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| − | Please input one-sentence summary here.
| + | The rice '''''Os03g0226400''''' was reported as '''''MTP8.1''''' in 2013 <ref name="ref1" /> by researchers from Japan. |
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| | ==Annotated Information== | | ==Annotated Information== |
| | + | [[File:Os03g0226400.png|right|thumb|327px|'''Figure 4.''' ''Subcellular localization of OsMTP8.1.<ref name="ref1" />.'']] |
| | + | ===Gene Symbol=== |
| | + | *'''''Os03g0226400''''' '''<=>''' '''''MTP8.1,OsMTP8.1''''' |
| | ===Function=== | | ===Function=== |
| − | Please input function information here.
| + | * '''''OsMTP8.1''''' encodes a cation diffusion facilitator (CDF) family member. |
| | + | * The expression of '''''OsMTP8.1''''' was found to enhance Mn accumulation and tolerance in S. cerevisiae. |
| | + | * Disruption of OsMTP8.1 resulted in decreased chlorophyll levels, growth inhibition in the presence of high concentrations of Mn, and decreased accumulation of Mn in shoots and roots. However, there was no difference in the accumulation of other metals, including Zn, Cu, Fe, Mg, Ca, and K. |
| | + | * '''''OsMTP8.1''''' is an Mn-specific transporter that sequesters Mn into vacuoles in rice and is required for Mn tolerance in shoots. |
| | | | |
| | ===Expression=== | | ===Expression=== |
| − | Please input expression information here.
| + | * In plants, OsMTP8.1 and its transcript were mainly detected in shoots. High or low supply of Mn moderately induced an increase or decrease in the accumulation of OsMTP8.1, respectively. |
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| − | ===Evolution=== | + | ===Subcellular localization=== |
| − | Please input evolution information here.
| + | * OsMTP8.1:GFP fusion protein was transiently expressed in onion epidermal cells. The fluorescence of cells transfected with GFP alone was detected in the nucleus and cytoplasm (Fig. 4A). In contrast, the fluorescence of the fusion protein was observed at the cell periphery (Fig. 4B) but not in the nucleus (magnified view in Fig. 4B), suggesting that OsMTP8.1 localized to the tonoplast. |
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| | You can also add sub-section(s) at will. | | You can also add sub-section(s) at will. |
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| | ==Labs working on this gene== | | ==Labs working on this gene== |
| − | Please input related labs here.
| + | * Faculty of Agriculture, Kochi University, Nankoku 783-8502 Japan |
| | + | * Institute of Plant Science and Resources, Okayama University, Kurashiki 710-0046, Japan |
| | + | * Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657, Japan |
| | + | * Laboratory of Cell Dynamics, Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya 464-8601, Japan |
| | | | |
| | ==References== | | ==References== |
| − | Please input cited references here.
| + | <references> |
| | + | * <ref name="ref1"> |
| | + | Chen Z, Fujii Y, Yamaji N, Masuda S, Takemoto Y, Kamiya T, Yusuyin Y, Iwasaki |
| | + | K, Kato S, Maeshima M, Ma JF, Ueno D. Mn tolerance in rice is mediated by MTP8.1, |
| | + | a member of the cation diffusion facilitator family. J Exp Bot. 2013 |
| | + | Nov;64(14):4375-87. doi: 10.1093/jxb/ert243. Epub 2013 Aug 20. PubMed PMID: |
| | + | 23963678; PubMed Central PMCID: PMC3808320. |
| | + | </ref> |
| | + | </references> |
| | | | |
| | ==Structured Information== | | ==Structured Information== |
| − | {{JaponicaGene|
| + | [[Category:Genes]][[Category:Oryza Sativa Japonica Group]][[Category:Japonica Chromosome 3]] |
| − | GeneName = Os03g0226400|
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| − | Description = Similar to Cation diffusion facilitator 8|
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| − | Version = NM_001055974.1 GI:115451676 GeneID:4332125|
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| − | Length = 4876 bp|
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| − | Definition = Oryza sativa Japonica Group Os03g0226400, complete gene.|
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| − | Source = Oryza sativa Japonica Group
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| − | | |
| − | ORGANISM Oryza sativa Japonica Group
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| − | Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
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| − | Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
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| − | clade; Ehrhartoideae; Oryzeae; Oryza.
