Difference between revisions of "Os12g0612700"
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* '''''OsHox33''''' is a member of the class III homeodomain-leucine zipper gene family which can accelerate leaf senescence in rice. | * '''''OsHox33''''' is a member of the class III homeodomain-leucine zipper gene family which can accelerate leaf senescence in rice. | ||
* '''''OsHox33''''' can regulate the expression of '''''GS1''''' and '''''GS2''''', two senescence-associated genes. | * '''''OsHox33''''' can regulate the expression of '''''GS1''''' and '''''GS2''''', two senescence-associated genes. | ||
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===Phenotypic analysis=== | ===Phenotypic analysis=== | ||
* To reveal the function of OsHox33 in rice, the researchers constructed two RNAi vectors using specific fragments from the 3′-UTR or 5′-end region (5′-UTR and first exon) of '''''OsHox33'''''. These two RNAi constructs and two empty vectors were introduced into wild-type plants to produce transgenic plants. | * To reveal the function of OsHox33 in rice, the researchers constructed two RNAi vectors using specific fragments from the 3′-UTR or 5′-end region (5′-UTR and first exon) of '''''OsHox33'''''. These two RNAi constructs and two empty vectors were introduced into wild-type plants to produce transgenic plants. | ||
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* The researchers randomly selected 15 lines with the 5′-end RNAi construct and 10 lines with the 3′-UTR RNAi construct to obtain T1 generation plants. Four of 15 lines with the 5′-end RNAi construct exhibited leaf senescence phenotypes. These plants initially displayed lesion spots in and around leaf midribs; the spots eventually expanded and increased throughout all parts of the leaves, leading to leaf wilting and death (Figure 2D and E). Two of 10 3′-UTR RNAi construct lines exhibited a similar but more severe phenotype: in addition to the appearance of lesion spots, yellowing occurred at leaf tips and spread to the rest of the leaf, leading to leaf wilting and death (Figure 2A–C). | * The researchers randomly selected 15 lines with the 5′-end RNAi construct and 10 lines with the 3′-UTR RNAi construct to obtain T1 generation plants. Four of 15 lines with the 5′-end RNAi construct exhibited leaf senescence phenotypes. These plants initially displayed lesion spots in and around leaf midribs; the spots eventually expanded and increased throughout all parts of the leaves, leading to leaf wilting and death (Figure 2D and E). Two of 10 3′-UTR RNAi construct lines exhibited a similar but more severe phenotype: in addition to the appearance of lesion spots, yellowing occurred at leaf tips and spread to the rest of the leaf, leading to leaf wilting and death (Figure 2A–C). | ||
* In contrast, two empty transgenic lines exhibited a normal phenotype, indicating that knockdown of OsHox33 accelerated rice leaf senescence. | * In contrast, two empty transgenic lines exhibited a normal phenotype, indicating that knockdown of OsHox33 accelerated rice leaf senescence. | ||
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| + | [[File:Os12g0612700.png|center|thumb|727px|'''Figure 2.''' ''Phenotypes and expression analysis of OsHox33 RNAi lines.<ref name="ref1" />.'']] | ||
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| + | ===Expression=== | ||
| + | *'''''OsHox33''''' is mainly expressed in young organs and actively dividing cells, and may play crucial roles in rice growth and development | ||
You can also add sub-section(s) at will. | You can also add sub-section(s) at will. | ||
Latest revision as of 04:56, 10 October 2016
The rice Os12g0612700 was reported as OsHox33 in 2013 [1] by researchers from China.
Contents
Annotated Information
Function
- OsHox33 plays an important role in age-dependent leaf senescence in rice.
- OsHox33 is a member of the class III homeodomain-leucine zipper gene family which can accelerate leaf senescence in rice.
- OsHox33 can regulate the expression of GS1 and GS2, two senescence-associated genes.
Phenotypic analysis
- To reveal the function of OsHox33 in rice, the researchers constructed two RNAi vectors using specific fragments from the 3′-UTR or 5′-end region (5′-UTR and first exon) of OsHox33. These two RNAi constructs and two empty vectors were introduced into wild-type plants to produce transgenic plants.
- The researchers obtained 47 T0 transgenic plants of 16 independent lines with the RNAi 3′-UTR construct, and 61 transgenic plants of 23 independent lines with the RNAi 5′-end construct.
- The researchers randomly selected 15 lines with the 5′-end RNAi construct and 10 lines with the 3′-UTR RNAi construct to obtain T1 generation plants. Four of 15 lines with the 5′-end RNAi construct exhibited leaf senescence phenotypes. These plants initially displayed lesion spots in and around leaf midribs; the spots eventually expanded and increased throughout all parts of the leaves, leading to leaf wilting and death (Figure 2D and E). Two of 10 3′-UTR RNAi construct lines exhibited a similar but more severe phenotype: in addition to the appearance of lesion spots, yellowing occurred at leaf tips and spread to the rest of the leaf, leading to leaf wilting and death (Figure 2A–C).
- In contrast, two empty transgenic lines exhibited a normal phenotype, indicating that knockdown of OsHox33 accelerated rice leaf senescence.
Figure 2. Phenotypes and expression analysis of OsHox33 RNAi lines.[1].
Expression
- OsHox33 is mainly expressed in young organs and actively dividing cells, and may play crucial roles in rice growth and development
You can also add sub-section(s) at will.
Labs working on this gene
- College of Life Sciences, Tianjin Key Laboratory of Animal and Plant Resistance, Tianjin Normal University, Tianjin 300387, China;
- Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China;
- School of Life Science, Tsinghua University, Beijing 100084, China;
- Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan 430062, China
