Difference between revisions of "Os01g0527600"

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(Created page with "{{JaponicaGene| GeneName = Os01g0527600| Description = RNA dependent RNA polymerase family protein| Version = NM_001049764.1 GI:115437171 GeneID:4323938| Length = 2645 bp|...")
 
(→‎Function)
 
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{{JaponicaGene|
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The rice '''''Os01g0527600''''' was reported as '''''OsRDR6''''' in 2012 <ref name="ref1" /> by researchers from China.  
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GeneName = Os01g0527600|
 
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Description = RNA dependent RNA polymerase family protein|
 
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Version = NM_001049764.1 GI:115437171 GeneID:4323938|
 
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Length = 2645 bp|
 
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Definition = Oryza sativa Japonica Group Os01g0527600, complete gene.|
 
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Source = Oryza sativa Japonica Group
 
  
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  ORGANISM  Oryza sativa Japonica Group
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==Annotated Information==
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            Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
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[[File:51-Os01g0527600.png|right|thumb|427px|'''Figure 1.''' ''Characterization of the rice temperature-sensitive mutant osrdr6-1.<ref name="ref1" />.'']]
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            Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
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===Gene Symbol===
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            clade; Ehrhartoideae; Oryzeae; Oryza.
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*'''''Os11g0267000''''' '''<=>''' '''''OsRDR6,OsSHL2,RDR6,SHL2'''''
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|
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Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|
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===Function===
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AP = Chromosome 1:20597234..20599878|
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* The rice '''''OsRDR6''''' acts in the biogenesis of various types and sizes of small RNAs.
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CDS = 20597487..20598356,20599590..20599877|
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* OsRDR6-dependent tasiR-ARFs play key roles in lemma development in rice.
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GCID = <gbrowseImage1>
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* '''''OsRDR6''''' is required for the biogenesis of 24-nt phased small RNAs.
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name=NC_008394:20597234..20599878
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* '''''OsRDR6'''''-dependent 24-nt phased small RNAs are independent of the RNA-directed DNA methylation (RdDM) pathway.
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source=RiceChromosome01
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* '''''OsRDR6''''' mediates secondary small RNA biogenesis in rice.
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preset=GeneLocation
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</gbrowseImage1>|
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===Phenotypic analysis===
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GSID = <gbrowseImage2>
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* The researchers identified a spontaneous rice mutant with pleiotropic spikelet defects, with most spikelets showing needle-shaped, awn-like lemma; this phenotype is similar to that of shl2-rol and osdcl4-1 mutants (Liu et al., 2007; Nogueira et al., 2007; Toriba et al., 2010).
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name=NC_008394:20597234..20599878
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* Unlike the con- sistent spikelet defects in shl2-rol and osdcl4-1, the osrdr6-1 mutants showed various degrees of spikelet defects under different natural growth conditions.  
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source=RiceChromosome01
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* The spikelet defects and altered stamen number of the mutant only occurred at high temperatures and under long-day conditions, as found in July in Hangzhou. Mutant plants were normal when grown in low temperatures and short-day conditions, as found in October at the same location .
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preset=GeneLocation
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* The developmental defects of osrdr6-1 were rescued by a construct containing the full-length '''''OsRDR6''''' genomic DNA; the transgenic plants ap- peared morphologically identical to wild-type Zhonghua11 (Figure 1b), confirming that the OsRDR6 locus is responsible for the mutant phenotype.
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</gbrowseImage2>|
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CDNA = <cdnaseq>attttgggtactgtggtaatagcaaagaatccctgtcttcatccaggggatgtccgcatccttgaagcagttgatgtgcccgaactgcatcatctggttgattgtttggtgttcccccagaaaggtgagaggccacatgctaacgaggcatctgggagcgatcttgatggggatctctactttgtgacatgggatgagaaacttatacctccaggcaagaagagctggaaccctatggactactccccacctgaagcaaaacaacttccgcgccaagtatctcaacatgatatcattgatttcttcttaaagaacatgataagtgagaatcttggtaggatctgtaatgctcatgttgttcatgctgatcttagcgagtatggtgcaatggatgagaagtgtattcacttagctgagctagcagcaactgccgtggacttccccaagactggcaaacttgcgataatgccaccacaccttaaaccaaaagtctaccctgacttcatgggaaaggaagatggacaatcttataaatcagaaaagattcttggaaggctttatcgttcaatccaagaggcctccaatggtgatgtggtgtcacaagaggtttgcactccaaatgatctgccttatgacatagatctggaagttcctggtgcatcagatttcctcgcgagtgcttggcaatgcaagtgttcatacgacgcgcagctgagtgcgctgctcagtcagtacagggtccgcactgaagctgaacttgtgacagggcacataacgttccttgttaagaacagcagcaagaagcaaggcgacataaaggacaggctgaagactgcttactctgcactacgcaaggagttcaaaagtacctttgagagcatagcatcggatcaatgcgagattggtgatgacgagaagaatctgctgtacgagatgaaggcctctgcctggtaccaggtgacctatcaccccaaatgggtggagaagtcgaggggcattctgggcccagatggtgaggaaataccggcaagccttagctttgcatggatcccggtggattacttggcgcggataaagctaaggtgccatggtaaagtgagagttgaaggccaaaagcctgttgaaaggcttgcagcatacatctccgagaggatatga</cdnaseq>|
