Difference between revisions of "Os03g0693700"
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| − | + | The rice '''''Os03g0693700''''' was reported as '''''Osoxo4''''' in 2013 <ref name="ref1" /> by researchers from India and the USA. | |
==Annotated Information== | ==Annotated Information== | ||
| − | === | + | ===Gene Symbol=== |
| − | + | *'''''Os03g0693700''''' '''<=>''' '''''Osoxo4,oxo4''''' | |
| + | ===Phenotypic analysis=== | ||
| + | * Overexpression of the '''''Osoxo4''''' gene in transgenic plants showed higher enzyme activity of oxalate oxidase (OxO) than nontransgenic control plants, which was visualized by histochemical assays and sodium dodecylsulphate-polyacrylamide gel electrophoresis (SDS-PAGE). | ||
| + | * Transgenic rice leaves were more tolerant than control rice leaves to exogenous OA. Transgenic plants showed a higher level of expression of other defence-related genes in response to pathogen infection. | ||
| + | * More importantly, transgenic plants exhibited significantly enhanced durable resistance to R. solani. The overexpression of Osoxo4 in rice did not show any detrimental phenotypic or agronomic effect. | ||
| + | * The transgenic Osoxo4-overexpressing rice lines and nontrans-genic control plants were subjected to bioassay with a virulent Hyderabad isolate of R. solani AG1-1A. The transgenic lines showed an increased level of sheath blight resistance, as demon- strated by a low sheath blight score in comparison with nontransgenic control plants. | ||
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| − | = | + | [[File:26-Os03g0693700.png|center|thumb|720px|'''Figure 7.''' ''Overexpression of rice oxalate oxidase 4 (Osoxo4) enhances resistance against the sheath blight pathogen Rhizoctonia solani<ref name="ref1" />.'']] |
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You can also add sub-section(s) at will. | You can also add sub-section(s) at will. | ||
==Labs working on this gene== | ==Labs working on this gene== | ||
| − | + | * Plant Molecular Biology and Biotechnology Laboratory, Department of Botany, University of Calcutta, 35, Ballygunge Circular Road, Kolkata 700019, India | |
| − | + | * Center for Diabetes Research, The Methodist Hospital Research Institute, 6670 Bertner, Houston, TX 77030, USA | |
| + | * Division of Crop Science, Indian Council of Agricultural Research (ICAR), Krishi Bhavan, Dr. Rajendra Prasad Road, New Delhi 110001, India | ||
==References== | ==References== | ||
| − | + | <references> | |
| + | * <ref name="ref1"> | ||
| + | Molla KA, Karmakar S, Chanda PK, Ghosh S, Sarkar SN, Datta SK, Datta K. Rice | ||
| + | oxalate oxidase gene driven by green tissue-specific promoter increases tolerance | ||
| + | to sheath blight pathogen (Rhizoctonia solani) in transgenic rice. Mol Plant | ||
| + | Pathol. 2013 Dec;14(9):910-22. doi: 10.1111/mpp.12055. Epub 2013 Jul 1. PubMed | ||
| + | PMID: 23809026. | ||
| + | </ref> | ||
| + | </references> | ||
==Structured Information== | ==Structured Information== | ||
| − | + | [[Category:Genes]][[Category:Oryza Sativa Japonica Group]][[Category:Japonica Chromosome 3]] | |
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Latest revision as of 09:02, 9 October 2016
The rice Os03g0693700 was reported as Osoxo4 in 2013 [1] by researchers from India and the USA.
Contents
Annotated Information
Gene Symbol
- Os03g0693700 <=> Osoxo4,oxo4
Phenotypic analysis
- Overexpression of the Osoxo4 gene in transgenic plants showed higher enzyme activity of oxalate oxidase (OxO) than nontransgenic control plants, which was visualized by histochemical assays and sodium dodecylsulphate-polyacrylamide gel electrophoresis (SDS-PAGE).
- Transgenic rice leaves were more tolerant than control rice leaves to exogenous OA. Transgenic plants showed a higher level of expression of other defence-related genes in response to pathogen infection.
- More importantly, transgenic plants exhibited significantly enhanced durable resistance to R. solani. The overexpression of Osoxo4 in rice did not show any detrimental phenotypic or agronomic effect.
- The transgenic Osoxo4-overexpressing rice lines and nontrans-genic control plants were subjected to bioassay with a virulent Hyderabad isolate of R. solani AG1-1A. The transgenic lines showed an increased level of sheath blight resistance, as demon- strated by a low sheath blight score in comparison with nontransgenic control plants.
Figure 7. Overexpression of rice oxalate oxidase 4 (Osoxo4) enhances resistance against the sheath blight pathogen Rhizoctonia solani[1].
You can also add sub-section(s) at will.
Labs working on this gene
- Plant Molecular Biology and Biotechnology Laboratory, Department of Botany, University of Calcutta, 35, Ballygunge Circular Road, Kolkata 700019, India
- Center for Diabetes Research, The Methodist Hospital Research Institute, 6670 Bertner, Houston, TX 77030, USA
- Division of Crop Science, Indian Council of Agricultural Research (ICAR), Krishi Bhavan, Dr. Rajendra Prasad Road, New Delhi 110001, India
References
- ↑ 1.0 1.1 Molla KA, Karmakar S, Chanda PK, Ghosh S, Sarkar SN, Datta SK, Datta K. Rice oxalate oxidase gene driven by green tissue-specific promoter increases tolerance to sheath blight pathogen (Rhizoctonia solani) in transgenic rice. Mol Plant Pathol. 2013 Dec;14(9):910-22. doi: 10.1111/mpp.12055. Epub 2013 Jul 1. PubMed PMID: 23809026.
