Difference between revisions of "Os10g0409400"
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* Mechanical strength measurement of '''''Ubi::OsBURP16''''' plants showed that reduced force was required to break leaves as compared with wild type. | * Mechanical strength measurement of '''''Ubi::OsBURP16''''' plants showed that reduced force was required to break leaves as compared with wild type. | ||
* Transgenic rice showed enhanced PG activity and reduced pectin content. | * Transgenic rice showed enhanced PG activity and reduced pectin content. | ||
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[[File:59-Os10g0409400.png|center|thumb|727px|'''Figure 2.''' ''Generation of Ubi::OsBURP16 transgenic rice..<ref name="ref1" />.'']] | [[File:59-Os10g0409400.png|center|thumb|727px|'''Figure 2.''' ''Generation of Ubi::OsBURP16 transgenic rice..<ref name="ref1" />.'']] | ||
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===Expression=== | ===Expression=== | ||
* RT-PCR (qRT-PCR) assays showed '''''OsBURP16''''' was expressed in various tissues, mainly in leaf lamina, leaf cushion and internodes (Fig. 1c). These results show that OsBURP16 is highly expressed in young leaves. | * RT-PCR (qRT-PCR) assays showed '''''OsBURP16''''' was expressed in various tissues, mainly in leaf lamina, leaf cushion and internodes (Fig. 1c). These results show that OsBURP16 is highly expressed in young leaves. | ||
Revision as of 09:40, 12 October 2016
The rice Os10g0409400 was reported as OsBURP16 in 2014 [1] by researchers from China.
Contents
Annotated Information
Function
- Polygalacturonase (PG), one of the hydrolases responsible for cell wall pectin degradation, is involved in organ consenescence and biotic stress in plants.
- OsBURP16 belongs to the PG1β-like subfamily of BURP- family genes and encodes one putative PG1β subunit precursor in rice (Oryza sativa L.).
- Overexpression of OsBURP16 caused pectin degradation and affected cell wall integrity as well as transpiration rate, which decreased tolerance to abiotic stresses.
Phenotypic analysis
- Analysis of plant survival rates, relative ion leakage rates, accumulation levels of H2O2 and water loss rates of leaves showed that overexpression of OsBURP16 enhanced sensitivity to cold, salinity and drought stresses compared with controls.
- Young leaves of Ubi::OsBURP16 transgenic plants showed reduced cell adhesion and increased cuticular transpiration rate.
- Mechanical strength measurement of Ubi::OsBURP16 plants showed that reduced force was required to break leaves as compared with wild type.
- Transgenic rice showed enhanced PG activity and reduced pectin content.
Figure 2. Generation of Ubi::OsBURP16 transgenic rice..[1].
Expression
- RT-PCR (qRT-PCR) assays showed OsBURP16 was expressed in various tissues, mainly in leaf lamina, leaf cushion and internodes (Fig. 1c). These results show that OsBURP16 is highly expressed in young leaves.
- Transcription of OsBURP16 is induced by cold, salinity and drought stresses, as well as by abscisic acid (ABA) treatment.
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Labs working on this gene
- Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China
- Graduate University of the Chinese Academy of Sciences, Beijing 100049, China
References
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