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| − | |
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| − | Chromosome = [[:category:Japonica Chromosome 3|Chromosome 3]]|
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| − | AP = Chromosome 3:6664011..6668886|
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| − | CDS = 6664012..6664321,6666308..6666480,6666562..6666760,6666941..6667119,6667212..6667362<br>,6667701..6667834,6667930..6668109|
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| − | GCID = <gbrowseImage1>
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| − | name=NC_008396:6664011..6668886
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| − | source=RiceChromosome03
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| − | preset=GeneLocation
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| − | </gbrowseImage1>|
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| − | GSID = <gbrowseImage2>
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| − | name=NC_008396:6664011..6668886
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| − | source=RiceChromosome03
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| − | preset=GeneLocation
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| − | </gbrowseImage2>|
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| − | CDNA = <cdnaseq>gagacagggagagaagagagagagagagagagagagagcggaggagagaggcggctcgccggctcgagctcgactgctccccactcgcggccgggagggagggagggggagagaaaggagagaggtgattcgatggaggcgaagggggagaatgacgcgcgggcgccgctcctggcggagcggcggcgcaactcggtcgggtcgatgcgcggggagttcgtgtcgcggctgcccaagaaggtgctcgacgccgtcgaccccgagaggccgtcccacgtcgacttctcccgctccaagggcctccgcgaaggagagaaagaatactatgagaaacaatttgccaccttgagatcctttgaggaagttgattcaatagaagaatccaatgtaatgagtgaagaagatgatattgcggagcaaaagcagagtgagtttgccatgaagatatcaaactatgcaaacatgattcttttggcgttgaagatatatgctacaattaaaagtggatcaattgctatagctgcctcgacattggactcgctgcttgatctcatggctggtggtatcctttggttcacacatctctcgatgaagagcatcaacgtctacaagtatcctattggtaaactgagggtgcagcctgttggaatcatcatctttgctgctgtaatggctacattaggattccaagtatttgttcaagctgtggaaaagctgatagtgaacgagactccagataaattgactccagtgcagctcacatggctatattctatcatgatttttgcaacagtagttaagctagccctctggctctactgcagaacatctggtaacaagatagtccgtgcctatgctaaggatcattattttgacgtcgtcacgaatgttgtgggtttggctgctgctgtccttggtgatatgttctactggtggattgacccagttggtgcaattgcccttgcagtgtacacaattacaaactggtctggaacggtgtgggaaaatgcagtgtcactagtgggtgaatcagctccgccagaaatgctgcagaagttgacatatttggccatcagacatcatcctcaaatcaagcgtgtcgatacagttcgagcatacacctttggggttctttactttgttgaggttgacattgagctgccagaggaattgccccttaaggaggcacatgctattggagaatcacttcaaataaagatcgaggagctcccagaagtcgagcgggcatttgttcaccttgatttcgagtgtgaccacaagccagagcacaacattctcagcaagttgcctagcagccaaccatga</cdnaseq>|
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| − | AA = <aaseq>ETGREERERERESGGERRLAGSSSTAPHSRPGGREGERKERGDS MEAKGENDARAPLLAERRRNSVGSMRGEFVSRLPKKVLDAVDPERPSHVDFSRSKGLR EGEKEYYEKQFATLRSFEEVDSIEESNVMSEEDDIAEQKQSEFAMKISNYANMILLAL KIYATIKSGSIAIAASTLDSLLDLMAGGILWFTHLSMKSINVYKYPIGKLRVQPVGII IFAAVMATLGFQVFVQAVEKLIVNETPDKLTPVQLTWLYSIMIFATVVKLALWLYCRT SGNKIVRAYAKDHYFDVVTNVVGLAAAVLGDMFYWWIDPVGAIALAVYTITNWSGTVW ENAVSLVGESAPPEMLQKLTYLAIRHHPQIKRVDTVRAYTFGVLYFVEVDIELPEELP LKEAHAIGESLQIKIEELPEVERAFVHLDFECDHKPEHNILSKLPSSQP</aaseq>|
