Difference between revisions of "Os01g0751600"
(Created page with "{{JaponicaGene|
GeneName = Os01g0751600|
Description = Conserved hypothetical protein|
Version = NM_001050795.1 GI:115439960 GeneID:4324634|
Length = 2951 bp|
Definition ...") |
(→Annotated Information) |
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| − | + | The rice '''''Os01g0751600''''' was reported as '''''This1''''' in 2013 <ref name="ref1" /> by researchers from China. | |
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| − | + | ==Annotated Information== | |
| − | + | [[File:43-Os01g0751600.png|right|thumb|327px|'''Figure 6.''' ''Subcellular localization of THIS-GFP and GFP in onion epidermal cells.<ref name="ref1" />.'']] | |
| − | + | ===Gene Symbol=== | |
| − | + | *'''''Os01g0751600''''' '''''<=>''''' '''''OsThis1''''','''''this1''''' | |
| − | + | ===Function=== | |
| − | + | * '''''OsThis1''''' encodes a class III lipase | |
| − | + | * '''''OsThis1''''' plays fundamental role in regulating branch and spikelet fertility as well as other aspects of developmental control. | |
| − | + | * The relative change in expression of marker genes highlighted the possibility that This1 is involved in phytohormone signalling pathways, such as those for strigolactone and auxin. | |
| − | + | * Thus, '''''OsThis1''''' provides joint control between shoot branching and reproductive development. | |
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| − | + | ===Phenotypic analysis=== | |
| − | + | * Similarly to the high tiller number and dwarf mutants in rice, the increased tiller number of this1 plants is ascribed to the release of tiller bud outgrowth rather than to increased tiller bud formation. | |
| − | + | * In the this1 mutant, however, the accelerated rate of branching was delayed until the stem elongation stage, while other mutants lost the ability to control branching at all developmental stages. The seed-setting rate of this1 was less than half that of the wild type, owing to defects in pollen maturation, anther dehiscence, and flower opening. | |
| − | + | * Histological analyses showed that the mutation in this1 resulted in anisotropic cell expansion and cell division. | |
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| − | + | ===Expression=== | |
| − | + | * To investigate the subcellular localization of THIS1 experimentally, this study constructed a vector in which green fluorescent protein (GFP) was fused in frame to the C terminus of THIS1 under the control of the 35S promoter of the cauliflower mosaic virus. Onion epidermal cells were micro-bombarded with constructs to transiently express either GFP or THIS1-GFP. As shown in Fig. 6, THIS1-GFP localized to the cytoplasm (Fig. 6A–C). | |
| − | < | + | * Plasmolysis of the cells was performed to confirm the cytoplasm localization of the protein, excluding the possibility of targeting to the nucleus and cell wall (Fig. 6D–F). |
| − | + | * In contrast, the fluorescence of GFP alone was observed in both nucleus and cytoplasm (Fig. 6G–I). | |
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| − | + | You can also add sub-section(s) at will. | |
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| − | + | ==Labs working on this gene== | |
| − | [[Category:Genes]] | + | * Key Laboratory of Agro-Ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China |
| − | + | * Biotechnology Research Center, Three Gorges University, Yichang 443002, China | |
| − | [[Category:Oryza Sativa Japonica Group]] | + | * State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China |
| − | [[Category:Japonica | + | ==References== |
| − | + | <references> | |
| − | + | * <ref name="ref1"> | |
| + | Liu W, Zhang D, Tang M, Li D, Zhu Y, Zhu L, Chen C. THIS1 is a putative lipase | ||
| + | that regulates tillering, plant height, and spikelet fertility in rice. J Exp | ||
| + | Bot. 2013 Nov;64(14):4389-402. doi: 10.1093/jxb/ert256. Epub 2013 Oct 1. PubMed | ||
| + | PMID: 24085578. | ||
| + | </ref> | ||
| + | </references> | ||
| + | |||
| + | ==Structured Information== | ||
| + | [[Category:Genes]][[Category:Oryza Sativa Japonica Group]][[Category:Japonica Chromosome 1]] | ||
Latest revision as of 09:23, 10 October 2016
The rice Os01g0751600 was reported as This1 in 2013 [1] by researchers from China.
Contents
Annotated Information
Figure 6. Subcellular localization of THIS-GFP and GFP in onion epidermal cells.[1].
Gene Symbol
- Os01g0751600 <=> OsThis1,this1
Function
- OsThis1 encodes a class III lipase
- OsThis1 plays fundamental role in regulating branch and spikelet fertility as well as other aspects of developmental control.
- The relative change in expression of marker genes highlighted the possibility that This1 is involved in phytohormone signalling pathways, such as those for strigolactone and auxin.
- Thus, OsThis1 provides joint control between shoot branching and reproductive development.
Phenotypic analysis
- Similarly to the high tiller number and dwarf mutants in rice, the increased tiller number of this1 plants is ascribed to the release of tiller bud outgrowth rather than to increased tiller bud formation.
- In the this1 mutant, however, the accelerated rate of branching was delayed until the stem elongation stage, while other mutants lost the ability to control branching at all developmental stages. The seed-setting rate of this1 was less than half that of the wild type, owing to defects in pollen maturation, anther dehiscence, and flower opening.
- Histological analyses showed that the mutation in this1 resulted in anisotropic cell expansion and cell division.
Expression
- To investigate the subcellular localization of THIS1 experimentally, this study constructed a vector in which green fluorescent protein (GFP) was fused in frame to the C terminus of THIS1 under the control of the 35S promoter of the cauliflower mosaic virus. Onion epidermal cells were micro-bombarded with constructs to transiently express either GFP or THIS1-GFP. As shown in Fig. 6, THIS1-GFP localized to the cytoplasm (Fig. 6A–C).
- Plasmolysis of the cells was performed to confirm the cytoplasm localization of the protein, excluding the possibility of targeting to the nucleus and cell wall (Fig. 6D–F).
- In contrast, the fluorescence of GFP alone was observed in both nucleus and cytoplasm (Fig. 6G–I).
You can also add sub-section(s) at will.
Labs working on this gene
- Key Laboratory of Agro-Ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China
- Biotechnology Research Center, Three Gorges University, Yichang 443002, China
- State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China
