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| − | Please input one-sentence summary here.
| + | The rice '''''Os05g0551000''''' was reported as '''''OsHRZ2''''' in 2013 <ref name="ref1" /> by researchers from Japan. |
| | | | |
| | ==Annotated Information== | | ==Annotated Information== |
| | + | [[File:Os01g0689451.png|right|thumb|427px|'''Figure 4.''' ''Fe-deficiency tolerance of the HRZ-knockdown plants.<ref name="ref1" />.'']] |
| | + | ===Gene Symbol=== |
| | + | *'''''Os05g0551000''''' '''<=>''' '''''OsHRZ2,HRZ2''''' |
| | + | |
| | ===Function=== | | ===Function=== |
| − | Please input function information here.
| + | * '''''OsHRZ2''''' is susceptible to degradation in roots irrespective of iron conditions. |
| | + | * The expression of iron deficiency-inducible genes involved in iron utilization is enhanced in OsHRZ-knockdown plants, mostly under iron-sufficient conditions. |
| | + | * '''''OsHRZ2''''' is iron-binding sensors that may negatively regulate iron acquisition under conditions of iron sufficiency. |
| | + | * '''''OsHRZ2''''' is Fe- and Zn-binding ubiquitin ligases. |
| | + | |
| | + | ===Phenotypic analysis=== |
| | + | * The researchers generated HRZ-knockdown rice lines by RNA interference (RNAi) using partial sequences of '''''OsHRZ1''''' or '''''OsHRZ2''''' (HRZ1i and HRZ2i lines, respectively). |
| | + | * The HRZ2i lines showed reduced '''''OsHRZ2''''' expression in Fe-sufficient and -deficient roots and leaves compared with the non-transformants (NT), and also moderate reduction in '''''OsHRZ1''''' and '''''OsHORZ1''''' expression in Fe-sufficient leaves. |
| | + | * These lines also tended to show reduced expression of '''''OsHRZ1''''' in Fe-deficient roots. HRZ1i line 1 showed only a tendency of slight reduction in the expression of '''''OsHRZ1''''' in roots and '''''OsHORZ1''''' in leaves, but none in OsHRZ2 expression. These knockdown lines grew healthily with no visible phenotype under normal growth conditions. |
| | + | * To analyse the Fe nutrition in these lines, Fe-deficiency treatment was imposed hydroponically. A gradual decrease in leaf chlor-ophyll levels occurred, an indicator of Fe deficiency in plants 2 . The knockdown lines, especially HRZ2i, retained significantly higher levels of leaf chlorophyll than NT under conditions of Fe deficiency. |
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| | ===Expression=== | | ===Expression=== |
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| | ==Labs working on this gene== | | ==Labs working on this gene== |
| − | Please input related labs here.
| + | * Japan Science and Technology Agency, PRESTO, 4-1-8 Honcho, Kawaguchi, Saitama 332-0012, Japan. |
| | + | * Research Institute for Bioresources and Biotechnology, Ishikawa Prefectural University, 1-308 Suematsu, Nonoichi, Ishikawa 921-8836, Japan. |
| | + | * Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan. |
| | | | |
| | ==References== | | ==References== |
| − | Please input cited references here.
| + | <references> |
| − | | + | * <ref name="ref1"> |
| | + | Kobayashi T, Nagasaka S, Senoura T, Itai RN, Nakanishi H, Nishizawa NK. |
| | + | Iron-binding haemerythrin RING ubiquitin ligases regulate plant iron responses |
| | + | and accumulation. Nat Commun. 2013;4:2792. doi: 10.1038/ncomms3792. PubMed PMID: |
| | + | 24253678; |
| | + | </ref> |
| | + | </references> |
| | ==Structured Information== | | ==Structured Information== |
| − | {{JaponicaGene|
| + | [[Category:Genes]][[Category:Oryza Sativa Japonica Group]][[Category:Japonica Chromosome 5]] |
| − | GeneName = Os05g0551000|
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| − | Description = Zinc finger, CHY-type domain containing protein|
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| − | Version = NM_001062780.1 GI:115465290 GeneID:4339530|
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| − | Length = 4628 bp|
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| − | Definition = Oryza sativa Japonica Group Os05g0551000, complete gene.|
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| − | Source = Oryza sativa Japonica Group
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| − | | |
| − | ORGANISM Oryza sativa Japonica Group
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| − | Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
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| − | Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
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| − | clade; Ehrhartoideae; Oryzeae; Oryza.
