Difference between revisions of "Os02g0606000"
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==Annotated Information== | ==Annotated Information== | ||
| + | [[File:Os02g0606000.png|right|thumb|427px|'''Figure 1.''' ''Characterization of the osdg2 mutants.<ref name="ref1" />.'']] | ||
===Function=== | ===Function=== | ||
* '''''OsDG2''''' gene encoded a novel chloroplast-targeted GRP in rice. | * '''''OsDG2''''' gene encoded a novel chloroplast-targeted GRP in rice. | ||
* Disruption of the '''''OsDG2''''' would lead to delayed greening phenotype and affected expression levels of genes associated with chloroplast development at early leaf stage of rice. | * Disruption of the '''''OsDG2''''' would lead to delayed greening phenotype and affected expression levels of genes associated with chloroplast development at early leaf stage of rice. | ||
| + | |||
| + | ===Phenotypic analysis=== | ||
| + | * Leaves of the osdg2 mutant displaying a delayed greening phenotype were characterized with bright yellow until three-leaf stage, thereafter, the emerging leaves turned to green (Fig. 1A). | ||
| + | * Likewise, the contents of Chl a, b, and Car for the 3rd leaves were significantly reduced in osdg2 mutant (Fig. 1B), but those for the 4th leaves reached a normal level as WT Jiahua 1 (Fig. 1D). | ||
| + | * This result is in accordance with the leaf phenotype of osdg2 mutant. | ||
===Expression=== | ===Expression=== | ||
| − | * | + | * RT-PCR analysis showed that the '''''OsDG2''''' transcripts were highly expressed in leaves and undetectable in other tissues, showing the tissue-specific expression. |
===Subcellular localization=== | ===Subcellular localization=== | ||
Latest revision as of 05:49, 12 October 2016
The rice Os02g0606000 was reported as OsDG2 in 2013 [1] by researchers from China.
Contents
Annotated Information
Figure 1. Characterization of the osdg2 mutants.[1].
Function
- OsDG2 gene encoded a novel chloroplast-targeted GRP in rice.
- Disruption of the OsDG2 would lead to delayed greening phenotype and affected expression levels of genes associated with chloroplast development at early leaf stage of rice.
Phenotypic analysis
- Leaves of the osdg2 mutant displaying a delayed greening phenotype were characterized with bright yellow until three-leaf stage, thereafter, the emerging leaves turned to green (Fig. 1A).
- Likewise, the contents of Chl a, b, and Car for the 3rd leaves were significantly reduced in osdg2 mutant (Fig. 1B), but those for the 4th leaves reached a normal level as WT Jiahua 1 (Fig. 1D).
- This result is in accordance with the leaf phenotype of osdg2 mutant.
Expression
- RT-PCR analysis showed that the OsDG2 transcripts were highly expressed in leaves and undetectable in other tissues, showing the tissue-specific expression.
Subcellular localization
- Subcellular localization results showed that OsDG2 was targeted in chloroplasts.
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Labs working on this gene
- The Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, 12 South Zhong-Guan Cun Street, Beijing 100081, China
References
- ↑ 1.0 1.1 Jiang Q, Ma X, Gong X, Zhang J, Teng S, Xu J, Lin D, Dong Y. The rice OsDG2 encoding a glycine-rich protein is involved in the regulation of chloroplast development during early seedling stage. Plant Cell Rep. 2014 May;33(5):733-44. doi: 10.1007/s00299-013-1549-4. Epub 2014 Jan 16. PubMed PMID: 24430865.
