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| − | {{JaponicaGene|
| + | The rice gene Os05g0405000 was reported as ''''' flo4''''' in 2005<ref name="ref1" /> by researchers from Korea and Japan. |
| − | GeneName = Os05g0405000|
| |
| − | Description = Orthophosphate dikinase precursor (EC 2.7.9.1)|
| |
| − | Version = NM_001062042.1 GI:115463814 GeneID:4338750|
| |
| − | Length = 19162 bp|
| |
| − | Definition = Oryza sativa Japonica Group Os05g0405000, complete gene.|
| |
| − | Source = Oryza sativa Japonica Group
| |
| | | | |
| − | ORGANISM Oryza sativa Japonica Group
| + | ==Annotated Information== |
| − | Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
| + | ===Gene Symbol=== |
| − | Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
| + | *'''''Os05g0405000 <=> flo4, flo4-2, flo4-3, ppdk, OsPPDK, PPDK1, CPDK1, PPDKB, OsPPDKB, cyOsPPDKB, OsPBDK, PBDK, osppdkb |
| − | clade; Ehrhartoideae; Oryzeae; Oryza.
| + | |
| − | |
| + | ===Function=== |
| − | Chromosome = [[:category:Japonica Chromosome 5|Chromosome 5]]|
| + | * '''''OsPPDKB''''' gene encodes pyruvate orthophosphate dikinase (PPDK). |
| − | AP = Chromosome 5:19725353..19744514|
| + | * Cytosolic PPDK functions in rice to modulate carbon metabolism during grain filling. |
| − | CDS = 19725598..19725640,19725722..19725813,19725901..19725978,19726153..19726290,19726536..19726664<br>,19726947..19727076,19727229..19727406,19727773..19727911,19728089..19728178<br>,19728318..19728479,19728614..19728778,19728967..19729104,19729715..19729894<br>,19730187..19730315,19730500..19730735,19730853..19731015,19732474..19732611<br>,19732728..19732961,19733067..19733123,19743931..19744155|
| + | |
| − | GCID = <gbrowseImage1>
| + | ===Mutation=== |
| − | name=NC_008398:19725353..19744514
| + | [[File:Os05g0405000-1.png|right|thumb|250px|'''Figure 1. Phenotypes of flo4 kernels.''' '' <ref name="ref1" />.'']] |
| − | source=RiceChromosome05
| + | * The researchers conducted a screening for GUS activity to identify knockout mutations in the genes expressed in mature seeds. Among the several hundred GUS-positive lines that were identified, we found one in which the white-core endosperm mutant phenotype co-segregated. This floury mutation was recessively segregated (Figure 1a), and was named floury-4 (flo4). |
| − | preset=GeneLocation
| + | |
| − | </gbrowseImage1>| | + | * Cross-sectioning revealed that the interior of the endosperm was floury-white while the outer portion was normal (Figure 1d). This suggests that starch accumulation was abnormal in the early stages of ripening, but recovered later on in development. The scanning electron microscopic analysis showed that the white endosperm comprised round and loosely packed, compound starch granules (Figure 1f). |