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| − | |
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| − | Chromosome = [[:category:Japonica Chromosome 5|Chromosome 5]]|
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| − | AP = Chromosome 5:27459120..27463747|
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| − | CDS = 27459287..27459328,27459429..27459597,27459705..27460895,27461552..27461723,27461839..27462060<br>,27462582..27462719,27462828..27463062,27463155..27463214,27463376..27463447<br>,27463525..27463659|
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| − | GCID = <gbrowseImage1>
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| − | name=NC_008398:27459120..27463747
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| − | source=RiceChromosome05
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| − | preset=GeneLocation
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| − | </gbrowseImage1>|
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| − | GSID = <gbrowseImage2>
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| − | name=NC_008398:27459120..27463747
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| − | source=RiceChromosome05
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| − | preset=GeneLocation
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| − | </gbrowseImage2>|
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| − | CDNA = <cdnaseq>atggagaaaatcgagaggcacttcaaaaatgaggaaacaaaggtacttcctcaagctaggattcatttttcatcagagaaacaaagggaacttttatataagagtctctgtgtcataccgttgaagttattggagcgtgttttaccgtggtttgtatcgaagctgaatgatcaggatgcagaagcttttcttcagaatatgtttttggcagcaccttcctctgaagctgcattggtcactcttctctctggttgggcatgcaaaggtcgctcaaagggcacctctaactctggaaaattcatatgcttgacaccaagggcactgagcagcccattggatgaaaatgggtttaaagactgccagttatgtccatgttctttacaatcagatatttgttctaggccagccaagaaatggaatgacacagaatctagtaacataagcaactgctcacaaacggctgatatagcattgacatgtaaaaacagaccttgtcacattcctgggttaagggtagaaattagcaatcttgctgtcaattcgtttgcttctgcagagtccttccgatcgctgtctctcaattattctgctccttcattgtattcaagtcttttttcatgggagacagatgcagccttttctggcccagataacatttcaaggccaattgacacaatattcaaatttcataaggcaattcgcaaagatttggagttcttagatgtggaatctagaaagctcatagatggggatgaatcttcccttcgccaattcatcggaaggtttcgtttactgtggggtctttatagagcacacagcaatgctgaagatgaaattgtatttcctgctcttgaatcgaaagagacactgcacaatgtcagccactcatatactcttgaccacaagcaggaagaagaattatttaaagatatatccactattctttttgagctttcacaattgcatgctgatttgaagcatcctcttggtggtgctgatgcagttggagcgaaccacattcatccatataataggatcgattggtccaaaaagaataatgaacttttgacaaagcttcaaggaatgtgcaagtctatccgggttactctgtctaatcatgtccatagagaagaacttgaattgtggccactatttgataaacatttttctgtagaggagcaggataagattgtaggccgtataattggaagtacaggagctgaggttctgcagtcaatgttaccctgggttacatcagcgcttagtctagatgaacagaacaatatgctggacacatggaggcaagtaactaagaatacaatgtttgatgaatggctaaatgaatggtggaagagatcacctacttcttctggcccttcaagcgatgcctcccatccagaagaagatcatttccaggaaaagttcgatcagagtgaacagatgtttaagcctggttggaaggacatcttccgaatgaaccagagtgagcttgaggctgagatacgaaaggtttctcgtgattctactcttgacccaaggaggaaggcttatctaatccaaaatctcatgaccagccgctggatagctgctcagcagaaatcgccccaacctcagtcagaagatcgcaatggatgtacagtattacctggatgttgtccttcataccgagatccagagaatcagatttttggctgtgagcattacaaaaggaaatgcaaacttgttgctgcatgctgcaataaactgttcacatgcaggttttgtcatgataaagttagtgaccatacaatggaaaggaaagcaacggtggaaatgatgtgcatgcaatgcctgaaagttcaaccagttggtccaaattgccagaccccttcttgcaatgggctatccatggcaaagtattattgcagcgtatgcaagttttttgacgatgaaaggagtgtgtaccattgccccttttgcaatttgtgtcgtcttgggcaaggattgggtattgattttttccattgcatgaagtgcaactgttgcctgggcatgaaattgatagagcataaatgtcgagaaaagatgctagagatgaattgcccaatctgctgcgacttcctatttacatcgagtgcagcagttaaaggtctaccttgtggccatttcatgcattcagcttgctttcaggcgtacacctgtagtcactacacctgcccaatctgctctaaatccttgggagatatgacagtatactttggcatgcttgatggcttgctggccgcagaagagcttcctgaggaataccgggaccggtgccaggatatactttgtaacgattgtgaaagaaaaggaaggtctcggttccattggctgtatcacaaatgcggcttctgcggttcatataacactagagttatcaagattgatagagctgattgttcaacatcagattaa</cdnaseq>|
