it was localized to the plasma membrane.

You can also add sub-section(s) at will.

Labs working on this gene

  • Institute of Plant Science and Resources, Okayama University, Kurashiki 710–0046, Japan

References

  1. 1.0 1.1 Deng F, Yamaji N, Xia J, Ma JF. A member of the heavy metal P-type ATPase OsHMA5 is involved in xylem loading of copper in rice. Plant Physiol. 2013 Nov;163(3):1353-62. doi: 10.1104/pp.113.226225. Epub 2013 Sep 24. PubMed PMID: 24064929

Structured Information