| − | GSID = <gbrowseImage2>
| + | |
| − | name=NC_008398:19725353..19744514
| + | |
| − | source=RiceChromosome05
| + | |
| − | preset=GeneLocation
| + | |
| − | </gbrowseImage2>| | + | |
| − | CDNA = <cdnaseq>atgccgtcggtttcgagggccgtgtgcgtgcagagggcgagcgggaataacggcaggaggtgcagagatggggcggccgccgccggccgccgatccgtcgtggcgcagagagcgcggcacggcaagccggaggtcgccatccgctcggggtccggcggctcggcgcggggcgggcattgctcgcctctcagggcagtggcggcgccgatcccgactaccaaaaagagggtgttccacttcggcaagggcaagagcgagggcaacaaggccatgaaggacctgctgggagggaagggggcgaacttggcggagatggcgagcatcgggctgtcggtgccgccggggttcacggtgtcgacggaggcgtgccagcagtaccaggcggcggggaagacgctgccggcggggctgtgggaggagatcgtcgaggggctccagtgggtggaggagtacatggcggcgcgcctcggcgaccccgcccgccctctcctcctctccgtccgctccggcgccgccgtgtcgatgccggggatgatggacacggtgctgaacctggggctgaacgacgaggtggcggcgggactggcggccaagagcggcgaccgcttcgcgtacgactcctaccgccgtttcctcgacatgttcggcaacgtggtcatggacattcctcatgcactgttcgaggagaagctcgaagccatgaaagcagtcaaggggctgcacaacgacactgacctgactgccactgacctcaaagaactagtggcacagtacaaggatgtctatgttgaagctaagggagagccatttccctctgatcccaagaagcaactgcagctggccgtgttggccgtgttcaactcatgggatagcccaagagcaatcaagtacagaagcataaacaagatcactgggctgaagggcactgctgtaaacgtgcagaccatggtgtttggaaacatggggaacacctctggcacgggtgtgctcttcactaggaacccaagcactggagagaagaagctttacggcgaattccttgtgaatgctcagggtgaagatgtggttgctggaatcagaactccagaggatcttgatgctatgagggaccacatgccggagccttatgaagagctcgttgagaactgcaaaattttggagagtcactataaagaaatgatggatattgaatttactgttcaggaaaataggctttggatgcttcagtgcagaacaggaaagcgcacaggaaaaggtgctgtaaagattgctgtagacatggttaacgagggccttgttgagcgccgcacagcacttaagatggtagaaccaggtcacctggatcagcttcttcatcctcagtttgagaacccatcagggtataaagacaaagttattgccacgggcttacctgcatcacctggggctgctgtgggccaaattgtattcactgccgaggatgctgaagcatggcatgcccaagggaaagatgttattctggtgaggacagagaccagcccagaggatgttggtggcatgcatgcagctgttggaattcttacagcaagaggtggtatgacctctcacgctgctgttgttgcgcgcggatggggcaaatgttgtgtgtcaggatgctcaagcgtccgtgtaaatgatgcgtccaagattgtagtgattgaagacaaggcgctgcatgaaggtgagtggctatcgttgaatggatcaactggtgaagtgatcattggcaagcagccactctgcccaccagcccttagtggtgatttggaaactttcatgtcttgggtggatgaagttaggaaactcaaggttatggcaaatgctgatacccccgaggatgcaacaacagccagacagaatggggcagaaggaattgggctatgccggactgaacatatgttcttcgcttcagacgagaggattaaggctgtcaggcagatgattatggcttcaagtcttgaactgaggcagaaagcactagatcgccttttgccttatcagaggtctgactttgaaggcatcttccgtgcaatggatgggcttccggtaactattagactcttggatcctccacttcatgagttccttccagaaggccatgttgaggatatggtgcgtgagctatgctctgaaactggagcagctcaggatgatgtccttgcaagagtagaaaaactttccgaagtaaatccaatgcttggtttccgtgggtgcaggcttggtatatcataccctgaattgactgaaatgcaagcccgtgccatctttgaagctgctataaccatgaccaaccagggtattcaagtctttccggagataatggttccccttgttggaactcctcaggaattggggcatcaagtggatgttatccgtcaaattgccaacaaggtgttcacagacatgggaaaaactattggctacaaagttggaactatgattgaaattcccagggcagctttagtggctgatgagatagcagagcaggctgagttcttctcctttggaacaaacgacctaacacagatgacatttggttacagtagggatgatgtggggaagttccttcccatctatctgtctcagggtatcctccagcatgacccctttgaggtgcttgatcagaggggagttggggagctggttaagctggctacagagagaggccgcaaagctaggcctaacttgaaggtgggcatctgtggtgaacatggtggagagcctctgtcagtcgctttctttgcgaaggctgggctggactatgtttcttgttcgcctttcagggtcccaattgctaggctagctgcagctcaggtgctcctctga</cdnaseq>|