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| − | AA = <aaseq>MEKIERHFKNEETKVLPQARIHFSSEKQRELLYKSLCVIPLKLL ERVLPWFVSKLNDQDAEAFLQNMFLAAPSSEAALVTLLSGWACKGRSKGTSNSGKFIC LTPRALSSPLDENGFKDCQLCPCSLQSDICSRPAKKWNDTESSNISNCSQTADIALTC KNRPCHIPGLRVEISNLAVNSFASAESFRSLSLNYSAPSLYSSLFSWETDAAFSGPDN ISRPIDTIFKFHKAIRKDLEFLDVESRKLIDGDESSLRQFIGRFRLLWGLYRAHSNAE DEIVFPALESKETLHNVSHSYTLDHKQEEELFKDISTILFELSQLHADLKHPLGGADA VGANHIHPYNRIDWSKKNNELLTKLQGMCKSIRVTLSNHVHREELELWPLFDKHFSVE EQDKIVGRIIGSTGAEVLQSMLPWVTSALSLDEQNNMLDTWRQVTKNTMFDEWLNEWW KRSPTSSGPSSDASHPEEDHFQEKFDQSEQMFKPGWKDIFRMNQSELEAEIRKVSRDS TLDPRRKAYLIQNLMTSRWIAAQQKSPQPQSEDRNGCTVLPGCCPSYRDPENQIFGCE HYKRKCKLVAACCNKLFTCRFCHDKVSDHTMERKATVEMMCMQCLKVQPVGPNCQTPS CNGLSMAKYYCSVCKFFDDERSVYHCPFCNLCRLGQGLGIDFFHCMKCNCCLGMKLIE HKCREKMLEMNCPICCDFLFTSSAAVKGLPCGHFMHSACFQAYTCSHYTCPICSKSLG DMTVYFGMLDGLLAAEELPEEYRDRCQDILCNDCERKGRSRFHWLYHKCGFCGSYNTR VIKIDRADCSTSD</aaseq>|
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| − | DNA = <dnaseqindica>168..209#310..478#586..1776#2433..2604#2720..2941#3463..3600#3709..3943#4036..4095#4257..4328#4406..4540#ttatatttccagcagtcaacgatcaagtgtcctttgagcaggagcacgctgaagaagaacggcggtttaataaatttagatgtttaattgaacaaatccaaataacaggagccagatcaacagcagtggatttttactctgagctatgttcacaagctgatcagataatggagaaaatcgagaggcacttcaaaaatgaggaaacaaaggttagtattttagacataaactctgtagttaaggaggaatgttgtctacttgggtcacgtgaccaatgtagtctaacatgtaattcatctaaatatccaggtacttcctcaagctaggattcatttttcatcagagaaacaaagggaacttttatataagagtctctgtgtcataccgttgaagttattggagcgtgttttaccgtggtttgtatcgaagctgaatgatcaggatgcagaagcttttcttcagaatatgtttttggcaggttattttccaagtttactattttcttctttaaaacaccagttttttttttccaaaaaaaaaaaattctcaacattttactgtttactaatatgttgaactgaacagcaccttcctctgaagctgcattggtcactcttctctctggttgggcatgcaaaggtcgctcaaagggcacctctaactctggaaaattcatatgcttgacaccaagggcactgagcagcccattggatgaaaatgggtttaaagactgccagttatgtccatgttctttacaatcagatatttgttctaggccagccaagaaatggaatgacacagaatctagtaacataagcaactgctcacaaacggctgatatagcattgacatgtaaaaacagaccttgtcacattcctgggttaagggtagaaattagcaatcttgctgtcaattcgtttgcttctgcagagtccttccgatcgctgtctctcaattattctgctccttcattgtattcaagtcttttttcatgggagacagatgcagccttttctggcccagataacatttcaaggccaattgacacaatattcaaatttcataaggcaattcgcaaagatttggagttcttagatgtggaatctagaaagctcatagatggggatgaatcttcccttcgccaattcatcggaaggtttcgtttactgtggggtctttatagagcacacagcaatgctgaagatgaaattgtatttcctgctcttgaatcgaaagagacactgcacaatgtcagccactcatatactcttgaccacaagcaggaagaagaattatttaaagatatatccactattctttttgagctttcacaattgcatgctgatttgaagcatcctcttggtggtgctgatgcagttggagcgaaccacattcatccatataataggatcgattggtccaaaaagaataatgaacttttgacaaagcttcaaggaatgtgcaagtctatccgggttactctgtctaatcatgtccatagagaagaacttgaattgtggccactatttgataaacatttttctgtagaggagcaggataagattgtaggccgtataattggaagtacaggagctgaggttctgcagtcaatgttaccctgggttacatcagcgcttagtctagatgaacagaacaatatgctggacacatggaggcaagtaactaagaatacaatgtttgatgaatggctaaatgaatggtggaagagatcacctacttcttctggcccttcaagcgatgcctcccatccagaaggtctgctcaattaatttccatcccatctcccctcttcaaccacttccaccatattttagtttgctatgttatgtaggcactcactgttttccatttcataaaattgtgtcaaagctatatccttttccccattgaagaaacttctatcgaggcccacttgttatatattgtgttagataaaggtgttatcccttacaatggttatcaacaaataagattggccttctaatgagcaacatataaacttcatacattggttagtttctgtgttatctgaataaaaattatcactaattggacaaatgtagttggccaaaaggaaattcgggaaatgttcaaaagcaaagaagctgtaaaattatatccatgtatttgttgatatgtgataagtgataacaccatgacaacgaggacttcccaattgatgactaatttcaactgtcctagctagcgatagtgcaaatctattatgatgtaaatttgtgataaaagagatttagtgaagttggacttagcataaaggaaggtagaatggtgttagaagagagaaaagtagtgggtctgggatgtatatttatgataaaagattaagcactacagttttcatgatcagattttgaattatcttaactcttgttctttgctttggcctgtagaagatcatttccaggaaaagttcgatcagagtgaacagatgtttaagcctggttggaaggacatcttccgaatgaaccagagtgagcttgaggctgagatacgaaaggtttctcgtgattctactcttgacccaaggaggaaggcttatctaatccaaaatctcatgaccaggtttcttatatgaaattttcagaaaacaattttaggatcttttcctactgttcctccactacaatataggaagttattttgaatgactattgttttgctaattatattttttcagccgctggatagctgctcagcagaaatcgccccaacctcagtcagaagatcgcaatggatgtacagtattacctggatgttgtccttcataccgagatccagagaatcagatttttggctgtgagcattacaaaaggaaatgcaaacttgttgctgcatgctgcaataaactgttcacatgcaggttttgtcatgataaagttagtgaccatacaatggaaaggtaaactcagtgcaaccatgttgtctatttttttgagatttactgccaatgatacttctgtattggtcgcagtagtaatgtgttcaatattcatttttaaacaccaaacaacagacatcaataagcatgcagtggttaaagatttaactacagttagagaaccttgtgataaatttatatctgtgcaaaacagtgaggcagaatgattattttaggcatgacttgtggcctttgtattgtccctttcgttcttctcttcatctgttgttttcctcccaagtaaatgtttggttgaaatgatgcgatggcccaaaaagttgtccaatatgaacttttccgtctttagttttttaatcgctgtatttgcatgataggtgtatacagagttttaagaacatgtgagagaggataggaggcagaaactctcctgttttctatcatattatgtgttggttgttatagctttgttataatataaaatgctaaatgatattgctaagtctggggagctgatatgtcaggaaagcaacggtggaaatgatgtgcatgcaatgcctgaaagttcaaccagttggtccaaattgccagaccccttcttgcaatgggctatccatggcaaagtattattgcagcgtatgcaagttttttgacgatgaaaggtaaaaaaaaaaaaaggacgtggttgctagttttgctaactcgggagattgatggggtccaactttgcaatgttttggtgaccacgctaatagtgaatcttttttcaggagtgtgtaccattgccccttttgcaatttgtgtcgtcttgggcaaggattgggtattgattttttccattgcatgaagtgcaactgttgcctgggcatgaaattgatagagcataaatgtcgagaaaagatgctagagatgaattgcccaatctgctgcgacttcctatttacatcgagtgcagcagttaaaggtctaccttgtggccatttcatgcattcagcttgctttcaggtacaataatctagtcgcattctgatgcatggtttcttgagcgatatctcttactgtgagtagtgctgttctgacaagtttgtaaatttcaggcgtacacctgtagtcactacacctgcccaatctgctctaaatccttgggagatatgacagtaagctacaccacttaaataacattttttttcattcaccatagattatattttctccaactccatatttttaccatacacagaacagcttgtaaaaaattccgttcttctgattcagtagtgcagttaatgtatgttctttaaatcgcgtttctctgcaggtatactttggcatgcttgatggcttgctggccgcagaagagcttcctgaggaataccgggaccggtgccaggtaaattattctcctattcctagttaaaaaaatttcagtggctggggtataccgtttgatatgattgattgttccaggatatactttgtaacgattgtgaaagaaaaggaaggtctcggttccattggctgtatcacaaatgcggcttctgcggttcatataacactagagttatcaagattgatagagctgattgttcaacatcagattaaccaggcgagattttccctctcatattcgaaattccttttgaatatatatattgttgtaaatatacatcaacttaaagaaatccatgcg</dnaseqindica>|
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| − | Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001062780.1 RefSeq:Os05g0551000]|
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| − | }}
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| − | [[Category:Genes]] | |
| − | [[Category:Japonica mRNA]]
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| − | [[Category:Oryza Sativa Japonica Group]] | |
| − | [[Category:Japonica Genes]] | |
| − | [[Category:Japonica Chromosome 5]]
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| − | [[Category:Chromosome 5]]
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