Difference between revisions of "Os03g0150800"

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(Created page with "{{JaponicaGene| GeneName = Os03g0150800| Description = High affinity phosphate transporter 2 (Phosphate transporter)| Version = NM_001055514.1 GI:115450756 GeneID:4331637| ...")
 
(Expression)
 
(6 intermediate revisions by 3 users not shown)
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{{JaponicaGene|
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The rice '''Os03g0150800''' was reported as ''' OsPT2''' in 2008 <ref name="ref1" /> by researchers from the China.
GeneName = Os03g0150800|
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<br>
Description = High affinity phosphate transporter 2 (Phosphate transporter)|
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==Annotated Information==
Version = NM_001055514.1 GI:115450756 GeneID:4331637|
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===Gene Symbol===
Length = 1674 bp|
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*''' Os03g0150800 <=> OsPT2'''
Definition = Oryza sativa Japonica Group Os03g0150800, complete gene.|
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<br>
Source = Oryza sativa Japonica Group
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===Function===
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*OsPT2 is a low-affinity Pi transporter,and functions in translocation of the stored Pi in the plant.
 +
<br>
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===Expression===
 +
*Expression of OsPht1;2 (OsPT2) and OsPht1;6 (OsPT6) genes increased significantly under Pi deprivation in roots and shoots. By using transgenic rice plants expressing the GUS reporter gene, driven by their promoters, we detected that OsPT2 was localized exclusively in the stele of primary and lateral roots.
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*Xenopus oocytes injected with OsPT2 mRNA showed increased Pi accumulation and a Pi-elicited depolarization of the cell membrane electrical potential, when supplied with mM external concentrations.  
 +
<br>
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[[File:46.png |center |thumb |10000px |''''''Figure 2. Localization of OsPT2 promoter-GUS expression in transformed rice.'''''' ]]
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<br>
  