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| − | DNA = <dnaseqindica>2..311#2298..2470#2552..2750#2931..3109#3202..3352#3691..3824#3920..4099#ggagacagggagagaagagagagagagagagagagagagcggaggagagaggcggctcgccggctcgagctcgactgctccccactcgcggccgggagggagggagggggagagaaaggagagaggtgattcgatggaggcgaagggggagaatgacgcgcgggcgccgctcctggcggagcggcggcgcaactcggtcgggtcgatgcgcggggagttcgtgtcgcggctgcccaagaaggtgctcgacgccgtcgaccccgagaggccgtcccacgtcgacttctcccgctccaagggcctccgcgaaggtaccacgctggacagccctccccgcattacttctttgtaaagcgcgttctgatctttcccccgttttcgatctgtcctttgtccttttaggtttggctgtggcaacgttgcatgaaagttgtgattctttttaggctctcctttttgttagtcatcacttgggtgttggtgagtttgtctttggggtttggctgtagtagcttttcagaattacggtggataatgccgttcttgctggtgatcgcgcggtcgatgttgttgtatggatggaaatcgccttctattcttgattcaccatccacttgcgcgtgcttggtagttgtgtgtcgtcctttggtataccgtttgacggtgacggatcaagttcgtgggtttactatcttttggttcgcatttttacggtcttttcatatggttttagcgattgtaacatattgaatgactcctaatagaatgacacgcagatctccttctggttctaaagaataacaggtggcgatttatcccgactataatgttttcatgcatgctcctaggcaaaggtcattcgttatatgttctttaaaaaatgctaagcagctatcaattgtgtacgaaaaatctttcaggacaatgttatttctttcttgctctgatgctctctctaacggacatgctcttttttttttagccaaagggggaagaagagtacatagttcaagagatacaaccaatataaagaaaaggcggtagcttgggtagggggagggctatctcgcagcccatctagagttcttttttttaaaaaaaaaatctaaattgctagaaaggaaactgttgtcgaagttaaaggaattacacatcaaggagaacatctccgtttgatctttctgtctcctctgcatttcttctttgtccaggagaaccaggacttccactgttccacagtctatttcctccaccagaacagtactttttctccggtcctggtcaacgtaatacaaaacatgggtataccggcctttgtttacctgaaattttagctgtgccttttttaattctgcctgcctgccgttcaattcattttagcactatatacttccctttccaactttggtctttatgtcttttgaaattgtagcttagatactaaaacacgtcaagactaaattacagtttgtcaaaccgaggtgatatgtagattatacaaaaaccagccggcgttggctgttaataccaatatatttgtaaggatgttctgcatctaccagtggaaatttttgacttctctaccaatagcatgtactaaaaatttggttacgagatttcattggttgtacatttaattattgtattgccattttgttccctgtctctttctttaaaataagtgaggtgggtgggatgtattttatttatttttaatccacgatttgatgtaaccctgcctagctgtatcaggattgtgagactgtcaattgtcgctaagtggaaaaacatacacctttacttttcataaacgactacaaactgtgttaaaaatggtgggatgaaaacacttaatgcaaatattacgaaataagtaatactctttgtatcagggagagaaatatagaagtataatgtggtgtttactctttagtatttcttatgcagtaagttctcagttgtatgaatcaccacatatctttcttgtactgacattaagtatagagataagatttgcaaatcctggactataataaactagtttttattgttcctatattcacatcattttttagagtttgttcttattaataattttttcactacaaactcttaatataactgttaagataaggtgtgcccaatgtgttatacggcttattcctaatatttcataaccctggacaaactccccatgatccatatgaagggtaacaccaattaaattctctctttttatatgctcaaaagacactgctttccttcatgtgatatggttaccttttgggctaatataggagagaaagaatactatgagaaacaatttgccaccttgagatcctttgaggaagttgattcaatagaagaatccaatgtaatgagtgaagaagatgatattgcggagcaaaagcagagtgagtttgccatgaagatatcaaactatgcaaacatgattcttttggcgttgaaggttgtactttcccgattcattttaatcccttttttaacattcatctaggctgactttattatacttccacaccatcattagatatatgctacaattaaaagtggatcaattgctatagctgcctcgacattggactcgctgcttgatctcatggctggtggtatcctttggttcacacatctctcgatgaagagcatcaacgtctacaagtatcctattggtaaactgagggtgcagcctgttggaatcatcatctttgctgctgtaatggctacattaggtatgcttgcaactttgccaggacaagaatggacaatcaattcatgtctttacttgctttctttgtatatgcaacagtattgttgtcttcaaactacaaaatttattactcaacttagtatggtctttgtgccaaaattatgcattaacacagcaatgtgctttttatttgcatttccaggattccaagtatttgttcaagctgtggaaaagctgatagtgaacgagactccagataaattgactccagtgcagctcacatggctatattctatcatgatttttgcaacagtagttaagctagccctctggctctactgcagaacatctggtaacaagatagtccgtgcctatgctaaggtttagatctttctccatgttttaatttcaatagatgctttgaaaggcaaatgagtgcagtttcccaaagtttgttgggttggtattcacaggatcattattttgacgtcgtcacgaatgttgtgggtttggctgctgctgtccttggtgatatgttctactggtggattgacccagttggtgcaattgcccttgcagtgtacacaattacaaactggtctggaacggtgtgggaaaatgcaggtttgcagtactccagcatctgttgcattcgtttataattggcctacccccctcccacatccattatctgttgtcgtagtaaaaagtttcataccagaactcttgttaggatcaattgctttgataggcctaatggacatggaaatctgatgaactaaacccatgcaagaagcaggattgtagggaacatgtttttccgaaaaaaaatatataaattgactcaccatataagtgtaattcctgcacaattccttatgtgttctgaaagttacttactagattaactggcagatagtcgtatactgaattaatgatgcttattgctatccttgatgcagtgtcactagtgggtgaatcagctccgccagaaatgctgcagaagttgacatatttggccatcagacatcatcctcaaatcaagcgtgtcgatacagttcgagcatacacctttggggttctttactttgttgaggttggtacctcagtttttctgttgccttgctgtttttttcccttttaggtaaatatatatgctcttcccagctaatttgatcaacggactggcaggttgacattgagctgccagaggaattgccccttaaggaggcacatgctattggagaatcacttcaaataaagatcgaggagctcccagaagtcgagcgggcatttgttcaccttgatttcgagtgtgaccacaagccagagcacaacattctcagcaagttgcctagcagccaaccatgaccaccaacttgaattccaccgtgaaacgaattagctggcttgttcgcttgccatgcaggatgtgctacagtacgtgccgcattatgctatatgaaacggctgaagacaaatgagattgtctggccatcatttctgaaagtaggcctaagagaatactgtattagttaagtacctatattagagattatgtggactgaacttgtgcatccctctgtttaagagttttgttgtgaggaaagttcagcacaaaggggaaatcacgttctggatgatctcaagcaatttttttgttctgaaattcaggtcagttgtaaatattgtctggtttgcttaacaatggtgtgtaagtgccctacattgctgggaaggtcaaggtgtatgtgctaaaagaaactcattttaacaaatgtggttttgcctttttcagcccaacatcaggcccagcactctttccaaattgagaaaggaaaacagaactggcccagtaggtgtaaactggatgcaaagcaatgcacccggcaaacatccagcaaacaacaagcagtaaaaatttacaacgtaaagcctcttcgatagaccggatattttcatttattgaatttgaaattcttttgaaatttcttcacagtgcaagtacttctattaggattgtaggatgattcttctgcagttgtaccctttgtcactagtggttgtaccattgcagtccgtagtacggccgtgggactgtctgtaagccacgcattattggctgaaatgctgaattttagtagatgg</dnaseqindica>|
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| − | Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001055974.1 RefSeq:Os03g0226400]|
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| − | }}
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| − | [[Category:Genes]] | |
| − | [[Category:Japonica mRNA]]
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| − | [[Category:Oryza Sativa Japonica Group]] | |
| − | [[Category:Japonica Genes]] | |
| − | [[Category:Japonica Chromosome 3]]
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| − | [[Category:Chromosome 3]]
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