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AA = <aaseq>ILGTVVIAKNPCLHPGDVRILEAVDVPELHHLVDCLVFPQKGER                    PHANEASGSDLDGDLYFVTWDEKLIPPGKKSWNPMDYSPPEAKQLPRQVSQHDIIDFF                    LKNMISENLGRICNAHVVHADLSEYGAMDEKCIHLAELAATAVDFPKTGKLAIMPPHL                    KPKVYPDFMGKEDGQSYKSEKILGRLYRSIQEASNGDVVSQEVCTPNDLPYDIDLEVP                    GASDFLASAWQCKCSYDAQLSALLSQYRVRTEAELVTGHITFLVKNSSKKQGDIKDRL                    KTAYSALRKEFKSTFESIASDQCEIGDDEKNLLYEMKASAWYQVTYHPKWVEKSRGIL                    GPDGEEIPASLSFAWIPVDYLARIKLRCHGKVRVEGQKPVERLAAYISERI</aaseq>|
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You can also add sub-section(s) at will.
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DNA = <dnaseqindica>1523..2392#2..289#cattttgggtactgtggtaatagcaaagaatccctgtcttcatccaggggatgtccgcatccttgaagcagttgatgtgcccgaactgcatcatctggttgattgtttggtgttcccccagaaaggtgagaggccacatgctaacgaggcatctgggagcgatcttgatggggatctctactttgtgacatgggatgagaaacttatacctccaggcaagaagagctggaaccctatggactactccccacctgaagcaaaacaacttccgcgccaagtatctcaacatgtaagttcaataactcttgagatgtcagcaatgtcccataattttttgtttcctacatacatgtcctttttatacgatggataaccctcctttcattggaagcatatatgggttttctttacagaactgagataaagaaattaaacgcaagctatggtcgggcacactggcacaccagcaaaaacagggaatagctttagaatctcagatatattacagcaatcaaaattaagtccaaaccagagaggtcctcctagcatttgtaaaccttataggcatcatctccctgctgaactaccgaggccatttcttcacatttgtgacttccctatcttctttacgtagcattaccttccgtttctgcaattacaactttatataaagtttaaatcaaccagctgttgaaggagtacatgattaattgccatctgtgtttctacctgtccaaatcacccctgaaaaaacatcaaacataacattttcaaccatttagggcctcacacctggatccaaggaaatccttgattggagttgggtgctctgtcctatctaagagcttttgatgcatatccatatgaaccctgataatttgcatgtaactagcaggttatcgcttgctcaaacgctgggcaactagtctctagtcttggtcgagtgtgtggttgacttggtcgtggacatgcttggttcttagatcgtgttttgtattcagggtttcttataccctttctttcttgcactgtccatattctaatgcaaatttagaagtaatgattttcatcttccttgttattttattatttggttatactatttgtggtttctagctgtcaacaagtaatggttgtggtttccttcatagttttagtatggttttgtgatatttacgaagttaagctttagcaaactattagcatagcatggttggttagcgctcattttattcaccatctgaatttacctcatgttatcatatattcctacttggcaagctgccaacttcaaattggaatatggtgatgtatttacaaagttgttaccatgtagattgttatttggatttgaaatctggcattggtgatacattttctccaatcgcatatgcatatagctgcttaaaaaactgactcatattaattcccctagtttctgcaaattgtagatttcttgatttgtgggcaagtatctattgctataatgcatatcattgactagatcttgtttttgtaacaggatatcattgatttcttcttaaagaacatgataagtgagaatcttggtaggatctgtaatgctcatgttgttcatgctgatcttagcgagtatggtgcaatggatgagaagtgtattcacttagctgagctagcagcaactgccgtggacttccccaagactggcaaacttgcgataatgccaccacaccttaaaccaaaagtctaccctgacttcatgggaaaggaagatggacaatcttataaatcagaaaagattcttggaaggctttatcgttcaatccaagaggcctccaatggtgatgtggtgtcacaagaggtttgcactccaaatgatctgccttatgacatagatctggaagttcctggtgcatcagatttcctcgcgagtgcttggcaatgcaagtgttcatacgacgcgcagctgagtgcgctgctcagtcagtacagggtccgcactgaagctgaacttgtgacagggcacataacgttccttgttaagaacagcagcaagaagcaaggcgacataaaggacaggctgaagactgcttactctgcactacgcaaggagttcaaaagtacctttgagagcatagcatcggatcaatgcgagattggtgatgacgagaagaatctgctgtacgagatgaaggcctctgcctggtaccaggtgacctatcaccccaaatgggtggagaagtcgaggggcattctgggcccagatggtgaggaaataccggcaagccttagctttgcatggatcccggtggattacttggcgcggataaagctaaggtgccatggtaaagtgagagttgaaggccaaaagcctgttgaaaggcttgcagcatacatctccgagaggatatgatgaaacaatgcaaaggtcgcagtaagaccactctgccattcgtaatgccggttcaaagggcatgcgtacaaaatatctgagtttttttttcttttcatttttgttcacttcactgaacttgatctcccgaatgtttcagtgtgcttttgttccttcttacatgcccctcaagcctgaaaaactgtacgtttcagttgagggctgtctatattatgaaatgcacaaatatacgctgcctgcagcttttggcaat</dnaseqindica>|
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Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001049764.1 RefSeq:Os01g0527600]|
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==Labs working on this gene==
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}}
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* State Key Laboratory of Plant Genomics and National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China
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[[Category:Genes]]
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* Crop Molecular Breeding Center, the Institute of Crop and Nuclear Technology Utilization, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
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[[Category:Japonica mRNA]]
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* State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
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[[Category:Oryza Sativa Japonica Group]]
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==References==
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[[Category:Japonica Genes]]
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<references>
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[[Category:Japonica Chromosome 1]]
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* <ref name="ref1">
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[[Category:Chromosome 1]]
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Song X, Wang D, Ma L, Chen Z, Li P, Cui X, Liu C, Cao S, Chu C, Tao Y, Cao X.  
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Rice RNA-dependent RNA polymerase 6 acts in small RNA biogenesis and spikelet
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development. Plant J. 2012 Aug;71(3):378-89. doi:
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10.1111/j.1365-313X.2012.05001.x. Epub 2012 Jun 5. PubMed PMID: 22443269.
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</ref>
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</references>
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==Structured Information==
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    [[Category:Genes]][[Category:Oryza Sativa Japonica Group]][[Category:Japonica Chromosome 1]]