  ORGANISM  Oryza sativa Japonica Group
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===Mutation===
            Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
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*The knock-down of either OsPT2 or OsPT6 expression by RNA interference significantly decreased both the uptake and the long-distance transport of Pi from roots to shoots.
            Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
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<br>
            clade; Ehrhartoideae; Oryzeae; Oryza.
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==Labs working on this gene==
|
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*State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Resources and Environmental Sciences,Nanjing Agricultural University, Nanjing 210095, China,
Chromosome = [[:category:Japonica Chromosome 3|Chromosome 3]]|
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*The Center for Cell and Molecular Signaling, School of Medicine, Emory University, 30322 USA,
AP = Chromosome 3:2794030..2795703|
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*State Key Laboratory of Plant Physiology and Biochemistry, College of Life Science, Zhejiang University, Hangzhou 310029,China,
CDS = 2794072..2795658|
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*Centre for Soils and Ecosystem Function, Rothamsted Research, Harpenden, Hertfordshire AL5 2JQ, UK
GCID = <gbrowseImage1>
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<br>
name=NC_008396:2794030..2795703
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==References==
source=RiceChromosome03
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<references>
preset=GeneLocation
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<ref name="ref1">
</gbrowseImage1>|
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Characterization of a histidine- and alanine-rich protein showing
GSID = <gbrowseImage2>
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name=NC_008396:2794030..2795703
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interaction with calreticulin in rice
source=RiceChromosome03
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</ref>
preset=GeneLocation
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</gbrowseImage2>|
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</references>
CDNA = <cdnaseq>atggcgggatcgcagctcaacgttttggtcaagctcgaccaggccaagacgcaatggtaccatttcatggcgatcgtcatcgccgggatggggttcttcacagacgcctacgacctcttttgcatcgcgctggtcaccaagctgttgggtcgcctttactacaccgacatcaccaagcccaacccagggacactcccgcccaacgtgtcgtccgccgtcaccggcgtcgcgctctgcggcacgctcgccggccagctcttcttcggatggctcggcgacaagctcggccgcaagagcgtctatggattcacgctcatcctcatggtcgtgtgctccatcgcctccggtctctcgtttggccacacgcccaagagcgtgattgccacgctctgcttcttccgcttctggctcggcttcggcatcggcggcgactacccgctcagcgccaccatcatgtcggagtacgcgagcaagaagacccgcggggccttcatcgccgccgtgttcgccatgcaggggttcgggatcctcttcggcgccatcgtcgcgctcgtcgtctcggccggcttccgtcacgcgtacccggcgccgtcgtacgcccagaaccccgccgcgtcgctcgcgccgcaggctgactacacgtggcggctcatcctcatgttcggcaccatcccggctgggctcacctactactggcgcatgaaaatgcccgagacggcgcggtacacggcgctcgtcgcccgcaacgccaagcaggcggcggctgacatgtccaaggtgctccacgccgagatcgaggagcggccggaggtggtcgagagccaggtggtcgccggggagacctggggcctcttctcacggcagttcatgaagcgccacgggatgcacctcctggcgaccaccagcacgtggttcctgctcgacatcgccttctacagccagaacctgttccagaaggacatcttcagcaaggtcgggtggataccgccggcgaagaccatgaacgcgctcgaggaactctaccgcatctcccgcgcccaggcactcatcgcactctgcggaaccatcccgggctactggttcaccgtcgccttcatcgacatcgtcggcaggttctggatccagatcatgggcttcttcatgatgaccgtgttcatgctcgcgctcggcgtgccctacgaccactggacgcacccggcgcaccacaccggcttcgtcgtgctctacgcactcaccttcttcttcgccaacttcgggcccaacagcaccaccttcatcgtgccggctgagatcttcccggcgaggctccggtcgacgtgccacggcatctccgccgcgtccggcaaggccggcgcgatcatcggcgcgttcgggttcctgtacgctgcgcaggaccagcacaatcccgacgcgggatactcccgcggcatcggcatccggaacgcgctcttcgtgctcgccggcacaaacttcctcggtatgctcatgacgctgctggtgccggagtccaagggcttgtcgctggaggagatgtccaaggacaacgtcgtcgacgagaccgcccaagaagcgatcgcccaagcgtga</cdnaseq>|
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<br>
AA = <aaseq>MAGSQLNVLVKLDQAKTQWYHFMAIVIAGMGFFTDAYDLFCIAL                    VTKLLGRLYYTDITKPNPGTLPPNVSSAVTGVALCGTLAGQLFFGWLGDKLGRKSVYG                    FTLILMVVCSIASGLSFGHTPKSVIATLCFFRFWLGFGIGGDYPLSATIMSEYASKKT                    RGAFIAAVFAMQGFGILFGAIVALVVSAGFRHAYPAPSYAQNPAASLAPQADYTWRLI                    LMFGTIPAGLTYYWRMKMPETARYTALVARNAKQAAADMSKVLHAEIEERPEVVESQV                    VAGETWGLFSRQFMKRHGMHLLATTSTWFLLDIAFYSQNLFQKDIFSKVGWIPPAKTM                    NALEELYRISRAQALIALCGTIPGYWFTVAFIDIVGRFWIQIMGFFMMTVFMLALGVP                    YDHWTHPAHHTGFVVLYALTFFFANFGPNSTTFIVPAEIFPARLRSTCHGISAASGKA                    GAIIGAFGFLYAAQDQHNPDAGYSRGIGIRNALFVLAGTNFLGMLMTLLVPESKGLSL                    EEMSKDNVVDETAQEAIAQA</aaseq>|
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==Structured Information==
DNA = <dnaseqindica>46..1632#ctaagcaaagcttataactttgcagcttgaggaaaaaagatcatcatggcgggatcgcagctcaacgttttggtcaagctcgaccaggccaagacgcaatggtaccatttcatggcgatcgtcatcgccgggatggggttcttcacagacgcctacgacctcttttgcatcgcgctggtcaccaagctgttgggtcgcctttactacaccgacatcaccaagcccaacccagggacactcccgcccaacgtgtcgtccgccgtcaccggcgtcgcgctctgcggcacgctcgccggccagctcttcttcggatggctcggcgacaagctcggccgcaagagcgtctatggattcacgctcatcctcatggtcgtgtgctccatcgcctccggtctctcgtttggccacacgcccaagagcgtgattgccacgctctgcttcttccgcttctggctcggcttcggcatcggcggcgactacccgctcagcgccaccatcatgtcggagtacgcgagcaagaagacccgcggggccttcatcgccgccgtgttcgccatgcaggggttcgggatcctcttcggcgccatcgtcgcgctcgtcgtctcggccggcttccgtcacgcgtacccggcgccgtcgtacgcccagaaccccgccgcgtcgctcgcgccgcaggctgactacacgtggcggctcatcctcatgttcggcaccatcccggctgggctcacctactactggcgcatgaaaatgcccgagacggcgcggtacacggcgctcgtcgcccgcaacgccaagcaggcggcggctgacatgtccaaggtgctccacgccgagatcgaggagcggccggaggtggtcgagagccaggtggtcgccggggagacctggggcctcttctcacggcagttcatgaagcgccacgggatgcacctcctggcgaccaccagcacgtggttcctgctcgacatcgccttctacagccagaacctgttccagaaggacatcttcagcaaggtcgggtggataccgccggcgaagaccatgaacgcgctcgaggaactctaccgcatctcccgcgcccaggcactcatcgcactctgcggaaccatcccgggctactggttcaccgtcgccttcatcgacatcgtcggcaggttctggatccagatcatgggcttcttcatgatgaccgtgttcatgctcgcgctcggcgtgccctacgaccactggacgcacccggcgcaccacaccggcttcgtcgtgctctacgcactcaccttcttcttcgccaacttcgggcccaacagcaccaccttcatcgtgccggctgagatcttcccggcgaggctccggtcgacgtgccacggcatctccgccgcgtccggcaaggccggcgcgatcatcggcgcgttcgggttcctgtacgctgcgcaggaccagcacaatcccgacgcgggatactcccgcggcatcggcatccggaacgcgctcttcgtgctcgccggcacaaacttcctcggtatgctcatgacgctgctggtgccggagtccaagggcttgtcgctggaggagatgtccaaggacaacgtcgtcgacgagaccgcccaagaagcgatcgcccaagcgtgatgtcataaacatgccgtctcgacgtgagtgactgaaaaaaaa</dnaseqindica>|
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    [[Category:Genes]][[Category:Oryza Sativa Japonica Group]][[Category:Japonica Chromosome 3]]
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001055514.1 RefSeq:Os03g0150800]|
 