| + | |
| − | AA = <aaseq>MPSVSRAVCVQRASGNNGRRCRDGAAAAGRRSVVAQRARHGKPE VAIRSGSGGSARGGHCSPLRAVAAPIPTTKKRVFHFGKGKSEGNKAMKDLLGGKGANL AEMASIGLSVPPGFTVSTEACQQYQAAGKTLPAGLWEEIVEGLQWVEEYMAARLGDPA RPLLLSVRSGAAVSMPGMMDTVLNLGLNDEVAAGLAAKSGDRFAYDSYRRFLDMFGNV VMDIPHALFEEKLEAMKAVKGLHNDTDLTATDLKELVAQYKDVYVEAKGEPFPSDPKK QLQLAVLAVFNSWDSPRAIKYRSINKITGLKGTAVNVQTMVFGNMGNTSGTGVLFTRN PSTGEKKLYGEFLVNAQGEDVVAGIRTPEDLDAMRDHMPEPYEELVENCKILESHYKE MMDIEFTVQENRLWMLQCRTGKRTGKGAVKIAVDMVNEGLVERRTALKMVEPGHLDQL LHPQFENPSGYKDKVIATGLPASPGAAVGQIVFTAEDAEAWHAQGKDVILVRTETSPE DVGGMHAAVGILTARGGMTSHAAVVARGWGKCCVSGCSSVRVNDASKIVVIEDKALHE GEWLSLNGSTGEVIIGKQPLCPPALSGDLETFMSWVDEVRKLKVMANADTPEDATTAR QNGAEGIGLCRTEHMFFASDERIKAVRQMIMASSLELRQKALDRLLPYQRSDFEGIFR AMDGLPVTIRLLDPPLHEFLPEGHVEDMVRELCSETGAAQDDVLARVEKLSEVNPMLG FRGCRLGISYPELTEMQARAIFEAAITMTNQGIQVFPEIMVPLVGTPQELGHQVDVIR QIANKVFTDMGKTIGYKVGTMIEIPRAALVADEIAEQAEFFSFGTNDLTQMTFGYSRD DVGKFLPIYLSQGILQHDPFEVLDQRGVGELVKLATERGRKARPNLKVGICGEHGGEP LSVAFFAKAGLDYVSCSPFRVPIARLAAAQVLL</aaseq>|
| + | |
| − | DNA = <dnaseqindica>18875..18917#18702..18793#18537..18614#18225..18362#17851..17979#17439..17568#17109..17286#16604..16742#16337..16426#16036..16197#15737..15901#15411..15548#14621..14800#14200..14328#13780..14015#13500..13662#11904..12041#11554..11787#11392..11448#360..584#cgattggggcgcttgtgaacaccggggcgccacgtggagaggacgttacactccagccgccaaatttccactcccacacccgcgctcccctcccctctcttttccgtgatcgcacctcgcccacgcgccccccgccacacacaatctctgcagctctccagcttcgttggaactcgcgaatctctctccgatcccaggtaaagcagcgaacgacgtcacgcacgacgctgctcggtggatttcgttccttgctggggaaaaccatgcagagacgaaggtgaatgatctgcttttgtgtacttgcgtttaccaggtgaagcgcgagcttggagttggaggggagatcgatcagggccaggatgccgtcggtttcgagggccgtgtgcgtgcagagggcgagcgggaataacggcaggaggtgcagagatggggcggccgccgccggccgccgatccgtcgtggcgcagagagcgcggcacggcaagccggaggtcgccatccgctcggggtccggcggctcggcgcggggcgggcattgctcgcctctcagggcagtggcggcgccgatcccgactaccaaaaaggtaaaccgtgcatgccgcagctcctcgttcgttcgttggctcgcgcgcgcgattgctgtgtttgacacaccgttgactctcgatcgtgcgagcatctatcactcactggttcgactcgcatgcgtattgacgtataggagctcggaacacaccatcgtatagctcacgaggtagcactgtagcggggatatgcgtggttggccagcccattgagccacataaggcccatttttttctacacacttgaattcaatccggccctatggttggcactgtctctcatttatttatttactcctttatttagaggatgcgcagatgcatgcgttcaacatcttttagggacggagtacatatttagattcacacgtgtgatattggaagtgcgggcttgtgtgacgctgtatccttgcactataaaaaaacatctctgctactagtaggtcataaccggcatcattgttggtttttaaactggcagtgtgctaccgacaatgatacgattggtttagggtgtgaaccaatagtgatatatggagcataactgttaattctacttacaaacaaacatacagtgattttgaacgctccaaaaatatatatgcaatgccaatagatttgttgatttgcattcaccgatccaacaaacaacttcatttcattcatcaatctaacaatcaaaagtttgtaacaaattcacttcatttcaccaatccaacaatgtggtttcattgattgatcaacaatagaataaagtgtaactttggtgtgatgaacaacagctagcagaacaatttctttccagaaaatttttattgcatcatgcagcttctaaaaatagataaaagattgctcatgcatctcttctcctcatcaccttcaaagatgtagaaggtagcagaggagcacatcggttgataataatgtagatggcactgttagttgatgttgttgagcttgtgtccctgttggcaaatcaagtaagcacaacccccctcccccccaccctccataggatttggtccaagcttgggatgaccagatataattccacatagcctcatctctatcgcatgttgttgttaaacataccatgcgacgtccgcctccattgctttgtaggtatttgccgcgttggacagggaccgatgtcatcgagatccactcttaatcctgagagtatgaaggcatggaagggggcctccaccaccacctgcctaacgagattgaggtggtggcctatgggctagatccccctccccccttccaagcagcaagctcgtggcatgctatccgatcaaaggggagcaaggcggtggccccttgttcggagagtaaggaagtgacggcacatcagttggagggtagagagacggcacactgtgcatgaggagagagggagggaaaataccaacagtggtactatcattatcaatttatatcctaaattatttgtaaaaagagtacaactacatgttctaaatgagataaacctgcagatattggaaatgatgcatttaggtccacatgatgtgatgatagttgttctttgttcagtttggttagcatacagatagatatcgacaatatttttttaaagataatgaaagctacagccaattatacatacagaagttgctagtcaaatcaccgcaaattacaagatcacagcttgacaaaaacaagaaagcaacttagaatatcaacctaaggacatacacataaaacacctattgctaaaattcatttatgattaacaagaatctgtataaccaagggtagagatgggagaagactgctagggctgcaaccctactcttaagtctaaatatccattagaattgcactataaaattaaaaaaaaaatgggacaaagttatgtaaattatattagctgagccctaagcttagaagaattctggttctaccatagcataaccatttgttttggctaccaatgactgtttataaagattatcatcgattagggaaaaaggaaacaaaagtatgcaaataaatgataataaatcaactctctagttgagtagctaggctcataggtgcaaactgctgaaagcttaatcattccatcatttggaatcttgggggtaagacaaactgtaatgactctatctatatagagctagtgactcccaatcaatttggtaggctttctagttaccttagatatttctactttttatcatgcacttcacagaggcatctcagattctggattgccaagcccatggtttcaccaaaaccagaaaaacagatacagttatatgcaaagattttttttttcatcagtcccagactcccagtacatcaagaatgtgaacggaatcgagcctggaatccatggtgagacagggaccgaattcatcttagcaatctagcaacttgattaagcatttaagccggtaagctcgaccattaggcaagtggacaaggttgtgatcacgcaagtaaataaaaactataggatttaagtacttttcgccgcggctaaatccaagtgatcacgggccctttgacagcaatctagccaattagcacggaggtgtatcaacacatgcatgaatggaatatatttctccctggtgcagagaggttcatttcacttggcatcatcacggacctaagcaaacattaggggcatatgctacgaaattcttgtctgtccatcaaatgtttggtagcaataatattcccatgttcactcgtgcatattgctaattttgttgttttgttgacgtttaactcaaatcatggtttctataagttacttcatggtattttgaggaactaccaaatcatggtgaatcatatatgatcgaactaccaaatcggccgacagtgcaatatacatggttctgttctctggttttgcccctagttcagcccatttctagttagggcataaggtgttttcttttaaaagaactaggtgattctccgcgctttgctgcgggaatactaagcaatattccgacaatcatgctaggtgtaaaaaaaaatgaaatatgatggtttattagtacttatcataaaacaaacaaatgataaatgtgatttaatttaatgtagaacatattgataattatatgttaaatattacaaaaatgatagatataattgttaaaatattgtgaaaatgatgtaaggggtgatgattagatatacttgcatgttgatttgtataattaaataaattatagattatagatgatgttgtatgcttgcatggggttaactatataattattgctagtgggtaatgatgtggcatggttacatgttgagttttaggagttagtgagcatcaactttatagtaagatatgattattattacctaaacaacatatctcgatcctaaaatatagttatctgtagttgttactctatattttgggacatagaaaataatagcaaagcatttataaacttatttggtatggcatattaatatagtaggatagcaaattgaactagccctaaattagctttgccaatatttaacatcaaactacaaaaaatcattttatcgaatttttgggttgccaatattttgagaagtttataatattggctaaaagccaaacagaaccttagttaatcggaactatatttttcgtgatctaagatagttgttgtagaaatgaatgttgctacacaaatcaagacgctctaggtatatgtgatacaaataacataaaaatatgtcgtcattcgtgctcaggatggttctgttcaaaatatgctggaatttgtcagttccatgcccttcctattagagccgcaagaatgattttttaaaaggacaagaaaagaaccggtttatactgttttttattctgcatttcagttctgggggtgtcgtgcgtttttgtttgctgcaaaaacagccatcctgcataggaaaccagagtttcaaatgcccacagattctaccgagtatagttaattagtggtaaattaattctaactaggacgcaggccctattatcaacgttggcgtcttagcgacaggaggacaaacacggttataggtatgtataccttgtctcaagtaagtccaaagatgtttgttaattgttatgaaatgtaggcatggttgcgatgagattacgagacccatagt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| + | |
| − | Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001062042.1 RefSeq:Os05g0405000]|
| + | ===Expression === |
| − | }}
| + | [[File:Os05g0405000-2.png|right|thumb|250px|'''Figure 3. Expression analysis by RT-PCR and GUS assay.''' '' <ref name="ref1" />.'']] |
| − | [[Category:Genes]] | + | * Expression analyses demonstrated that the cytosolic mRNA of OsPPDKB was induced in the reproductive organs after pollination, and greatly increased until about 10 days after fertilization. |
| − | [[Category:Japonica mRNA]]
| + | * This mRNA was localized mainly in the endosperm, aleurone, and scutellum of the developing kernel. |
| − | [[Category:Oryza Sativa Japonica Group]] | + | |
| − | [[Category:Japonica Genes]]
| + | You can also add sub-section(s) at will. |
| − | [[Category:Japonica Chromosome 5]] | + | |
| − | [[Category:Chromosome 5]]
| + | |
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| | + | |
| | + | ==Labs working on this gene== |
| | + | *Laboratory of Plant Functional Genomics, Division of Molecular and Life Sciences, Pohang University of Science and Technology, Pohang 790-784, Korea, and |
| | + | *Bioscience Center, Nagoya University, Aichi 464-01, Japan |
| | + | ==References== |
| | + | <references> |
| | + | * <ref name="ref1"> |
| | + | Dai X, You C, Wang L, Chen G, Zhang Q, Wu C. Molecular characterization, |
| | + | expression pattern, and function analysis of the OsBC1L family in rice. Plant Mol |
| | + | Biol. 2009 Nov;71(4-5):469-81. doi: 10.1007/s11103-009-9537-3. Epub 2009 Aug 18. |
| | + | PubMed PMID: 19688299. |
| | + | </ref> |
| | + | </references> |
| | + | |
| | + | |
| | + | ==Structured Information== |
| | + | [[Category:Genes]][[Category:Oryza Sativa Japonica Group]][[Category:Japonica Chromosome 5]] |