Latest revision as of 04:45, 12 October 2016

The rice Os01g0527600 was reported as OsRDR6 in 2012 [1] by researchers from China.

Annotated Information

Figure 1. Characterization of the rice temperature-sensitive mutant osrdr6-1.[1].

Gene Symbol

  • Os11g0267000 <=> OsRDR6,OsSHL2,RDR6,SHL2

Function

  • The rice OsRDR6 acts in the biogenesis of various types and sizes of small RNAs.
  • OsRDR6-dependent tasiR-ARFs play key roles in lemma development in rice.
  • OsRDR6 is required for the biogenesis of 24-nt phased small RNAs.
  • OsRDR6-dependent 24-nt phased small RNAs are independent of the RNA-directed DNA methylation (RdDM) pathway.
  • OsRDR6 mediates secondary small RNA biogenesis in rice.

Phenotypic analysis

  • The researchers identified a spontaneous rice mutant with pleiotropic spikelet defects, with most spikelets showing needle-shaped, awn-like lemma; this phenotype is similar to that of shl2-rol and osdcl4-1 mutants (Liu et al., 2007; Nogueira et al., 2007; Toriba et al., 2010).
  • Unlike the con- sistent spikelet defects in shl2-rol and osdcl4-1, the osrdr6-1 mutants showed various degrees of spikelet defects under different natural growth conditions.
  • The spikelet defects and altered stamen number of the mutant only occurred at high temperatures and under long-day conditions, as found in July in Hangzhou. Mutant plants were normal when grown in low temperatures and short-day conditions, as found in October at the same location .
  • The developmental defects of osrdr6-1 were rescued by a construct containing the full-length OsRDR6 genomic DNA; the transgenic plants ap- peared morphologically identical to wild-type Zhonghua11 (Figure 1b), confirming that the OsRDR6 locus is responsible for the mutant phenotype.


You can also add sub-section(s) at will.

Labs working on this gene

  • State Key Laboratory of Plant Genomics and National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China
  • Crop Molecular Breeding Center, the Institute of Crop and Nuclear Technology Utilization, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
  • State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China

References

  1. ↑ 1.0 1.1 Song X, Wang D, Ma L, Chen Z, Li P, Cui X, Liu C, Cao S, Chu C, Tao Y, Cao X. Rice RNA-dependent RNA polymerase 6 acts in small RNA biogenesis and spikelet development. Plant J. 2012 Aug;71(3):378-89. doi: 10.1111/j.1365-313X.2012.05001.x. Epub 2012 Jun 5. PubMed PMID: 22443269.

Structured Information