}}
 
[[Category:Genes]]
 
[[Category:Japonica mRNA]]
 
[[Category:Oryza Sativa Japonica Group]]
 
[[Category:Japonica Genes]]
 
[[Category:Japonica Chromosome 3]]
 
[[Category:Chromosome 3]]
 

Latest revision as of 02:48, 16 October 2016

The rice Os03g0150800 was reported as OsPT2 in 2008 [1] by researchers from the China.

Annotated Information

Gene Symbol

  • Os03g0150800 <=> OsPT2


Function

  • OsPT2 is a low-affinity Pi transporter,and functions in translocation of the stored Pi in the plant.


Expression

  • Expression of OsPht1;2 (OsPT2) and OsPht1;6 (OsPT6) genes increased significantly under Pi deprivation in roots and shoots. By using transgenic rice plants expressing the GUS reporter gene, driven by their promoters, we detected that OsPT2 was localized exclusively in the stele of primary and lateral roots.
  • Xenopus oocytes injected with OsPT2 mRNA showed increased Pi accumulation and a Pi-elicited depolarization of the cell membrane electrical potential, when supplied with mM external concentrations.


'Figure 2. Localization of OsPT2 promoter-GUS expression in transformed rice.'


Mutation

  • The knock-down of either OsPT2 or OsPT6 expression by RNA interference significantly decreased both the uptake and the long-distance transport of Pi from roots to shoots.


Labs working on this gene

  • State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Resources and Environmental Sciences,Nanjing Agricultural University, Nanjing 210095, China,
  • The Center for Cell and Molecular Signaling, School of Medicine, Emory University, 30322 USA,
  • State Key Laboratory of Plant Physiology and Biochemistry, College of Life Science, Zhejiang University, Hangzhou 310029,China,
  • Centre for Soils and Ecosystem Function, Rothamsted Research, Harpenden, Hertfordshire AL5 2JQ, UK


References

  1. Characterization of a histidine- and alanine-rich protein showing interaction with calreticulin in rice


Structured Information